Click here to switch to the map view.

The map label for this gene is mdtL [H]

Identifier: 59713953

GI number: 59713953

Start: 864938

End: 866140

Strand: Reverse

Name: mdtL [H]

Synonym: VF_A0770

Alternate gene names: 59713953

Gene position: 866140-864938 (Counterclockwise)

Preceding gene: 59713956

Following gene: 59713952

Centisome position: 65.11

GC content: 38.24

Gene sequence:

>1203_bases
ATGAATACGAATAATACAGATACATTGTCGCCAAGCCGATTAAATAAAACCCCTTTGCTTTTAGCTATGATGATCATCGC
TACGGGGCAAGTTGGTGTAAGTATCTATTTGCCTTCTTTGCCACTTATTAGCCAAGAACTGGGTATATCTTCAGCTGATG
TTCAGTTACTTGTGACTTTATTTTTGGTCGGGTTTGGTGGCTCACAGCTATTTTATGGCCCATTATCTGATGCGTTAGGA
AGACGTCCTATTTTTATATTGGGTCAGGGTGTGTATTTAGTTGGCACCTTGCTTTGCTTCACAATGACCAATAATTTTAC
GGCATTAGTCTTAGGTCGCTTATTACAAGGGTTGGGTGCTGGTAGTGCATCTGTATTAGGGCGGAGTGTTTTACGAGATA
GTTATAGTGGTTACCACCTAACTAAAGCTATGTCTTACATATCGATTACTGCATCCATTTTACCTATTCTTGCTCCTGTT
ATTGGTGGATGGGTTGCATGGCACTTTAGTTGGCAGTGGGTTTTTGGGTTTGTACTGATATATCTAATATGTATATTGAT
TATTGGTTATTTCATATTGCCTGAGACGTTACCTTACGCCCCTCAAAAGTTCAGTATTACTCAGACGTTACGAGATTACA
CTGCGTTGATTAAAAACCCTCAGATTATCTCTAGTGCAGGATTAAATTGGGTGAGTTATCTTGCAAGTTTGGTTTCCGTG
TCTCTTTTACCATTTTTACTACAAGATGGATTTGGACTAACCGCTGCAGAATATGGTCAAACCATGATTATTCCATCTGC
TGGGCTTATGATGGGAAGTTTTTTGCTTAATAGCTTAAATAAACGATGCTCGATAAATCAATTAATGAGCTTAGCGTTAG
TGATTATGACTATCTCAGGTTTATGGTTACTTTTCGCTTCAACGTCCATTTTTTCACTGGTTTTTGCGTTTACTTTACTT
GCAATAGCACAAGGGATCAGCTTTCCATTATCGATCAATTTATTACTTGCACCCCATACACATAAAGTAGGAACTATTTC
CGCGTTGTCAGGCTCAGTTCAAATGTGTCTTTCCGGTCTTTTAGGTGGCTATTTAGTAAAAAACTGGGTAACGGACCAAG
TAAATTTAGGCATATTTTATTTAGTGATAACCATAAGTATGCTATTGGTCTTATTGTGGAATAAGCAGGCACAGCCAAAA
TAA

Upstream 100 bases:

>100_bases
TTACACTCTCAATCCAATCCATAATTCGGTCCTGTTAGGAATTAATGTGATTCCTATATAGTCTATTATCATTTATATAG
GGTCAATGTAAACTAACATT

Downstream 100 bases:

>100_bases
ATGTCTTTTAAATTATAATCTTAGCGTTAATTTAGTGAAGGATGTTGCTATTTCATCTTTAGGTTCTTTATTCTTGGAAT
TAATAACTAAATAAATTGTC

Product: bicyclomycin resistance protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MNTNNTDTLSPSRLNKTPLLLAMMIIATGQVGVSIYLPSLPLISQELGISSADVQLLVTLFLVGFGGSQLFYGPLSDALG
RRPIFILGQGVYLVGTLLCFTMTNNFTALVLGRLLQGLGAGSASVLGRSVLRDSYSGYHLTKAMSYISITASILPILAPV
IGGWVAWHFSWQWVFGFVLIYLICILIIGYFILPETLPYAPQKFSITQTLRDYTALIKNPQIISSAGLNWVSYLASLVSV
SLLPFLLQDGFGLTAAEYGQTMIIPSAGLMMGSFLLNSLNKRCSINQLMSLALVIMTISGLWLLFASTSIFSLVFAFTLL
AIAQGISFPLSINLLLAPHTHKVGTISALSGSVQMCLSGLLGGYLVKNWVTDQVNLGIFYLVITISMLLVLLWNKQAQPK

Sequences:

>Translated_400_residues
MNTNNTDTLSPSRLNKTPLLLAMMIIATGQVGVSIYLPSLPLISQELGISSADVQLLVTLFLVGFGGSQLFYGPLSDALG
RRPIFILGQGVYLVGTLLCFTMTNNFTALVLGRLLQGLGAGSASVLGRSVLRDSYSGYHLTKAMSYISITASILPILAPV
IGGWVAWHFSWQWVFGFVLIYLICILIIGYFILPETLPYAPQKFSITQTLRDYTALIKNPQIISSAGLNWVSYLASLVSV
SLLPFLLQDGFGLTAAEYGQTMIIPSAGLMMGSFLLNSLNKRCSINQLMSLALVIMTISGLWLLFASTSIFSLVFAFTLL
AIAQGISFPLSINLLLAPHTHKVGTISALSGSVQMCLSGLLGGYLVKNWVTDQVNLGIFYLVITISMLLVLLWNKQAQPK
>Mature_400_residues
MNTNNTDTLSPSRLNKTPLLLAMMIIATGQVGVSIYLPSLPLISQELGISSADVQLLVTLFLVGFGGSQLFYGPLSDALG
RRPIFILGQGVYLVGTLLCFTMTNNFTALVLGRLLQGLGAGSASVLGRSVLRDSYSGYHLTKAMSYISITASILPILAPV
IGGWVAWHFSWQWVFGFVLIYLICILIIGYFILPETLPYAPQKFSITQTLRDYTALIKNPQIISSAGLNWVSYLASLVSV
SLLPFLLQDGFGLTAAEYGQTMIIPSAGLMMGSFLLNSLNKRCSINQLMSLALVIMTISGLWLLFASTSIFSLVFAFTLL
AIAQGISFPLSINLLLAPHTHKVGTISALSGSVQMCLSGLLGGYLVKNWVTDQVNLGIFYLVITISMLLVLLWNKQAQPK

Specific function: Confers resistance to chloramphenicol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtL (TC 2.A.1.2.22) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790146, Length=334, Percent_Identity=29.0419161676647, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1788509, Length=350, Percent_Identity=24.8571428571429, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI48994889, Length=348, Percent_Identity=26.1494252873563, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI87082312, Length=344, Percent_Identity=28.7790697674419, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1787065, Length=359, Percent_Identity=26.4623955431755, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790794, Length=218, Percent_Identity=27.0642201834862, Blast_Score=75, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319657, Length=165, Percent_Identity=30.9090909090909, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43278; Mature: 43278

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTNNTDTLSPSRLNKTPLLLAMMIIATGQVGVSIYLPSLPLISQELGISSADVQLLVTL
CCCCCCCCCCCHHCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHHH
FLVGFGGSQLFYGPLSDALGRRPIFILGQGVYLVGTLLCFTMTNNFTALVLGRLLQGLGA
HHHHCCCCCEEECCHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
GSASVLGRSVLRDSYSGYHLTKAMSYISITASILPILAPVIGGWVAWHFSWQWVFGFVLI
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLICILIIGYFILPETLPYAPQKFSITQTLRDYTALIKNPQIISSAGLNWVSYLASLVSV
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHH
SLLPFLLQDGFGLTAAEYGQTMIIPSAGLMMGSFLLNSLNKRCSINQLMSLALVIMTISG
HHHHHHHHCCCCCCHHHCCCEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LWLLFASTSIFSLVFAFTLLAIAQGISFPLSINLLLAPHTHKVGTISALSGSVQMCLSGL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
LGGYLVKNWVTDQVNLGIFYLVITISMLLVLLWNKQAQPK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNTNNTDTLSPSRLNKTPLLLAMMIIATGQVGVSIYLPSLPLISQELGISSADVQLLVTL
CCCCCCCCCCCHHCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHHH
FLVGFGGSQLFYGPLSDALGRRPIFILGQGVYLVGTLLCFTMTNNFTALVLGRLLQGLGA
HHHHCCCCCEEECCHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
GSASVLGRSVLRDSYSGYHLTKAMSYISITASILPILAPVIGGWVAWHFSWQWVFGFVLI
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLICILIIGYFILPETLPYAPQKFSITQTLRDYTALIKNPQIISSAGLNWVSYLASLVSV
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHH
SLLPFLLQDGFGLTAAEYGQTMIIPSAGLMMGSFLLNSLNKRCSINQLMSLALVIMTISG
HHHHHHHHCCCCCCHHHCCCEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LWLLFASTSIFSLVFAFTLLAIAQGISFPLSINLLLAPHTHKVGTISALSGSVQMCLSGL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
LGGYLVKNWVTDQVNLGIFYLVITISMLLVLLWNKQAQPK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA