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The map label for this gene is shiA [H]

Identifier: 62389321

GI number: 62389321

Start: 442757

End: 444160

Strand: Direct

Name: shiA [H]

Synonym: cg0501

Alternate gene names: 62389321

Gene position: 442757-444160 (Clockwise)

Preceding gene: 62389319

Following gene: 62389322

Centisome position: 13.49

GC content: 54.42

Gene sequence:

>1404_bases
ATGAGCGAACAACTTCAGGGTGTAACTCACTCCGAATCAACTCCGGGCAAGACGCCCAAGCGAGCAGCACTATCCAGCTG
GATCGGCTCAGCTCTCGAATACTACGACTTCGCTGTTTACGGAACCGCTGCAGCGCTGGTTCTTAACCACCTCTTCTTCC
CAGCTGATACTTCACCAGGCATCGCAATTTTGGCTGCGATGGGTACCGTGGGTGTTGCTTATGTGGTTCGCCCTCTTGGT
GCGCTGATCATGGGTCCATTAGGTGACCGTTACGGACGTAAATTTGTCCTCATGCTGTGCCTCTTCCTGATTGGAGCATC
CACTTTCGCAGTTGGCTGCTTGCCAACATTTGATCAGGTCGGTTACTTGGCTCCGGCACTGTTGGTGCTGTGCCGTGTGA
TCCAGGGACTGTCTGCATCCGGTGAGCAGTCCAGTGCGATTTCCGTTTCTTTGGAGCACGCCGATGAGCGTCACCGCGCA
TTTACTGCTAGCTGGACTCTTCACGGAACCCAGTTCGGTACCTTGCTGGCAACCGGAGTATTTATCCCATTCACCTTGTT
CCTGAGTGAAGATGCTCTAATGTCATGGGGTTGGCGCGTTCCGTTCTGGCTGTCCGCTGCTGTTGTTTTGGTTGCTTTCC
TCATCCGTCGTGGACTGGAAGAGCCACCAGCATTCCGTGAAAACAAGGAAGCAGTTGCAGGCGCAGCATCTCCACTGGCG
ATGACCTTGCGTTACCACAAGGCGGCGGTTGCTCGCGTTGCTATTGCTGCGATGATCAACTCCGTGAACATTGTGTTTAC
TGTGTGGGCACTGTCGTTCGCCACCAACATTGTTGGCCTGGATCGTTCAACTGTTTTGCTGGTTCCAGTTGTTGCGAACT
TGGTTGCACTGATTGCGATTCCTTTGTCCGGCATGCTGGCTGACCGCATTGGTCGCCGACCAGTGTTCATCATGGGTGCC
ATTGGTGGTGGCCTGGCCATGAACGGTTACCTGGGAGCTATCTACTCCGGCAATTGGACCATGATCTTCTTCATGGGCGT
GTTGATGTCTGGTCTGCTGTACTCCATGGGTAATGCCGTGTGGCCAGCGTTCTACGCAGAAATGTTCCCAACCTCTGTGC
GTGTCACCGGCTTGGCTCTTGGAACTCAGATTGGTTTCGCAGTCTCTGGTGGTTTCGTCCCAGTTATCGCATCCGCGCTT
GCTGGTGATCAGGGTGACCAGTGGATGAAGGTGTCCATCTTCGTTGGTGTTGTTTGTGTGATTTCTGCACTGGTTGCCAT
GACCGCTAAGGAAACCAAGGCTCTGACTCTGGATGAGATCGATGCTCTGCACACTGCTGGTGGTGAGGCCGCAGACCTGG
CAGCCGCAAGCAAAGCCTCCGAGGCCCAACTCGCGGCTCAGTAA

Upstream 100 bases:

>100_bases
ATTACCCACAATTTCAATCGGTTTATACAACCAGCCTCTAACTGGCAACAGGACTGCAGACAGAAACTGTTGCTGGAACC
TTCGATGAACAGGATCGACA

Downstream 100 bases:

>100_bases
AACCAAAAGGAATCTTTGACCACATGCGTACATCCATTGCCACTGTTTGTTTGTCCGGAACTCTTGCTGAAAAGCTGCGC
GCAGCTGCAGATGCTGGATT

Product: integral membrane transport protein

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSEQLQGVTHSESTPGKTPKRAALSSWIGSALEYYDFAVYGTAAALVLNHLFFPADTSPGIAILAAMGTVGVAYVVRPLG
ALIMGPLGDRYGRKFVLMLCLFLIGASTFAVGCLPTFDQVGYLAPALLVLCRVIQGLSASGEQSSAISVSLEHADERHRA
FTASWTLHGTQFGTLLATGVFIPFTLFLSEDALMSWGWRVPFWLSAAVVLVAFLIRRGLEEPPAFRENKEAVAGAASPLA
MTLRYHKAAVARVAIAAMINSVNIVFTVWALSFATNIVGLDRSTVLLVPVVANLVALIAIPLSGMLADRIGRRPVFIMGA
IGGGLAMNGYLGAIYSGNWTMIFFMGVLMSGLLYSMGNAVWPAFYAEMFPTSVRVTGLALGTQIGFAVSGGFVPVIASAL
AGDQGDQWMKVSIFVGVVCVISALVAMTAKETKALTLDEIDALHTAGGEAADLAAASKASEAQLAAQ

Sequences:

>Translated_467_residues
MSEQLQGVTHSESTPGKTPKRAALSSWIGSALEYYDFAVYGTAAALVLNHLFFPADTSPGIAILAAMGTVGVAYVVRPLG
ALIMGPLGDRYGRKFVLMLCLFLIGASTFAVGCLPTFDQVGYLAPALLVLCRVIQGLSASGEQSSAISVSLEHADERHRA
FTASWTLHGTQFGTLLATGVFIPFTLFLSEDALMSWGWRVPFWLSAAVVLVAFLIRRGLEEPPAFRENKEAVAGAASPLA
MTLRYHKAAVARVAIAAMINSVNIVFTVWALSFATNIVGLDRSTVLLVPVVANLVALIAIPLSGMLADRIGRRPVFIMGA
IGGGLAMNGYLGAIYSGNWTMIFFMGVLMSGLLYSMGNAVWPAFYAEMFPTSVRVTGLALGTQIGFAVSGGFVPVIASAL
AGDQGDQWMKVSIFVGVVCVISALVAMTAKETKALTLDEIDALHTAGGEAADLAAASKASEAQLAAQ
>Mature_466_residues
SEQLQGVTHSESTPGKTPKRAALSSWIGSALEYYDFAVYGTAAALVLNHLFFPADTSPGIAILAAMGTVGVAYVVRPLGA
LIMGPLGDRYGRKFVLMLCLFLIGASTFAVGCLPTFDQVGYLAPALLVLCRVIQGLSASGEQSSAISVSLEHADERHRAF
TASWTLHGTQFGTLLATGVFIPFTLFLSEDALMSWGWRVPFWLSAAVVLVAFLIRRGLEEPPAFRENKEAVAGAASPLAM
TLRYHKAAVARVAIAAMINSVNIVFTVWALSFATNIVGLDRSTVLLVPVVANLVALIAIPLSGMLADRIGRRPVFIMGAI
GGGLAMNGYLGAIYSGNWTMIFFMGVLMSGLLYSMGNAVWPAFYAEMFPTSVRVTGLALGTQIGFAVSGGFVPVIASALA
GDQGDQWMKVSIFVGVVCVISALVAMTAKETKALTLDEIDALHTAGGEAADLAAASKASEAQLAAQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=417, Percent_Identity=35.4916067146283, Blast_Score=254, Evalue=8e-69,
Organism=Escherichia coli, GI1790550, Length=424, Percent_Identity=28.3018867924528, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1789941, Length=411, Percent_Identity=33.5766423357664, Blast_Score=188, Evalue=8e-49,
Organism=Escherichia coli, GI1788942, Length=392, Percent_Identity=28.8265306122449, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI87082404, Length=420, Percent_Identity=23.8095238095238, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082231, Length=230, Percent_Identity=23.9130434782609, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49111; Mature: 48980

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQLQGVTHSESTPGKTPKRAALSSWIGSALEYYDFAVYGTAAALVLNHLFFPADTSPG
CCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IAILAAMGTVGVAYVVRPLGALIMGPLGDRYGRKFVLMLCLFLIGASTFAVGCLPTFDQV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHH
GYLAPALLVLCRVIQGLSASGEQSSAISVSLEHADERHRAFTASWTLHGTQFGTLLATGV
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHCHHHHHEEEEEEEECCCHHHHHHHHHH
FIPFTLFLSEDALMSWGWRVPFWLSAAVVLVAFLIRRGLEEPPAFRENKEAVAGAASPLA
HHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCHHH
MTLRYHKAAVARVAIAAMINSVNIVFTVWALSFATNIVGLDRSTVLLVPVVANLVALIAI
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHH
PLSGMLADRIGRRPVFIMGAIGGGLAMNGYLGAIYSGNWTMIFFMGVLMSGLLYSMGNAV
HHHHHHHHHHCCCCEEEEEECCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHCCHH
WPAFYAEMFPTSVRVTGLALGTQIGFAVSGGFVPVIASALAGDQGDQWMKVSIFVGVVCV
HHHHHHHHCCCCEEEEEEEHHHHHCCEECCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHH
ISALVAMTAKETKALTLDEIDALHTAGGEAADLAAASKASEAQLAAQ
HHHHHHHHHHHHCCEEHHHHHHHHCCCCCHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure 
SEQLQGVTHSESTPGKTPKRAALSSWIGSALEYYDFAVYGTAAALVLNHLFFPADTSPG
CCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IAILAAMGTVGVAYVVRPLGALIMGPLGDRYGRKFVLMLCLFLIGASTFAVGCLPTFDQV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHH
GYLAPALLVLCRVIQGLSASGEQSSAISVSLEHADERHRAFTASWTLHGTQFGTLLATGV
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHCHHHHHEEEEEEEECCCHHHHHHHHHH
FIPFTLFLSEDALMSWGWRVPFWLSAAVVLVAFLIRRGLEEPPAFRENKEAVAGAASPLA
HHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCHHH
MTLRYHKAAVARVAIAAMINSVNIVFTVWALSFATNIVGLDRSTVLLVPVVANLVALIAI
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHH
PLSGMLADRIGRRPVFIMGAIGGGLAMNGYLGAIYSGNWTMIFFMGVLMSGLLYSMGNAV
HHHHHHHHHHCCCCEEEEEECCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHCCHH
WPAFYAEMFPTSVRVTGLALGTQIGFAVSGGFVPVIASALAGDQGDQWMKVSIFVGVVCV
HHHHHHHHCCCCEEEEEEEHHHHHCCEECCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHH
ISALVAMTAKETKALTLDEIDALHTAGGEAADLAAASKASEAQLAAQ
HHHHHHHHHHHHCCEEHHHHHHHHCCCCCHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]