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The map label for this gene is pykA [H]

Identifier: 59713677

GI number: 59713677

Start: 568497

End: 569942

Strand: Reverse

Name: pykA [H]

Synonym: VF_A0494

Alternate gene names: 59713677

Gene position: 569942-568497 (Counterclockwise)

Preceding gene: 59713678

Following gene: 59713675

Centisome position: 42.84

GC content: 43.85

Gene sequence:

>1446_bases
ATGTCTAACAGACTACGTAGAACAAAAATCGTTACCACTCTTGGCCCTGCAACAGATCGTGATAATAACCTAGAAAAAAT
CATTGCCGCTGGTGCAAACGTTGTTCGTATGAACTTCTCTCACGGTAGCCCAGAAGATCACATTGAGCGTACACGCAAAG
TTCGTGAAATCGCAGCAAAACTTGGTACTCACGTTGCTATTCTAGGTGACTTACAAGGTCCTAAAATCCGTGTTTCTACG
TTTAAAGAAGGTAAAATCCAATTAGCTATTGGTGATAAATTCACGCTAGATAGCGATTTACCTAAAGGTGAAGGTAATCA
AGACTCTGTTGGCCTTGATTACAAAGAACTGCCTCAAGATGTAACTACTGGCGACGTTCTTCTTCTTGATGACGGCCGTG
TACAACTTCGTGTAACTGCTGTTGAAGGCAACAAAGTACACACAGAAGTAACAGTTGCTGGTCCTCTATCAAACAATAAA
GGCATCAACAAACAAGGTGGTGGCCTTTCTGCTGACGCACTAACTGAAAAAGACAAAGCTGACATCATTACAGCTGCTGC
AATGAAAGTTGATTACCTAGCGGTTTCTTTCCCACGTAACGGCGAAGACATGAACTACGCTCGTCGTCTAGCTCGTGATG
CAGGTCTTGAAGCGCAATTAGTTGCTAAAGTTGAACGTGCTGAAACGGTAGCGTCTGAAGAAGCACTGGATGACATCATC
CTTGCATCTGATGTTGTTATGGTTGCACGTGGTGACCTAGGTGTTGAAATTGGCGATCCAGAGCTAGTTGGTGTTCAAAA
GCAACTAATCCGTCGTGCTCGTAGTCTTAACCGTACGGTTATTACTGCAACACAAATGATGGAATCAATGATTTCTAGCC
CAATGCCTACTCGTGCTGAAGTAATGGACGTTGCTAACGCCGTTCTTGATGGTACAGATGCGGTAATGCTTTCTGCTGAA
ACAGCGGCTGGTGACTTCCCTGTTGAAACAGTAACTTCAATGGCTGGCGTTTGTATGGGTGCTGAAAAAGTACCTACAGT
TAACGTATCTAACCACCGTTTAGACCGTCGCTTCACGACACCAGAAGAAACAATTGCAATGTCTACTATGTATGCAGCAA
ACCACATGGAAGGCATCACAGCGATGATCACACTTACAGAGTCTGGTCGTACTCCACTTATGATGTCTCGTCTAAGTTCT
GGTCTACCTATCTTTGCTCTATCTCGCAACGAAGGTACGCTAAACCGTGCAGCGCTTTACCGTGGTGTGACTCCAGTATT
CTTTGATGAGAAAGCCGATTCAGGTCTTCCATCAACTTATGCTGCAATCAACACGCTAAAATCTAAAGGTTTACTATCAA
CTGGCGACCAAGTAATCATCACTCAAGGTGACGTTATGGACATCGTTGGTTCAACAAACTGTATGCGTATTATTACTGTT
GAGTAA

Upstream 100 bases:

>100_bases
AATTTTTTACATTTACCCTGTTTGAGTCATGGTATTGTCACTATTGCCGATATATGTTTAAGCATAGGAAAAATAAAACA
ACATCCACTTTGGAGATTAT

Downstream 100 bases:

>100_bases
TTAAACATCGCATAAAATAAAAGCCCCGCTGTTAAATCAACATCGGGGCTTTTTTATTTCATCCAAATAACGATATTTGG
CTATGATTTCCAACCATCAT

Product: pyruvate kinase II

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSNRLRRTKIVTTLGPATDRDNNLEKIIAAGANVVRMNFSHGSPEDHIERTRKVREIAAKLGTHVAILGDLQGPKIRVST
FKEGKIQLAIGDKFTLDSDLPKGEGNQDSVGLDYKELPQDVTTGDVLLLDDGRVQLRVTAVEGNKVHTEVTVAGPLSNNK
GINKQGGGLSADALTEKDKADIITAAAMKVDYLAVSFPRNGEDMNYARRLARDAGLEAQLVAKVERAETVASEEALDDII
LASDVVMVARGDLGVEIGDPELVGVQKQLIRRARSLNRTVITATQMMESMISSPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGDFPVETVTSMAGVCMGAEKVPTVNVSNHRLDRRFTTPEETIAMSTMYAANHMEGITAMITLTESGRTPLMMSRLSS
GLPIFALSRNEGTLNRAALYRGVTPVFFDEKADSGLPSTYAAINTLKSKGLLSTGDQVIITQGDVMDIVGSTNCMRIITV
E

Sequences:

>Translated_481_residues
MSNRLRRTKIVTTLGPATDRDNNLEKIIAAGANVVRMNFSHGSPEDHIERTRKVREIAAKLGTHVAILGDLQGPKIRVST
FKEGKIQLAIGDKFTLDSDLPKGEGNQDSVGLDYKELPQDVTTGDVLLLDDGRVQLRVTAVEGNKVHTEVTVAGPLSNNK
GINKQGGGLSADALTEKDKADIITAAAMKVDYLAVSFPRNGEDMNYARRLARDAGLEAQLVAKVERAETVASEEALDDII
LASDVVMVARGDLGVEIGDPELVGVQKQLIRRARSLNRTVITATQMMESMISSPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGDFPVETVTSMAGVCMGAEKVPTVNVSNHRLDRRFTTPEETIAMSTMYAANHMEGITAMITLTESGRTPLMMSRLSS
GLPIFALSRNEGTLNRAALYRGVTPVFFDEKADSGLPSTYAAINTLKSKGLLSTGDQVIITQGDVMDIVGSTNCMRIITV
E
>Mature_480_residues
SNRLRRTKIVTTLGPATDRDNNLEKIIAAGANVVRMNFSHGSPEDHIERTRKVREIAAKLGTHVAILGDLQGPKIRVSTF
KEGKIQLAIGDKFTLDSDLPKGEGNQDSVGLDYKELPQDVTTGDVLLLDDGRVQLRVTAVEGNKVHTEVTVAGPLSNNKG
INKQGGGLSADALTEKDKADIITAAAMKVDYLAVSFPRNGEDMNYARRLARDAGLEAQLVAKVERAETVASEEALDDIIL
ASDVVMVARGDLGVEIGDPELVGVQKQLIRRARSLNRTVITATQMMESMISSPMPTRAEVMDVANAVLDGTDAVMLSAET
AAGDFPVETVTSMAGVCMGAEKVPTVNVSNHRLDRRFTTPEETIAMSTMYAANHMEGITAMITLTESGRTPLMMSRLSSG
LPIFALSRNEGTLNRAALYRGVTPVFFDEKADSGLPSTYAAINTLKSKGLLSTGDQVIITQGDVMDIVGSTNCMRIITVE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=499, Percent_Identity=35.4709418837675, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI33286420, Length=499, Percent_Identity=35.4709418837675, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI33286418, Length=495, Percent_Identity=35.1515151515151, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI10835121, Length=493, Percent_Identity=34.2799188640974, Blast_Score=241, Evalue=2e-63,
Organism=Homo sapiens, GI32967597, Length=493, Percent_Identity=34.2799188640974, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=33.448275862069, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=33.448275862069, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=31.3559322033898, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=31.3559322033898, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=30.6976744186047, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1788160, Length=481, Percent_Identity=77.1309771309771, Blast_Score=748, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=475, Percent_Identity=37.4736842105263, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=35.4508196721311, Blast_Score=265, Evalue=5e-71,
Organism=Caenorhabditis elegans, GI71984413, Length=494, Percent_Identity=35.4251012145749, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17506831, Length=494, Percent_Identity=35.4251012145749, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI71984406, Length=494, Percent_Identity=35.4251012145749, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17506829, Length=494, Percent_Identity=35.4251012145749, Blast_Score=242, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6319279, Length=491, Percent_Identity=35.030549898167, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6324923, Length=492, Percent_Identity=32.7235772357724, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24648964, Length=497, Percent_Identity=36.0160965794769, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI28571814, Length=497, Percent_Identity=36.0160965794769, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24648966, Length=421, Percent_Identity=35.8669833729216, Blast_Score=241, Evalue=9e-64,
Organism=Drosophila melanogaster, GI24581235, Length=499, Percent_Identity=30.4609218436874, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24646914, Length=274, Percent_Identity=34.3065693430657, Blast_Score=145, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51631; Mature: 51499

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNRLRRTKIVTTLGPATDRDNNLEKIIAAGANVVRMNFSHGSPEDHIERTRKVREIAAK
CCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
LGTHVAILGDLQGPKIRVSTFKEGKIQLAIGDKFTLDSDLPKGEGNQDSVGLDYKELPQD
HCCEEEEEECCCCCEEEEEEECCCEEEEEECCCEEECCCCCCCCCCCCCCCCCHHHCCCC
VTTGDVLLLDDGRVQLRVTAVEGNKVHTEVTVAGPLSNNKGINKQGGGLSADALTEKDKA
CCCCCEEEEECCEEEEEEEEECCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCHH
DIITAAAMKVDYLAVSFPRNGEDMNYARRLARDAGLEAQLVAKVERAETVASEEALDDII
HEEEHHHEEEEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LASDVVMVARGDLGVEIGDPELVGVQKQLIRRARSLNRTVITATQMMESMISSPMPTRAE
HHCCEEEEEECCCCEEECCCCEECHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGDFPVETVTSMAGVCMGAEKVPTVNVSNHRLDRRFTT
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCC
PEETIAMSTMYAANHMEGITAMITLTESGRTPLMMSRLSSGLPIFALSRNEGTLNRAALY
CHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHH
RGVTPVFFDEKADSGLPSTYAAINTLKSKGLLSTGDQVIITQGDVMDIVGSTNCMRIITV
HCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCEEEECCCCCEEEEEEE
E
C
>Mature Secondary Structure 
SNRLRRTKIVTTLGPATDRDNNLEKIIAAGANVVRMNFSHGSPEDHIERTRKVREIAAK
CCCCCEEEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
LGTHVAILGDLQGPKIRVSTFKEGKIQLAIGDKFTLDSDLPKGEGNQDSVGLDYKELPQD
HCCEEEEEECCCCCEEEEEEECCCEEEEEECCCEEECCCCCCCCCCCCCCCCCHHHCCCC
VTTGDVLLLDDGRVQLRVTAVEGNKVHTEVTVAGPLSNNKGINKQGGGLSADALTEKDKA
CCCCCEEEEECCEEEEEEEEECCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCHH
DIITAAAMKVDYLAVSFPRNGEDMNYARRLARDAGLEAQLVAKVERAETVASEEALDDII
HEEEHHHEEEEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LASDVVMVARGDLGVEIGDPELVGVQKQLIRRARSLNRTVITATQMMESMISSPMPTRAE
HHCCEEEEEECCCCEEECCCCEECHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGDFPVETVTSMAGVCMGAEKVPTVNVSNHRLDRRFTT
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCC
PEETIAMSTMYAANHMEGITAMITLTESGRTPLMMSRLSSGLPIFALSRNEGTLNRAALY
CHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHH
RGVTPVFFDEKADSGLPSTYAAINTLKSKGLLSTGDQVIITQGDVMDIVGSTNCMRIITV
HCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCEEEECCCCCEEEEEEE
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]