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The map label for this gene is spoU [H]

Identifier: 62389537

GI number: 62389537

Start: 662108

End: 662581

Strand: Reverse

Name: spoU [H]

Synonym: cg0749

Alternate gene names: 62389537

Gene position: 662581-662108 (Counterclockwise)

Preceding gene: 62389540

Following gene: 62389533

Centisome position: 20.18

GC content: 53.59

Gene sequence:

>474_bases
ATGCCGGAACACCCACTTCACGTTATCTTCGACAATCCTGTCATCCCTCCCAACACCGGAAACGCCATCCGAATGTGTGC
AGGAACAGGCGCTCACCTGCACCTTGTTGAACCTTTAGGCTTTGAGCTGACAGAAAAGCACCTTCGCCGAGCAGGCCTTG
ACTACCACGACTTAGCCGATGTCACAGTGCATGCAACCTTCGATGAAGCCATGGCTGCAGTCCCTGGTCGCGTATTTGCC
TTCACCACAACGGCCAATACCCGCTTCACCGATATCGCTTTTGAACCTGGCGATGCACTCCTTTTTGGAACTGAACCAAC
AGGACTCCCTCAAGAACATGTTGAGCATTCCCGAATCACCAGTGAGCTTCGGATCCCCATGCTTCCCGGTAGGCGTTCCA
TGAACCTTTCAAATTCCGCGGCGGTAGCGACCTATGAAGCATGGCGTCAACTCGGATTTGTGGGTGGGGTTTAG

Upstream 100 bases:

>100_bases
AGTCGGCGAAAATTTCCCCGCGGTATAAGTTTCCATCAAGTTTGATTGCAGTCACGCCCGCTATTATTCCACCCTTTTCC
AAGGCCTTACAATCAAACAC

Downstream 100 bases:

>100_bases
TTTTTTGCTGGGCTTCTGGGGCAGGCATGTGCCTCATTTAAAAAGCACCCCGGGGATAGATTCTCCGGGGTGCTTCTTTT
TTGATTGTGCTCGAGTAGTT

Product: tRNA/rRNA methyltransferase protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MPEHPLHVIFDNPVIPPNTGNAIRMCAGTGAHLHLVEPLGFELTEKHLRRAGLDYHDLADVTVHATFDEAMAAVPGRVFA
FTTTANTRFTDIAFEPGDALLFGTEPTGLPQEHVEHSRITSELRIPMLPGRRSMNLSNSAAVATYEAWRQLGFVGGV

Sequences:

>Translated_157_residues
MPEHPLHVIFDNPVIPPNTGNAIRMCAGTGAHLHLVEPLGFELTEKHLRRAGLDYHDLADVTVHATFDEAMAAVPGRVFA
FTTTANTRFTDIAFEPGDALLFGTEPTGLPQEHVEHSRITSELRIPMLPGRRSMNLSNSAAVATYEAWRQLGFVGGV
>Mature_156_residues
PEHPLHVIFDNPVIPPNTGNAIRMCAGTGAHLHLVEPLGFELTEKHLRRAGLDYHDLADVTVHATFDEAMAAVPGRVFAF
TTTANTRFTDIAFEPGDALLFGTEPTGLPQEHVEHSRITSELRIPMLPGRRSMNLSNSAAVATYEAWRQLGFVGGV

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=153, Percent_Identity=47.7124183006536, Blast_Score=152, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17139; Mature: 17007

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEHPLHVIFDNPVIPPNTGNAIRMCAGTGAHLHLVEPLGFELTEKHLRRAGLDYHDLAD
CCCCCEEEEECCCCCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHCCCCCHHCCC
VTVHATFDEAMAAVPGRVFAFTTTANTRFTDIAFEPGDALLFGTEPTGLPQEHVEHSRIT
EEEEEEHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCEEEECCCCCCCCHHHHHHHHHH
SELRIPMLPGRRSMNLSNSAAVATYEAWRQLGFVGGV
HHEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PEHPLHVIFDNPVIPPNTGNAIRMCAGTGAHLHLVEPLGFELTEKHLRRAGLDYHDLAD
CCCCEEEEECCCCCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHCCCCCHHCCC
VTVHATFDEAMAAVPGRVFAFTTTANTRFTDIAFEPGDALLFGTEPTGLPQEHVEHSRIT
EEEEEEHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCEEEECCCCCCCCHHHHHHHHHH
SELRIPMLPGRRSMNLSNSAAVATYEAWRQLGFVGGV
HHEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA