Click here to switch to the map view.

The map label for this gene is fadH [C]

Identifier: 59713305

GI number: 59713305

Start: 137700

End: 138776

Strand: Reverse

Name: fadH [C]

Synonym: VF_A0122

Alternate gene names: 59713305

Gene position: 138776-137700 (Counterclockwise)

Preceding gene: 59713309

Following gene: 59713302

Centisome position: 10.43

GC content: 39.65

Gene sequence:

>1077_bases
ATGACACTTAAAAACCGCTTCATGAGAAGTGCAACGTGGGAAAATATGGCAACAGAAGACGGCCATATGACAGACAAGTT
ATACGATATCTACGAAGAGCTCGCTAAAGGTGAAGTTGGCTTAATTGTTACTGGCTATGCAAACATTGTTGAAGAAGAAA
AACCCAACGCTGGCATGATGGGTATGTATAACGATTCTTTCATTGAAGAGTACAAAAAGCTGACTGAACTTGTACACCTA
AATGATTCAAAAATTGTTATGCAGCTTGCTTATGGTGGCACTAAAACGACTTATAATGTTGGTGAACGTGTTATTTTTGC
TCCAAGTGATGTGTGTGAACGTGGTACACAAACACAAGGCAAAGCGATGACAAAAGACGAAATCGACTACATTGTTGATG
CGTTTGCCAAAGCAAGCCTAAGAGCAAAAGAATCTGGTTTTGATGGTGTTGAAATCCATGCTGCTCACACCTATTTAATC
AACCAATTTTTAAGCCCTTACTATAACCGTCGTGAAGATGAATATGGCGGTAGCTTAGATAATCGTATGCGATTCTTGTT
AGAGATCTACGCAGAGATCAGAAAACAAGTGGGTGACGATTACCCTATCCTAGTAAAACTGACGGCTTCTGAGTTCTTTG
AAGGTGGTTTAACGTTCGATGAGACTCGAATCATCTGTAAAAAACTGGAAGAGATCGGTGTTGATGGGATCATCATTTCA
GGCAACATTCACGGTAATGCAAGCACACTTGTTGGTGAATCTTTTGATGGCTATACGCTTCAAGAAGAAGGTTATTTCCA
TGAATATGGCAATGTGATCAGCCAAGATGTTAATGTTCCTGTGATTACCGTTGGTGGTTTAAACGACATTAATGCTATTG
AAAAGATTGCTGAAGAAACGAATATCCAATACTTTGCTTTATCACGTCCTTTACTTGCAGAACCTCAGCTAATTAAACGT
TGGAAAGAAGGCAATAGAGCCGAAGTCGATTGCGAGCGTTGTTCTAAGTGCCGAACCAAACGTGGCAACTTCTGTGTGGT
TTATAAAAAAAGAAAACCAAAGAAAGAATGCGCTTAA

Upstream 100 bases:

>100_bases
TTTTTTGCTTTATATTGACGCTAAAATCGCCCCATAAAATAAAACCAGTAATAGAGGAACACACTTGAGCACTTTGTTTA
CCGAAACCCGCATTGGCAAC

Downstream 100 bases:

>100_bases
TCCATACGTTATAAATTAAAAAATGCCGCAGATAACACTGCGGCATTTTTGTCTCTTTAATTAATCAACCAATAACTCTC
TTTTTGCTTATTATGAGAAA

Product: hypothetical protein

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MTLKNRFMRSATWENMATEDGHMTDKLYDIYEELAKGEVGLIVTGYANIVEEEKPNAGMMGMYNDSFIEEYKKLTELVHL
NDSKIVMQLAYGGTKTTYNVGERVIFAPSDVCERGTQTQGKAMTKDEIDYIVDAFAKASLRAKESGFDGVEIHAAHTYLI
NQFLSPYYNRREDEYGGSLDNRMRFLLEIYAEIRKQVGDDYPILVKLTASEFFEGGLTFDETRIICKKLEEIGVDGIIIS
GNIHGNASTLVGESFDGYTLQEEGYFHEYGNVISQDVNVPVITVGGLNDINAIEKIAEETNIQYFALSRPLLAEPQLIKR
WKEGNRAEVDCERCSKCRTKRGNFCVVYKKRKPKKECA

Sequences:

>Translated_358_residues
MTLKNRFMRSATWENMATEDGHMTDKLYDIYEELAKGEVGLIVTGYANIVEEEKPNAGMMGMYNDSFIEEYKKLTELVHL
NDSKIVMQLAYGGTKTTYNVGERVIFAPSDVCERGTQTQGKAMTKDEIDYIVDAFAKASLRAKESGFDGVEIHAAHTYLI
NQFLSPYYNRREDEYGGSLDNRMRFLLEIYAEIRKQVGDDYPILVKLTASEFFEGGLTFDETRIICKKLEEIGVDGIIIS
GNIHGNASTLVGESFDGYTLQEEGYFHEYGNVISQDVNVPVITVGGLNDINAIEKIAEETNIQYFALSRPLLAEPQLIKR
WKEGNRAEVDCERCSKCRTKRGNFCVVYKKRKPKKECA
>Mature_357_residues
TLKNRFMRSATWENMATEDGHMTDKLYDIYEELAKGEVGLIVTGYANIVEEEKPNAGMMGMYNDSFIEEYKKLTELVHLN
DSKIVMQLAYGGTKTTYNVGERVIFAPSDVCERGTQTQGKAMTKDEIDYIVDAFAKASLRAKESGFDGVEIHAAHTYLIN
QFLSPYYNRREDEYGGSLDNRMRFLLEIYAEIRKQVGDDYPILVKLTASEFFEGGLTFDETRIICKKLEEIGVDGIIISG
NIHGNASTLVGESFDGYTLQEEGYFHEYGNVISQDVNVPVITVGGLNDINAIEKIAEETNIQYFALSRPLLAEPQLIKRW
KEGNRAEVDCERCSKCRTKRGNFCVVYKKRKPKKECA

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=341, Percent_Identity=29.3255131964809, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1787939, Length=352, Percent_Identity=26.7045454545455, Blast_Score=105, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17559802, Length=343, Percent_Identity=31.4868804664723, Blast_Score=150, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17565138, Length=337, Percent_Identity=31.4540059347181, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17540738, Length=346, Percent_Identity=30.9248554913295, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17559804, Length=340, Percent_Identity=31.1764705882353, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI72001454, Length=345, Percent_Identity=31.5942028985507, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17564188, Length=341, Percent_Identity=29.6187683284457, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17566914, Length=302, Percent_Identity=28.1456953642384, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17566916, Length=341, Percent_Identity=25.5131964809384, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321973, Length=179, Percent_Identity=30.1675977653631, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6325086, Length=198, Percent_Identity=30.3030303030303, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 40570; Mature: 40439

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKNRFMRSATWENMATEDGHMTDKLYDIYEELAKGEVGLIVTGYANIVEEEKPNAGMM
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECHHHHHHCCCCCCCEE
GMYNDSFIEEYKKLTELVHLNDSKIVMQLAYGGTKTTYNVGERVIFAPSDVCERGTQTQG
CCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEECCCEEEECCHHHHHCCCCCCC
KAMTKDEIDYIVDAFAKASLRAKESGFDGVEIHAAHTYLINQFLSPYYNRREDEYGGSLD
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHCCCCHH
NRMRFLLEIYAEIRKQVGDDYPILVKLTASEFFEGGLTFDETRIICKKLEEIGVDGIIIS
HHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEE
GNIHGNASTLVGESFDGYTLQEEGYFHEYGNVISQDVNVPVITVGGLNDINAIEKIAEET
CCCCCCCHHEECCCCCCEEECCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHC
NIQYFALSRPLLAEPQLIKRWKEGNRAEVDCERCSKCRTKRGNFCVVYKKRKPKKECA
CCEEEEECCCCCCCCHHHHHHHCCCCCEECHHHHHHHHCCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
TLKNRFMRSATWENMATEDGHMTDKLYDIYEELAKGEVGLIVTGYANIVEEEKPNAGMM
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECHHHHHHCCCCCCCEE
GMYNDSFIEEYKKLTELVHLNDSKIVMQLAYGGTKTTYNVGERVIFAPSDVCERGTQTQG
CCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEECCCEEEECCHHHHHCCCCCCC
KAMTKDEIDYIVDAFAKASLRAKESGFDGVEIHAAHTYLINQFLSPYYNRREDEYGGSLD
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHCCCCHH
NRMRFLLEIYAEIRKQVGDDYPILVKLTASEFFEGGLTFDETRIICKKLEEIGVDGIIIS
HHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEE
GNIHGNASTLVGESFDGYTLQEEGYFHEYGNVISQDVNVPVITVGGLNDINAIEKIAEET
CCCCCCCHHEECCCCCCEEECCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHC
NIQYFALSRPLLAEPQLIKRWKEGNRAEVDCERCSKCRTKRGNFCVVYKKRKPKKECA
CCEEEEECCCCCCCCHHHHHHHCCCCCEECHHHHHHHHCCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]