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The map label for this gene is 59713273

Identifier: 59713273

GI number: 59713273

Start: 98085

End: 99218

Strand: Reverse

Name: 59713273

Synonym: VF_A0090

Alternate gene names: NA

Gene position: 99218-98085 (Counterclockwise)

Preceding gene: 59713274

Following gene: 59713270

Centisome position: 7.46

GC content: 29.89

Gene sequence:

>1134_bases
ATGAAAAATATTATTATCTATTTACTCCCAACTTATATCTTTTCAGTTTCATTAAATGCTAATGATGATATGTGTGATAC
TGATTCGTATGAAGAAAACATGAGAATGCAAGTATATTCACACATTGTTTCTGATTTTAGGTACAAATGGATAAATAATA
CAATTCCTTACCAATTTGATAATACTCCACCAGTTCGAAAAAGAGAGGCCGTTTTAAGTGCTATTCAACATATAGAAAGT
GTTACCAACCTAAAATTTATAGAAAGAAATAACATAAATAAAGATAAATACCCTGATTTTATAAATATTAATAGTGAAGA
TGGATCCGGATGCTCTGCTAAGATAGGAAGACGTGGTGGAGAGCAGTTCATGAAAATAGGTGGCTGTACTCGCCTCTCTG
TTGTACATGAATTTGCACATGCTATTGGAATGTTTCATGAACATAACCGTTCAGACAGAGACTCATATATTACCATTCAC
TGGGATCGAATTCGGAAAGATAAAAGACAAAATTTTCAAATAAATGGCTCCGAATCAGAAAATATTAGGGGTTATGATTA
CGCTTCAATAACCCATTATTCTACACACACTTTTTCAACAAGTGCGTGGCCAACAATCACTACTCTCAATGGTGAAAAAA
TAGGTAGAAGAGATGGCTTAAGTATGGGAGATATATATACTATAAATTATATGTATCCAAGCTATACTGATCATAAAGAA
GTTGTGCTTTATAGTGACAAAGGCCATAAAGGAAAAAAATATAAATTAAAAAATAGCTCTCCTTTATTAGGTGATTTTTC
AAAAGAACTATCATCATTAATCATTCCTGATGGCTTTTCAGTTATTTTATATAAAAGTGCAAATTATACAGGTGAATATA
CTTCATTATCATATATGGATGATAATGCACAAATTATCGATTCATTTAATAACAATGTATCTTCAGTTCACGTAATTAAA
AGTCAAACTCAAAGAGGCGTTATAGGCGATGTCTATATTTATGAAAATCAATATAATAATGATACTGAATTGTTCAAATT
AATTTCATTGGATCAAAAAAATGAGTATGGACACTTTCCAATTAACAAATCAAATAACCAATATTGGAAGTATTTAAGAA
CATTAAAAAACTAA

Upstream 100 bases:

>100_bases
TTAATAAAAACAATTTAGTGCAAGTGTATATATTTACAGTTTTTTAATTTTAAAACTAATATTAAAATAAGAAATCACTT
TAATTTGAGTATGTTTTATA

Downstream 100 bases:

>100_bases
ACTATTTAATCAAAATATAATTGATTAATTAAATAACCTTGTTTTTGTTCATCAACACCCGATTTATCCACCAATACTTC
TTTCTGCTTTAATATATTAA

Product: astacin-like metalloendopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MKNIIIYLLPTYIFSVSLNANDDMCDTDSYEENMRMQVYSHIVSDFRYKWINNTIPYQFDNTPPVRKREAVLSAIQHIES
VTNLKFIERNNINKDKYPDFININSEDGSGCSAKIGRRGGEQFMKIGGCTRLSVVHEFAHAIGMFHEHNRSDRDSYITIH
WDRIRKDKRQNFQINGSESENIRGYDYASITHYSTHTFSTSAWPTITTLNGEKIGRRDGLSMGDIYTINYMYPSYTDHKE
VVLYSDKGHKGKKYKLKNSSPLLGDFSKELSSLIIPDGFSVILYKSANYTGEYTSLSYMDDNAQIIDSFNNNVSSVHVIK
SQTQRGVIGDVYIYENQYNNDTELFKLISLDQKNEYGHFPINKSNNQYWKYLRTLKN

Sequences:

>Translated_377_residues
MKNIIIYLLPTYIFSVSLNANDDMCDTDSYEENMRMQVYSHIVSDFRYKWINNTIPYQFDNTPPVRKREAVLSAIQHIES
VTNLKFIERNNINKDKYPDFININSEDGSGCSAKIGRRGGEQFMKIGGCTRLSVVHEFAHAIGMFHEHNRSDRDSYITIH
WDRIRKDKRQNFQINGSESENIRGYDYASITHYSTHTFSTSAWPTITTLNGEKIGRRDGLSMGDIYTINYMYPSYTDHKE
VVLYSDKGHKGKKYKLKNSSPLLGDFSKELSSLIIPDGFSVILYKSANYTGEYTSLSYMDDNAQIIDSFNNNVSSVHVIK
SQTQRGVIGDVYIYENQYNNDTELFKLISLDQKNEYGHFPINKSNNQYWKYLRTLKN
>Mature_377_residues
MKNIIIYLLPTYIFSVSLNANDDMCDTDSYEENMRMQVYSHIVSDFRYKWINNTIPYQFDNTPPVRKREAVLSAIQHIES
VTNLKFIERNNINKDKYPDFININSEDGSGCSAKIGRRGGEQFMKIGGCTRLSVVHEFAHAIGMFHEHNRSDRDSYITIH
WDRIRKDKRQNFQINGSESENIRGYDYASITHYSTHTFSTSAWPTITTLNGEKIGRRDGLSMGDIYTINYMYPSYTDHKE
VVLYSDKGHKGKKYKLKNSSPLLGDFSKELSSLIIPDGFSVILYKSANYTGEYTSLSYMDDNAQIIDSFNNNVSSVHVIK
SQTQRGVIGDVYIYENQYNNDTELFKLISLDQKNEYGHFPINKSNNQYWKYLRTLKN

Specific function: Zinc metallendopeptidase that cleaves preferentially on amino-terminal side of aspartate-containing substrates [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ricin B-type lectin domain [H]

Homologues:

Organism=Homo sapiens, GI153070264, Length=217, Percent_Identity=34.1013824884793, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI157502169, Length=171, Percent_Identity=34.5029239766082, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI5453579, Length=194, Percent_Identity=34.5360824742268, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI4502421, Length=194, Percent_Identity=34.5360824742268, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI6912724, Length=196, Percent_Identity=33.6734693877551, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI22547221, Length=214, Percent_Identity=33.1775700934579, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI153070262, Length=221, Percent_Identity=28.9592760180996, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI71995692, Length=191, Percent_Identity=33.5078534031414, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71989620, Length=196, Percent_Identity=33.1632653061224, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71981168, Length=191, Percent_Identity=32.9842931937173, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71983640, Length=221, Percent_Identity=34.841628959276, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71983649, Length=221, Percent_Identity=34.841628959276, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71989946, Length=203, Percent_Identity=33.4975369458128, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71989942, Length=203, Percent_Identity=33.4975369458128, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI115532764, Length=190, Percent_Identity=34.2105263157895, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71986189, Length=189, Percent_Identity=31.2169312169312, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25149641, Length=191, Percent_Identity=32.9842931937173, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71981193, Length=267, Percent_Identity=28.4644194756554, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25152806, Length=193, Percent_Identity=33.6787564766839, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25150905, Length=195, Percent_Identity=33.8461538461538, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI72000269, Length=197, Percent_Identity=30.9644670050761, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71980967, Length=244, Percent_Identity=29.5081967213115, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17534131, Length=163, Percent_Identity=33.1288343558282, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71983097, Length=153, Percent_Identity=32.0261437908497, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71996833, Length=200, Percent_Identity=32, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17568533, Length=186, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17509235, Length=165, Percent_Identity=29.6969696969697, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71996019, Length=246, Percent_Identity=27.6422764227642, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17562122, Length=153, Percent_Identity=30.718954248366, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71986142, Length=191, Percent_Identity=28.7958115183246, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71993705, Length=213, Percent_Identity=27.6995305164319, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71991562, Length=308, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI193210526, Length=166, Percent_Identity=34.3373493975904, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17562124, Length=139, Percent_Identity=32.3741007194245, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17568805, Length=196, Percent_Identity=30.6122448979592, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI212645453, Length=192, Percent_Identity=29.1666666666667, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI86562912, Length=246, Percent_Identity=24.7967479674797, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115532766, Length=98, Percent_Identity=39.7959183673469, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24644493, Length=196, Percent_Identity=33.1632653061224, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21355491, Length=191, Percent_Identity=32.9842931937173, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24649626, Length=194, Percent_Identity=32.4742268041237, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17136740, Length=218, Percent_Identity=33.0275229357798, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24649765, Length=218, Percent_Identity=33.0275229357798, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI20129545, Length=201, Percent_Identity=37.3134328358209, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19921382, Length=194, Percent_Identity=31.9587628865979, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45549230, Length=194, Percent_Identity=32.9896907216495, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19921388, Length=246, Percent_Identity=30.8943089430894, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19921386, Length=197, Percent_Identity=30.9644670050761, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI19921384, Length=199, Percent_Identity=31.6582914572864, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24643089, Length=193, Percent_Identity=31.0880829015544, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19921390, Length=197, Percent_Identity=29.4416243654822, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24646966, Length=250, Percent_Identity=27.2, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001506
- InterPro:   IPR006026
- InterPro:   IPR008997
- InterPro:   IPR000772 [H]

Pfam domain/function: PF01400 Astacin; PF00652 Ricin_B_lectin [H]

EC number: =3.4.24.76 [H]

Molecular weight: Translated: 43731; Mature: 43731

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIIIYLLPTYIFSVSLNANDDMCDTDSYEENMRMQVYSHIVSDFRYKWINNTIPYQFD
CCCEEEEEEECEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
NTPPVRKREAVLSAIQHIESVTNLKFIERNNINKDKYPDFININSEDGSGCSAKIGRRGG
CCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCEEEECCCCCCCCHHHHHCCCC
EQFMKIGGCTRLSVVHEFAHAIGMFHEHNRSDRDSYITIHWDRIRKDKRQNFQINGSESE
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHCCEEECCCCCC
NIRGYDYASITHYSTHTFSTSAWPTITTLNGEKIGRRDGLSMGDIYTINYMYPSYTDHKE
CCCCCEEEEEEEEECCEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCE
VVLYSDKGHKGKKYKLKNSSPLLGDFSKELSSLIIPDGFSVILYKSANYTGEYTSLSYMD
EEEECCCCCCCCEEEECCCCCCCCHHHHHHHHEECCCCCEEEEEECCCCCCCCEEEEEEC
DNAQIIDSFNNNVSSVHVIKSQTQRGVIGDVYIYENQYNNDTELFKLISLDQKNEYGHFP
CCHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCHHHHEEEEECCCCCCCCCCC
INKSNNQYWKYLRTLKN
CCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKNIIIYLLPTYIFSVSLNANDDMCDTDSYEENMRMQVYSHIVSDFRYKWINNTIPYQFD
CCCEEEEEEECEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
NTPPVRKREAVLSAIQHIESVTNLKFIERNNINKDKYPDFININSEDGSGCSAKIGRRGG
CCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCEEEECCCCCCCCHHHHHCCCC
EQFMKIGGCTRLSVVHEFAHAIGMFHEHNRSDRDSYITIHWDRIRKDKRQNFQINGSESE
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHCCEEECCCCCC
NIRGYDYASITHYSTHTFSTSAWPTITTLNGEKIGRRDGLSMGDIYTINYMYPSYTDHKE
CCCCCEEEEEEEEECCEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCE
VVLYSDKGHKGKKYKLKNSSPLLGDFSKELSSLIIPDGFSVILYKSANYTGEYTSLSYMD
EEEECCCCCCCCEEEECCCCCCCCHHHHHHHHEECCCCCEEEEEECCCCCCCCEEEEEEC
DNAQIIDSFNNNVSSVHVIKSQTQRGVIGDVYIYENQYNNDTELFKLISLDQKNEYGHFP
CCHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCHHHHEEEEECCCCCCCCCCC
INKSNNQYWKYLRTLKN
CCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7771796; 7768917 [H]