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The map label for this gene is prpB2

Identifier: 62389548

GI number: 62389548

Start: 674129

End: 675052

Strand: Direct

Name: prpB2

Synonym: cg0760

Alternate gene names: 62389548

Gene position: 674129-675052 (Clockwise)

Preceding gene: 62389547

Following gene: 62389549

Centisome position: 20.54

GC content: 59.96

Gene sequence:

>924_bases
ATGGCGGGTTTGTTTTCCTCTGCTGTTGCACCAACGGAGCGTCGAAAAGCATTACGCGCGGCACTGGCTGCGCCTGAAAT
TGCCCGCATGCCTGGTGCATTCTCCCCGCTGGCGGCGCGCGCAATCCAGGAAGCCGGATTTGAAGGCGTGTACGTCTCGG
GCGCCGTCGTGGCGGCTGACCTTGCATTGCCGGATATCGGCTTGACCACATTGACCGAAGTGGCGCACCGCTCCCGGCAG
ATCGCACGCGTGACAGACTTGCCCGTGCTGGTCGACGCCGACACCGGCTTCGGCGAACCCATGTCCGCAGCGCGCACCGT
CTCCGAACTCGAAGATGCAGGTGTCGCGGGCTGCCACCTGGAAGATCAAGTCAACCCCAAACGCTGTGGGCACCTGGACG
GAAAAGAAGTAGTGGGCACGGACATCATGGTTCGTCGCATCGCCGCAGCTGTCAACGAGCGTCGCGATGAGCAATTCGTC
ATCTGCGCTCGCACCGACGCCGCGGGAGTGGAAGGCATCGACTCCGCGATCGAGCGCGCCAAAGCTTACGCGGATGCCGG
CGCCGACATGATCTTCACCGAAGCGCTGTACAGCCCTGCAGATTTTGAAAAATTCCGCGCGGCCGTCGACATTCCGCTGC
TGGCCAACATGACGGAATTTGGCAAAACCGAACTTCTGCCCGCGCAGCTTCTGGAAGACATCGGATACAACGCAGTGATC
TACCCAGTGACCCTGCTGCGCATTGCGATGGGACAGGTCGAACAAGCTCTCGGCGACATTGCAAACACCGGAATCCAAAC
CGACTGGGTCGACCGGATGCAACACCGATCCAGGCTGTATGAGCTGCTGCGCTACAACGAGTACAACGCTTTCGACCAGC
AAGTATTCACCTATTCCGCTGACAGCTACAAGCCCATCTTCTAA

Upstream 100 bases:

>100_bases
CACAGCGTACGCACGAGCTTGAGGATCTTTCAGAACTCAACATTGAATTGGATGCCGATATTTTGGCCAAGGCTCCTGTG
ATTCCGGAAGGACTGTTCTG

Downstream 100 bases:

>100_bases
CCCGCCTATATATAAGGAGTGAATCACCATGTCCAGCGCCACAACCACTGATGTTCGCAAAGGGCTCTACGGAGTCATCG
CCGATTACACGGCCGTTTCC

Product: methylisocitric acid lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase 2

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MAGLFSSAVAPTERRKALRAALAAPEIARMPGAFSPLAARAIQEAGFEGVYVSGAVVAADLALPDIGLTTLTEVAHRSRQ
IARVTDLPVLVDADTGFGEPMSAARTVSELEDAGVAGCHLEDQVNPKRCGHLDGKEVVGTDIMVRRIAAAVNERRDEQFV
ICARTDAAGVEGIDSAIERAKAYADAGADMIFTEALYSPADFEKFRAAVDIPLLANMTEFGKTELLPAQLLEDIGYNAVI
YPVTLLRIAMGQVEQALGDIANTGIQTDWVDRMQHRSRLYELLRYNEYNAFDQQVFTYSADSYKPIF

Sequences:

>Translated_307_residues
MAGLFSSAVAPTERRKALRAALAAPEIARMPGAFSPLAARAIQEAGFEGVYVSGAVVAADLALPDIGLTTLTEVAHRSRQ
IARVTDLPVLVDADTGFGEPMSAARTVSELEDAGVAGCHLEDQVNPKRCGHLDGKEVVGTDIMVRRIAAAVNERRDEQFV
ICARTDAAGVEGIDSAIERAKAYADAGADMIFTEALYSPADFEKFRAAVDIPLLANMTEFGKTELLPAQLLEDIGYNAVI
YPVTLLRIAMGQVEQALGDIANTGIQTDWVDRMQHRSRLYELLRYNEYNAFDQQVFTYSADSYKPIF
>Mature_306_residues
AGLFSSAVAPTERRKALRAALAAPEIARMPGAFSPLAARAIQEAGFEGVYVSGAVVAADLALPDIGLTTLTEVAHRSRQI
ARVTDLPVLVDADTGFGEPMSAARTVSELEDAGVAGCHLEDQVNPKRCGHLDGKEVVGTDIMVRRIAAAVNERRDEQFVI
CARTDAAGVEGIDSAIERAKAYADAGADMIFTEALYSPADFEKFRAAVDIPLLANMTEFGKTELLPAQLLEDIGYNAVIY
PVTLLRIAMGQVEQALGDIANTGIQTDWVDRMQHRSRLYELLRYNEYNAFDQQVFTYSADSYKPIF

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family

Homologues:

Organism=Escherichia coli, GI1786525, Length=286, Percent_Identity=43.3566433566434, Blast_Score=217, Evalue=7e-58,
Organism=Escherichia coli, GI1790445, Length=215, Percent_Identity=29.3023255813954, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRPB2_CORGL (Q8NSL2)

Other databases:

- EMBL:   AF434799
- EMBL:   BA000036
- EMBL:   BX927149
- RefSeq:   NP_599890.2
- RefSeq:   YP_224950.1
- ProteinModelPortal:   Q8NSL2
- SMR:   Q8NSL2
- GeneID:   1018658
- GeneID:   3345588
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg0760
- KEGG:   cgl:NCgl0629
- HOGENOM:   HBG728656
- OMA:   TENVEAC
- PhylomeDB:   Q8NSL2
- ProtClustDB:   CLSK2484984
- BioCyc:   CGLU196627:CG0760-MONOMER
- BRENDA:   4.1.3.30
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- TIGRFAMs:   TIGR02317

Pfam domain/function: PF00463 ICL; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =4.1.3.30

Molecular weight: Translated: 33275; Mature: 33144

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGLFSSAVAPTERRKALRAALAAPEIARMPGAFSPLAARAIQEAGFEGVYVSGAVVAAD
CCCCHHHHCCCHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHCCCCCEEECCHHHHHH
LALPDIGLTTLTEVAHRSRQIARVTDLPVLVDADTGFGEPMSAARTVSELEDAGVAGCHL
HCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCC
EDQVNPKRCGHLDGKEVVGTDIMVRRIAAAVNERRDEQFVICARTDAAGVEGIDSAIERA
CCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHH
KAYADAGADMIFTEALYSPADFEKFRAAVDIPLLANMTEFGKTELLPAQLLEDIGYNAVI
HHHHHCCCHHHHHHHHCCCCCHHHHHHHHCCHHHHCHHHHCCCCCCHHHHHHHCCCCEEH
YPVTLLRIAMGQVEQALGDIANTGIQTDWVDRMQHRSRLYELLRYNEYNAFDQQVFTYSA
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEECC
DSYKPIF
CCCCCCC
>Mature Secondary Structure 
AGLFSSAVAPTERRKALRAALAAPEIARMPGAFSPLAARAIQEAGFEGVYVSGAVVAAD
CCCHHHHCCCHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHCCCCCEEECCHHHHHH
LALPDIGLTTLTEVAHRSRQIARVTDLPVLVDADTGFGEPMSAARTVSELEDAGVAGCHL
HCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCC
EDQVNPKRCGHLDGKEVVGTDIMVRRIAAAVNERRDEQFVICARTDAAGVEGIDSAIERA
CCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHH
KAYADAGADMIFTEALYSPADFEKFRAAVDIPLLANMTEFGKTELLPAQLLEDIGYNAVI
HHHHHCCCHHHHHHHHCCCCCHHHHHHHHCCHHHHCHHHHCCCCCCHHHHHHHCCCCEEH
YPVTLLRIAMGQVEQALGDIANTGIQTDWVDRMQHRSRLYELLRYNEYNAFDQQVFTYSA
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEECC
DSYKPIF
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11976302; 12948626