Click here to switch to the map view.

The map label for this gene is gltD [H]

Identifier: 62389080

GI number: 62389080

Start: 199773

End: 201293

Strand: Direct

Name: gltD [H]

Synonym: cg0230

Alternate gene names: 62389080

Gene position: 199773-201293 (Clockwise)

Preceding gene: 62389079

Following gene: 62389089

Centisome position: 6.09

GC content: 60.68

Gene sequence:

>1521_bases
ATGGCCGACCCACAAGGATTCATCAAATACTCCCGACGCGAGCCTGCACACCGCCCGGTCCCGCTGCGCCTCATGGACCA
CTCCGAGGTCTACGAAAAGGCACCGGCAGGTCAGATCGAGGAACAGGCTGCCCGCTGCATGGATTGCGGTGTCCCGTTCT
GCCACGAAGGCTGCCCACTGGGCAACATCATCCCTGAGTGGAATGATCTGGTACGCCAAGGTCGGTGGAAGGAAGCCTAC
GATCGCTTGCACGCGACCAACAATTTCCCCGAGTTCACCGGCCGTTTGTGCCCCGCACCCTGCGAAGGCGCCTGCGTGCT
CGGTATCAACGATGATTCTGTCACCATCAAAAACGTTGAGCTGGAAATCGTCGAAAAAGCATTCCGCGAAGGCTGGGTGC
AACCAGTAATCCCATCCATGTCTACCGGGCTGTCAGTCGCCGTCGTCGGTTCCGGCCCCGCTGGCCTTGCCGCCGCGCAG
CAGCTCACCCGCGCAGGCCACAGCGTTACCGTCTTTGAACGCGACGACCGCCTCGGCGGCCTCATGCGCTACGGCGTGCC
AGAATACAAAATGGAGAACCGCTGGATCGACCGCCGCATCGAGCAAATGGAAGCAGAGGGCACAACTTTCCAGGTAGGCA
CCTCGCCGCGCGCCGCTGAACTAGCGCTTTTCGACGCGATCCTCCTCGCAACCGGCACCCCAGTGGCCCGCGAACTCTCA
GTTCCAGGCCACGATCTCAACGGCATCCATGCGGCAATGGATTACCTCACCGCCCAAAACCGCATCAACGAAGGCGACGG
TGAAGTCTCTCCAATCAACGCCAAAGGCAAGAAAGTTGTCATCATCGGTGGCGGCGACACCGGCACCGACTGCTTCGGCA
CCGCACTGCGCCAAGGAGCAGAATCAGTCACCCAATTTGATATCCGCCCCCGCGCTCCTTTCCAGCGCGCCGATTCCACC
CCATGGCCGATGTACCCCAACCTCTTCCGCACCGCAACGGCTCACGAAGAAGGCGAATACATCATCACTGGCGATGAATC
AGCCGATGAAATCGCAGCCCTGGGCCTCGCCGAACGTGCCGCAGGCTCCACGCTTGGTGAACGTAAATTTGCTGTCAACA
CCGTGGAATTCCACGGCAACAACGGCCACGTCACCGGACTCACCGGCAACCAAATCCGAGTTGTCAACGGCAAACGTGAA
CCAATCGAAGGCACCGAATTCCCCTTCGAAGCAGACCTCGTTCTTGTTGCACTTGGCTTCACCGGCGCAGAACAAGGCGG
ATTGGCACACGAACTAGGCGTAGGTTTCGACGACCGGGGCCGCATCCTCCGCGATTCCGAATACCGCAGCCCCACCAACT
CCCGCGTTTACATCGCAGGCGACAACGGCCGTGGCCAGTCCCTGATCGTGTGGGCAATCGCCGAAGGCCGCGCATGCGCC
GCAGCTATCGACGCCGATCTCATGGGTGAAACTGCACTCCCTGTAGCAGTTGCACCACAGGACGTGCCGCTGGCTGTCTA
G

Upstream 100 bases:

>100_bases
AAATCATGCCGCGCGATTTCCAAAAAGTACTCAACATCATCGAAACGGCCCACGCTGAGGGCCAAGACCCAGCAATCAAG
ATCATGGAGGCAGTGAGCTA

Downstream 100 bases:

