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The map label for this gene is gltX

Identifier: 62389134

GI number: 62389134

Start: 251953

End: 252834

Strand: Direct

Name: gltX

Synonym: cg0289

Alternate gene names: 62389134

Gene position: 251953-252834 (Clockwise)

Preceding gene: 62389133

Following gene: 62389138

Centisome position: 7.68

GC content: 60.09

Gene sequence:

>882_bases
ATGGCAGGACGATACGCACCATCACCAAGCGGCGACCTTCACTTTGGCAACCTCCGCACAGCACTGCTGGCCTGGCTGTT
CGCGCGCTCCGAAGGAAAAAAATTCCTCATGCGGGTCGAAGACATCGATGAACAACGCTCATCCAAGGAATCCGCCGAAA
GCCAACTCGCAGACCTATCCGCCCTGGGTCTCGATTGGGATGGCGACGTCCTCTACCAATCCACACGCTACGACGCCTAC
CGCGCAGCCCTTGAAAAACTAGACACCTACGAATGTTATTGCTCGCGCCGGGACATCCAAGAAGCCTCGCGGGCACCCCA
TGTGGCTCCGGGAGTGTATCCGGGAACGTGTAGGGGATTGAAGGAGGAGGAACGCGTCGAAAAGCGTGCAACCTTGGCTG
CGCAAAACCGGCACCCCGCCATCCGCCTGCGCGCGCAGGTAACCTCGTTTGATTTTCACGACCGACTTCGCGGCCCACAA
ACTGGCCCCGTAGACGATTTCATTCTGCTCCGCGGCGGGCAGGAACCCGGATGGGCATACAACTTAGCTGTCGTCGTCGA
CGATGCCTACCAAGGCGTTGACCAGGTAGTCCGCGGCGACGACCTACTCGATTCCGCCGCGCGCCAAGCCTACCTCGGCT
CGCTGCTGGGCACCCCCGCGCCCGAATACATTCACGTGCCGCTCGTGCTCAACGCCCACGGCCAGCGCCTCGCCAAACGC
GACGGGGCAGTGACGCTTAAAGAAATGCTTATCGACGCCCCCCTCCACACCATTTTCTCCCGCCTCGCATCATCGCTCGG
CTACGAAGGGGTAAATTCCGCACCCCAATTGTTGGAAATTTTCGACCCCACAACCCTCAGCCGGGAGCCGTTTATTTACT
GA

Upstream 100 bases:

>100_bases
CACCCCTGAAAACCTCCTCAACTATCCCGGAGTGATCATCTCCACCGTTCAGGAGAACCCATCCGAAACATGGCGGCAAG
TGAACATCTAATCTAGAAAC

Downstream 100 bases:

>100_bases
GGCTCAGAGGGAGGGGTCATTCCATCTGGAGGAGTCATGCCGCCTGGTCCTCCTTGTCCACCGGGGCCTCCCTGGCCTCC
ACTTGGCATGTTCATGGACT

Product: glutamyl-Q tRNA(Asp) synthetase

Products: AMP; diphosphate; L-glutamyl-tRNA(Glu)

Alternate protein names: Glu-Q-RSs

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MAGRYAPSPSGDLHFGNLRTALLAWLFARSEGKKFLMRVEDIDEQRSSKESAESQLADLSALGLDWDGDVLYQSTRYDAY
RAALEKLDTYECYCSRRDIQEASRAPHVAPGVYPGTCRGLKEEERVEKRATLAAQNRHPAIRLRAQVTSFDFHDRLRGPQ
TGPVDDFILLRGGQEPGWAYNLAVVVDDAYQGVDQVVRGDDLLDSAARQAYLGSLLGTPAPEYIHVPLVLNAHGQRLAKR
DGAVTLKEMLIDAPLHTIFSRLASSLGYEGVNSAPQLLEIFDPTTLSREPFIY

Sequences:

>Translated_293_residues
MAGRYAPSPSGDLHFGNLRTALLAWLFARSEGKKFLMRVEDIDEQRSSKESAESQLADLSALGLDWDGDVLYQSTRYDAY
RAALEKLDTYECYCSRRDIQEASRAPHVAPGVYPGTCRGLKEEERVEKRATLAAQNRHPAIRLRAQVTSFDFHDRLRGPQ
TGPVDDFILLRGGQEPGWAYNLAVVVDDAYQGVDQVVRGDDLLDSAARQAYLGSLLGTPAPEYIHVPLVLNAHGQRLAKR
DGAVTLKEMLIDAPLHTIFSRLASSLGYEGVNSAPQLLEIFDPTTLSREPFIY
>Mature_292_residues
AGRYAPSPSGDLHFGNLRTALLAWLFARSEGKKFLMRVEDIDEQRSSKESAESQLADLSALGLDWDGDVLYQSTRYDAYR
AALEKLDTYECYCSRRDIQEASRAPHVAPGVYPGTCRGLKEEERVEKRATLAAQNRHPAIRLRAQVTSFDFHDRLRGPQT
GPVDDFILLRGGQEPGWAYNLAVVVDDAYQGVDQVVRGDDLLDSAARQAYLGSLLGTPAPEYIHVPLVLNAHGQRLAKRD
GAVTLKEMLIDAPLHTIFSRLASSLGYEGVNSAPQLLEIFDPTTLSREPFIY

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily

Homologues:

Organism=Homo sapiens, GI134288884, Length=314, Percent_Identity=31.5286624203822, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI226510925, Length=254, Percent_Identity=42.5196850393701, Blast_Score=177, Evalue=9e-46,
Organism=Escherichia coli, GI1788743, Length=302, Percent_Identity=33.112582781457, Blast_Score=163, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI71993394, Length=254, Percent_Identity=29.1338582677165, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324540, Length=265, Percent_Identity=32.4528301886792, Blast_Score=125, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24665321, Length=266, Percent_Identity=32.7067669172932, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLUQ_CORGL (Q8NTR6)

Other databases:

- EMBL:   BA000036
- EMBL:   BX927148
- RefSeq:   NP_599489.1
- RefSeq:   YP_224536.1
- ProteinModelPortal:   Q8NTR6
- SMR:   Q8NTR6
- GeneID:   1021302
- GeneID:   3344120
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg0289
- KEGG:   cgl:NCgl0233
- HOGENOM:   HBG628189
- OMA:   IIKRSDG
- PhylomeDB:   Q8NTR6
- ProtClustDB:   PRK05710
- BioCyc:   CGLU196627:CG0289-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01428
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR03838

Pfam domain/function: PF00749 tRNA-synt_1c

EC number: 6.1.1.17

Molecular weight: Translated: 32552; Mature: 32421

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 40-40 BINDING 180-180 BINDING 198-198 BINDING 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRYAPSPSGDLHFGNLRTALLAWLFARSEGKKFLMRVEDIDEQRSSKESAESQLADLS
CCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGLDWDGDVLYQSTRYDAYRAALEKLDTYECYCSRRDIQEASRAPHVAPGVYPGTCRGL
HHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCC
KEEERVEKRATLAAQNRHPAIRLRAQVTSFDFHDRLRGPQTGPVDDFILLRGGQEPGWAY
CHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHCCCCCCCCCCEEEEECCCCCCCEE
NLAVVVDDAYQGVDQVVRGDDLLDSAARQAYLGSLLGTPAPEYIHVPLVLNAHGQRLAKR
EEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHC
DGAVTLKEMLIDAPLHTIFSRLASSLGYEGVNSAPQLLEIFDPTTLSREPFIY
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AGRYAPSPSGDLHFGNLRTALLAWLFARSEGKKFLMRVEDIDEQRSSKESAESQLADLS
CCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGLDWDGDVLYQSTRYDAYRAALEKLDTYECYCSRRDIQEASRAPHVAPGVYPGTCRGL
HHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCC
KEEERVEKRATLAAQNRHPAIRLRAQVTSFDFHDRLRGPQTGPVDDFILLRGGQEPGWAY
CHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHCCCCCCCCCCEEEEECCCCCCCEE
NLAVVVDDAYQGVDQVVRGDDLLDSAARQAYLGSLLGTPAPEYIHVPLVLNAHGQRLAKR
EEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHC
DGAVTLKEMLIDAPLHTIFSRLASSLGYEGVNSAPQLLEIFDPTTLSREPFIY
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; tRNA(Glu)

Specific reaction: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626