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The map label for this gene is ispE [H]

Identifier: 62389801

GI number: 62389801

Start: 967322

End: 968257

Strand: Direct

Name: ispE [H]

Synonym: cg1039

Alternate gene names: 62389801

Gene position: 967322-968257 (Clockwise)

Preceding gene: 62389800

Following gene: 62389802

Centisome position: 29.47

GC content: 60.36

Gene sequence:

>936_bases
GTGAAAATTACCGCTAAGGCGTGGGCGAAAACCAACCTGCATTTAGGTGTGGGACCGGCTCACGACGATGGATTTCACGA
GCTCATGACGGTGTTTCAAACCATTGATCTGTTTGACACCGTCACCTTAACCACCCTCGATGAGGAGTTGGTGGAGGAGG
GGAGCGTCGTCAAGCAATTATCTGTGACCGGTGCCCGTGGCGTGCCTGAGGACGCCAGCAATCTTGCGTGGCGCGCTGTG
GATGCGTTGGTTAAGCGGCGCGCGGAAAAGACGCCGCTGTCTGCAGTTTCGCTGCATATTTCCAAGGGGATTCCGGTGGC
TGGCGGCATGGCTGGCGGCTCTGCGGATGCGGCTGCGACACTGCGCGCAGTGGATGCCTGGATTGGGCCTTTCGGCGAGG
ACACATTGCTGGAGGTTGCCGCGGAGCTCGGCTCAGATGTGCCGTTTTGCCTGCTTGGTGGCACCATGCGCGGTACCGGT
CGCGGCGAGCAGCTGGTAGATATGTTGACGCGCGGCAAGCTACATTGGGTGGTGGCCGCGATGGCGCATGGCCTGTCCAC
GCCTGAGGTATTCAAAAAGCATGATGAGCTGAATCCGGAATCGCATATGGATATCAGCGACCTCAGCGCCGCACTTCTCA
CCGGCAACACCGCCGAGGTGGGGCAGTGGCTGCACAATGATCTGACCAGCGCCGCACTCAGTTTGCGCCCTGAACTGCGC
AGCGTCCTCCAAGAAGGCATCCGCTCCGGCGCGCATGCAGGAATTGTCTCCGGCTCCGGCCCGACCACGGTATTCTTGTG
CGAATCGGAGCACAAAGCGCAAGACGTTAAAGAGGCGCTAATCGACGCCGGCCAGGTGTACGCTGCTTACACCGCCACCG
GCCCTGCGGCCTCAACCGCCGACCAGCGCGGCGCACACATTTTGACTGTTTCATAA

Upstream 100 bases:

>100_bases
CTTTGCGGGCCGCTGATATTGATCCAACGCTTCGTGGCGAAAAGCTTGATGTCACTGACTATGTGCGCCTAGCTGGGGTG
TTGCAGCAAAAGGATGAGAA

Downstream 100 bases:

>100_bases
TAAAGACAAACTTAAGTATCGGAGTCGAAGAAAAACCACAATGGCCAATCTGATTAATCTCGAGAACGTCTCCAAAACCT
GGGGATTAAAAACGCTTCTC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKITAKAWAKTNLHLGVGPAHDDGFHELMTVFQTIDLFDTVTLTTLDEELVEEGSVVKQLSVTGARGVPEDASNLAWRAV
DALVKRRAEKTPLSAVSLHISKGIPVAGGMAGGSADAAATLRAVDAWIGPFGEDTLLEVAAELGSDVPFCLLGGTMRGTG
RGEQLVDMLTRGKLHWVVAAMAHGLSTPEVFKKHDELNPESHMDISDLSAALLTGNTAEVGQWLHNDLTSAALSLRPELR
SVLQEGIRSGAHAGIVSGSGPTTVFLCESEHKAQDVKEALIDAGQVYAAYTATGPAASTADQRGAHILTVS

Sequences:

>Translated_311_residues
MKITAKAWAKTNLHLGVGPAHDDGFHELMTVFQTIDLFDTVTLTTLDEELVEEGSVVKQLSVTGARGVPEDASNLAWRAV
DALVKRRAEKTPLSAVSLHISKGIPVAGGMAGGSADAAATLRAVDAWIGPFGEDTLLEVAAELGSDVPFCLLGGTMRGTG
RGEQLVDMLTRGKLHWVVAAMAHGLSTPEVFKKHDELNPESHMDISDLSAALLTGNTAEVGQWLHNDLTSAALSLRPELR
SVLQEGIRSGAHAGIVSGSGPTTVFLCESEHKAQDVKEALIDAGQVYAAYTATGPAASTADQRGAHILTVS
>Mature_311_residues
MKITAKAWAKTNLHLGVGPAHDDGFHELMTVFQTIDLFDTVTLTTLDEELVEEGSVVKQLSVTGARGVPEDASNLAWRAV
DALVKRRAEKTPLSAVSLHISKGIPVAGGMAGGSADAAATLRAVDAWIGPFGEDTLLEVAAELGSDVPFCLLGGTMRGTG
RGEQLVDMLTRGKLHWVVAAMAHGLSTPEVFKKHDELNPESHMDISDLSAALLTGNTAEVGQWLHNDLTSAALSLRPELR
SVLQEGIRSGAHAGIVSGSGPTTVFLCESEHKAQDVKEALIDAGQVYAAYTATGPAASTADQRGAHILTVS

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=196, Percent_Identity=32.6530612244898, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 32630; Mature: 32630

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITAKAWAKTNLHLGVGPAHDDGFHELMTVFQTIDLFDTVTLTTLDEELVEEGSVVKQL
CCCCCCHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SVTGARGVPEDASNLAWRAVDALVKRRAEKTPLSAVSLHISKGIPVAGGMAGGSADAAAT
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHEEEEHHCCCCCCCCCCCCCCHHHHH
LRAVDAWIGPFGEDTLLEVAAELGSDVPFCLLGGTMRGTGRGEQLVDMLTRGKLHWVVAA
HHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHH
MAHGLSTPEVFKKHDELNPESHMDISDLSAALLTGNTAEVGQWLHNDLTSAALSLRPELR
HHHCCCCHHHHHHHHCCCCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHH
SVLQEGIRSGAHAGIVSGSGPTTVFLCESEHKAQDVKEALIDAGQVYAAYTATGPAASTA
HHHHHHHHCCCCCCEEECCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCH
DQRGAHILTVS
HHCCCEEEEEC
>Mature Secondary Structure
MKITAKAWAKTNLHLGVGPAHDDGFHELMTVFQTIDLFDTVTLTTLDEELVEEGSVVKQL
CCCCCCHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SVTGARGVPEDASNLAWRAVDALVKRRAEKTPLSAVSLHISKGIPVAGGMAGGSADAAAT
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHEEEEHHCCCCCCCCCCCCCCHHHHH
LRAVDAWIGPFGEDTLLEVAAELGSDVPFCLLGGTMRGTGRGEQLVDMLTRGKLHWVVAA
HHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHH
MAHGLSTPEVFKKHDELNPESHMDISDLSAALLTGNTAEVGQWLHNDLTSAALSLRPELR
HHHCCCCHHHHHHHHCCCCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHH
SVLQEGIRSGAHAGIVSGSGPTTVFLCESEHKAQDVKEALIDAGQVYAAYTATGPAASTA
HHHHHHHHCCCCCCEEECCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCH
DQRGAHILTVS
HHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA