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The map label for this gene is putP [H]

Identifier: 59714012

GI number: 59714012

Start: 931434

End: 932930

Strand: Reverse

Name: putP [H]

Synonym: VF_A0829

Alternate gene names: 59714012

Gene position: 932930-931434 (Counterclockwise)

Preceding gene: 59714013

Following gene: 59714011

Centisome position: 70.13

GC content: 40.15

Gene sequence:

>1497_bases
ATGATTGAAAATAACTTTGCCATTACAGGCACTTTTATCGCTTACTTGATAATGATGTTAGCGATTGGGGTATACGCATA
TAAACGAACGAGTAGCTCATCAGATTACTTTTTGGGTGGACGAACATTAGGTCCATGGCCGGCAGCATTGTCAGCTGGTG
CGTCAGATATGAGTGGTTGGTTACTGCTTGGTTTACCTGGTTATGCTTATGCAGCTGGTTTAGAGTCGTTGTGGCTTGCT
GGTGGTTTACTTGTTGGTACATGGCTAAACTGGTTAATTTGTGCAAAACGCTTAAGAACCTATAGCATGACAACGGATGA
CTCATTAACTCTTCCTGAATTTTTGTCCCGTCGTTTTAATGACAAATCGAAACTCATTCAAACCATTTCTGCTCTATTCA
TTTTACTTTTCTTCCTTTTCTATACGAGTTCTGGTTTGGTTGCTGGTGGTAAGTTATTTGAGACTGTATTTGGTTTAGAT
TACAAAGTGGCTGTGGTAATTGGAACATTATGTGTCGTCTCATATACCTTATTCGGTGGATTTTTAGCTGTTGCATGGAC
CGATCTTGTTCAAGGCCTTTTAATGTCTGCTGCGTTGATGATTGTACCTATTGCAGCTATGCAAGGTGGCTTCGGACAAT
TGAGTTCAGAGCTTGTCGCAATTAACCCTGAGTTAATGACTTTATGGACAGGAATGGATGGCAAACCATTAAGTGCGATT
GCAATTATTTCATTAGTGGCATGGGGACTTGGTTATTTTGGACAACCACATATTTTGGCTCGTTTCCAAGCATCTCGTAC
AAATAAAGATTTAACGACAGCGCGTCGTATTGCCGTTGCATGGACCGCTCTATCCATGTTTGGCGCCGTACTTGTTGGTC
TTGTTGGTTTACTATATGTAACAGGTTCTATGCCTGGTGAGACGATTGATGGTGAAAAAGTATTTATGCTGTTAGTAAAT
GCCATTTTCCATCCGGTTGTTGCTGGTATTTTATTAGCTGCTATTTTAGCGGCAATAATGAGTACTGCGGATTCGCAATT
ACTTGTATCTTCTTCGGCGTTATCAGAAGATTTATATAAGCAACTGGTTAAGAAAGACGCAACAAGCAAAGAGATCGTTA
ATGTGGGTCGTTTCGCTGTCGTTGCTTTGTCTGTTATCGCTCTATTATTAGCAATGAATCCTGATAGTTCTGTTCTTGGT
CTCGTATCTTACGCATGGGCAGGTTTTGGTGCTGCATTTGGTCCAGCGATTGTACTTAGCCTTTATTGGAAAGGAATGAA
TCGTAATGGTGCTCTTGCTGGCATTATCGTTGGTGGTGTAACAATTGTTGTTTGGAAGCAATTAACTGGCGGTTTATTTG
ATGTCTATGAGATTGTTCCTGGAATTATCTTTGCAACTATTGCGATTATTGTCGTTAGTAAGTTAACTGGTAAGCCTGAA
GATAAAGTACTAAAACAGCATGAAGAGTTTGAACGTAACCTAGTTGAGTTTGAATAA

Upstream 100 bases:

>100_bases
GGATGAATATTACACCGTCATTTTTGCGCCATAACTATAGAGCAAAGCTCAACATAACAAGAGTGACGTTAATTACTGCC
AATAACACAAAGGAAATAAA

Downstream 100 bases:

>100_bases
GTTCTAGCTTTTTAACAACGATAAACTTAAAGCCTCATAAGATGATTTCTTTTGAGGCTTTTTTTTATATAAATAAAACT
GTTACAATCCAGTAACTTTA

Product: proline:sodium symporter

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MIENNFAITGTFIAYLIMMLAIGVYAYKRTSSSSDYFLGGRTLGPWPAALSAGASDMSGWLLLGLPGYAYAAGLESLWLA
GGLLVGTWLNWLICAKRLRTYSMTTDDSLTLPEFLSRRFNDKSKLIQTISALFILLFFLFYTSSGLVAGGKLFETVFGLD
YKVAVVIGTLCVVSYTLFGGFLAVAWTDLVQGLLMSAALMIVPIAAMQGGFGQLSSELVAINPELMTLWTGMDGKPLSAI
AIISLVAWGLGYFGQPHILARFQASRTNKDLTTARRIAVAWTALSMFGAVLVGLVGLLYVTGSMPGETIDGEKVFMLLVN
AIFHPVVAGILLAAILAAIMSTADSQLLVSSSALSEDLYKQLVKKDATSKEIVNVGRFAVVALSVIALLLAMNPDSSVLG
LVSYAWAGFGAAFGPAIVLSLYWKGMNRNGALAGIIVGGVTIVVWKQLTGGLFDVYEIVPGIIFATIAIIVVSKLTGKPE
DKVLKQHEEFERNLVEFE

Sequences:

>Translated_498_residues
MIENNFAITGTFIAYLIMMLAIGVYAYKRTSSSSDYFLGGRTLGPWPAALSAGASDMSGWLLLGLPGYAYAAGLESLWLA
GGLLVGTWLNWLICAKRLRTYSMTTDDSLTLPEFLSRRFNDKSKLIQTISALFILLFFLFYTSSGLVAGGKLFETVFGLD
YKVAVVIGTLCVVSYTLFGGFLAVAWTDLVQGLLMSAALMIVPIAAMQGGFGQLSSELVAINPELMTLWTGMDGKPLSAI
AIISLVAWGLGYFGQPHILARFQASRTNKDLTTARRIAVAWTALSMFGAVLVGLVGLLYVTGSMPGETIDGEKVFMLLVN
AIFHPVVAGILLAAILAAIMSTADSQLLVSSSALSEDLYKQLVKKDATSKEIVNVGRFAVVALSVIALLLAMNPDSSVLG
LVSYAWAGFGAAFGPAIVLSLYWKGMNRNGALAGIIVGGVTIVVWKQLTGGLFDVYEIVPGIIFATIAIIVVSKLTGKPE
DKVLKQHEEFERNLVEFE
>Mature_498_residues
MIENNFAITGTFIAYLIMMLAIGVYAYKRTSSSSDYFLGGRTLGPWPAALSAGASDMSGWLLLGLPGYAYAAGLESLWLA
GGLLVGTWLNWLICAKRLRTYSMTTDDSLTLPEFLSRRFNDKSKLIQTISALFILLFFLFYTSSGLVAGGKLFETVFGLD
YKVAVVIGTLCVVSYTLFGGFLAVAWTDLVQGLLMSAALMIVPIAAMQGGFGQLSSELVAINPELMTLWTGMDGKPLSAI
AIISLVAWGLGYFGQPHILARFQASRTNKDLTTARRIAVAWTALSMFGAVLVGLVGLLYVTGSMPGETIDGEKVFMLLVN
AIFHPVVAGILLAAILAAIMSTADSQLLVSSSALSEDLYKQLVKKDATSKEIVNVGRFAVVALSVIALLLAMNPDSSVLG
LVSYAWAGFGAAFGPAIVLSLYWKGMNRNGALAGIIVGGVTIVVWKQLTGGLFDVYEIVPGIIFATIAIIVVSKLTGKPE
DKVLKQHEEFERNLVEFE

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=489, Percent_Identity=46.0122699386503, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI14140236, Length=480, Percent_Identity=24.375, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI4507035, Length=385, Percent_Identity=26.7532467532467, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI110835708, Length=506, Percent_Identity=23.3201581027668, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI109659836, Length=487, Percent_Identity=24.6406570841889, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI11141885, Length=458, Percent_Identity=24.8908296943231, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI4507031, Length=481, Percent_Identity=23.2848232848233, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1787251, Length=488, Percent_Identity=48.3606557377049, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI87082237, Length=492, Percent_Identity=30.4878048780488, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1790503, Length=466, Percent_Identity=29.3991416309013, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI1790113, Length=414, Percent_Identity=22.9468599033816, Blast_Score=72, Evalue=6e-14,
Organism=Drosophila melanogaster, GI221459584, Length=385, Percent_Identity=24.4155844155844, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24651741, Length=216, Percent_Identity=29.1666666666667, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24645928, Length=389, Percent_Identity=26.2210796915167, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648033, Length=454, Percent_Identity=23.3480176211454, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21356865, Length=454, Percent_Identity=23.3480176211454, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221459588, Length=387, Percent_Identity=24.5478036175711, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24651739, Length=202, Percent_Identity=27.2277227722772, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 53320; Mature: 53320

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIENNFAITGTFIAYLIMMLAIGVYAYKRTSSSSDYFLGGRTLGPWPAALSAGASDMSGW
CCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHCCCCCCCCE
LLLGLPGYAYAAGLESLWLAGGLLVGTWLNWLICAKRLRTYSMTTDDSLTLPEFLSRRFN
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCC
DKSKLIQTISALFILLFFLFYTSSGLVAGGKLFETVFGLDYKVAVVIGTLCVVSYTLFGG
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
FLAVAWTDLVQGLLMSAALMIVPIAAMQGGFGQLSSELVAINPELMTLWTGMDGKPLSAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEECCHHEEEEECCCCCCHHHH
AIISLVAWGLGYFGQPHILARFQASRTNKDLTTARRIAVAWTALSMFGAVLVGLVGLLYV
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGSMPGETIDGEKVFMLLVNAIFHPVVAGILLAAILAAIMSTADSQLLVSSSALSEDLYK
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QLVKKDATSKEIVNVGRFAVVALSVIALLLAMNPDSSVLGLVSYAWAGFGAAFGPAIVLS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
LYWKGMNRNGALAGIIVGGVTIVVWKQLTGGLFDVYEIVPGIIFATIAIIVVSKLTGKPE
HHHHCCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
DKVLKQHEEFERNLVEFE
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIENNFAITGTFIAYLIMMLAIGVYAYKRTSSSSDYFLGGRTLGPWPAALSAGASDMSGW
CCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHCCCCCCCCE
LLLGLPGYAYAAGLESLWLAGGLLVGTWLNWLICAKRLRTYSMTTDDSLTLPEFLSRRFN
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCC
DKSKLIQTISALFILLFFLFYTSSGLVAGGKLFETVFGLDYKVAVVIGTLCVVSYTLFGG
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
FLAVAWTDLVQGLLMSAALMIVPIAAMQGGFGQLSSELVAINPELMTLWTGMDGKPLSAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEECCHHEEEEECCCCCCHHHH
AIISLVAWGLGYFGQPHILARFQASRTNKDLTTARRIAVAWTALSMFGAVLVGLVGLLYV
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGSMPGETIDGEKVFMLLVNAIFHPVVAGILLAAILAAIMSTADSQLLVSSSALSEDLYK
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QLVKKDATSKEIVNVGRFAVVALSVIALLLAMNPDSSVLGLVSYAWAGFGAAFGPAIVLS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
LYWKGMNRNGALAGIIVGGVTIVVWKQLTGGLFDVYEIVPGIIFATIAIIVVSKLTGKPE
HHHHCCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
DKVLKQHEEFERNLVEFE
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]