Click here to switch to the map view.

The map label for this gene is gloA [H]

Identifier: 59713302

GI number: 59713302

Start: 134323

End: 134694

Strand: Reverse

Name: gloA [H]

Synonym: VF_A0119

Alternate gene names: 59713302

Gene position: 134694-134323 (Counterclockwise)

Preceding gene: 59713305

Following gene: 59713301

Centisome position: 10.12

GC content: 40.32

Gene sequence:

>372_bases
ATGCAATTTCTACATACGATGATCAGAGTGACAGACTTAAAACAATCTATTGAATTTTATACCAAGGTTTTGGGGATGAA
AGTGCTCGATCACAGCGAAAACAGTGAATATCGCTACACACTCGTTTTCGTTGGTTATGAAGAAGGCGGAACAAGTATCG
AGTTGACGTATAACTGGGATACCGATAATTACGACATGGGTAATGCGTTTGGTCATTTAGCTTTAGGTGTCCAAGATATT
TATGCAGCTTGCGATAACATCAAAGCACTAGGTGGCAACGTGACTCGTGAAGCTGGGCCAGTAAAAGGCGGTACACGCGT
ATTGCGTTTATTACAGATCCTGATGGTTATCAAATTGAGCTTATTCAATTAA

Upstream 100 bases:

>100_bases
ATTATTAAATTAATAAAAAATCATAATTTCATAAATTTAGTCATCTTGCGTATCGTTAGATTATACAAACGACAACAATC
AGCACTGGCAAGGTGAAATC

Downstream 100 bases:

>100_bases
GCAAATAAGAGGATTTATTGATGAAAAATGCACTATTTCAACCAATGCAACTTGGTAATCTGACACTAAAAAACCGTATC
GTTATGCCTCCTATGACTCG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 123; Mature: 123

Protein sequence:

>123_residues
MQFLHTMIRVTDLKQSIEFYTKVLGMKVLDHSENSEYRYTLVFVGYEEGGTSIELTYNWDTDNYDMGNAFGHLALGVQDI
YAACDNIKALGGNVTREAGPVKGGTRVLRLLQILMVIKLSLFN

Sequences:

>Translated_123_residues
MQFLHTMIRVTDLKQSIEFYTKVLGMKVLDHSENSEYRYTLVFVGYEEGGTSIELTYNWDTDNYDMGNAFGHLALGVQDI
YAACDNIKALGGNVTREAGPVKGGTRVLRLLQILMVIKLSLFN
>Mature_123_residues
MQFLHTMIRVTDLKQSIEFYTKVLGMKVLDHSENSEYRYTLVFVGYEEGGTSIELTYNWDTDNYDMGNAFGHLALGVQDI
YAACDNIKALGGNVTREAGPVKGGTRVLRLLQILMVIKLSLFN

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=134, Percent_Identity=30.5970149253731, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1787940, Length=114, Percent_Identity=59.6491228070175, Blast_Score=142, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6323639, Length=109, Percent_Identity=37.6146788990826, Blast_Score=70, Evalue=1e-13,
Organism=Drosophila melanogaster, GI20129743, Length=147, Percent_Identity=31.9727891156463, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 13858; Mature: 13858

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFLHTMIRVTDLKQSIEFYTKVLGMKVLDHSENSEYRYTLVFVGYEEGGTSIELTYNWD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCEEEEEEECC
TDNYDMGNAFGHLALGVQDIYAACDNIKALGGNVTREAGPVKGGTRVLRLLQILMVIKLS
CCCCCCCCCHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LFN
CCC
>Mature Secondary Structure
MQFLHTMIRVTDLKQSIEFYTKVLGMKVLDHSENSEYRYTLVFVGYEEGGTSIELTYNWD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCEEEEEEECC
TDNYDMGNAFGHLALGVQDIYAACDNIKALGGNVTREAGPVKGGTRVLRLLQILMVIKLS
CCCCCCCCCHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LFN
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 9719565 [H]