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The map label for this gene is prpB1

Identifier: 62389584

GI number: 62389584

Start: 715704

End: 716621

Strand: Direct

Name: prpB1

Synonym: cg0797

Alternate gene names: 62389584

Gene position: 715704-716621 (Clockwise)

Preceding gene: 62389583

Following gene: 62389585

Centisome position: 21.8

GC content: 58.71

Gene sequence:

>918_bases
ATGAATCTCTTTTCGAATGGTGTTGATGTGGGGAGGCGTCGACAAGCATTTAAAGCGGCACTCGCCGCACCCCACATCGC
CCGGCTGCCCGGCGCATTCTCCCCTCTGATTGCGCGCTCCATCGAAGAAGCCGGCTTCGAAGGCGTCTACGTTTCCGGCG
CCGTCATAGCTGCTGACCTGGCACTACCCGATATCGGCTTGACGACGCTGACCGAAGTCGCCCACCGCGCGCGGCAAATT
GCGCGCGTCACAGACCTAGGAGTGCTTGTCGACGCCGACACCGGCTTTGGCGAACCCATGTCGGCCGCACGCACCGTCGC
CGAATTGGAGGACGCCGGTGTGGCCGGATGCCACCTTGAAGACCAAGTCAACCCCAAACGTTGCGGGCACTTGGACGGCA
AAGAAGTCGTGCGCACAGACGTGATGGTTCGACGCATCGCAGCCGCCGTCTCGGCCCGGCGCGACCCGAACTTTGTCATC
TGCGCCCGCACCGACGCCGCTGGAGTGGAAGGAATCGACGCCGCCATTGAGCGCGCGAAAGCCTACTTAGATGCGGGCGC
CGACATGATTTTCACCGAAGCCCTCCACAGCGAAGCCGACTTCCGATACTTCCGGCACGCCATCCCTGATGCCTTGTTGC
TGGCGAATATGACCGAATTTGGCAAAACGACGCTGCTGTCAGCCGACGTGTTGGAAGAGATTGGCTACAACGCCGTGATC
TACCCCGTGACCACGCTGCGTATTGCCATGGGACAAGTAGAACAAGCACTAGCCGAAATCAAAGAACACGGTACCCAAGA
AGGATGGCTGGACCGCATGCAACACCGCAGCAGATTATATGAGCTCCTGCGATACGAAGACTACAACGTCTTTGACCAGC
ACATTTTCACCTACAGAAAAGGAGAAAACAATGAGTGA

Upstream 100 bases:

>100_bases
TCTTGCATGCCGTGCAAAGCCTGCCTGACCTGGATGATCTTGATCAGCTCAACATCGAAGTCGACATAAGCAACCAGGCC
GCGACGAAAGCGGGGCTGTT

Downstream 100 bases:

>100_bases
CAGCCAAGTCCGCAAAGGACTCAACGGCGTCATCTCTGACTACACAAGCATTTCCAAAGTGATGCCAGAGAGCAACTCGC
TGACTTACCGTGGCTACGCC

Product: methylisocitric acid lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase 1

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MNLFSNGVDVGRRRQAFKAALAAPHIARLPGAFSPLIARSIEEAGFEGVYVSGAVIAADLALPDIGLTTLTEVAHRARQI
ARVTDLGVLVDADTGFGEPMSAARTVAELEDAGVAGCHLEDQVNPKRCGHLDGKEVVRTDVMVRRIAAAVSARRDPNFVI
CARTDAAGVEGIDAAIERAKAYLDAGADMIFTEALHSEADFRYFRHAIPDALLLANMTEFGKTTLLSADVLEEIGYNAVI
YPVTTLRIAMGQVEQALAEIKEHGTQEGWLDRMQHRSRLYELLRYEDYNVFDQHIFTYRKGENNE

Sequences:

>Translated_305_residues
MNLFSNGVDVGRRRQAFKAALAAPHIARLPGAFSPLIARSIEEAGFEGVYVSGAVIAADLALPDIGLTTLTEVAHRARQI
ARVTDLGVLVDADTGFGEPMSAARTVAELEDAGVAGCHLEDQVNPKRCGHLDGKEVVRTDVMVRRIAAAVSARRDPNFVI
CARTDAAGVEGIDAAIERAKAYLDAGADMIFTEALHSEADFRYFRHAIPDALLLANMTEFGKTTLLSADVLEEIGYNAVI
YPVTTLRIAMGQVEQALAEIKEHGTQEGWLDRMQHRSRLYELLRYEDYNVFDQHIFTYRKGENNE
>Mature_305_residues
MNLFSNGVDVGRRRQAFKAALAAPHIARLPGAFSPLIARSIEEAGFEGVYVSGAVIAADLALPDIGLTTLTEVAHRARQI
ARVTDLGVLVDADTGFGEPMSAARTVAELEDAGVAGCHLEDQVNPKRCGHLDGKEVVRTDVMVRRIAAAVSARRDPNFVI
CARTDAAGVEGIDAAIERAKAYLDAGADMIFTEALHSEADFRYFRHAIPDALLLANMTEFGKTTLLSADVLEEIGYNAVI
YPVTTLRIAMGQVEQALAEIKEHGTQEGWLDRMQHRSRLYELLRYEDYNVFDQHIFTYRKGENNE

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family

Homologues:

Organism=Escherichia coli, GI1786525, Length=290, Percent_Identity=43.448275862069, Blast_Score=217, Evalue=9e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRPB1_CORGL (Q8NSH8)

Other databases:

- EMBL:   AF434798
- EMBL:   BA000036
- EMBL:   BX927150
- RefSeq:   NP_599927.1
- RefSeq:   YP_224986.1
- ProteinModelPortal:   Q8NSH8
- SMR:   Q8NSH8
- GeneID:   1018694
- GeneID:   3343135
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg0797
- KEGG:   cgl:NCgl0665
- HOGENOM:   HBG728656
- OMA:   HSEADFR
- PhylomeDB:   Q8NSH8
- ProtClustDB:   CLSK632984
- BioCyc:   CGLU196627:CG0797-MONOMER
- BRENDA:   4.1.3.30
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- TIGRFAMs:   TIGR02317

Pfam domain/function: PF00463 ICL; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =4.1.3.30

Molecular weight: Translated: 33415; Mature: 33415

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLFSNGVDVGRRRQAFKAALAAPHIARLPGAFSPLIARSIEEAGFEGVYVSGAVIAADL
CCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHH
ALPDIGLTTLTEVAHRARQIARVTDLGVLVDADTGFGEPMSAARTVAELEDAGVAGCHLE
CCCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCC
DQVNPKRCGHLDGKEVVRTDVMVRRIAAAVSARRDPNFVICARTDAAGVEGIDAAIERAK
CCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHH
AYLDAGADMIFTEALHSEADFRYFRHAIPDALLLANMTEFGKTTLLSADVLEEIGYNAVI
HHHHCCCHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
YPVTTLRIAMGQVEQALAEIKEHGTQEGWLDRMQHRSRLYELLRYEDYNVFDQHIFTYRK
EHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
GENNE
CCCCC
>Mature Secondary Structure
MNLFSNGVDVGRRRQAFKAALAAPHIARLPGAFSPLIARSIEEAGFEGVYVSGAVIAADL
CCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHH
ALPDIGLTTLTEVAHRARQIARVTDLGVLVDADTGFGEPMSAARTVAELEDAGVAGCHLE
CCCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCC
DQVNPKRCGHLDGKEVVRTDVMVRRIAAAVSARRDPNFVICARTDAAGVEGIDAAIERAK
CCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHH
AYLDAGADMIFTEALHSEADFRYFRHAIPDALLLANMTEFGKTTLLSADVLEEIGYNAVI
HHHHCCCHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
YPVTTLRIAMGQVEQALAEIKEHGTQEGWLDRMQHRSRLYELLRYEDYNVFDQHIFTYRK
EHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
GENNE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11976302; 12948626