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The map label for this gene is glgB

Identifier: 59713991

GI number: 59713991

Start: 908344

End: 910491

Strand: Reverse

Name: glgB

Synonym: VF_A0808

Alternate gene names: 59713991

Gene position: 910491-908344 (Counterclockwise)

Preceding gene: 172087815

Following gene: 59713989

Centisome position: 68.44

GC content: 40.13

Gene sequence:

>2148_bases
ATGCAGCTCGAAAGGGCTGCTTTTTCTGATCCATTTTCTTTTCTAGGTCCACAATATCAAAGTCAAACGACAGCACTACG
AGTTTGGCTTCCAGGTGCTACATCGGTAAAAGTACGATTATCTGGTCATGTTGAATATCAATTGCTTTCAGACCCGAGAC
ATTCGGGTATTTTTGTATTAAATGAAAGCATTGATATGACAGAAGTTCATTATGAATTAATTATCGATTGGAGTGGAACT
GAACAAATTCTTGATGACCCATATCAATACCACGATATTTCCCCTACGGATGCGCAAGTTCATACACCAAAAGAGATGTA
TAACCATTTAGGGGCGCATCTATTTAACGTTGTTCGTGACGGTAAGCAAATTCAAGGCGTTCGTTATTTAGTCTTTGCCC
CTAATGCCTCTTCAGCTAGCGTTATTGGTGATTTTAACCAGTGGGATGGCCGTCGTCATATTATGCAACGTATAGATAAC
GGACTTTGGGCGTTATTTATACCTGAACATGCTGTTGGTACTAAGTATAAATTTGAGTTAAAAGGACCAAATGGAGAATC
ACTTCCTCATAAAATGGATCCTTATGGCGCTCATAATGAGCAGTATCCATCATTTGCATCTGTGGTATATGATCAAACTT
CTTATCAGTGGAATGATGCCAAATGGCAGCAAAGACCAGTAACTGAAAAGCAGAAAGAAGCGCTTTCATTCTATGAATTG
CATGCAGGGTCTTGGAAGAGAAATGAAAATGGCGATTTCTTAACCTATCGTGAGTTAGCTGAGCAATTAATACCTTATAT
TTTAGATATGGGTTATACGCACATAGAGCTAATGCCAGTATCAGAACATCCATTTTATGGTTCATGGGGCTATCAACCAA
TTGGGCTATTTTCACCAACGAGTCGTTTTGGTACGCCTGATGATTTTAAATATTTTGTCGATCAATGCCACCAAGTTGGT
ATTGGTGTCGTACTAGATTGGGTTCCTGCTCATTTCCCATCGGATTCACATGGTTTGGCGAATTTTGATGGAACATCTTT
GTTTAATGATCCTGACCCTCGTCGTGGATGGCACAATGATTGGCAGAGCTTTATTTACAATTATGACCAACCTCATGTGC
GTGAATTTTTAGTTTCTAATGCATTATATTGGTTTGAGCATTTCCATATCGATGGTTTGCGTGTCGATGCTGTAGCATCA
ATGCTTTACCTTGATTATTCGCGTAATGATGGCGAATGGATCCCTAACTGGGAAGGTGGAAATCATAACCACGGAGCCAT
CGCTTTATTAAAGTGGATGAACGAAGAGGTGTATTCACATTATCCTAATGCGATGACCATTGCGGAAGAATCAACGGCTT
TTCCTGGGGTTTCAGCTCCTACTTTTGCTGGTGGTTTGGGCTTTGGCTTTAAGTGGAACATGGGTTGGATGCATGACAGC
TTAAATTACATTAGAGAAGACCCAATTCATCGAAAATATCACCACGATACCATCACTTTCCCGCTAGTTTATGCATTTAG
TGAGAACTTTATCCTTTCGCTTTCACACGATGAAGTGGTTTATGGAAAAGGCTCAATCTTAGATAAAATGCCTGGAGATG
AATGGCAAAAAACGGCAAACTTACGTGCCTATATGGGGTATATGTATGGTCAGCCGGGTAAGAAATTAAACTTCATGGGG
GCTGAAATAGCTCAAAGTGCAGAGTGGGATCATGATGGTCAATTACAGTGGTTCTTAACTCAATTTGAGCGTCACTCTGG
TATGCAATCGCTAGTGCGTGATTTAAATAAATTATACACAACAGAGCCTGCGCTTTATCAAAAAGATTGTGAGCCTGCTG
GTTTTGAGTGGCGACTACAAGATGAAGCAGAAATGAGTGTTCTAGCTCATGAACGTTTAGGTGATAATGGTGAGCGAATT
CTCGTTGTTAGTAACTTTACACCAGCACCAAGAGAGGATTTCCGTTTAGGAATGCCTGTTGCTGGTCAGTATGAGTTGAT
TTTAAACAGTGATGCTCATTTTTATGGGGGCAGTGATTACTCTGTAATTAGTGAAGCATCTACAGAGAAGGTTGAGTCTC
AAGGATTAGCACAATCTATTGTTATTACGTTACCACCGCTTTCTACGGTCTTTTATCGTTTAAAATAA

