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The map label for this gene is menD [H]

Identifier: 62389365

GI number: 62389365

Start: 487027

End: 488658

Strand: Direct

Name: menD [H]

Synonym: cg0552

Alternate gene names: 62389365

Gene position: 487027-488658 (Clockwise)

Preceding gene: 62389364

Following gene: 62389366

Centisome position: 14.84

GC content: 57.05

Gene sequence:

>1632_bases
ATGTCCAGCACGCCAGCTCAAGATCTTGCCCGCGCCGTTATTGATTCCCTCGCACCACACGTCACTGACGTGGTGTTATG
CCCAGGATCCAGGAACTCACCGTTGTCGCTTGAGTTGCTGGCGCGGCAGGATCTGCGTGTCCATGTGCGTATCGACGAGC
GCAGCGCCTCATTTTTGGCGCTGTCCCTAGCGCGTACCCAGGCCCGGCCGGTGGCTGTGGTGATGACCTCCGGCACGGCT
GTAGCTAACTGCCTGCCTGCTGTTGCTGAAGCTGCGCATGCCCATATCCCGTTGATTGTGCTCTCTGCTGACCGTCCTGC
ACATTTGGTGGGAACGGGGGCGAGCCAAACGATTAACCAGACCGGTATTTTTGGTGATCTTGCACCGACGGTCGGTATCA
CTGAGCTGGATCAGGTAGCGCAGATTGCTGAAAGCCTTGCTCAGGGGGCTTCCCAGATTCCGCGTCATTTCAATCTTGCA
CTTGATGTTCCTTTGGTTGCTCCTGAACTGCCAGAGCTTCATGGTGAGGCAGTTGGAGCATCATGGACGCATCGCTGGAT
CAACCACGGTGAGGTGACCGTGGACCTGGGGGAGCACACCCTCGTGATTGCCGGTGATGAAGCATGGGAAGTGGAAGGGC
TGGAAGATGTGCCCACCATCGCTGAACCTACTGCACCAAAGCCTTATAATCCGGTGCACCCACTGGCTGCTGAAATCTTG
CTGAAGGAGCAGGTCTCCGCGGAAGGCTATGTGGTAAACACCAGGCCTGATCATGTGATCGTGGTGGGACACCCCACGCT
GCACCGCGGAGTGTTGAAGTTGATGTCAGATCCTGGCATTAAATTAACTGTGCTTTCACGCACCGATATCATCACTGATC
CCGGCCGCCATGCCGATCAGGTGGGCAGCACAGTGAAAGTCACCGGCACCCAGGAAAAGCAGTGGCTAAAGATCTGTTCG
GCAGCATCAGAACTTGCGGCCGATGGTGTGCGTGACGTCCTGGACAACCAAGAATTCGGTTTCACCGGCCTCCATGTTGC
CGCAGCCGTGGCGGATACCTTAGGCACCGGCGATACTCTCTTTGCTGCAGCATCCAACTCAATCCGTGACCTCTCCCTGG
TGGGTATGCCTTTTGATGGCGTGGATACCTTCTCCCCACGAGGTGTCGCAGGCATTGATGGTTCTGTTGCTCAAGCAATC
GGCACTTCACTTGCTGTGCAGTCCCGCCACCCCGATGAAATCCGCGCGCCACGCACTGTGGCCCTTCTGGGCGATCTGTC
GTTCCTTCACGATATTGGCGGACTGCTCATCGGCCCTGATGAACCACGCCCAGAAAACCTCACCATCGTGGTCTCCAACG
ACAACGGTGGCGGAATCTTCGAACTCCTAGAAACCGGCGCAGATGGTCTCCGCCCCAACTTCGAGCGTGCTTTCGGTACC
CCACACGACGCGTCCATCGCGGATCTCTGCGCAGGCTACGGCATTGAACACCAAGTGGTAGACAACCTCCAAGACCTCAT
CATCGCGCTAGTTGATACCACCGAAGTATCCGGATTCACCATTATTGAAGCTTCGACCGTCCGAGATACCCGCCGTGCAC
AACAGCAAGCTCTCATGGACACGGTGCACTAA

Upstream 100 bases:

>100_bases
CGTTTGGAAACGCTTGCTGCCAGCAAAGATAGGCGTGATTGGTGGTTTGAGCGCGTGCGTGAATCGTATCCGTACCTGGA
GACGATCTAGACTGTTGTGC

Downstream 100 bases:

>100_bases
ATGGAGTGGTACCAAGTGCGCCGACGGGCCCGACAACTGCTCATTGTCCTTTTTATAGCAGCGATGCTCGGAGCAGCCTC
CATGGTGATCGGGCCATTCC

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MSSTPAQDLARAVIDSLAPHVTDVVLCPGSRNSPLSLELLARQDLRVHVRIDERSASFLALSLARTQARPVAVVMTSGTA
VANCLPAVAEAAHAHIPLIVLSADRPAHLVGTGASQTINQTGIFGDLAPTVGITELDQVAQIAESLAQGASQIPRHFNLA
LDVPLVAPELPELHGEAVGASWTHRWINHGEVTVDLGEHTLVIAGDEAWEVEGLEDVPTIAEPTAPKPYNPVHPLAAEIL
LKEQVSAEGYVVNTRPDHVIVVGHPTLHRGVLKLMSDPGIKLTVLSRTDIITDPGRHADQVGSTVKVTGTQEKQWLKICS
AASELAADGVRDVLDNQEFGFTGLHVAAAVADTLGTGDTLFAAASNSIRDLSLVGMPFDGVDTFSPRGVAGIDGSVAQAI
GTSLAVQSRHPDEIRAPRTVALLGDLSFLHDIGGLLIGPDEPRPENLTIVVSNDNGGGIFELLETGADGLRPNFERAFGT
PHDASIADLCAGYGIEHQVVDNLQDLIIALVDTTEVSGFTIIEASTVRDTRRAQQQALMDTVH

Sequences:

>Translated_543_residues
MSSTPAQDLARAVIDSLAPHVTDVVLCPGSRNSPLSLELLARQDLRVHVRIDERSASFLALSLARTQARPVAVVMTSGTA
VANCLPAVAEAAHAHIPLIVLSADRPAHLVGTGASQTINQTGIFGDLAPTVGITELDQVAQIAESLAQGASQIPRHFNLA
LDVPLVAPELPELHGEAVGASWTHRWINHGEVTVDLGEHTLVIAGDEAWEVEGLEDVPTIAEPTAPKPYNPVHPLAAEIL
LKEQVSAEGYVVNTRPDHVIVVGHPTLHRGVLKLMSDPGIKLTVLSRTDIITDPGRHADQVGSTVKVTGTQEKQWLKICS
AASELAADGVRDVLDNQEFGFTGLHVAAAVADTLGTGDTLFAAASNSIRDLSLVGMPFDGVDTFSPRGVAGIDGSVAQAI
GTSLAVQSRHPDEIRAPRTVALLGDLSFLHDIGGLLIGPDEPRPENLTIVVSNDNGGGIFELLETGADGLRPNFERAFGT
PHDASIADLCAGYGIEHQVVDNLQDLIIALVDTTEVSGFTIIEASTVRDTRRAQQQALMDTVH
>Mature_542_residues
SSTPAQDLARAVIDSLAPHVTDVVLCPGSRNSPLSLELLARQDLRVHVRIDERSASFLALSLARTQARPVAVVMTSGTAV
ANCLPAVAEAAHAHIPLIVLSADRPAHLVGTGASQTINQTGIFGDLAPTVGITELDQVAQIAESLAQGASQIPRHFNLAL
DVPLVAPELPELHGEAVGASWTHRWINHGEVTVDLGEHTLVIAGDEAWEVEGLEDVPTIAEPTAPKPYNPVHPLAAEILL
KEQVSAEGYVVNTRPDHVIVVGHPTLHRGVLKLMSDPGIKLTVLSRTDIITDPGRHADQVGSTVKVTGTQEKQWLKICSA
ASELAADGVRDVLDNQEFGFTGLHVAAAVADTLGTGDTLFAAASNSIRDLSLVGMPFDGVDTFSPRGVAGIDGSVAQAIG
TSLAVQSRHPDEIRAPRTVALLGDLSFLHDIGGLLIGPDEPRPENLTIVVSNDNGGGIFELLETGADGLRPNFERAFGTP
HDASIADLCAGYGIEHQVVDNLQDLIIALVDTTEVSGFTIIEASTVRDTRRAQQQALMDTVH

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=116, Percent_Identity=43.9655172413793, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 57287; Mature: 57156

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTPAQDLARAVIDSLAPHVTDVVLCPGSRNSPLSLELLARQDLRVHVRIDERSASFLA
CCCCCHHHHHHHHHHHHCCHHEEEEECCCCCCCCEEEEEEECCCCEEEEEECCCCCCEEE
LSLARTQARPVAVVMTSGTAVANCLPAVAEAAHAHIPLIVLSADRPAHLVGTGASQTINQ
EEHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCHHHHC
TGIFGDLAPTVGITELDQVAQIAESLAQGASQIPRHFNLALDVPLVAPELPELHGEAVGA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHCCCCCCC
SWTHRWINHGEVTVDLGEHTLVIAGDEAWEVEGLEDVPTIAEPTAPKPYNPVHPLAAEIL
CHHHHHCCCCEEEEEECCEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LKEQVSAEGYVVNTRPDHVIVVGHPTLHRGVLKLMSDPGIKLTVLSRTDIITDPGRHADQ
HHHHCCCCCEEEECCCCEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHH
VGSTVKVTGTQEKQWLKICSAASELAADGVRDVLDNQEFGFTGLHVAAAVADTLGTGDTL
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEE
FAAASNSIRDLSLVGMPFDGVDTFSPRGVAGIDGSVAQAIGTSLAVQSRHPDEIRAPRTV
EEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEECCCCCHHHCCCEEE
ALLGDLSFLHDIGGLLIGPDEPRPENLTIVVSNDNGGGIFELLETGADGLRPNFERAFGT
EEHHHHHHHHHCCCEEECCCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCCCCHHHHCCC
PHDASIADLCAGYGIEHQVVDNLQDLIIALVDTTEVSGFTIIEASTVRDTRRAQQQALMD
CCCCCHHHHHHCCCCCHHHHHHHHHHHHHHEECCCCCCEEEEEEHHHHHHHHHHHHHHHH
TVH
HCC
>Mature Secondary Structure 
SSTPAQDLARAVIDSLAPHVTDVVLCPGSRNSPLSLELLARQDLRVHVRIDERSASFLA
CCCCHHHHHHHHHHHHCCHHEEEEECCCCCCCCEEEEEEECCCCEEEEEECCCCCCEEE
LSLARTQARPVAVVMTSGTAVANCLPAVAEAAHAHIPLIVLSADRPAHLVGTGASQTINQ
EEHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCHHHHC
TGIFGDLAPTVGITELDQVAQIAESLAQGASQIPRHFNLALDVPLVAPELPELHGEAVGA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHCCCCCCC
SWTHRWINHGEVTVDLGEHTLVIAGDEAWEVEGLEDVPTIAEPTAPKPYNPVHPLAAEIL
CHHHHHCCCCEEEEEECCEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LKEQVSAEGYVVNTRPDHVIVVGHPTLHRGVLKLMSDPGIKLTVLSRTDIITDPGRHADQ
HHHHCCCCCEEEECCCCEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHH
VGSTVKVTGTQEKQWLKICSAASELAADGVRDVLDNQEFGFTGLHVAAAVADTLGTGDTL
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEE
FAAASNSIRDLSLVGMPFDGVDTFSPRGVAGIDGSVAQAIGTSLAVQSRHPDEIRAPRTV
EEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEECCCCCHHHCCCEEE
ALLGDLSFLHDIGGLLIGPDEPRPENLTIVVSNDNGGGIFELLETGADGLRPNFERAFGT
EEHHHHHHHHHCCCEEECCCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCCCCHHHHCCC
PHDASIADLCAGYGIEHQVVDNLQDLIIALVDTTEVSGFTIIEASTVRDTRRAQQQALMD
CCCCCHHHHHHCCCCCHHHHHHHHHHHHHHEECCCCCCEEEEEEHHHHHHHHHHHHHHHH
TVH
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA