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The map label for this gene is htrB1 [H]

Identifier: 59713870

GI number: 59713870

Start: 760252

End: 761187

Strand: Reverse

Name: htrB1 [H]

Synonym: VF_A0687

Alternate gene names: 59713870

Gene position: 761187-760252 (Counterclockwise)

Preceding gene: 59713871

Following gene: 59713867

Centisome position: 57.22

GC content: 38.25

Gene sequence:

>936_bases
ATGAAAACAGTTCCAAGTCCTAAATTTACATGGGCGCTGTTGTCTCCAAAGTACTGGCTTGTATGGCTATCTTTTTCTTT
TCTTGCTGTATTGGTTAATGTACTTCCGTTCGTCGTCCTTAAAAGAATAGGGAATGGAATCGGATGTTTATCTAAATTTT
GCTTAAAAAAGCGTGCGAACGTTGCTCGAAAAAATCTAGCATTATGTTTTCCTCATTATTCACAAACAGAAATTGAGCGA
TTAGTGAATGAAAACTTTAAGAATACTGGGTTAGCATTAATTGAAACCGGTATGGCTTGGTTTTGGCCTGATTGGCGTGT
TAAGCGTCATATATCCATTATTGGCCAAGAGAAAATGTTAGAAAAAGAGCAAGAAGGTAAAGGCGTATTAGTGCTGTGTT
GTCATTTTTTAAATCTTGAAATGACAGCTCGAATTTTTAGTCTTTTTGCTCCTGGTTATGGCGTGTATCGTGCTAATAGC
AATCCTGTGTATGAATTTATTCAACATCGCGGACGTACGCGTAAAGGTCATAAGATGATTGACCGTCATGATGTTAAAAG
CATGATTAAGGTTCTTAAGTCTGGAAATCGTTTGTGGTATTTGCCTGATCATGACTATGGCCGCCGTAACTCAGTGTTTG
TTCCATTTTTTGCTGTTCAAGAAGCGGCAACAACTACGGGTTCCAGTTTCTTAATTGATATGACAAAGTGTGCTGTTATG
TCGGGTACCAGTATACGAACTGACAATCACTATACTTTAGAAATAGGGGATGACATTAGTGATGATATTCCACGCCGTGA
TGCTGTTACAGCTGCAAATGTACTAAATAGAGAGATTGAACGTTTAATTGAGAAAGAACCTTCGCAATGGATGTGGCTAC
ATAAACGCTTCAAAACGTTACCAGAAGACTCAGAAGAGATGAATCGATACGCATAA

Upstream 100 bases:

>100_bases
TAATTTGATCAAACTTGATTGAGCTTATTAAACTTTTCAACTAAAATTTCACTTTAATTATATTTCTGAGACAGGTGTCT
TAGTTTTGATGGGTGAAAAA

Downstream 100 bases:

>100_bases
AAGTTGTATTTAATTCAAAACTATTTCTCAATATAAAAAAGCCTCGCTGAATTAGCGAGGCTTTTGTTTATAAAGCGTTT
GTTATTAAGCTAACAATTCT

Product: lipid A biosynthesis lauroyl acyltransferase

Products: KDO2-(lauroyl)-lipid IVA [C]

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKTVPSPKFTWALLSPKYWLVWLSFSFLAVLVNVLPFVVLKRIGNGIGCLSKFCLKKRANVARKNLALCFPHYSQTEIER
LVNENFKNTGLALIETGMAWFWPDWRVKRHISIIGQEKMLEKEQEGKGVLVLCCHFLNLEMTARIFSLFAPGYGVYRANS
NPVYEFIQHRGRTRKGHKMIDRHDVKSMIKVLKSGNRLWYLPDHDYGRRNSVFVPFFAVQEAATTTGSSFLIDMTKCAVM
SGTSIRTDNHYTLEIGDDISDDIPRRDAVTAANVLNREIERLIEKEPSQWMWLHKRFKTLPEDSEEMNRYA

Sequences:

>Translated_311_residues
MKTVPSPKFTWALLSPKYWLVWLSFSFLAVLVNVLPFVVLKRIGNGIGCLSKFCLKKRANVARKNLALCFPHYSQTEIER
LVNENFKNTGLALIETGMAWFWPDWRVKRHISIIGQEKMLEKEQEGKGVLVLCCHFLNLEMTARIFSLFAPGYGVYRANS
NPVYEFIQHRGRTRKGHKMIDRHDVKSMIKVLKSGNRLWYLPDHDYGRRNSVFVPFFAVQEAATTTGSSFLIDMTKCAVM
SGTSIRTDNHYTLEIGDDISDDIPRRDAVTAANVLNREIERLIEKEPSQWMWLHKRFKTLPEDSEEMNRYA
>Mature_311_residues
MKTVPSPKFTWALLSPKYWLVWLSFSFLAVLVNVLPFVVLKRIGNGIGCLSKFCLKKRANVARKNLALCFPHYSQTEIER
LVNENFKNTGLALIETGMAWFWPDWRVKRHISIIGQEKMLEKEQEGKGVLVLCCHFLNLEMTARIFSLFAPGYGVYRANS
NPVYEFIQHRGRTRKGHKMIDRHDVKSMIKVLKSGNRLWYLPDHDYGRRNSVFVPFFAVQEAATTTGSSFLIDMTKCAVM
SGTSIRTDNHYTLEIGDDISDDIPRRDAVTAANVLNREIERLIEKEPSQWMWLHKRFKTLPEDSEEMNRYA

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=297, Percent_Identity=45.7912457912458, Blast_Score=272, Evalue=2e-74,
Organism=Escherichia coli, GI87082094, Length=299, Percent_Identity=39.7993311036789, Blast_Score=247, Evalue=6e-67,
Organism=Escherichia coli, GI1788161, Length=299, Percent_Identity=27.4247491638796, Blast_Score=104, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 36026; Mature: 36026

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVPSPKFTWALLSPKYWLVWLSFSFLAVLVNVLPFVVLKRIGNGIGCLSKFCLKKRAN
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VARKNLALCFPHYSQTEIERLVNENFKNTGLALIETGMAWFWPDWRVKRHISIIGQEKML
HHHHCCEEEECCCCHHHHHHHHHCCCCCCCHHHEECCCHHCCCCHHHHHHHHHHHHHHHH
EKEQEGKGVLVLCCHFLNLEMTARIFSLFAPGYGVYRANSNPVYEFIQHRGRTRKGHKMI
HHHHCCCCEEEEHHHHHCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCHHHHH
DRHDVKSMIKVLKSGNRLWYLPDHDYGRRNSVFVPFFAVQEAATTTGSSFLIDMTKCAVM
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEHHHHHHHHHCCCCHHEEEHHHHHHC
SGTSIRTDNHYTLEIGDDISDDIPRRDAVTAANVLNREIERLIEKEPSQWMWLHKRFKTL
CCCEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
PEDSEEMNRYA
CCCHHHHHCCC
>Mature Secondary Structure
MKTVPSPKFTWALLSPKYWLVWLSFSFLAVLVNVLPFVVLKRIGNGIGCLSKFCLKKRAN
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VARKNLALCFPHYSQTEIERLVNENFKNTGLALIETGMAWFWPDWRVKRHISIIGQEKML
HHHHCCEEEECCCCHHHHHHHHHCCCCCCCHHHEECCCHHCCCCHHHHHHHHHHHHHHHH
EKEQEGKGVLVLCCHFLNLEMTARIFSLFAPGYGVYRANSNPVYEFIQHRGRTRKGHKMI
HHHHCCCCEEEEHHHHHCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCHHHHH
DRHDVKSMIKVLKSGNRLWYLPDHDYGRRNSVFVPFFAVQEAATTTGSSFLIDMTKCAVM
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEHHHHHHHHHCCCCHHEEEHHHHHHC
SGTSIRTDNHYTLEIGDDISDDIPRRDAVTAANVLNREIERLIEKEPSQWMWLHKRFKTL
CCCEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
PEDSEEMNRYA
CCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO2-lipid IVA; lauroyl-ACP [C]

Specific reaction: KDO2-lipid IVA + lauroyl-ACP = KDO2-(lauroyl)-lipid IVA + [acyl carrier protein] [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]