Click here to switch to the map view.

The map label for this gene is ackA2 [H]

Identifier: 59713977

GI number: 59713977

Start: 889937

End: 891133

Strand: Reverse

Name: ackA2 [H]

Synonym: VF_A0794

Alternate gene names: 59713977

Gene position: 891133-889937 (Counterclockwise)

Preceding gene: 59713980

Following gene: 59713975

Centisome position: 66.99

GC content: 41.52

Gene sequence:

>1197_bases
ATGATGAACAACGAATACGTATTAGTTATCAATTCAGGCAGCTCTTCACTTAAGTTTGCTGTAATCGATTCTGTTTCTGG
TGATGCAGTATTAAGCGGGTTAGGTGAGTGTTTTGGCCTTGAAGATGCTCGTATGAGCTGGAAGTATCAAGGACAGAAAA
CAGAAATTGCCATTGAAGGTAATGAAAACCACCATAAGATAGCTATTGGTAAATTAGTAGGTCTAACAGAAGAGTTAGGT
TTTACTGATGGTATCGTTGCTATCGGTCACCGTATCGTTCACGGCGGTGAAAAATTTACTAAGACAGTTCGTATTAATGA
AGAAGTAACAAAAGAAATTGAAAGTCTATCTGACTTAGCTCCTCTTCATAACCCAGCTGGCGCTATTGGCATTCGTGCTG
CTGTTGAAGCCTTCCCTTCTCTCCCTCAATTTGCGGTATTTGACACAGCGTTCCACCAAACTATGCCTAAGCGTGCATAC
ACTGGTGCTATTGCTAAAGAGTTATATACTGATTTTGGTGTTCGTCGTTACGGTTTCCACGGTACAAGTCACTACTTTGT
AAGCCGTGAAGCTGCAAAAATGATCAATAAACCGATTGAAGAGTCGAACTTTATTTCTGTTCACCTAGGTAATGGCGCAT
CAGTTTGTGCAATTAAAGATGGTAACAGTGTAGACACATCAATGGGCTTCACTCCGCTTTCAGGTCTAATGATGGGTACA
CGTTGTGGTGACTTAGACCCAGGTATCATTGAATACCTACTTAAGAAAGGTTGGTCACAAGAGCAAGTGTTTAACTCTCT
AAATAAAGAGTCTGGTTTCCTAGGTGTGTCCGGTCTAACAAGTGATGCTCGTGGTATCCTTGAAGCAATGGAAGAGGGAC
ACGAAGGTGCAACATTAGCATTCCAAGTGTTTACATACCGTGTTGCTAAGTACGTGGCTTCTTACCTAGCGGCATTAGAT
TCTTTAGATGGCATCATCTTCACTGGTGGTATCGGTGAGAACTCATTACCAATCCGTCGTGAGATCTTAAGCAACCTTAA
GATTCTTGGTTTTGTTGAAGATGTTGCAGGTAACGAAGGTGCTCGTTTTGGCGCTGATGGTATTATTGCTAAATCAGAAA
TGTTGAACGCTGTTGCTATGGTTATCCCAACTAACGAAGAATTCGTAATCGCACAACAATCGGTTGAACTACTGTAA

Upstream 100 bases:

>100_bases
TAAAACAAAAGTTGAATCATTAAAAAATAGTTGAATAATGGGTTGTAGCAACAAAAGGCACGACTCTAATGTGCAACCTA
CATATCTATAAAAGAGAATA

Downstream 100 bases:

>100_bases
TTTCTTCTAAAATACAGAATATTGAAAAGCGAGCTATGATGGCTCGCTTTTTTTGTTTTATATGTTGGTTGAACTATTAC
GAACGATTCGTTCTGCGTCT

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase 2 [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MMNNEYVLVINSGSSSLKFAVIDSVSGDAVLSGLGECFGLEDARMSWKYQGQKTEIAIEGNENHHKIAIGKLVGLTEELG
FTDGIVAIGHRIVHGGEKFTKTVRINEEVTKEIESLSDLAPLHNPAGAIGIRAAVEAFPSLPQFAVFDTAFHQTMPKRAY
TGAIAKELYTDFGVRRYGFHGTSHYFVSREAAKMINKPIEESNFISVHLGNGASVCAIKDGNSVDTSMGFTPLSGLMMGT
RCGDLDPGIIEYLLKKGWSQEQVFNSLNKESGFLGVSGLTSDARGILEAMEEGHEGATLAFQVFTYRVAKYVASYLAALD
SLDGIIFTGGIGENSLPIRREILSNLKILGFVEDVAGNEGARFGADGIIAKSEMLNAVAMVIPTNEEFVIAQQSVELL

Sequences:

>Translated_398_residues
MMNNEYVLVINSGSSSLKFAVIDSVSGDAVLSGLGECFGLEDARMSWKYQGQKTEIAIEGNENHHKIAIGKLVGLTEELG
FTDGIVAIGHRIVHGGEKFTKTVRINEEVTKEIESLSDLAPLHNPAGAIGIRAAVEAFPSLPQFAVFDTAFHQTMPKRAY
TGAIAKELYTDFGVRRYGFHGTSHYFVSREAAKMINKPIEESNFISVHLGNGASVCAIKDGNSVDTSMGFTPLSGLMMGT
RCGDLDPGIIEYLLKKGWSQEQVFNSLNKESGFLGVSGLTSDARGILEAMEEGHEGATLAFQVFTYRVAKYVASYLAALD
SLDGIIFTGGIGENSLPIRREILSNLKILGFVEDVAGNEGARFGADGIIAKSEMLNAVAMVIPTNEEFVIAQQSVELL
>Mature_398_residues
MMNNEYVLVINSGSSSLKFAVIDSVSGDAVLSGLGECFGLEDARMSWKYQGQKTEIAIEGNENHHKIAIGKLVGLTEELG
FTDGIVAIGHRIVHGGEKFTKTVRINEEVTKEIESLSDLAPLHNPAGAIGIRAAVEAFPSLPQFAVFDTAFHQTMPKRAY
TGAIAKELYTDFGVRRYGFHGTSHYFVSREAAKMINKPIEESNFISVHLGNGASVCAIKDGNSVDTSMGFTPLSGLMMGT
RCGDLDPGIIEYLLKKGWSQEQVFNSLNKESGFLGVSGLTSDARGILEAMEEGHEGATLAFQVFTYRVAKYVASYLAALD
SLDGIIFTGGIGENSLPIRREILSNLKILGFVEDVAGNEGARFGADGIIAKSEMLNAVAMVIPTNEEFVIAQQSVELL

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=402, Percent_Identity=50.9950248756219, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI145693184, Length=405, Percent_Identity=44.9382716049383, Blast_Score=325, Evalue=3e-90,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 42873; Mature: 42873

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNNEYVLVINSGSSSLKFAVIDSVSGDAVLSGLGECFGLEDARMSWKYQGQKTEIAIEG
CCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHCCCCCHHCCEEECCCEEEEEEEC
NENHHKIAIGKLVGLTEELGFTDGIVAIGHRIVHGGEKFTKTVRINEEVTKEIESLSDLA
CCCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHEEEEEECHHHHHHHHHHHHHC
PLHNPAGAIGIRAAVEAFPSLPQFAVFDTAFHQTMPKRAYTGAIAKELYTDFGVRRYGFH
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEECCC
GTSHYFVSREAAKMINKPIEESNFISVHLGNGASVCAIKDGNSVDTSMGFTPLSGLMMGT
CCCEEEECHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHCCC
RCGDLDPGIIEYLLKKGWSQEQVFNSLNKESGFLGVSGLTSDARGILEAMEEGHEGATLA
CCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCEEH
FQVFTYRVAKYVASYLAALDSLDGIIFTGGIGENSLPIRREILSNLKILGFVEDVAGNEG
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHCCCEEEEEHHHHCCCCC
ARFGADGIIAKSEMLNAVAMVIPTNEEFVIAQQSVELL
CCCCCCCCEEHHHHHCEEEEEEECCCCEEEECCCCCCC
>Mature Secondary Structure
MMNNEYVLVINSGSSSLKFAVIDSVSGDAVLSGLGECFGLEDARMSWKYQGQKTEIAIEG
CCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHCCCCCHHCCEEECCCEEEEEEEC
NENHHKIAIGKLVGLTEELGFTDGIVAIGHRIVHGGEKFTKTVRINEEVTKEIESLSDLA
CCCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHEEEEEECHHHHHHHHHHHHHC
PLHNPAGAIGIRAAVEAFPSLPQFAVFDTAFHQTMPKRAYTGAIAKELYTDFGVRRYGFH
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEECCC
GTSHYFVSREAAKMINKPIEESNFISVHLGNGASVCAIKDGNSVDTSMGFTPLSGLMMGT
CCCEEEECHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHCCC
RCGDLDPGIIEYLLKKGWSQEQVFNSLNKESGFLGVSGLTSDARGILEAMEEGHEGATLA
CCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCEEH
FQVFTYRVAKYVASYLAALDSLDGIIFTGGIGENSLPIRREILSNLKILGFVEDVAGNEG
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHCCCEEEEEHHHHCCCCC
ARFGADGIIAKSEMLNAVAMVIPTNEEFVIAQQSVELL
CCCCCCCCEEHHHHHCEEEEEEECCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA