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The map label for this gene is tcpT [H]

Identifier: 59714051

GI number: 59714051

Start: 980538

End: 982031

Strand: Reverse

Name: tcpT [H]

Synonym: VF_A0868

Alternate gene names: 59714051

Gene position: 982031-980538 (Counterclockwise)

Preceding gene: 59714052

Following gene: 59714050

Centisome position: 73.82

GC content: 31.46

Gene sequence:

>1494_bases
ATGAAAATTAAAATTTTAAAAAAAATCAAAGGTTTTGATTTTTTTAAAGACCACGGTGTAATCTATTGTGAAGTTGATGA
CGTACTATATCAAGCTATATCTGAAAGTGAACTATTAACACTACTCATGGATTCATTTTTTTATGATGATTCAGGAGATG
AAAAATATATTGTAGTTCCCGATGAATATTTTAAGTTATTGGATATAAAATTATTAGAATCTCATAAATTGGATTTGTCA
AAAAAGACAAAATCTGAAGAACGTTGTCTTAATATATTTAAACAAGCAATTATAAATAAAGCTAGTGACGTTCATTTTAT
TAGAAATGATGATACGGCAAAAATTAAATTCAGGATTAATGGAAAAGTAGAATTACAGCAAGAGGTATTAGGAACCGATT
GCGATGAAATGTTATTTGTCATGTATAACGTAATGGCTTCTACAAAAGAAACAACATGGAATACAAAGGCTCCACAAGAT
GCTAATATCCTTTTAGATATCGAAAATGTTAGTTATCGATTCAGATACGCGCACATGCCTATTTTCGGTAAAGGAACATC
AGGTGCTTCATATCATGCTGTTGTACGAGTGATTTATCCGAATGAAAATAAAAATGTATGTACTGATCTTGGCATATTGG
GATTAAGGAAGGATGAAGTTGTTGCTATAGACCATATGCTTTCAAATCCATCGGGGTTAATTGTTGTATCTGGTGTTACT
GGTTCAGGTAAATCTACTGCGTTAAAAAATTATATGGAATATCTTTTTTATACAAAATATCATGAAAAAGGATGTTTTGT
AACAGTTGAAGATCCTGTTGAATATGTAATTGAAGGAGCTCAACAGAGTTCTGTAACAAAATCAAAAAATGGTTCAAACC
TATTTTCTGAAGCGATTCGTTCATCGATGCGTCGTGACCCTGATGTTTTGATGATTGGTGAAATACGAGACCTTCAAACT
GGAAGTGCTTTATCTTCAGCTGTAGAAAGTGGTCACCTTTGTTTAACTACTGTTCATGCAGGTAATTCTGTTGGTGTACT
TCAGCGTTTAGTTGGACTTGGGATTGAGTCTGATAAATTATCTACGCCCGATTTTATTGCTGGAATTATGAATCAAAAAT
TAGTCCCGAGAATTTGTCCTGATTGTGCTCAAGAGTCAATTAATGATTATGGTAGAAAAATAAAAATTCAAGGTGATGGA
TGTGTTGAATGCAATTTTAAAGGTGTCATTGGAAGGATTTTAGTTATTGAACTTATGATTCCTGACTTTAATATTCTTAA
ACTTGTTTCTGAACATAAGTGGGTTGATATATATAGTTATCTAAAAGCGCAACGAAAGCCCGATAATCTTACTCAAGGTT
ATTCCATAAAAGATAAAATTTATTATTTATCTTTAACCGGACAAGTTTGTGGGAATTATTTTAGATCTTATTTTGGGTAT
ATGGAAAATGATGATGAATCGCTTGTTTTTTCAGTTTATCAATCAGAAAAATAG

Upstream 100 bases:

>100_bases
AAACACAAAATGTTAAAGATGGTGGTTATTATTATGGTTGGAGGTTAAAAATAAACAACAGAAATGTCGTTATATCAAAA
GGTTCAAATGCGGTGGCGCT

Downstream 100 bases:

>100_bases
ATTGATTTTATATAGATTGTTATCAGATTTGATTGATGATGGCATTCCAGTATTTGACGCTTTAGTTTTAGTTAAGAATG
AAGGTGGTCAATCTGTTTAT

Product: toxin coregulated pilus biosynthesis protein TcpT

Products: NA

Alternate protein names: TCP pilus biosynthesis protein tcpT [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MKIKILKKIKGFDFFKDHGVIYCEVDDVLYQAISESELLTLLMDSFFYDDSGDEKYIVVPDEYFKLLDIKLLESHKLDLS
KKTKSEERCLNIFKQAIINKASDVHFIRNDDTAKIKFRINGKVELQQEVLGTDCDEMLFVMYNVMASTKETTWNTKAPQD
ANILLDIENVSYRFRYAHMPIFGKGTSGASYHAVVRVIYPNENKNVCTDLGILGLRKDEVVAIDHMLSNPSGLIVVSGVT
GSGKSTALKNYMEYLFYTKYHEKGCFVTVEDPVEYVIEGAQQSSVTKSKNGSNLFSEAIRSSMRRDPDVLMIGEIRDLQT
GSALSSAVESGHLCLTTVHAGNSVGVLQRLVGLGIESDKLSTPDFIAGIMNQKLVPRICPDCAQESINDYGRKIKIQGDG
CVECNFKGVIGRILVIELMIPDFNILKLVSEHKWVDIYSYLKAQRKPDNLTQGYSIKDKIYYLSLTGQVCGNYFRSYFGY
MENDDESLVFSVYQSEK

Sequences:

>Translated_497_residues
MKIKILKKIKGFDFFKDHGVIYCEVDDVLYQAISESELLTLLMDSFFYDDSGDEKYIVVPDEYFKLLDIKLLESHKLDLS
KKTKSEERCLNIFKQAIINKASDVHFIRNDDTAKIKFRINGKVELQQEVLGTDCDEMLFVMYNVMASTKETTWNTKAPQD
ANILLDIENVSYRFRYAHMPIFGKGTSGASYHAVVRVIYPNENKNVCTDLGILGLRKDEVVAIDHMLSNPSGLIVVSGVT
GSGKSTALKNYMEYLFYTKYHEKGCFVTVEDPVEYVIEGAQQSSVTKSKNGSNLFSEAIRSSMRRDPDVLMIGEIRDLQT
GSALSSAVESGHLCLTTVHAGNSVGVLQRLVGLGIESDKLSTPDFIAGIMNQKLVPRICPDCAQESINDYGRKIKIQGDG
CVECNFKGVIGRILVIELMIPDFNILKLVSEHKWVDIYSYLKAQRKPDNLTQGYSIKDKIYYLSLTGQVCGNYFRSYFGY
MENDDESLVFSVYQSEK
>Mature_497_residues
MKIKILKKIKGFDFFKDHGVIYCEVDDVLYQAISESELLTLLMDSFFYDDSGDEKYIVVPDEYFKLLDIKLLESHKLDLS
KKTKSEERCLNIFKQAIINKASDVHFIRNDDTAKIKFRINGKVELQQEVLGTDCDEMLFVMYNVMASTKETTWNTKAPQD
ANILLDIENVSYRFRYAHMPIFGKGTSGASYHAVVRVIYPNENKNVCTDLGILGLRKDEVVAIDHMLSNPSGLIVVSGVT
GSGKSTALKNYMEYLFYTKYHEKGCFVTVEDPVEYVIEGAQQSSVTKSKNGSNLFSEAIRSSMRRDPDVLMIGEIRDLQT
GSALSSAVESGHLCLTTVHAGNSVGVLQRLVGLGIESDKLSTPDFIAGIMNQKLVPRICPDCAQESINDYGRKIKIQGDG
CVECNFKGVIGRILVIELMIPDFNILKLVSEHKWVDIYSYLKAQRKPDNLTQGYSIKDKIYYLSLTGQVCGNYFRSYFGY
MENDDESLVFSVYQSEK

Specific function: Involved in the translocation of the tcpA pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=420, Percent_Identity=29.5238095238095, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI1786296, Length=362, Percent_Identity=27.9005524861878, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI87082188, Length=158, Percent_Identity=34.8101265822785, Blast_Score=93, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 56107; Mature: 56107

