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The map label for this gene is acrA [H]

Identifier: 172087825

GI number: 172087825

Start: 1294499

End: 1295617

Strand: Reverse

Name: acrA [H]

Synonym: VF_A1152

Alternate gene names: 172087825

Gene position: 1295617-1294499 (Counterclockwise)

Preceding gene: 59714336

Following gene: 59714332

Centisome position: 97.39

GC content: 42.63

Gene sequence:

>1119_bases
ATGAAATCCAAAATGCTTCTTGCTTCAATCATTAGTACAGCTTTACTCGTTGGCTGTAGTGATGAATCAACTGCTCAACG
AGCTCCGCAAGCCCCACTTGTTGGAACTTACACCGTTGAACCTATTTCGTACCAACAAGGAAAAACCTACATTGGTCGAA
TCGAAGCCATGGAAGATGCTTCAATCTCAGCTCAAGTTTCAGGATATATTCAATCTCGCCAGTTTAAAGAAGGTCAAATG
GTTGAGAAGGGTGATGTGCTTTTCCAAATTGATCCCTCTTCTTTCGAAGCCCAAGTTGCCAACGCAAAAGCCGGAATAAC
TCAGGCTAATGCAGCGCTTAAAAAAGCCCAGTTAGATTTTAATCGTGGCAAAAATCTATTACCTAAAGGCAATATTAGCC
AAGCTGAATTTGATGGTCTAACCGCGCAATTATTGAGTGCGGAAGCGCAAGTCGAGGCCGCTCAAGCGCAATTGAAGTTG
GCACAAGTTAACCTATCTTACACTACCATTACAGCGCCATTCACAGGTCGAATTAGTGACAGTAAAGCCAGCATTGGTGA
TTTAGTATCTCCTGCATCTGGAGTGTTAACCACTTTAGTTAGCTTAGACCCAATTCATACTCGTTTTACCATCAGTGAAC
GTGAGCGTTTGATGATGGGGATTGATGCGATTGAAGGTGATGGAAAAGGGGCGTCAAGCCAAGTGGAAGTGGTATTGAAC
CTTGAAAATGGTAAAGAGTATAAACATCTTGGCAAAGTCGATTTCATTGGTAACCGTATCGACAAAACAACCGGCACATT
AACACTGCGTGCTTTGGTTGATAACCCTGAACATACGCTACTTCCTGGTCAACATATCAATGTAAATCTTAGAGAGAAGC
AGCCAACAGAAGTCTTAGTGGTTCCAAGACGTGCAGTACAAAGTGATTTAGAAGGCGATTTTGTCATGGTATTAACCGAA
GGTAATATTGCAGAGCGTCGTAATGTCACTCTAGGCAAGCAATTACCTGAAGGCGTGATCATCAGTTCGGGAATTAAGGC
CAATGAACAAGTGTTAGTAAAAGGATTGCAACGTGTAAGAAACGGAGTTCAGGTTCGGTTAGAGAACACGGAGTCTTAA

Upstream 100 bases:

>100_bases
TTACAGACACATTCAGTAACATACTCGTTTTATTTAGATTAATAATTTTAACCATTTAACTGTCAGAATATAGGTAATTC
AACAGTTAAATGGTTATATT

Downstream 100 bases:

>100_bases
TATGCTAAGTCGATTTTTTATACAGCGTCCTAAATTTGCTCTGGTTATTTCTATTATCCTCACTCTTGCCGGAGCCATTG
CATTAGCAGTATTACCTGTT

Product: multidrug efflux system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKSKMLLASIISTALLVGCSDESTAQRAPQAPLVGTYTVEPISYQQGKTYIGRIEAMEDASISAQVSGYIQSRQFKEGQM
VEKGDVLFQIDPSSFEAQVANAKAGITQANAALKKAQLDFNRGKNLLPKGNISQAEFDGLTAQLLSAEAQVEAAQAQLKL
AQVNLSYTTITAPFTGRISDSKASIGDLVSPASGVLTTLVSLDPIHTRFTISERERLMMGIDAIEGDGKGASSQVEVVLN
LENGKEYKHLGKVDFIGNRIDKTTGTLTLRALVDNPEHTLLPGQHINVNLREKQPTEVLVVPRRAVQSDLEGDFVMVLTE
GNIAERRNVTLGKQLPEGVIISSGIKANEQVLVKGLQRVRNGVQVRLENTES

Sequences:

>Translated_372_residues
MKSKMLLASIISTALLVGCSDESTAQRAPQAPLVGTYTVEPISYQQGKTYIGRIEAMEDASISAQVSGYIQSRQFKEGQM
VEKGDVLFQIDPSSFEAQVANAKAGITQANAALKKAQLDFNRGKNLLPKGNISQAEFDGLTAQLLSAEAQVEAAQAQLKL
AQVNLSYTTITAPFTGRISDSKASIGDLVSPASGVLTTLVSLDPIHTRFTISERERLMMGIDAIEGDGKGASSQVEVVLN
LENGKEYKHLGKVDFIGNRIDKTTGTLTLRALVDNPEHTLLPGQHINVNLREKQPTEVLVVPRRAVQSDLEGDFVMVLTE
GNIAERRNVTLGKQLPEGVIISSGIKANEQVLVKGLQRVRNGVQVRLENTES
>Mature_372_residues
MKSKMLLASIISTALLVGCSDESTAQRAPQAPLVGTYTVEPISYQQGKTYIGRIEAMEDASISAQVSGYIQSRQFKEGQM
VEKGDVLFQIDPSSFEAQVANAKAGITQANAALKKAQLDFNRGKNLLPKGNISQAEFDGLTAQLLSAEAQVEAAQAQLKL
AQVNLSYTTITAPFTGRISDSKASIGDLVSPASGVLTTLVSLDPIHTRFTISERERLMMGIDAIEGDGKGASSQVEVVLN
LENGKEYKHLGKVDFIGNRIDKTTGTLTLRALVDNPEHTLLPGQHINVNLREKQPTEVLVVPRRAVQSDLEGDFVMVLTE
GNIAERRNVTLGKQLPEGVIISSGIKANEQVLVKGLQRVRNGVQVRLENTES

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=367, Percent_Identity=34.3324250681199, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI1786668, Length=355, Percent_Identity=35.7746478873239, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1789665, Length=368, Percent_Identity=34.5108695652174, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI87082036, Length=347, Percent_Identity=29.971181556196, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI87081951, Length=143, Percent_Identity=38.4615384615385, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081796, Length=196, Percent_Identity=30.6122448979592, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790519, Length=262, Percent_Identity=27.0992366412214, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40138; Mature: 40138

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKMLLASIISTALLVGCSDESTAQRAPQAPLVGTYTVEPISYQQGKTYIGRIEAMEDA
CCCHHHHHHHHHHHHEEECCCCHHHHHCCCCCEEEEEEECCCCCCCCCEEEEEEEECCCC
SISAQVSGYIQSRQFKEGQMVEKGDVLFQIDPSSFEAQVANAKAGITQANAALKKAQLDF
CCCHHHHHHHHHCCCCCCCEEECCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHCCC
NRGKNLLPKGNISQAEFDGLTAQLLSAEAQVEAAQAQLKLAQVNLSYTTITAPFTGRISD
CCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEEECCEECCCCC
SKASIGDLVSPASGVLTTLVSLDPIHTRFTISERERLMMGIDAIEGDGKGASSQVEVVLN
CCCCHHHHCCCHHHHHHHHHHCCCCEEEEEECHHHEEEEECEEECCCCCCCCCEEEEEEE
LENGKEYKHLGKVDFIGNRIDKTTGTLTLRALVDNPEHTLLPGQHINVNLREKQPTEVLV
CCCCCCHHHCCCEEECCCCCCCCCCEEEEEEEECCCCCEECCCCEEEEEECCCCCCEEEE
VPRRAVQSDLEGDFVMVLTEGNIAERRNVTLGKQLPEGVIISSGIKANEQVLVKGLQRVR
ECCHHHHCCCCCCEEEEEECCCCCCCCCCEECCCCCCCEEEECCCCCCHHHHHHHHHHHC
NGVQVRLENTES
CCEEEEEECCCC
>Mature Secondary Structure
MKSKMLLASIISTALLVGCSDESTAQRAPQAPLVGTYTVEPISYQQGKTYIGRIEAMEDA
CCCHHHHHHHHHHHHEEECCCCHHHHHCCCCCEEEEEEECCCCCCCCCEEEEEEEECCCC
SISAQVSGYIQSRQFKEGQMVEKGDVLFQIDPSSFEAQVANAKAGITQANAALKKAQLDF
CCCHHHHHHHHHCCCCCCCEEECCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHCCC
NRGKNLLPKGNISQAEFDGLTAQLLSAEAQVEAAQAQLKLAQVNLSYTTITAPFTGRISD
CCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEEECCEECCCCC
SKASIGDLVSPASGVLTTLVSLDPIHTRFTISERERLMMGIDAIEGDGKGASSQVEVVLN
CCCCHHHHCCCHHHHHHHHHHCCCCEEEEEECHHHEEEEECEEECCCCCCCCCEEEEEEE
LENGKEYKHLGKVDFIGNRIDKTTGTLTLRALVDNPEHTLLPGQHINVNLREKQPTEVLV
CCCCCCHHHCCCEEECCCCCCCCCCEEEEEEEECCCCCEECCCCEEEEEECCCCCCEEEE
VPRRAVQSDLEGDFVMVLTEGNIAERRNVTLGKQLPEGVIISSGIKANEQVLVKGLQRVR
ECCHHHHCCCCCCEEEEEECCCCCCCCCCEECCCCCCCEEEECCCCCCHHHHHHHHHHHC
NGVQVRLENTES
CCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]