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The map label for this gene is sspA [C]

Identifier: 59714004

GI number: 59714004

Start: 924577

End: 925287

Strand: Reverse

Name: sspA [C]

Synonym: VF_A0821

Alternate gene names: 59714004

Gene position: 925287-924577 (Counterclockwise)

Preceding gene: 59714005

Following gene: 59714003

Centisome position: 69.55

GC content: 38.4

Gene sequence:

>711_bases
ATGATTAAATTAATTAGCTTTAACAATTGCCCATTTGTACAACGTGTAATGGGAGCGTTAGTGACAAAGAATGTGGCGTT
TGAAATTGAATACATTGAATTGAATAATAAACCACAGTGGTTCTTGGATATTGCGCCAAATGGTCAAGTGCCAGTATTGA
TCACAGAAGATAAGACGGTGTTGTTTGAGTCGGATGCTATTGTTGAATATCTTGATGATAAATACGCGCCAATTGAAGAT
CTTACACCAGAACAGCGAGCAATTGATCGTGCATGGTCATATCAAGCGAGTAAGCATTACATGCCGCAATGTGGAACCAT
GGGAAGTAAAGACAAAGAAACTTTTGAGACACGTTTGGCTAATCTTTCTAAAGCGTTCGCTAAGGCGGAAGGTAAATTGA
GTGATTCATTGTTTTTTAAAGGTGACTATATCTCAAATGTTGATATTGCTTGGCTTCCATTGTTACATCGTGCAGCTGTC
ATTAAAGATCAATCAGGGTTTGATATGCTTGATGGCTTCCCTAAAGTTCAAAAATGGCAGGCTTCACTTATTGAATCTGG
TCTTGCAGAGAAAACAGTACCGCAAGATTTTGTCGCAACCTTTAGTGGCTTTTATTTAAAAAATACCTATTTAGCTCATG
TAATGAATGGTACAGAGGCAGAATGTGCAGAAGGACGCTGTTCAACGGATTCAAGCTCATGCTGTAATTAG

Upstream 100 bases:

>100_bases
ATCTTTGATGGGGATTTTTTTTATTAAAATGTCGTCTTTTCTAAATCGGTTTCGTCTTATAGATGTAAATACAAATAACG
AACCACTTTGGAGTAACACC

Downstream 100 bases:

>100_bases
TTGTATTCATAGCAACAAATAGTAAACTTTAATCAAGGTAACCAACTATTGGTTACCTTAAGTTGTTTGTACCGCCAAAA
AATTAGAAAGTATAAAGGTA

Product: glutathione S-transferase

Products: HX; R-S-glutathione

Alternate protein names: Glutathione S-Transferase Domain-Containing Protein; Glutathione S-Transferase Domain Protein; Glutathione S-Transferase Protein; Glutathione S-Transferase-Like Protein; Stringent Starvation Protein A; Glutathione S-Transferase Domain; Glutathione-S-Transferase; Glutathione S Transferase; Glutathione-S-Transferase Protein; Glutathione S-Transferase-Like; Glucose-Inhibited Division Protein A Subfamily Protein; Glutathione S-Transferase Family Protein

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MIKLISFNNCPFVQRVMGALVTKNVAFEIEYIELNNKPQWFLDIAPNGQVPVLITEDKTVLFESDAIVEYLDDKYAPIED
LTPEQRAIDRAWSYQASKHYMPQCGTMGSKDKETFETRLANLSKAFAKAEGKLSDSLFFKGDYISNVDIAWLPLLHRAAV
IKDQSGFDMLDGFPKVQKWQASLIESGLAEKTVPQDFVATFSGFYLKNTYLAHVMNGTEAECAEGRCSTDSSSCCN

Sequences:

>Translated_236_residues
MIKLISFNNCPFVQRVMGALVTKNVAFEIEYIELNNKPQWFLDIAPNGQVPVLITEDKTVLFESDAIVEYLDDKYAPIED
LTPEQRAIDRAWSYQASKHYMPQCGTMGSKDKETFETRLANLSKAFAKAEGKLSDSLFFKGDYISNVDIAWLPLLHRAAV
IKDQSGFDMLDGFPKVQKWQASLIESGLAEKTVPQDFVATFSGFYLKNTYLAHVMNGTEAECAEGRCSTDSSSCCN
>Mature_236_residues
MIKLISFNNCPFVQRVMGALVTKNVAFEIEYIELNNKPQWFLDIAPNGQVPVLITEDKTVLFESDAIVEYLDDKYAPIED
LTPEQRAIDRAWSYQASKHYMPQCGTMGSKDKETFETRLANLSKAFAKAEGKLSDSLFFKGDYISNVDIAWLPLLHRAAV
IKDQSGFDMLDGFPKVQKWQASLIESGLAEKTVPQDFVATFSGFYLKNTYLAHVMNGTEAECAEGRCSTDSSSCCN

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4758484, Length=189, Percent_Identity=30.6878306878307, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI300360534, Length=181, Percent_Identity=30.939226519337, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI300360532, Length=184, Percent_Identity=29.3478260869565, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1789624, Length=148, Percent_Identity=29.0540540540541, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.5.1.18

Molecular weight: Translated: 26470; Mature: 26470

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLISFNNCPFVQRVMGALVTKNVAFEIEYIELNNKPQWFLDIAPNGQVPVLITEDKTV
CEEEEECCCCCHHHHHHHHHHHCCEEEEEEEEEECCCCCEEEEECCCCCEEEEEECCCEE
LFESDAIVEYLDDKYAPIEDLTPEQRAIDRAWSYQASKHYMPQCGTMGSKDKETFETRLA
EEECCHHHHHHCCCCCCHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
NLSKAFAKAEGKLSDSLFFKGDYISNVDIAWLPLLHRAAVIKDQSGFDMLDGFPKVQKWQ
HHHHHHHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHH
ASLIESGLAEKTVPQDFVATFSGFYLKNTYLAHVMNGTEAECAEGRCSTDSSSCCN
HHHHHCCCHHCCCCHHHHHHHHCHHHHHHEEEEECCCCCCHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MIKLISFNNCPFVQRVMGALVTKNVAFEIEYIELNNKPQWFLDIAPNGQVPVLITEDKTV
CEEEEECCCCCHHHHHHHHHHHCCEEEEEEEEEECCCCCEEEEECCCCCEEEEEECCCEE
LFESDAIVEYLDDKYAPIEDLTPEQRAIDRAWSYQASKHYMPQCGTMGSKDKETFETRLA
EEECCHHHHHHCCCCCCHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
NLSKAFAKAEGKLSDSLFFKGDYISNVDIAWLPLLHRAAVIKDQSGFDMLDGFPKVQKWQ
HHHHHHHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHH
ASLIESGLAEKTVPQDFVATFSGFYLKNTYLAHVMNGTEAECAEGRCSTDSSSCCN
HHHHHCCCHHCCCCHHHHHHHHCHHHHHHEEEEECCCCCCHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA