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The map label for this gene is folA [H]

Identifier: 62389733

GI number: 62389733

Start: 899447

End: 899905

Strand: Reverse

Name: folA [H]

Synonym: cg0965

Alternate gene names: 62389733

Gene position: 899905-899447 (Counterclockwise)

Preceding gene: 62389734

Following gene: 62389732

Centisome position: 27.41

GC content: 58.82

Gene sequence:

>459_bases
ATGATCGGTGCGATTTGGGCACAAGGCCGCGACGGCATCATCGGCGACGGCACCGACATGCCCTGGCACATCCCGGAAGA
CCTCAAACACTTCAAGAAAACCACCATGGGCCAGCCGGTCATCATGGGTCGTCGCACGTGGGAGTCTTTGCCGTTCAAGC
CGCTTCCCGGCCGCGAGAACTTCATTCTCTCCTCACGCGAGCCCGGCGACTGGTCCGCCGGCGGCACAGTGGTCACCGAA
ATCCCTAAAAGCGGCTGGATCATGGGCGGCGGCGAGGTCTACAAGGCCACCGTCGGCAGCGCCGACGTTTTAGAAATAAC
GCTTATCGACGCCACCTTCGATGTTTCCACTCCCGTCTACGCACCCGAAATCCCGGCGAACTTCAACCTCGATGACGAAT
CCGAGTGGTTTACCTCAGGCGAGTATCGTTACAAGTTCCAGCGCTACATCAAGGTTTAA

Upstream 100 bases:

>100_bases
TGGAGCTCAACAAGGCAGCGTCCATGTTTGAGTACAGCTTCGATGACATCACCGTGTCCGGCTACGATCCACACCCATTG
ATCCGCGGCAAGGTCGCCGT

Downstream 100 bases:

>100_bases
GGAGCAAACAACATGAGCAACGTAACCATTTACGCCACAGATTGGTGCCCTTACTGCCGATCCCTCCTCAAAGGTCTCGA
CGGCCAAGAGTACGACCTCA

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MIGAIWAQGRDGIIGDGTDMPWHIPEDLKHFKKTTMGQPVIMGRRTWESLPFKPLPGRENFILSSREPGDWSAGGTVVTE
IPKSGWIMGGGEVYKATVGSADVLEITLIDATFDVSTPVYAPEIPANFNLDDESEWFTSGEYRYKFQRYIKV

Sequences:

>Translated_152_residues
MIGAIWAQGRDGIIGDGTDMPWHIPEDLKHFKKTTMGQPVIMGRRTWESLPFKPLPGRENFILSSREPGDWSAGGTVVTE
IPKSGWIMGGGEVYKATVGSADVLEITLIDATFDVSTPVYAPEIPANFNLDDESEWFTSGEYRYKFQRYIKV
>Mature_152_residues
MIGAIWAQGRDGIIGDGTDMPWHIPEDLKHFKKTTMGQPVIMGRRTWESLPFKPLPGRENFILSSREPGDWSAGGTVVTE
IPKSGWIMGGGEVYKATVGSADVLEITLIDATFDVSTPVYAPEIPANFNLDDESEWFTSGEYRYKFQRYIKV

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=132, Percent_Identity=41.6666666666667, Blast_Score=84, Evalue=3e-18,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 16986; Mature: 16986

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGAIWAQGRDGIIGDGTDMPWHIPEDLKHFKKTTMGQPVIMGRRTWESLPFKPLPGREN
CCCEEECCCCCCEEECCCCCCEECHHHHHHHHHHCCCCCEEECCCCHHCCCCCCCCCCCC
FILSSREPGDWSAGGTVVTEIPKSGWIMGGGEVYKATVGSADVLEITLIDATFDVSTPVY
EEEECCCCCCCCCCCEEEEECCCCCEEEECCEEEEEECCCCCEEEEEEEEEEECCCCCCC
APEIPANFNLDDESEWFTSGEYRYKFQRYIKV
CCCCCCCCCCCCCCCCEECCCEEEEEEEEECC
>Mature Secondary Structure
MIGAIWAQGRDGIIGDGTDMPWHIPEDLKHFKKTTMGQPVIMGRRTWESLPFKPLPGREN
CCCEEECCCCCCEEECCCCCCEECHHHHHHHHHHCCCCCEEECCCCHHCCCCCCCCCCCC
FILSSREPGDWSAGGTVVTEIPKSGWIMGGGEVYKATVGSADVLEITLIDATFDVSTPVY
EEEECCCCCCCCCCCEEEEECCCCCEEEECCEEEEEECCCCCEEEEEEEEEEECCCCCCC
APEIPANFNLDDESEWFTSGEYRYKFQRYIKV
CCCCCCCCCCCCCCCCEECCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]