>100_bases
CTTGGGTAGAAAATGCTAGAAACCACCATCAGTAATTGGTGGTTTCTAGCGTTTTACGGGATGCATCATCGCATAGACAG
CACTGGCTAGTCCTAGCAGG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MADPQGFIKYSRREPAHRPVPLRLMDHSEVYEKAPAGQIEEQAARCMDCGVPFCHEGCPLGNIIPEWNDLVRQGRWKEAY
DRLHATNNFPEFTGRLCPAPCEGACVLGINDDSVTIKNVELEIVEKAFREGWVQPVIPSMSTGLSVAVVGSGPAGLAAAQ
QLTRAGHSVTVFERDDRLGGLMRYGVPEYKMENRWIDRRIEQMEAEGTTFQVGTSPRAAELALFDAILLATGTPVARELS
VPGHDLNGIHAAMDYLTAQNRINEGDGEVSPINAKGKKVVIIGGGDTGTDCFGTALRQGAESVTQFDIRPRAPFQRADST
PWPMYPNLFRTATAHEEGEYIITGDESADEIAALGLAERAAGSTLGERKFAVNTVEFHGNNGHVTGLTGNQIRVVNGKRE
PIEGTEFPFEADLVLVALGFTGAEQGGLAHELGVGFDDRGRILRDSEYRSPTNSRVYIAGDNGRGQSLIVWAIAEGRACA
AAIDADLMGETALPVAVAPQDVPLAV

Sequences:

>Translated_506_residues
MADPQGFIKYSRREPAHRPVPLRLMDHSEVYEKAPAGQIEEQAARCMDCGVPFCHEGCPLGNIIPEWNDLVRQGRWKEAY
DRLHATNNFPEFTGRLCPAPCEGACVLGINDDSVTIKNVELEIVEKAFREGWVQPVIPSMSTGLSVAVVGSGPAGLAAAQ
QLTRAGHSVTVFERDDRLGGLMRYGVPEYKMENRWIDRRIEQMEAEGTTFQVGTSPRAAELALFDAILLATGTPVARELS
VPGHDLNGIHAAMDYLTAQNRINEGDGEVSPINAKGKKVVIIGGGDTGTDCFGTALRQGAESVTQFDIRPRAPFQRADST
PWPMYPNLFRTATAHEEGEYIITGDESADEIAALGLAERAAGSTLGERKFAVNTVEFHGNNGHVTGLTGNQIRVVNGKRE
PIEGTEFPFEADLVLVALGFTGAEQGGLAHELGVGFDDRGRILRDSEYRSPTNSRVYIAGDNGRGQSLIVWAIAEGRACA
AAIDADLMGETALPVAVAPQDVPLAV
>Mature_505_residues
ADPQGFIKYSRREPAHRPVPLRLMDHSEVYEKAPAGQIEEQAARCMDCGVPFCHEGCPLGNIIPEWNDLVRQGRWKEAYD
RLHATNNFPEFTGRLCPAPCEGACVLGINDDSVTIKNVELEIVEKAFREGWVQPVIPSMSTGLSVAVVGSGPAGLAAAQQ
LTRAGHSVTVFERDDRLGGLMRYGVPEYKMENRWIDRRIEQMEAEGTTFQVGTSPRAAELALFDAILLATGTPVARELSV
PGHDLNGIHAAMDYLTAQNRINEGDGEVSPINAKGKKVVIIGGGDTGTDCFGTALRQGAESVTQFDIRPRAPFQRADSTP
WPMYPNLFRTATAHEEGEYIITGDESADEIAALGLAERAAGSTLGERKFAVNTVEFHGNNGHVTGLTGNQIRVVNGKREP
IEGTEFPFEADLVLVALGFTGAEQGGLAHELGVGFDDRGRILRDSEYRSPTNSRVYIAGDNGRGQSLIVWAIAEGRACAA
AIDADLMGETALPVAVAPQDVPLAV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=298, Percent_Identity=24.1610738255034, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI1789606, Length=498, Percent_Identity=34.136546184739, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI1788811, Length=494, Percent_Identity=33.6032388663968, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI87082180, Length=475, Percent_Identity=30.5263157894737, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789243, Length=265, Percent_Identity=31.3207547169811, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788468, Length=272, Percent_Identity=26.8382352941176, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1789463, Length=210, Percent_Identity=29.5238095238095, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17570289, Length=509, Percent_Identity=43.0255402750491, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI71984108, Length=306, Percent_Identity=23.202614379085, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320030, Length=507, Percent_Identity=41.8145956607495, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI28574881, Length=505, Percent_Identity=46.1386138613861, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24665539, Length=505, Percent_Identity=46.1386138613861, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24665547, Length=505, Percent_Identity=46.1386138613861, Blast_Score=438, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24665543, Length=505, Percent_Identity=46.1386138613861, Blast_Score=438, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24640763, Length=309, Percent_Identity=25.8899676375405, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI18858217, Length=309, Percent_Identity=25.8899676375405, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54575; Mature: 54444