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKILKKIKGFDFFKDHGVIYCEVDDVLYQAISESELLTLLMDSFFYDDSGDEKYIVVP
CCEEEEHHHCCCCEECCCCEEEEEHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCEEEEEC
DEYFKLLDIKLLESHKLDLSKKTKSEERCLNIFKQAIINKASDVHFIRNDDTAKIKFRIN
HHHHHHHHHEEHHCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEEC
GKVELQQEVLGTDCDEMLFVMYNVMASTKETTWNTKAPQDANILLDIENVSYRFRYAHMP
CCEEEHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCEEEEEEEECE
IFGKGTSGASYHAVVRVIYPNENKNVCTDLGILGLRKDEVVAIDHMLSNPSGLIVVSGVT
EEECCCCCCCEEEEEEEEECCCCCCCCHHCCEEECCCCCEEEEEHHHCCCCCEEEEEECC
GSGKSTALKNYMEYLFYTKYHEKGCFVTVEDPVEYVIEGAQQSSVTKSKNGSNLFSEAIR
CCCCHHHHHHHHHHHHHHEECCCCCEEEECCHHHHHHCCCHHHCCCCCCCCCHHHHHHHH
SSMRRDPDVLMIGEIRDLQTGSALSSAVESGHLCLTTVHAGNSVGVLQRLVGLGIESDKL
HHHCCCCCEEEEECCCCCCCCHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCC
STPDFIAGIMNQKLVPRICPDCAQESINDYGRKIKIQGDGCVECNFKGVIGRILVIELMI
CCHHHHHHHHCCCCCHHCCHHHHHHHHHHCCCEEEECCCCEEEECCHHHHHEEEEEEEEC
PDFNILKLVSEHKWVDIYSYLKAQRKPDNLTQGYSIKDKIYYLSLTGQVCGNYFRSYFGY
CCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEECEEEEEEECHHHHHHHHHHHCCC
MENDDESLVFSVYQSEK
CCCCCCEEEEEEEECCC
>Mature Secondary Structure
MKIKILKKIKGFDFFKDHGVIYCEVDDVLYQAISESELLTLLMDSFFYDDSGDEKYIVVP
CCEEEEHHHCCCCEECCCCEEEEEHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCEEEEEC
DEYFKLLDIKLLESHKLDLSKKTKSEERCLNIFKQAIINKASDVHFIRNDDTAKIKFRIN
HHHHHHHHHEEHHCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEEC
GKVELQQEVLGTDCDEMLFVMYNVMASTKETTWNTKAPQDANILLDIENVSYRFRYAHMP
CCEEEHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCEEEEEEEECE
IFGKGTSGASYHAVVRVIYPNENKNVCTDLGILGLRKDEVVAIDHMLSNPSGLIVVSGVT
EEECCCCCCCEEEEEEEEECCCCCCCCHHCCEEECCCCCEEEEEHHHCCCCCEEEEEECC
GSGKSTALKNYMEYLFYTKYHEKGCFVTVEDPVEYVIEGAQQSSVTKSKNGSNLFSEAIR
CCCCHHHHHHHHHHHHHHEECCCCCEEEECCHHHHHHCCCHHHCCCCCCCCCHHHHHHHH
SSMRRDPDVLMIGEIRDLQTGSALSSAVESGHLCLTTVHAGNSVGVLQRLVGLGIESDKL
HHHCCCCCEEEEECCCCCCCCHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCC
STPDFIAGIMNQKLVPRICPDCAQESINDYGRKIKIQGDGCVECNFKGVIGRILVIELMI
CCHHHHHHHHCCCCCHHCCHHHHHHHHHHCCCEEEECCCCEEEECCHHHHHEEEEEEEEC
PDFNILKLVSEHKWVDIYSYLKAQRKPDNLTQGYSIKDKIYYLSLTGQVCGNYFRSYFGY
CCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEECEEEEEEECHHHHHHHHHHHCCC
MENDDESLVFSVYQSEK
CCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8097178; 10952301 [H]