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The map label for this gene is ssiE [H]

Identifier: 62389901

GI number: 62389901

Start: 1067579

End: 1069042

Strand: Direct

Name: ssiE [H]

Synonym: cg1151

Alternate gene names: 62389901

Gene position: 1067579-1069042 (Clockwise)

Preceding gene: 62389897

Following gene: 62389902

Centisome position: 32.52

GC content: 53.55

Gene sequence:

>1464_bases
ATGGCCACGGCCGAGAACACAACACAGGAGAATCGGAAAATCCTGTTCAACGCATTTGATATGAACTGCGTTGCGCATCA
GTCCCCAGGACTGTGGACACACCCGAAGGATAAGGCGCGAGACTACAACACTCTTGATTACTGGGTGCACCTTGCCAAGA
CTTTGGAGAAGGGCCTTTTCGACGGCCTTTTCATCGCAGATGTGCTTGGAACTTACGATGTTTATGGTTCTAGTAATGAA
GCGGCGTTGAGCAGTGGTGCGCAGGTGCCTGTCAATGATCCGATCCTTCTTGTTTCTGCGATGGCCTATGCCACAAAGAA
CCTCGGGTTTGGCATTACTGCAGGTACTGCCTATGAGCACCCGTATCCTTTTGCGCGGCGTCTGGCCACACTTGATCACC
TGACTAATGGGCGTGTGGGGTGGAATGTGGTTACTGGCTATCTTCCCTCTGCTGCTCAAAACATGGGTGACACCGATCAG
CTGCCACATGATGAGCGCTATGACAAAGCAGATGAATACCTGGAAGTGATCTACAAGCTTCTCGAGGGCTCCTGGGAAGA
CGATGCTGTTCAAAACAATACGGAGACGAGTGTCTTTACGGACTCCTCCAAAGTGCACGCCATTAATCATCATGGCAAGT
ACTTTGATGTGCCGGGCATTGCCATCACTGAGCCGAGTGTGCAGCGTACGCCGGTGATCTACCAGGCGGGTGCATCGCCG
CGCGGATTGAAATTCGCTGGTGAGAATGCAGAAGCAGTGTTTATCAATTCCAGCACCGTGGAGGCAATCACCAAGACTGT
CGCAAAAATTCGCGCTGCTGCGGTCGCTGCGGGACGTGATCCACATGCGGTGAAGATCTTTGCGATGCAAACCATCATCA
CTGGTGAAACAGAAGCAGATGCGCAGGCAAAGCTGGAGGAATACAGTCGCTATATCGATCCTGTCGGTGGTCTGACCTTG
ATGTCTGGATGGACCGGCGCGGATCTGTCGCAGTATGACCTGGATGAACCGATCACCAATATTGAGTCAAACGCTATTCA
GTCCACTGCAGCCACCATTAGCAACGGCACCGGTGAAGGTGCGTGGACGGTACGCAAACTGGGTGAGGCAACCGGCATCG
GCGGCTTCGGACCAGTGCTTGTGGGATCTGGCGCTAACGTTGCCGCGGAACTTGCACGCATCCAGGATCTCAGCGATGTT
GATGGTTTCAACCTTGCTTATGCCATCACCCCAGGAACTTTTGAAGATGTCGTGGACTTTGTGGTGCCTGAGCTGCAAAA
ACTTAGCCGCTACAAGACGGAATACGCGCCGGGTTCCTTGCGCAACAAATTGCTCGGTAAAGGTGATCGCCTGGACGATA
CCCACCGCGGCGCAAGCTACCGCCTAGGCGCTCGGAACTCCACCGCCACTATTGATCTCAGTTCCATATCCGCCCAACTA
GTTTCCCAGGGAGCCCACTCATGA

Upstream 100 bases:

>100_bases
TTCATTCAGGGTGAATGCTCTCCTTGTTTCAGATGTTCAACGCTCCATAAAGTAGACCGCAATCTAGACAAAGATGTCTA
TTTTAATTAAGGAGCAGAAC

Downstream 100 bases:

>100_bases
TCTCACCGCAAACAATCATCGACAATCTTGCTCCAGTCCTCGCGGAGATCGCAGCAACGGCTGCGCAGCGCGAACAGGAT
CGAGAATTCAGCCGTGATTT

Product: monooxygenase

Products: an aldehyde; SO3; H2O; FMN [C]

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MATAENTTQENRKILFNAFDMNCVAHQSPGLWTHPKDKARDYNTLDYWVHLAKTLEKGLFDGLFIADVLGTYDVYGSSNE
AALSSGAQVPVNDPILLVSAMAYATKNLGFGITAGTAYEHPYPFARRLATLDHLTNGRVGWNVVTGYLPSAAQNMGDTDQ
LPHDERYDKADEYLEVIYKLLEGSWEDDAVQNNTETSVFTDSSKVHAINHHGKYFDVPGIAITEPSVQRTPVIYQAGASP
RGLKFAGENAEAVFINSSTVEAITKTVAKIRAAAVAAGRDPHAVKIFAMQTIITGETEADAQAKLEEYSRYIDPVGGLTL
MSGWTGADLSQYDLDEPITNIESNAIQSTAATISNGTGEGAWTVRKLGEATGIGGFGPVLVGSGANVAAELARIQDLSDV
DGFNLAYAITPGTFEDVVDFVVPELQKLSRYKTEYAPGSLRNKLLGKGDRLDDTHRGASYRLGARNSTATIDLSSISAQL
VSQGAHS

Sequences:

>Translated_487_residues
MATAENTTQENRKILFNAFDMNCVAHQSPGLWTHPKDKARDYNTLDYWVHLAKTLEKGLFDGLFIADVLGTYDVYGSSNE
AALSSGAQVPVNDPILLVSAMAYATKNLGFGITAGTAYEHPYPFARRLATLDHLTNGRVGWNVVTGYLPSAAQNMGDTDQ
LPHDERYDKADEYLEVIYKLLEGSWEDDAVQNNTETSVFTDSSKVHAINHHGKYFDVPGIAITEPSVQRTPVIYQAGASP
RGLKFAGENAEAVFINSSTVEAITKTVAKIRAAAVAAGRDPHAVKIFAMQTIITGETEADAQAKLEEYSRYIDPVGGLTL
MSGWTGADLSQYDLDEPITNIESNAIQSTAATISNGTGEGAWTVRKLGEATGIGGFGPVLVGSGANVAAELARIQDLSDV
DGFNLAYAITPGTFEDVVDFVVPELQKLSRYKTEYAPGSLRNKLLGKGDRLDDTHRGASYRLGARNSTATIDLSSISAQL
VSQGAHS
>Mature_486_residues
ATAENTTQENRKILFNAFDMNCVAHQSPGLWTHPKDKARDYNTLDYWVHLAKTLEKGLFDGLFIADVLGTYDVYGSSNEA
ALSSGAQVPVNDPILLVSAMAYATKNLGFGITAGTAYEHPYPFARRLATLDHLTNGRVGWNVVTGYLPSAAQNMGDTDQL
PHDERYDKADEYLEVIYKLLEGSWEDDAVQNNTETSVFTDSSKVHAINHHGKYFDVPGIAITEPSVQRTPVIYQAGASPR
GLKFAGENAEAVFINSSTVEAITKTVAKIRAAAVAAGRDPHAVKIFAMQTIITGETEADAQAKLEEYSRYIDPVGGLTLM
SGWTGADLSQYDLDEPITNIESNAIQSTAATISNGTGEGAWTVRKLGEATGIGGFGPVLVGSGANVAAELARIQDLSDVD
GFNLAYAITPGTFEDVVDFVVPELQKLSRYKTEYAPGSLRNKLLGKGDRLDDTHRGASYRLGARNSTATIDLSSISAQLV
SQGAHS

Specific function: Part of a pathway to remove covalently bound sulfur from dibenzothiophene (DBT) without breaking carbon-carbon bonds. This enzyme metabolizes DBT-sulfone (DBTO2 or DBT 5,5-dioxide) to 2- hydroxybiphenyl (2-HBP) [H]

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ntaA/snaA/soxA(dszA) monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1787166, Length=354, Percent_Identity=25.1412429378531, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011251
- InterPro:   IPR016048
- InterPro:   IPR016215 [H]

Pfam domain/function: PF00296 Bac_luciferase [H]

EC number: 1.14.14.5 [C]

Molecular weight: Translated: 52410; Mature: 52279

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATAENTTQENRKILFNAFDMNCVAHQSPGLWTHPKDKARDYNTLDYWVHLAKTLEKGLF
CCCCCCCCCCCCEEEEEEECCCEEEECCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHH
DGLFIADVLGTYDVYGSSNEAALSSGAQVPVNDPILLVSAMAYATKNLGFGITAGTAYEH
CHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCC
PYPFARRLATLDHLTNGRVGWNVVTGYLPSAAQNMGDTDQLPHDERYDKADEYLEVIYKL
CCHHHHHHHHHHHHCCCCCCEEHHCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
LEGSWEDDAVQNNTETSVFTDSSKVHAINHHGKYFDVPGIAITEPSVQRTPVIYQAGASP
HCCCCCCCHHCCCCCCEEEECCCCEEEECCCCCEEECCCEEECCCCCCCCCEEEECCCCC
RGLKFAGENAEAVFINSSTVEAITKTVAKIRAAAVAAGRDPHAVKIFAMQTIITGETEAD
CCEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCH
AQAKLEEYSRYIDPVGGLTLMSGWTGADLSQYDLDEPITNIESNAIQSTAATISNGTGEG
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCC
AWTVRKLGEATGIGGFGPVLVGSGANVAAELARIQDLSDVDGFNLAYAITPGTFEDVVDF
CHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHH
VVPELQKLSRYKTEYAPGSLRNKLLGKGDRLDDTHRGASYRLGARNSTATIDLSSISAQL
HHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCEEECCCCCCEEEEHHHHHHHH
VSQGAHS
HHCCCCC
>Mature Secondary Structure 
ATAENTTQENRKILFNAFDMNCVAHQSPGLWTHPKDKARDYNTLDYWVHLAKTLEKGLF
CCCCCCCCCCCEEEEEEECCCEEEECCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHH
DGLFIADVLGTYDVYGSSNEAALSSGAQVPVNDPILLVSAMAYATKNLGFGITAGTAYEH
CHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCC
PYPFARRLATLDHLTNGRVGWNVVTGYLPSAAQNMGDTDQLPHDERYDKADEYLEVIYKL
CCHHHHHHHHHHHHCCCCCCEEHHCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
LEGSWEDDAVQNNTETSVFTDSSKVHAINHHGKYFDVPGIAITEPSVQRTPVIYQAGASP
HCCCCCCCHHCCCCCCEEEECCCCEEEECCCCCEEECCCEEECCCCCCCCCEEEECCCCC
RGLKFAGENAEAVFINSSTVEAITKTVAKIRAAAVAAGRDPHAVKIFAMQTIITGETEAD
CCEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCH
AQAKLEEYSRYIDPVGGLTLMSGWTGADLSQYDLDEPITNIESNAIQSTAATISNGTGEG
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCC
AWTVRKLGEATGIGGFGPVLVGSGANVAAELARIQDLSDVDGFNLAYAITPGTFEDVVDF
CHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHH
VVPELQKLSRYKTEYAPGSLRNKLLGKGDRLDDTHRGASYRLGARNSTATIDLSSISAQL
HHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCEEECCCCCCEEEEHHHHHHHH
VSQGAHS
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: an alkanesulfonate; O2; FMNH2 [C]

Specific reaction: an alkanesulfonate + O2 + FMNH2 = an aldehyde + SO3 + H2O + FMN [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961424; 7574582 [H]