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The map label for this gene is kpnIM [H]

Identifier: 62389793

GI number: 62389793

Start: 959310

End: 960467

Strand: Reverse

Name: kpnIM [H]

Synonym: cg1028

Alternate gene names: 62389793

Gene position: 960467-959310 (Counterclockwise)

Preceding gene: 62389804

Following gene: 62389792

Centisome position: 29.26

GC content: 60.36

Gene sequence:

>1158_bases
GTGTCGAGACTGACGGAACTGCTGCGGCAGGTGCGCAAGGCGGACGCGCAACTTGGTACTGACCTGGAAGCCGAGGTCGC
TGCGCTGACCAAGCGTCGCACCTTCGGGCTCGTCTTTGAGCAGCATCAGCCTGAGGCTGTCGAGCTGCCCGGCAGGGTCG
TCCGTCGCGGCGACAAGGTGCGGGTGCTGCCTCCGCGAGGGGGGACAAAGGCAGGTGACCAACGGCTGTGGCGGACAACT
CGGATCGAGTGCGTCGACGGGCAGCGTGTGGCTCATCTCGCGGAGCTCGACGTCGAAGAACCCGAGACTCGGGCAGTGCT
TGCCGACGACGTGGTGGTCGTCGCGGAGTTCCGGGATCGCATCTACCCCGGCCTGGTGGAGACAGGCAGGGTTGAGCGGG
GCGGCGACAAGCCGTTCCACACGGTCGTCAACGCTGAGAACTACCACGCGCTGGAGATGCTGACCTATACGCACCGGCAT
TCCATCGACGCCATCTACATCGACCCGCCGTACAACACCGGGGCGAGGGACTGGAAGTACGACAACGATTACGTCGCGAG
TGATGACGACTATCGACACTCGAAATGGCTGGCGTTCATGGAGCGACGGTTGAAGATCTGTCGGGAGCTCATGCGTAGCG
ATGCTACTCTTGTGGCAACTATCGATGAGCATGAAGTAAACCGTTTGGGCGTGTTGCTAGATCAGCTCTTCCCGGAATCT
ACGCGGCAACTCGTCACAATTGTCAACAACCCTAAAGGCGTTACTCAGGGATATCTTTCGAGGGTCGAAGAGTATGCGTT
CTTTGTATTTGGTCCTGACGCGCGAATCGGTTCGGTCGATGACGACCTTCTGACGCATCGAGACATGGCCGATGCTGAAG
GGGAACTGCAGAGGCCTCGATGGAAGGGGCTCTTGCGGTCGGGCGACGACTCGCTTCGAGCTGACCGTAAAGATATGTTC
TATCCGGTGTGGTTCGATGAGTCGACTGGGCGACTCAGCCACGCGGGCGAAGCATTGCCACTTGACGAAACTCCTGACTT
CAGTCCGCAGGATGGCCTGACGCCGATCTGGCCTATTAGGCGGGACATGAAGGAGGGGCCTACCCGGGCAGCGCCACGCC
GTTCGATCCTTGACTACGCGCTACACCCTCATCTGTGA

Upstream 100 bases:

>100_bases
AGGTTGAAGGATCACGTGGTGGAGTTCCGGACGGGACTTACGTCTGGAAGTAAGAAATAGCCCGGACGCGCTACGGATGA
TGACGAAGGAGCCCCTATCC

Downstream 100 bases:

>100_bases
AGAGCCACCAAACCTGGGACGGTTCAAGCGGCGCTAGGCGAGTCTGATTGCTGCGTCGATCTATGGGGTGAGGGCAGTGG
CCGACATCGCGGTCCGAACT

Product: restriction-modification system: methylase

Products: NA

Alternate protein names: M.KpnI; Adenine-specific methyltransferase KpnI [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MSRLTELLRQVRKADAQLGTDLEAEVAALTKRRTFGLVFEQHQPEAVELPGRVVRRGDKVRVLPPRGGTKAGDQRLWRTT
RIECVDGQRVAHLAELDVEEPETRAVLADDVVVVAEFRDRIYPGLVETGRVERGGDKPFHTVVNAENYHALEMLTYTHRH
SIDAIYIDPPYNTGARDWKYDNDYVASDDDYRHSKWLAFMERRLKICRELMRSDATLVATIDEHEVNRLGVLLDQLFPES
TRQLVTIVNNPKGVTQGYLSRVEEYAFFVFGPDARIGSVDDDLLTHRDMADAEGELQRPRWKGLLRSGDDSLRADRKDMF
YPVWFDESTGRLSHAGEALPLDETPDFSPQDGLTPIWPIRRDMKEGPTRAAPRRSILDYALHPHL

Sequences:

>Translated_385_residues
MSRLTELLRQVRKADAQLGTDLEAEVAALTKRRTFGLVFEQHQPEAVELPGRVVRRGDKVRVLPPRGGTKAGDQRLWRTT
RIECVDGQRVAHLAELDVEEPETRAVLADDVVVVAEFRDRIYPGLVETGRVERGGDKPFHTVVNAENYHALEMLTYTHRH
SIDAIYIDPPYNTGARDWKYDNDYVASDDDYRHSKWLAFMERRLKICRELMRSDATLVATIDEHEVNRLGVLLDQLFPES
TRQLVTIVNNPKGVTQGYLSRVEEYAFFVFGPDARIGSVDDDLLTHRDMADAEGELQRPRWKGLLRSGDDSLRADRKDMF
YPVWFDESTGRLSHAGEALPLDETPDFSPQDGLTPIWPIRRDMKEGPTRAAPRRSILDYALHPHL
>Mature_384_residues
SRLTELLRQVRKADAQLGTDLEAEVAALTKRRTFGLVFEQHQPEAVELPGRVVRRGDKVRVLPPRGGTKAGDQRLWRTTR
IECVDGQRVAHLAELDVEEPETRAVLADDVVVVAEFRDRIYPGLVETGRVERGGDKPFHTVVNAENYHALEMLTYTHRHS
IDAIYIDPPYNTGARDWKYDNDYVASDDDYRHSKWLAFMERRLKICRELMRSDATLVATIDEHEVNRLGVLLDQLFPEST
RQLVTIVNNPKGVTQGYLSRVEEYAFFVFGPDARIGSVDDDLLTHRDMADAEGELQRPRWKGLLRSGDDSLRADRKDMFY
PVWFDESTGRLSHAGEALPLDETPDFSPQDGLTPIWPIRRDMKEGPTRAAPRRSILDYALHPHL

Specific function: This methylase recognizes the double-stranded sequence GGTACC, causes specific methylation on A-4 on both strands, and protects the DNA from cleavage by the KpnI endonuclease [H]

COG id: COG2189

COG function: function code L; Adenine specific DNA methylase Mod

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002295
- InterPro:   IPR002941
- InterPro:   IPR002052 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 44023; Mature: 43892

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLTELLRQVRKADAQLGTDLEAEVAALTKRRTFGLVFEQHQPEAVELPGRVVRRGDKV
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHCCCEE
RVLPPRGGTKAGDQRLWRTTRIECVDGQRVAHLAELDVEEPETRAVLADDVVVVAEFRDR
EEECCCCCCCCHHHHHHHHHEEEEECCHHHHHHHHCCCCCCCCCEEECCCEEEEEHHHHH
IYPGLVETGRVERGGDKPFHTVVNAENYHALEMLTYTHRHSIDAIYIDPPYNTGARDWKY
HCCCCHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCC
DNDYVASDDDYRHSKWLAFMERRLKICRELMRSDATLVATIDEHEVNRLGVLLDQLFPES
CCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCC
TRQLVTIVNNPKGVTQGYLSRVEEYAFFVFGPDARIGSVDDDLLTHRDMADAEGELQRPR
CCEEEEEECCCCCHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCCH
WKGLLRSGDDSLRADRKDMFYPVWFDESTGRLSHAGEALPLDETPDFSPQDGLTPIWPIR
HHHHHHCCCHHHHCCHHHCEEEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
RDMKEGPTRAAPRRSILDYALHPHL
HHHHCCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
SRLTELLRQVRKADAQLGTDLEAEVAALTKRRTFGLVFEQHQPEAVELPGRVVRRGDKV
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHCCCEE
RVLPPRGGTKAGDQRLWRTTRIECVDGQRVAHLAELDVEEPETRAVLADDVVVVAEFRDR
EEECCCCCCCCHHHHHHHHHEEEEECCHHHHHHHHCCCCCCCCCEEECCCEEEEEHHHHH
IYPGLVETGRVERGGDKPFHTVVNAENYHALEMLTYTHRHSIDAIYIDPPYNTGARDWKY
HCCCCHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCC
DNDYVASDDDYRHSKWLAFMERRLKICRELMRSDATLVATIDEHEVNRLGVLLDQLFPES
CCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCC
TRQLVTIVNNPKGVTQGYLSRVEEYAFFVFGPDARIGSVDDDLLTHRDMADAEGELQRPR
CCEEEEEECCCCCHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCCH
WKGLLRSGDDSLRADRKDMFYPVWFDESTGRLSHAGEALPLDETPDFSPQDGLTPIWPIR
HHHHHHCCCHHHHCCHHHCEEEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
RDMKEGPTRAAPRRSILDYALHPHL
HHHHCCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1754388 [H]