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The map label for this gene is thyA [H]

Identifier: 62389734

GI number: 62389734

Start: 899902

End: 900702

Strand: Reverse

Name: thyA [H]

Synonym: cg0966

Alternate gene names: 62389734

Gene position: 900702-899902 (Counterclockwise)

Preceding gene: 62389735

Following gene: 62389733

Centisome position: 27.44

GC content: 53.18

Gene sequence:

>801_bases
ATGACTGTTCCAACGCCTTATGAAGACCTTCTTCGGAAGATTGCTGAAGAAGGGTCCCACAAGGACGACCGCACCGGCAC
CGGCACTACTTCTTTATTCGGACAACAAATCCGCTTTGATCTCAATGAAGGTTTTCCCCTTCTGACCACCAAGAAGGTCC
ATTTCCACTCTGTTGTGGGTGAGCTTTTGTGGTTCCTTCAGGGGGATTCCAACGTCAAATGGCTGCAGGATAACAACATC
CGCATTTGGAATGAATGGGCAGATGAGGACGGCGAGCTGGGCCCTGTTTATGGTGTCCAGTGGCGTTCTTGGCCAACCCC
TGATGGTCGTCACATTGACCAGATCTCAGGTGCTTTAGAAACTCTGCGAAACAACCCTGATTCACGTCGCAATATTGTCT
CGGCGTGGAATGTTTCCGAGCTTGAAAACATGGCTCTTCCCCCTTGTCACTTGCTTTTCCAGCTCTATGTCGCCGATGGC
AAACTGTCTTGCCAGCTCTACCAGCGTTCTGCGGACATGTTCCTGGGTGTGCCTTTCAACATCGCATCTTATGCACTGCT
CACCCACATGTTTGCCCAGCAGGCAGGCTTGGAAGTCGGCGAGTTCATTTGGACTGGCGGCGACTGCCACATTTATGACA
ACCACAAGGAACAGGTCGCGGAGCAGCTGAGCCGCGAAGCTCGCCCCTACCCCACCTTGGAGCTCAACAAGGCAGCGTCC
ATGTTTGAGTACAGCTTCGATGACATCACCGTGTCCGGCTACGATCCACACCCATTGATCCGCGGCAAGGTCGCCGTATG
A

Upstream 100 bases:

>100_bases
CATCTTGATCTGTCGCCCTGAACTTGCAGATGAACTTCTCGAGATGTGCGCGAAGTTCTACGAGGAGAATGGAACTTACT
AACGCTGTTATGATGACGGC

Downstream 100 bases:

>100_bases
TCGGTGCGATTTGGGCACAAGGCCGCGACGGCATCATCGGCGACGGCACCGACATGCCCTGGCACATCCCGGAAGACCTC
AAACACTTCAAGAAAACCAC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MTVPTPYEDLLRKIAEEGSHKDDRTGTGTTSLFGQQIRFDLNEGFPLLTTKKVHFHSVVGELLWFLQGDSNVKWLQDNNI
RIWNEWADEDGELGPVYGVQWRSWPTPDGRHIDQISGALETLRNNPDSRRNIVSAWNVSELENMALPPCHLLFQLYVADG
KLSCQLYQRSADMFLGVPFNIASYALLTHMFAQQAGLEVGEFIWTGGDCHIYDNHKEQVAEQLSREARPYPTLELNKAAS
MFEYSFDDITVSGYDPHPLIRGKVAV

Sequences:

>Translated_266_residues
MTVPTPYEDLLRKIAEEGSHKDDRTGTGTTSLFGQQIRFDLNEGFPLLTTKKVHFHSVVGELLWFLQGDSNVKWLQDNNI
RIWNEWADEDGELGPVYGVQWRSWPTPDGRHIDQISGALETLRNNPDSRRNIVSAWNVSELENMALPPCHLLFQLYVADG
KLSCQLYQRSADMFLGVPFNIASYALLTHMFAQQAGLEVGEFIWTGGDCHIYDNHKEQVAEQLSREARPYPTLELNKAAS
MFEYSFDDITVSGYDPHPLIRGKVAV
>Mature_265_residues
TVPTPYEDLLRKIAEEGSHKDDRTGTGTTSLFGQQIRFDLNEGFPLLTTKKVHFHSVVGELLWFLQGDSNVKWLQDNNIR
IWNEWADEDGELGPVYGVQWRSWPTPDGRHIDQISGALETLRNNPDSRRNIVSAWNVSELENMALPPCHLLFQLYVADGK
LSCQLYQRSADMFLGVPFNIASYALLTHMFAQQAGLEVGEFIWTGGDCHIYDNHKEQVAEQLSREARPYPTLELNKAASM
FEYSFDDITVSGYDPHPLIRGKVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=281, Percent_Identity=48.0427046263345, Blast_Score=261, Evalue=4e-70,
Organism=Escherichia coli, GI1789191, Length=261, Percent_Identity=64.7509578544061, Blast_Score=378, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71993377, Length=282, Percent_Identity=44.3262411347518, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6324648, Length=278, Percent_Identity=37.410071942446, Blast_Score=181, Evalue=1e-46,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=50.1766784452297, Blast_Score=271, Evalue=5e-73,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 30230; Mature: 30099

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPTPYEDLLRKIAEEGSHKDDRTGTGTTSLFGQQIRFDLNEGFPLLTTKKVHFHSVVG
CCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHEEEECCCCCCEEEECHHHHHHHHH
ELLWFLQGDSNVKWLQDNNIRIWNEWADEDGELGPVYGVQWRSWPTPDGRHIDQISGALE
HHHHHHCCCCCEEEEECCCEEEEECCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHH
TLRNNPDSRRNIVSAWNVSELENMALPPCHLLFQLYVADGKLSCQLYQRSADMFLGVPFN
HHHCCCCHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHCCCCEEEHEECCCCCEEEECCHH
IASYALLTHMFAQQAGLEVGEFIWTGGDCHIYDNHKEQVAEQLSREARPYPTLELNKAAS
HHHHHHHHHHHHHHHCCCHHHEEEECCEEEEECCHHHHHHHHHHHHCCCCCCEEHHHHHH
MFEYSFDDITVSGYDPHPLIRGKVAV
HHEECCCCEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVPTPYEDLLRKIAEEGSHKDDRTGTGTTSLFGQQIRFDLNEGFPLLTTKKVHFHSVVG
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHEEEECCCCCCEEEECHHHHHHHHH
ELLWFLQGDSNVKWLQDNNIRIWNEWADEDGELGPVYGVQWRSWPTPDGRHIDQISGALE
HHHHHHCCCCCEEEEECCCEEEEECCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHH
TLRNNPDSRRNIVSAWNVSELENMALPPCHLLFQLYVADGKLSCQLYQRSADMFLGVPFN
HHHCCCCHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHCCCCEEEHEECCCCCEEEECCHH
IASYALLTHMFAQQAGLEVGEFIWTGGDCHIYDNHKEQVAEQLSREARPYPTLELNKAAS
HHHHHHHHHHHHHHHCCCHHHEEEECCEEEEECCHHHHHHHHHHHHCCCCCCEEHHHHHH
MFEYSFDDITVSGYDPHPLIRGKVAV
HHEECCCCEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]