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The map label for this gene is bioB [H]

Identifier: 172087814

GI number: 172087814

Start: 844068

End: 845120

Strand: Reverse

Name: bioB [H]

Synonym: VF_A1189

Alternate gene names: 172087814

Gene position: 845120-844068 (Counterclockwise)

Preceding gene: 59713932

Following gene: 172087813

Centisome position: 63.53

GC content: 41.79

Gene sequence:

>1053_bases
GTGGAAGTGAGACATAACTGGACCGTAGCTGAAGTACAAACCTTGATGGATAAACCGTTTATGGATTTGATCTTTGAGGC
TCAATTAGTACATAGAAAATATCAACAAGCTAATCATGTACAGGTGAGCACACTACTTTCAATTAAAACAGGAGCCTGTC
CTGAAGACTGTAAGTATTGCCCGCAGAGTGCTCACTACCGCACTGATGTTGATCGTGAACGTTTAATGGAAGTGGAGAGT
GTTTTAGACGCTGCGAAAAAAGCAAAAAATTCAGGTTCTACTCGTTTTTGTATGGGGGCTGCTTGGAAGAACCCAAAAGA
ACGTGATATGCCATATCTATTGGACATGATTAAAGGCGTGAAAGAAATGGGCCTTGAGACATGTATGACGCTAGGTATGA
TCACCGCCGATCAAGCGGGTGAATTATCCGAAGCAGGCTTAGATTACTATAACCATAACCTCGATACTTCTCCTGAATTT
TACGGCAATATTATTACCACTCGAACTTATCAAGATCGTTTAGATACGCTATCTCACGTTCGTGATGCTGGGATGAAAAT
TTGTTCTGGTGGCATCATTGGTATGGGGGAAAGTGCAAGTGATCGTGCTGGTCTATTTGTTGAATTAGCGAATCTACCTC
AACATCCAGAATCTGTTCCTGTAAATATGCTGGTAAAAGTAAAAGGAACCCCATTAGAAGACGCTGAAGACGTTGACCCA
TTTGATTTCATTCGTTTGATCGCCGTTGCTCGCATCATGATGCCTGAATCTGCTGTACGTCTTTCAGCAGGACGTGAAAG
CATGAATGAGCAAATGCAAGCGTTATGCTTTATGGCTGGTGCCAACTCAGTCTTTTATGGATGTAAGTTGCTCACTACGC
CAAATCCAGATGAAGACACAGACATGCAACTTTTCAAAAAATTGGGTGTGAATAGTGAACAAGTAGCGCAAAAACCTGAT
CAAATTCAAGAGCATGAATTGCTTGATAGAGTGGCAGAACGTGTTGCAGCTCGTCCAAATAAAGATGACTTGTTCTATGA
AGCAAGCGTTTAA

Upstream 100 bases:

>100_bases
TTCTTTAGGTTGACAGTGTATCGTTTAACCGTAGACTAGCCAAGTATAAAAACAGTCATTACATGGTTGTTGAGCCATAA
AATAATAAAGGAATTTACAC

Downstream 100 bases:

>100_bases
TCAACGTATTAGCTCTGCGCTTAATCAACGTAAGCAAGCAGGGCTAAATCGCTCACGTCGAGTGGTATCGCAAGGAAATC
AGGCCACATTAGTTGTTGAT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MEVRHNWTVAEVQTLMDKPFMDLIFEAQLVHRKYQQANHVQVSTLLSIKTGACPEDCKYCPQSAHYRTDVDRERLMEVES
VLDAAKKAKNSGSTRFCMGAAWKNPKERDMPYLLDMIKGVKEMGLETCMTLGMITADQAGELSEAGLDYYNHNLDTSPEF
YGNIITTRTYQDRLDTLSHVRDAGMKICSGGIIGMGESASDRAGLFVELANLPQHPESVPVNMLVKVKGTPLEDAEDVDP
FDFIRLIAVARIMMPESAVRLSAGRESMNEQMQALCFMAGANSVFYGCKLLTTPNPDEDTDMQLFKKLGVNSEQVAQKPD
QIQEHELLDRVAERVAARPNKDDLFYEASV