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPQGFIKYSRREPAHRPVPLRLMDHSEVYEKAPAGQIEEQAARCMDCGVPFCHEGCPL
CCCCHHHHHCCCCCCCCCCCCEEECCCHHHHHHCCCCHHHHHHHHHHHCCCCHHCCCCCC
GNIIPEWNDLVRQGRWKEAYDRLHATNNFPEFTGRLCPAPCEGACVLGINDDSVTIKNVE
CCCCCCHHHHHHCCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCEEEEEECCCCEEEEECH
LEIVEKAFREGWVQPVIPSMSTGLSVAVVGSGPAGLAAAQQLTRAGHSVTVFERDDRLGG
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCC
LMRYGVPEYKMENRWIDRRIEQMEAEGTTFQVGTSPRAAELALFDAILLATGTPVARELS
HHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEECC
VPGHDLNGIHAAMDYLTAQNRINEGDGEVSPINAKGKKVVIIGGGDTGTDCFGTALRQGA
CCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHH
ESVTQFDIRPRAPFQRADSTPWPMYPNLFRTATAHEEGEYIITGDESADEIAALGLAERA
HHHHEECCCCCCCHHCCCCCCCCCCCHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHH
AGSTLGERKFAVNTVEFHGNNGHVTGLTGNQIRVVNGKREPIEGTEFPFEADLVLVALGF
CCCCCCCCEEEEEEEEEECCCCEEEEECCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEC
TGAEQGGLAHELGVGFDDRGRILRDSEYRSPTNSRVYIAGDNGRGQSLIVWAIAEGRACA
CCCCCCCCHHHHCCCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCEEE
AAIDADLMGETALPVAVAPQDVPLAV
EHHCHHHCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure 
ADPQGFIKYSRREPAHRPVPLRLMDHSEVYEKAPAGQIEEQAARCMDCGVPFCHEGCPL
CCCHHHHHCCCCCCCCCCCCEEECCCHHHHHHCCCCHHHHHHHHHHHCCCCHHCCCCCC
GNIIPEWNDLVRQGRWKEAYDRLHATNNFPEFTGRLCPAPCEGACVLGINDDSVTIKNVE
CCCCCCHHHHHHCCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCEEEEEECCCCEEEEECH
LEIVEKAFREGWVQPVIPSMSTGLSVAVVGSGPAGLAAAQQLTRAGHSVTVFERDDRLGG
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCC
LMRYGVPEYKMENRWIDRRIEQMEAEGTTFQVGTSPRAAELALFDAILLATGTPVARELS
HHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEECC
VPGHDLNGIHAAMDYLTAQNRINEGDGEVSPINAKGKKVVIIGGGDTGTDCFGTALRQGA
CCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHH
ESVTQFDIRPRAPFQRADSTPWPMYPNLFRTATAHEEGEYIITGDESADEIAALGLAERA
HHHHEECCCCCCCHHCCCCCCCCCCCHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHH
AGSTLGERKFAVNTVEFHGNNGHVTGLTGNQIRVVNGKREPIEGTEFPFEADLVLVALGF
CCCCCCCCEEEEEEEEEECCCCEEEEECCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEC
TGAEQGGLAHELGVGFDDRGRILRDSEYRSPTNSRVYIAGDNGRGQSLIVWAIAEGRACA
CCCCCCCCHHHHCCCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCEEE
AAIDADLMGETALPVAVAPQDVPLAV
EHHCHHHCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]