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The map label for this gene is exoA [H]

Identifier: 62389560

GI number: 62389560

Start: 687863

End: 688822

Strand: Direct

Name: exoA [H]

Synonym: cg0773

Alternate gene names: 62389560

Gene position: 687863-688822 (Clockwise)

Preceding gene: 62389559

Following gene: 62389561

Centisome position: 20.95

GC content: 55.83

Gene sequence:

>960_bases
TTGGCGGATGGAGGATTTCCCCAAGCACCCACTAGTGTGACAAGCATGAGTTTTCACATCACATCCGTCAATGTCAACGG
CATTAGGGCAGCGGTCAAACAGCGAAGCGAAACAAACCTAGGTTTCCTTCCGTGGCTTGAAGAAACTCGCCCGGACGTTG
TCCTCCTCCAAGAAGTCCGCGCAAGCGAAAAAGACACCGCCACCGCACTGCAACCCGCCTTAGATAACGGATGGCACTAC
ATTGGTGCCCCAGCAGCTGCCAAGGGACGTGCCGGTGTCGGCATTTTGTCTAGGCATGAACTTGAAGATGTGAACATCGG
TTTTGGATCTTTCCTTGACTCCGGCCGCTACATTGAAGCAACCATCAAAGACACCACCCTGGATGTGCCAGTAACCGTGG
CATCTCTTTACCTCCCCTCAGGTTCAGCGGGCACCGACAAGCAGGATGAAAAGTACCGCTTCCTCGATGAATTCGAAGGG
TTCCTGGACCAGCGCGCTAAAGAACGCTCCCACATGGTCATCGGTGGCGACTGGAACATCTGCCACCGCCGCGAAGACCT
GAAAAACTGGAAAACCAACCAAAAGAAATCCGGTTTCCTTCCCGACGAACGCGCATTCATGGATTCAGTCTTTGGCACCT
TCCCAGATGAGGCAACCCAGGTTGCAGGGGCCGGCGACTTCTTCGGTGCCGTGGACTATGAAGGAACGAGGCGTCGAGAA
GCAACTACGGACCCTGCGTGGTTCGACGTTGCACGTCGCCTGCAACCTGAAGGCGACGGCCCCTACACTTGGTGGACCTA
CCGCGGAAAAGCCTTCGACACCGGCGCCGGATGGCGCATCGACTACCAAGCAGCAACCGCAGCGATGCTCGAACGCGCAG
AACGCTCCTGGGTAGACAAAGCCGCTGCATACGATTTGCGCTGGTCAGATCACTCACCACTGAACGTGATCTACTCCTAA

Upstream 100 bases:

>100_bases
TTCCTACAGCACTCCAGCCCTTGCTGGCGCTGGCCTGGCATTCCTTGCCATCGTGGTGTGGTTCCCCATGGTGGCCTTGC
GTAAACGCACATAAACGGGG

Downstream 100 bases:

>100_bases
AATGCTGCTGACAATTCTATGGGCCATCGGCATCACCGCCGAAGGCATGACAGGCGCGCTGGCCGCAGGCCGACAAAAAA
TGGATCTCTTCGGAGTATCC

Product: exodeoxyribonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MADGGFPQAPTSVTSMSFHITSVNVNGIRAAVKQRSETNLGFLPWLEETRPDVVLLQEVRASEKDTATALQPALDNGWHY
IGAPAAAKGRAGVGILSRHELEDVNIGFGSFLDSGRYIEATIKDTTLDVPVTVASLYLPSGSAGTDKQDEKYRFLDEFEG
FLDQRAKERSHMVIGGDWNICHRREDLKNWKTNQKKSGFLPDERAFMDSVFGTFPDEATQVAGAGDFFGAVDYEGTRRRE
ATTDPAWFDVARRLQPEGDGPYTWWTYRGKAFDTGAGWRIDYQAATAAMLERAERSWVDKAAAYDLRWSDHSPLNVIYS

Sequences:

>Translated_319_residues
MADGGFPQAPTSVTSMSFHITSVNVNGIRAAVKQRSETNLGFLPWLEETRPDVVLLQEVRASEKDTATALQPALDNGWHY
IGAPAAAKGRAGVGILSRHELEDVNIGFGSFLDSGRYIEATIKDTTLDVPVTVASLYLPSGSAGTDKQDEKYRFLDEFEG
FLDQRAKERSHMVIGGDWNICHRREDLKNWKTNQKKSGFLPDERAFMDSVFGTFPDEATQVAGAGDFFGAVDYEGTRRRE
ATTDPAWFDVARRLQPEGDGPYTWWTYRGKAFDTGAGWRIDYQAATAAMLERAERSWVDKAAAYDLRWSDHSPLNVIYS
>Mature_318_residues
ADGGFPQAPTSVTSMSFHITSVNVNGIRAAVKQRSETNLGFLPWLEETRPDVVLLQEVRASEKDTATALQPALDNGWHYI
GAPAAAKGRAGVGILSRHELEDVNIGFGSFLDSGRYIEATIKDTTLDVPVTVASLYLPSGSAGTDKQDEKYRFLDEFEGF
LDQRAKERSHMVIGGDWNICHRREDLKNWKTNQKKSGFLPDERAFMDSVFGTFPDEATQVAGAGDFFGAVDYEGTRRREA
TTDPAWFDVARRLQPEGDGPYTWWTYRGKAFDTGAGWRIDYQAATAAMLERAERSWVDKAAAYDLRWSDHSPLNVIYS

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=302, Percent_Identity=29.4701986754967, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI18375503, Length=302, Percent_Identity=29.4701986754967, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI18375501, Length=302, Percent_Identity=29.4701986754967, Blast_Score=112, Evalue=6e-25,
Organism=Escherichia coli, GI1788046, Length=308, Percent_Identity=25.3246753246753, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 35534; Mature: 35402

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADGGFPQAPTSVTSMSFHITSVNVNGIRAAVKQRSETNLGFLPWLEETRPDVVLLQEVR
CCCCCCCCCCCCEEEEEEEEEEECCHHHHHHHHHHCCCCCCCCCCHHCCCCCEEEHHHHH
ASEKDTATALQPALDNGWHYIGAPAAAKGRAGVGILSRHELEDVNIGFGSFLDSGRYIEA
CCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEE
TIKDTTLDVPVTVASLYLPSGSAGTDKQDEKYRFLDEFEGFLDQRAKERSHMVIGGDWNI
EECCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCH
CHRREDLKNWKTNQKKSGFLPDERAFMDSVFGTFPDEATQVAGAGDFFGAVDYEGTRRRE
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCC
ATTDPAWFDVARRLQPEGDGPYTWWTYRGKAFDTGAGWRIDYQAATAAMLERAERSWVDK
CCCCHHHHHHHHHCCCCCCCCEEEEEECCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHH
AAAYDLRWSDHSPLNVIYS
HHHEEEECCCCCCCEEEEC
>Mature Secondary Structure 
ADGGFPQAPTSVTSMSFHITSVNVNGIRAAVKQRSETNLGFLPWLEETRPDVVLLQEVR
CCCCCCCCCCCEEEEEEEEEEECCHHHHHHHHHHCCCCCCCCCCHHCCCCCEEEHHHHH
ASEKDTATALQPALDNGWHYIGAPAAAKGRAGVGILSRHELEDVNIGFGSFLDSGRYIEA
CCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEE
TIKDTTLDVPVTVASLYLPSGSAGTDKQDEKYRFLDEFEGFLDQRAKERSHMVIGGDWNI
EECCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCH
CHRREDLKNWKTNQKKSGFLPDERAFMDSVFGTFPDEATQVAGAGDFFGAVDYEGTRRRE
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCC
ATTDPAWFDVARRLQPEGDGPYTWWTYRGKAFDTGAGWRIDYQAATAAMLERAERSWVDK
CCCCHHHHHHHHHCCCCCCCCEEEEEECCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHH
AAAYDLRWSDHSPLNVIYS
HHHEEEECCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]