Sequences:

>Translated_350_residues
MEVRHNWTVAEVQTLMDKPFMDLIFEAQLVHRKYQQANHVQVSTLLSIKTGACPEDCKYCPQSAHYRTDVDRERLMEVES
VLDAAKKAKNSGSTRFCMGAAWKNPKERDMPYLLDMIKGVKEMGLETCMTLGMITADQAGELSEAGLDYYNHNLDTSPEF
YGNIITTRTYQDRLDTLSHVRDAGMKICSGGIIGMGESASDRAGLFVELANLPQHPESVPVNMLVKVKGTPLEDAEDVDP
FDFIRLIAVARIMMPESAVRLSAGRESMNEQMQALCFMAGANSVFYGCKLLTTPNPDEDTDMQLFKKLGVNSEQVAQKPD
QIQEHELLDRVAERVAARPNKDDLFYEASV
>Mature_350_residues
MEVRHNWTVAEVQTLMDKPFMDLIFEAQLVHRKYQQANHVQVSTLLSIKTGACPEDCKYCPQSAHYRTDVDRERLMEVES
VLDAAKKAKNSGSTRFCMGAAWKNPKERDMPYLLDMIKGVKEMGLETCMTLGMITADQAGELSEAGLDYYNHNLDTSPEF
YGNIITTRTYQDRLDTLSHVRDAGMKICSGGIIGMGESASDRAGLFVELANLPQHPESVPVNMLVKVKGTPLEDAEDVDP
FDFIRLIAVARIMMPESAVRLSAGRESMNEQMQALCFMAGANSVFYGCKLLTTPNPDEDTDMQLFKKLGVNSEQVAQKPD
QIQEHELLDRVAERVAARPNKDDLFYEASV

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=350, Percent_Identity=67.1428571428571, Blast_Score=505, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6321725, Length=318, Percent_Identity=47.4842767295598, Blast_Score=316, Evalue=3e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 39188; Mature: 39188

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVRHNWTVAEVQTLMDKPFMDLIFEAQLVHRKYQQANHVQVSTLLSIKTGACPEDCKYC
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEHHHHEEECCCCCCHHHHHC
PQSAHYRTDVDRERLMEVESVLDAAKKAKNSGSTRFCMGAAWKNPKERDMPYLLDMIKGV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHH
KEMGLETCMTLGMITADQAGELSEAGLDYYNHNLDTSPEFYGNIITTRTYQDRLDTLSHV
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHCCEEEECCHHHHHHHHHHH
RDAGMKICSGGIIGMGESASDRAGLFVELANLPQHPESVPVNMLVKVKGTPLEDAEDVDP
HHHHHHHHCCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCH
FDFIRLIAVARIMMPESAVRLSAGRESMNEQMQALCFMAGANSVFYGCKLLTTPNPDEDT
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECEEEECCCCCCCCH
DMQLFKKLGVNSEQVAQKPDQIQEHELLDRVAERVAARPNKDDLFYEASV
HHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCC
>Mature Secondary Structure
MEVRHNWTVAEVQTLMDKPFMDLIFEAQLVHRKYQQANHVQVSTLLSIKTGACPEDCKYC
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEHHHHEEECCCCCCHHHHHC
PQSAHYRTDVDRERLMEVESVLDAAKKAKNSGSTRFCMGAAWKNPKERDMPYLLDMIKGV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHH
KEMGLETCMTLGMITADQAGELSEAGLDYYNHNLDTSPEFYGNIITTRTYQDRLDTLSHV
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHCCEEEECCHHHHHHHHHHH
RDAGMKICSGGIIGMGESASDRAGLFVELANLPQHPESVPVNMLVKVKGTPLEDAEDVDP
HHHHHHHHCCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCH
FDFIRLIAVARIMMPESAVRLSAGRESMNEQMQALCFMAGANSVFYGCKLLTTPNPDEDT
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECEEEECCCCCCCCH
DMQLFKKLGVNSEQVAQKPDQIQEHELLDRVAERVAARPNKDDLFYEASV
HHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA