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The map label for this gene is pepT [H]

Identifier: 59713436

GI number: 59713436

Start: 284061

End: 285290

Strand: Reverse

Name: pepT [H]

Synonym: VF_A0253

Alternate gene names: 59713436

Gene position: 285290-284061 (Counterclockwise)

Preceding gene: 59713437

Following gene: 59713433

Centisome position: 21.45

GC content: 40.73

Gene sequence:

>1230_bases
ATGAAAGACCTTGTAGAACGCTTTCTTTCTTATGTGAGTATTGATACTCAATCCAATCCTTCTGCACCACAATGTCCAAG
CACTGAAAAACAATTCAATTTGGCTAACCAACTAGTTTCTGAATTAAATGAATTAGAACTTTCTGATGTCTCTATTGATG
AAAATGGTTATGTCATGGGGCGTTTGCCTTCAAACGTAGACTATGATGTGCCTGCAATTGGCTTTATTGCTCACATGGAC
ACAGCCCCTGATGCATCAGGTGAAAATGTAAAACCACAAATCATCAATAATTATCAAGGCGACATAATAACCCTTGGCAC
TAGTGGTGAAGAGTTAACACCAAGCCAGTTCCCTGACTTACTCAACTTAATCGGTCACGATTTGATCACCACAGATGGTA
CGACTTTACTTGGTGCTGATAATAAAGCTGGCATTGCCGAAATCCTAACTGCCATTGCTGTATTAAAAGCGAACCCAGAG
ATCCCACACGGTGATATTTGTGTCGGCTTTACTCCTGATGAAGAGATTGGACGTGGCGCAAACCTATTTGATGTTAAGAA
ATTCAATGCAAAGTGGGCTTACACTATCGATGGTGGCCCTGTGGGTGAATTAGAATATGAAAACTTTAATGCCACAAGTG
CGGATGTCATTTGTCATGGTGTGAATGTTCACCCGGGTACGGCTAAAGGTAAGATGGTTAACTCCATGAATATCGCGGCT
CAATTTCAAATGATGATGCCAGCAGATGAAACACCAGAAGGCACCGAAGGCTATGAAGGTTTCTATCATTTAAAATCAAT
GAACGCAGGCGTAGCCAAAACCGAACTTGGTTATATTGTTCGTGATTTTAGCCGTGAAGGTATGGCTGAGCGTAAAGCCT
TTATGCAGCAAAAAGTGAATGAATTAAATGCAAAATTAGAGAAAGGTCGTGTTGAATTAGTCTTAACCGATAGCTACTTT
AATATGCGTGAAATGGTAGAACCTCACCCACACGTGATTGAACTAGCTAAAGAAGCAATGACAGCGTGTGACATTCAACC
TGATATCAAGCCTATTCGTGGCGGTACGGATGGCGCTCGTTTATCTTTTATGGGTTTACCTTGCCCTAATATCTTTACAG
GTGGTTATAACTTCCACGGTATTCATGAATTCATTACGATTAATGGCATGAAACAAGCGGTTGATGTGATTGTGAAAATT
GCAGAGTTGAACGCTAGCAACAATAAGTAA

Upstream 100 bases:

>100_bases
GCTAACCACTTTGTTTTTTATGACCCATCGCAAACTTTAAAATACCTTTTTGATCAATACTTTCACTCAATTTTGTTTTC
AATTACATAGAGTGAATATC

Downstream 100 bases:

>100_bases
TTCATAACACTACTTCTAAACATAAAAAATGCCCGATGATTACTCAAATCATCGGGCATTTTATTTTACGTTATTACATT
ATTTATACACAGACTTCAGG

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MKDLVERFLSYVSIDTQSNPSAPQCPSTEKQFNLANQLVSELNELELSDVSIDENGYVMGRLPSNVDYDVPAIGFIAHMD
TAPDASGENVKPQIINNYQGDIITLGTSGEELTPSQFPDLLNLIGHDLITTDGTTLLGADNKAGIAEILTAIAVLKANPE
IPHGDICVGFTPDEEIGRGANLFDVKKFNAKWAYTIDGGPVGELEYENFNATSADVICHGVNVHPGTAKGKMVNSMNIAA
QFQMMMPADETPEGTEGYEGFYHLKSMNAGVAKTELGYIVRDFSREGMAERKAFMQQKVNELNAKLEKGRVELVLTDSYF
NMREMVEPHPHVIELAKEAMTACDIQPDIKPIRGGTDGARLSFMGLPCPNIFTGGYNFHGIHEFITINGMKQAVDVIVKI
AELNASNNK

Sequences:

>Translated_409_residues
MKDLVERFLSYVSIDTQSNPSAPQCPSTEKQFNLANQLVSELNELELSDVSIDENGYVMGRLPSNVDYDVPAIGFIAHMD
TAPDASGENVKPQIINNYQGDIITLGTSGEELTPSQFPDLLNLIGHDLITTDGTTLLGADNKAGIAEILTAIAVLKANPE
IPHGDICVGFTPDEEIGRGANLFDVKKFNAKWAYTIDGGPVGELEYENFNATSADVICHGVNVHPGTAKGKMVNSMNIAA
QFQMMMPADETPEGTEGYEGFYHLKSMNAGVAKTELGYIVRDFSREGMAERKAFMQQKVNELNAKLEKGRVELVLTDSYF
NMREMVEPHPHVIELAKEAMTACDIQPDIKPIRGGTDGARLSFMGLPCPNIFTGGYNFHGIHEFITINGMKQAVDVIVKI
AELNASNNK
>Mature_409_residues
MKDLVERFLSYVSIDTQSNPSAPQCPSTEKQFNLANQLVSELNELELSDVSIDENGYVMGRLPSNVDYDVPAIGFIAHMD
TAPDASGENVKPQIINNYQGDIITLGTSGEELTPSQFPDLLNLIGHDLITTDGTTLLGADNKAGIAEILTAIAVLKANPE
IPHGDICVGFTPDEEIGRGANLFDVKKFNAKWAYTIDGGPVGELEYENFNATSADVICHGVNVHPGTAKGKMVNSMNIAA
QFQMMMPADETPEGTEGYEGFYHLKSMNAGVAKTELGYIVRDFSREGMAERKAFMQQKVNELNAKLEKGRVELVLTDSYF
NMREMVEPHPHVIELAKEAMTACDIQPDIKPIRGGTDGARLSFMGLPCPNIFTGGYNFHGIHEFITINGMKQAVDVIVKI
AELNASNNK

Specific function: Cleaves the N-terminal amino acid of tripeptides [H]

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family [H]

Homologues:

Organism=Escherichia coli, GI1787372, Length=413, Percent_Identity=58.1113801452784, Blast_Score=498, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.4.11.4 [H]

Molecular weight: Translated: 44608; Mature: 44608

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLVERFLSYVSIDTQSNPSAPQCPSTEKQFNLANQLVSELNELELSDVSIDENGYVMG
CHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEE
RLPSNVDYDVPAIGFIAHMDTAPDASGENVKPQIINNYQGDIITLGTSGEELTPSQFPDL
ECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHCCCCCCEEEEECCCCCCCHHHHHHH
LNLIGHDLITTDGTTLLGADNKAGIAEILTAIAVLKANPEIPHGDICVGFTPDEEIGRGA
HHHHCCCEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHCCCC
NLFDVKKFNAKWAYTIDGGPVGELEYENFNATSADVICHGVNVHPGTAKGKMVNSMNIAA
CEEEEEECCCEEEEEECCCCCCCEEECCCCCCCCCEEEECCCCCCCCCCCCEEECEEHEE
QFQMMMPADETPEGTEGYEGFYHLKSMNAGVAKTELGYIVRDFSREGMAERKAFMQQKVN
EEEEECCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ELNAKLEKGRVELVLTDSYFNMREMVEPHPHVIELAKEAMTACDIQPDIKPIRGGTDGAR
HHHHHHHCCEEEEEEECCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCCE
LSFMGLPCPNIFTGGYNFHGIHEFITINGMKQAVDVIVKIAELNASNNK
EEEECCCCCCCEECCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKDLVERFLSYVSIDTQSNPSAPQCPSTEKQFNLANQLVSELNELELSDVSIDENGYVMG
CHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEE
RLPSNVDYDVPAIGFIAHMDTAPDASGENVKPQIINNYQGDIITLGTSGEELTPSQFPDL
ECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHCCCCCCEEEEECCCCCCCHHHHHHH
LNLIGHDLITTDGTTLLGADNKAGIAEILTAIAVLKANPEIPHGDICVGFTPDEEIGRGA
HHHHCCCEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHCCCC
NLFDVKKFNAKWAYTIDGGPVGELEYENFNATSADVICHGVNVHPGTAKGKMVNSMNIAA
CEEEEEECCCEEEEEECCCCCCCEEECCCCCCCCCEEEECCCCCCCCCCCCEEECEEHEE
QFQMMMPADETPEGTEGYEGFYHLKSMNAGVAKTELGYIVRDFSREGMAERKAFMQQKVN
EEEEECCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ELNAKLEKGRVELVLTDSYFNMREMVEPHPHVIELAKEAMTACDIQPDIKPIRGGTDGAR
HHHHHHHCCEEEEEEECCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCCE
LSFMGLPCPNIFTGGYNFHGIHEFITINGMKQAVDVIVKIAELNASNNK
EEEECCCCCCCEECCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA