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The map label for this gene is prpC2

Identifier: 62389549

GI number: 62389549

Start: 675081

End: 676232

Strand: Direct

Name: prpC2

Synonym: cg0762

Alternate gene names: 62389549

Gene position: 675081-676232 (Clockwise)

Preceding gene: 62389548

Following gene: 62389559

Centisome position: 20.56

GC content: 58.25

Gene sequence:

>1152_bases
ATGTCCAGCGCCACAACCACTGATGTTCGCAAAGGGCTCTACGGAGTCATCGCCGATTACACGGCCGTTTCCAAAGTCAT
GCCAGAGACCAATTCACTGACCTACCGTGGCTACGCGGTGGAAGATTTGGTGGAAAACTGCAGCTTCGAGGAGGTGTTTT
ACCTCCTGTGGCACGGCGAGCTGCCCACTGCGCAACAACTTGCGGAGTTCAATGAGCGTGGCCGTTCCTACCGCTCCCTG
GATGCCGGTTTGATCTCCCTGATCCACTCTTTGCCCAAAGAAGCCCACCCGATGGATGTTATGCGCACCGCGGTGTCCTA
CATGGGCACCAAGGATTCCGAGTATTTCACCACCGATTCTGAGCACATCCGCAAAGTTGGCCACACCTTGTTGGCGCAGC
TTCCGATGGTGCTAGCCATGGATATTCGTCGCCGCAAGGGCCTCGATATCATCGCCCCTGACTCCAGCAAGTCAGTCGCC
GAAAACCTGCTGTCTATGGTGTTTGGTACTGGCCCGGAATCACCTGCATCCAACCCAGCTGACGTCCGCGATTTTGAGAA
ATCACTGATCCTCTACGCCGAGCACTCCTTCAACGCCTCCACCTTCACCGCCCGCGTGATCACCTCCACCAAATCGGATG
TGTACTCCGCAATCACCGGCGCGATCGGTGCTCTCAAGGGCCCATTGCACGGTGGCGCCAACGAGTTTGTCATGCACACC
ATGTTGGCGATCGACGATCCCAACAAGGCCGCCGCCTGGATCAACAACGCTTTGGACAACAAGAATGTGGTCATGGGCTT
TGGCCACCGCGTGTACAAGCGCGGCGATTCCCGCGTGCCATCAATGGAGAAGTCCTTCCGGGAATTAGCTGCCCGCCACG
ACGGCGAAAAGTGGGTTGCCATGTATGAAAACATGCGCGACGCCATGGACGCCCGCACCGGCATCAAGCCGAATCTCGAT
TTCCCTGCTGGCCCTGCCTACCACCTGCTCGGTTTCCCGGTCGATTTCTTCACCCCGCTGTTCGTCATCGCCCGCGTCGC
CGGCTGGACGGCCCACATCGTGGAGCAGTACGAAAACAACTCGCTCATCCGCCCACTGTCCGAGTACAACGGCGAGGAGC
AGCGCGAGGTCGCGCCCATTGAAAAGCGCTAA

Upstream 100 bases:

>100_bases
TACAACGAGTACAACGCTTTCGACCAGCAAGTATTCACCTATTCCGCTGACAGCTACAAGCCCATCTTCTAACCCGCCTA
TATATAAGGAGTGAATCACC

Downstream 100 bases:

>100_bases
AAGATTTTCGCTTTTCGACGCCCCCTCCCACTCATTTCAGCCATGGTGCAACATTCTCGTCCCCACCATGTTCTTCTGAC
TCCCGGGAAGAACATGTTTC

Product: citrate synthase

Products: NA

Alternate protein names: Methylcitrate synthase 2; Citrate synthase 2

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MSSATTTDVRKGLYGVIADYTAVSKVMPETNSLTYRGYAVEDLVENCSFEEVFYLLWHGELPTAQQLAEFNERGRSYRSL
DAGLISLIHSLPKEAHPMDVMRTAVSYMGTKDSEYFTTDSEHIRKVGHTLLAQLPMVLAMDIRRRKGLDIIAPDSSKSVA
ENLLSMVFGTGPESPASNPADVRDFEKSLILYAEHSFNASTFTARVITSTKSDVYSAITGAIGALKGPLHGGANEFVMHT
MLAIDDPNKAAAWINNALDNKNVVMGFGHRVYKRGDSRVPSMEKSFRELAARHDGEKWVAMYENMRDAMDARTGIKPNLD
FPAGPAYHLLGFPVDFFTPLFVIARVAGWTAHIVEQYENNSLIRPLSEYNGEEQREVAPIEKR

Sequences:

>Translated_383_residues
MSSATTTDVRKGLYGVIADYTAVSKVMPETNSLTYRGYAVEDLVENCSFEEVFYLLWHGELPTAQQLAEFNERGRSYRSL
DAGLISLIHSLPKEAHPMDVMRTAVSYMGTKDSEYFTTDSEHIRKVGHTLLAQLPMVLAMDIRRRKGLDIIAPDSSKSVA
ENLLSMVFGTGPESPASNPADVRDFEKSLILYAEHSFNASTFTARVITSTKSDVYSAITGAIGALKGPLHGGANEFVMHT
MLAIDDPNKAAAWINNALDNKNVVMGFGHRVYKRGDSRVPSMEKSFRELAARHDGEKWVAMYENMRDAMDARTGIKPNLD
FPAGPAYHLLGFPVDFFTPLFVIARVAGWTAHIVEQYENNSLIRPLSEYNGEEQREVAPIEKR
>Mature_382_residues
SSATTTDVRKGLYGVIADYTAVSKVMPETNSLTYRGYAVEDLVENCSFEEVFYLLWHGELPTAQQLAEFNERGRSYRSLD
AGLISLIHSLPKEAHPMDVMRTAVSYMGTKDSEYFTTDSEHIRKVGHTLLAQLPMVLAMDIRRRKGLDIIAPDSSKSVAE
NLLSMVFGTGPESPASNPADVRDFEKSLILYAEHSFNASTFTARVITSTKSDVYSAITGAIGALKGPLHGGANEFVMHTM
LAIDDPNKAAAWINNALDNKNVVMGFGHRVYKRGDSRVPSMEKSFRELAARHDGEKWVAMYENMRDAMDARTGIKPNLDF
PAGPAYHLLGFPVDFFTPLFVIARVAGWTAHIVEQYENNSLIRPLSEYNGEEQREVAPIEKR

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also has a citrate synthase activity

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family

Homologues:

Organism=Homo sapiens, GI38327625, Length=384, Percent_Identity=23.9583333333333, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1786527, Length=372, Percent_Identity=40.0537634408602, Blast_Score=258, Evalue=5e-70,
Organism=Escherichia coli, GI1786939, Length=361, Percent_Identity=30.7479224376731, Blast_Score=172, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17555174, Length=371, Percent_Identity=23.7196765498652, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6319850, Length=412, Percent_Identity=25, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324328, Length=368, Percent_Identity=27.1739130434783, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24640126, Length=393, Percent_Identity=24.4274809160305, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24640124, Length=393, Percent_Identity=24.4274809160305, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRPC2_CORGL (Q8NSL1)

Other databases:

- EMBL:   AF434799
- EMBL:   BA000036
- EMBL:   BX927149
- RefSeq:   NP_599891.1
- RefSeq:   YP_224951.1
- ProteinModelPortal:   Q8NSL1
- SMR:   Q8NSL1
- GeneID:   1018659
- GeneID:   3342742
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg0762
- KEGG:   cgl:NCgl0630
- HOGENOM:   HBG649489
- OMA:   CASVIEY
- PhylomeDB:   Q8NSL1
- ProtClustDB:   PRK14033
- BioCyc:   CGLU196627:CG0762-MONOMER
- BRENDA:   2.3.3.5
- GO:   GO:0005737
- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- Gene3D:   G3DSA:1.10.580.10
- Gene3D:   G3DSA:1.10.230.10
- PANTHER:   PTHR11739
- PRINTS:   PR00143
- TIGRFAMs:   TIGR01800

Pfam domain/function: PF00285 Citrate_synt; SSF48256 Citrate_synthase_core

EC number: =2.3.3.5

Molecular weight: Translated: 42596; Mature: 42465

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: ACT_SITE 269-269 ACT_SITE 320-320

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSATTTDVRKGLYGVIADYTAVSKVMPETNSLTYRGYAVEDLVENCSFEEVFYLLWHGE
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHCCCHHHHEEHEECCC
LPTAQQLAEFNERGRSYRSLDAGLISLIHSLPKEAHPMDVMRTAVSYMGTKDSEYFTTDS
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEECCH
EHIRKVGHTLLAQLPMVLAMDIRRRKGLDIIAPDSSKSVAENLLSMVFGTGPESPASNPA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCC
DVRDFEKSLILYAEHSFNASTFTARVITSTKSDVYSAITGAIGALKGPLHGGANEFVMHT
HHHHHHHHEEEEEECCCCCHHHEEHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
MLAIDDPNKAAAWINNALDNKNVVMGFGHRVYKRGDSRVPSMEKSFRELAARHDGEKWVA
HHHCCCCCHHHHHHHHHCCCCCEEEECCHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHH
MYENMRDAMDARTGIKPNLDFPAGPAYHLLGFPVDFFTPLFVIARVAGWTAHIVEQYENN
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLIRPLSEYNGEEQREVAPIEKR
CCCCCHHHCCCCHHHHCCCCCCC
>Mature Secondary Structure 
SSATTTDVRKGLYGVIADYTAVSKVMPETNSLTYRGYAVEDLVENCSFEEVFYLLWHGE
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHCCCHHHHEEHEECCC
LPTAQQLAEFNERGRSYRSLDAGLISLIHSLPKEAHPMDVMRTAVSYMGTKDSEYFTTDS
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEECCH
EHIRKVGHTLLAQLPMVLAMDIRRRKGLDIIAPDSSKSVAENLLSMVFGTGPESPASNPA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCC
DVRDFEKSLILYAEHSFNASTFTARVITSTKSDVYSAITGAIGALKGPLHGGANEFVMHT
HHHHHHHHEEEEEECCCCCHHHEEHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
MLAIDDPNKAAAWINNALDNKNVVMGFGHRVYKRGDSRVPSMEKSFRELAARHDGEKWVA
HHHCCCCCHHHHHHHHHCCCCCEEEECCHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHH
MYENMRDAMDARTGIKPNLDFPAGPAYHLLGFPVDFFTPLFVIARVAGWTAHIVEQYENN
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLIRPLSEYNGEEQREVAPIEKR
CCCCCHHHCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11976302; 12948626