Upstream 100 bases:

>100_bases
TAACCAAACGATTAACCGACATCCGCGCTCAAGCAAGCCAAAAATAAAAGGAGTATTTACGTTGAACTTACTTGTTGAAC
GTAAAGAAAAGGACATCTAC

Downstream 100 bases:

>100_bases
CAAATCATAAGTATGAATGAAAAAAGCCCTCAAGTCAGTTTCTGATTTAGGGCTTTTTATTAAAAGAATTAGGAGTCTGT
TCTTTATTTGAATTGATATT

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 715; Mature: 715

Protein sequence:

>715_residues
MQLERAAFSDPFSFLGPQYQSQTTALRVWLPGATSVKVRLSGHVEYQLLSDPRHSGIFVLNESIDMTEVHYELIIDWSGT
EQILDDPYQYHDISPTDAQVHTPKEMYNHLGAHLFNVVRDGKQIQGVRYLVFAPNASSASVIGDFNQWDGRRHIMQRIDN
GLWALFIPEHAVGTKYKFELKGPNGESLPHKMDPYGAHNEQYPSFASVVYDQTSYQWNDAKWQQRPVTEKQKEALSFYEL
HAGSWKRNENGDFLTYRELAEQLIPYILDMGYTHIELMPVSEHPFYGSWGYQPIGLFSPTSRFGTPDDFKYFVDQCHQVG
IGVVLDWVPAHFPSDSHGLANFDGTSLFNDPDPRRGWHNDWQSFIYNYDQPHVREFLVSNALYWFEHFHIDGLRVDAVAS
MLYLDYSRNDGEWIPNWEGGNHNHGAIALLKWMNEEVYSHYPNAMTIAEESTAFPGVSAPTFAGGLGFGFKWNMGWMHDS
LNYIREDPIHRKYHHDTITFPLVYAFSENFILSLSHDEVVYGKGSILDKMPGDEWQKTANLRAYMGYMYGQPGKKLNFMG
AEIAQSAEWDHDGQLQWFLTQFERHSGMQSLVRDLNKLYTTEPALYQKDCEPAGFEWRLQDEAEMSVLAHERLGDNGERI
LVVSNFTPAPREDFRLGMPVAGQYELILNSDAHFYGGSDYSVISEASTEKVESQGLAQSIVITLPPLSTVFYRLK

Sequences:

>Translated_715_residues
MQLERAAFSDPFSFLGPQYQSQTTALRVWLPGATSVKVRLSGHVEYQLLSDPRHSGIFVLNESIDMTEVHYELIIDWSGT
EQILDDPYQYHDISPTDAQVHTPKEMYNHLGAHLFNVVRDGKQIQGVRYLVFAPNASSASVIGDFNQWDGRRHIMQRIDN
GLWALFIPEHAVGTKYKFELKGPNGESLPHKMDPYGAHNEQYPSFASVVYDQTSYQWNDAKWQQRPVTEKQKEALSFYEL
HAGSWKRNENGDFLTYRELAEQLIPYILDMGYTHIELMPVSEHPFYGSWGYQPIGLFSPTSRFGTPDDFKYFVDQCHQVG
IGVVLDWVPAHFPSDSHGLANFDGTSLFNDPDPRRGWHNDWQSFIYNYDQPHVREFLVSNALYWFEHFHIDGLRVDAVAS
MLYLDYSRNDGEWIPNWEGGNHNHGAIALLKWMNEEVYSHYPNAMTIAEESTAFPGVSAPTFAGGLGFGFKWNMGWMHDS
LNYIREDPIHRKYHHDTITFPLVYAFSENFILSLSHDEVVYGKGSILDKMPGDEWQKTANLRAYMGYMYGQPGKKLNFMG
AEIAQSAEWDHDGQLQWFLTQFERHSGMQSLVRDLNKLYTTEPALYQKDCEPAGFEWRLQDEAEMSVLAHERLGDNGERI
LVVSNFTPAPREDFRLGMPVAGQYELILNSDAHFYGGSDYSVISEASTEKVESQGLAQSIVITLPPLSTVFYRLK
>Mature_715_residues
MQLERAAFSDPFSFLGPQYQSQTTALRVWLPGATSVKVRLSGHVEYQLLSDPRHSGIFVLNESIDMTEVHYELIIDWSGT
EQILDDPYQYHDISPTDAQVHTPKEMYNHLGAHLFNVVRDGKQIQGVRYLVFAPNASSASVIGDFNQWDGRRHIMQRIDN
GLWALFIPEHAVGTKYKFELKGPNGESLPHKMDPYGAHNEQYPSFASVVYDQTSYQWNDAKWQQRPVTEKQKEALSFYEL
HAGSWKRNENGDFLTYRELAEQLIPYILDMGYTHIELMPVSEHPFYGSWGYQPIGLFSPTSRFGTPDDFKYFVDQCHQVG
IGVVLDWVPAHFPSDSHGLANFDGTSLFNDPDPRRGWHNDWQSFIYNYDQPHVREFLVSNALYWFEHFHIDGLRVDAVAS
MLYLDYSRNDGEWIPNWEGGNHNHGAIALLKWMNEEVYSHYPNAMTIAEESTAFPGVSAPTFAGGLGFGFKWNMGWMHDS
LNYIREDPIHRKYHHDTITFPLVYAFSENFILSLSHDEVVYGKGSILDKMPGDEWQKTANLRAYMGYMYGQPGKKLNFMG
AEIAQSAEWDHDGQLQWFLTQFERHSGMQSLVRDLNKLYTTEPALYQKDCEPAGFEWRLQDEAEMSVLAHERLGDNGERI
LVVSNFTPAPREDFRLGMPVAGQYELILNSDAHFYGGSDYSVISEASTEKVESQGLAQSIVITLPPLSTVFYRLK

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=638, Percent_Identity=26.9592476489028, Blast_Score=189, Evalue=9e-48,
Organism=Escherichia coli, GI1789839, Length=718, Percent_Identity=47.6323119777159, Blast_Score=640, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=597, Percent_Identity=28.4757118927973, Blast_Score=199, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI32564391, Length=578, Percent_Identity=27.681660899654, Blast_Score=181, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6320826, Length=673, Percent_Identity=27.1916790490342, Blast_Score=174, Evalue=4e-44,
Organism=Drosophila melanogaster, GI28573410, Length=639, Percent_Identity=26.4475743348983, Blast_Score=183, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_VIBF1 (Q5DZB8)

Other databases:

- EMBL:   CP000021
- RefSeq:   YP_206766.1
- ProteinModelPortal:   Q5DZB8
- STRING:   Q5DZB8
- GeneID:   3280626
- GenomeReviews:   CP000021_GR
- KEGG:   vfi:VF_A0808
- NMPDR:   fig|312309.3.peg.3725
- eggNOG:   COG0296
- HOGENOM:   HBG287139
- OMA:   DGTCLYE
- PhylomeDB:   Q5DZB8
- ProtClustDB:   PRK05402
- BioCyc:   VFIS312309:VFA0808-MONOMER
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 82044; Mature: 82044

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: ACT_SITE 291-291 ACT_SITE 326-326 ACT_SITE 331-331 ACT_SITE 394-394 ACT_SITE 396-396 ACT_SITE 449-449 ACT_SITE 516-516 ACT_SITE 517-517

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLERAAFSDPFSFLGPQYQSQTTALRVWLPGATSVKVRLSGHVEYQLLSDPRHSGIFVL
CCCCCCCCCCCHHHHCCCCCCCCEEEEEEECCCCEEEEEEECCEEEEEECCCCCCCEEEE
NESIDMTEVHYELIIDWSGTEQILDDPYQYHDISPTDAQVHTPKEMYNHLGAHLFNVVRD
ECCCCCEEEEEEEEEECCCCHHHHCCCHHCCCCCCCCCCEECHHHHHHHHHHHHHHHHHC
GKQIQGVRYLVFAPNASSASVIGDFNQWDGRRHIMQRIDNGLWALFIPEHAVGTKYKFEL
CCCCCCEEEEEECCCCCCCCEEECCHHCCCHHHHHHHHCCCEEEEEECCCCCCCEEEEEE
KGPNGESLPHKMDPYGAHNEQYPSFASVVYDQTSYQWNDAKWQQRPVTEKQKEALSFYEL
ECCCCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHEE
HAGSWKRNENGDFLTYRELAEQLIPYILDMGYTHIELMPVSEHPFYGSWGYQPIGLFSPT
CCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEECCCCC
SRFGTPDDFKYFVDQCHQVGIGVVLDWVPAHFPSDSHGLANFDGTSLFNDPDPRRGWHND
CCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
WQSFIYNYDQPHVREFLVSNALYWFEHFHIDGLRVDAVASMLYLDYSRNDGEWIPNWEGG
HHHHHCCCCCHHHHHHHHHHHHHHHHHEECCCEEHHHHHHHHEEEECCCCCCCCCCCCCC
NHNHGAIALLKWMNEEVYSHYPNAMTIAEESTAFPGVSAPTFAGGLGFGFKWNMGWMHDS
CCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCHHHH
LNYIREDPIHRKYHHDTITFPLVYAFSENFILSLSHDEVVYGKGSILDKMPGDEWQKTAN
HHHHHCCCCCHHHCCCCEEEEEEEEECCCEEEEECCCEEEECCCCCCCCCCCHHHHHHHC
LRAYMGYMYGQPGKKLNFMGAEIAQSAEWDHDGQLQWFLTQFERHSGMQSLVRDLNKLYT
HHHHHHHHHCCCCCEEEECCHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHC
TEPALYQKDCEPAGFEWRLQDEAEMSVLAHERLGDNGERILVVSNFTPAPREDFRLGMPV
CCCHHHHCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHCEECCCC
AGQYELILNSDAHFYGGSDYSVISEASTEKVESQGLAQSIVITLPPLSTVFYRLK
CCCEEEEECCCCCEECCCCCCCHHCCHHHHHHHCCCCEEEEEEECCHHHHHEECC
>Mature Secondary Structure
MQLERAAFSDPFSFLGPQYQSQTTALRVWLPGATSVKVRLSGHVEYQLLSDPRHSGIFVL
CCCCCCCCCCCHHHHCCCCCCCCEEEEEEECCCCEEEEEEECCEEEEEECCCCCCCEEEE
NESIDMTEVHYELIIDWSGTEQILDDPYQYHDISPTDAQVHTPKEMYNHLGAHLFNVVRD
ECCCCCEEEEEEEEEECCCCHHHHCCCHHCCCCCCCCCCEECHHHHHHHHHHHHHHHHHC
GKQIQGVRYLVFAPNASSASVIGDFNQWDGRRHIMQRIDNGLWALFIPEHAVGTKYKFEL
CCCCCCEEEEEECCCCCCCCEEECCHHCCCHHHHHHHHCCCEEEEEECCCCCCCEEEEEE
KGPNGESLPHKMDPYGAHNEQYPSFASVVYDQTSYQWNDAKWQQRPVTEKQKEALSFYEL
ECCCCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHEE
HAGSWKRNENGDFLTYRELAEQLIPYILDMGYTHIELMPVSEHPFYGSWGYQPIGLFSPT
CCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEECCCCC
SRFGTPDDFKYFVDQCHQVGIGVVLDWVPAHFPSDSHGLANFDGTSLFNDPDPRRGWHND
CCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
WQSFIYNYDQPHVREFLVSNALYWFEHFHIDGLRVDAVASMLYLDYSRNDGEWIPNWEGG
HHHHHCCCCCHHHHHHHHHHHHHHHHHEECCCEEHHHHHHHHEEEECCCCCCCCCCCCCC
NHNHGAIALLKWMNEEVYSHYPNAMTIAEESTAFPGVSAPTFAGGLGFGFKWNMGWMHDS
CCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCHHHH
LNYIREDPIHRKYHHDTITFPLVYAFSENFILSLSHDEVVYGKGSILDKMPGDEWQKTAN
HHHHHCCCCCHHHCCCCEEEEEEEEECCCEEEEECCCEEEECCCCCCCCCCCHHHHHHHC
LRAYMGYMYGQPGKKLNFMGAEIAQSAEWDHDGQLQWFLTQFERHSGMQSLVRDLNKLYT
HHHHHHHHHCCCCCEEEECCHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHC
TEPALYQKDCEPAGFEWRLQDEAEMSVLAHERLGDNGERILVVSNFTPAPREDFRLGMPV
CCCHHHHCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHCEECCCC
AGQYELILNSDAHFYGGSDYSVISEASTEKVESQGLAQSIVITLPPLSTVFYRLK
CCCEEEEECCCCCEECCCCCCCHHCCHHHHHHHCCCCEEEEEEECCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA