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The map label for this gene is pck [H]

Identifier: 59713085

GI number: 59713085

Start: 2775187

End: 2776809

Strand: Direct

Name: pck [H]

Synonym: VF_2478

Alternate gene names: 59713085

Gene position: 2775187-2776809 (Clockwise)

Preceding gene: 59713084

Following gene: 59713086

Centisome position: 95.78

GC content: 42.14

Gene sequence:

>1623_bases
ATGACTGTTATGGAACACAAGAAGGCTGCACTACTCGATCTAACTCAATATGGTCTTACTGGGGTTACAGACGTACTACG
TAATCCAAGCTATGAGCAACTATTTGAAGAAGAGACACGTCCAGATCTTGAAGGTTATGAACGTGGTGTAATGACAGAGC
TTGGCTCAGTTGCGGTAGATACTGGTATATTTACTGGTCGTTCACCAAAAGATAAATACATCGTAAAAGATGACACTACC
AAAGATACTCTATGGTGGTCAGATCAAGGAAAGAACGACAACAAAGCCATCACGCCAGCAGTTTGGGATGATCTAAAGTC
GCTAGTTACAACACAACTATCGGGTAAACGCTTATTTGTTATTGATGGTTTCTGTGGCGCCAATCCAGATACTCGTTTAA
ACGTTCGCATCATTACAGAAGTAGCATGGCAAGCACACTTTGTGAAAAACATGTTCATTCGTCCAACAGAAGCAGAACTT
GAAAACTTCGAGCCTGATTTCGTAGTAATGAATGGTGCAAAAGTCACTAACCCTAATTATGAAAAGCACGGATTAAACTC
TGAAAACTTCGTGGCTTTCAACTTAACAGAACGCGTTCAAATCATCGGTGGTACTTGGTACGGCGGTGAAATGAAAAAAG
GTATGTTCGCAATGATGAACTACCTACTTCCACTTAAAGGTATTGCTTCAATGCACTGTTCGGCTAACGTCGGTGAAAAA
GGTGATGTTGCGGTATTCTTTGGTTTATCAGGAACAGGAAAAACCACGCTTTCAACCGATCCTAAACGCCAACTAATTGG
TGATGATGAACACGGTTGGGATGACGATGGCATCTTCAACTTTGAAGGCGGTTGCTACGCTAAAACCATCCGCCTTTCAA
AAGAAGCTGAGCCTGATATCTATAATGCAATCCGCCGTGATGCATTATTAGAAAACGTCACAGTACGTAGCGACAGCTCT
ATTGATTTTAATGATGGCTCTAAAACAGAAAACACTCGTGTTTCTTACCCTATCTATCATATCGATAACATCGTTAAACC
AGTTTCAAAAGCTGGCCACGCTAAAAAAGTCATCTTCTTAACAGCGGATGCGTTTGGTGTTCTACCGCCGGTAGCGAAAC
TCACACCAGAACAAACTAAGTATCACTTCCTATCAGGTTTTACCGCAAAACTTGCAGGTACTGAACGTGGTATTACAGAA
CCAACACCAACCTTCTCTGCTGCATTTGGTGCTGCGTTCTTAACGCTTCATCCAACACAATACGCAGAAGTACTGGTTAA
ACGTATGGAAGCGGCAGGCGCTGAAGCTTACATCGTAAACACGGGTTGGAATGGCACAGGAAAACGTATTTCAATTCAAG
ATACACGTGGCATTATTGATGCGATTTTAGATGGTTCAATTGACCAAGCTAAAACCAAAAATATCCCTGTATTTAACTTA
GAAGTACCGACATCACTACCTGGTGTTGATGCCTCTATTCTTGACCCTCGTGACACGTATACTGACCCACTACAATGGGA
CAGCAAAGCGGAAGATTTAGCGCAACGCTTTATCAAAAACTTCGCTCAATACACAGATAACGAGGAAGGAAAAGCGTTAG
TTAAAGCGGGTCCTCAGCTATAA

Upstream 100 bases:

>100_bases
TCATCTCCCTGTTTTCAATTTACCGCGTTGTTAGCATGATTAGCAGAATACAAATAATTAGAAATATTAAAATCCCTACA
AAATCCTCTAAGGAGCACCT

Downstream 100 bases:

>100_bases
CCTACGCCTGATTACTCATAAAAACCAAGCCCTTCTCTCCTTATGAGAAGGGCTTTTTTATACCCTTAGCTCAAGAACGC
AATAAAGACAGGAGATCATA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MTVMEHKKAALLDLTQYGLTGVTDVLRNPSYEQLFEEETRPDLEGYERGVMTELGSVAVDTGIFTGRSPKDKYIVKDDTT
KDTLWWSDQGKNDNKAITPAVWDDLKSLVTTQLSGKRLFVIDGFCGANPDTRLNVRIITEVAWQAHFVKNMFIRPTEAEL
ENFEPDFVVMNGAKVTNPNYEKHGLNSENFVAFNLTERVQIIGGTWYGGEMKKGMFAMMNYLLPLKGIASMHCSANVGEK
GDVAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGIFNFEGGCYAKTIRLSKEAEPDIYNAIRRDALLENVTVRSDSS
IDFNDGSKTENTRVSYPIYHIDNIVKPVSKAGHAKKVIFLTADAFGVLPPVAKLTPEQTKYHFLSGFTAKLAGTERGITE
PTPTFSAAFGAAFLTLHPTQYAEVLVKRMEAAGAEAYIVNTGWNGTGKRISIQDTRGIIDAILDGSIDQAKTKNIPVFNL
EVPTSLPGVDASILDPRDTYTDPLQWDSKAEDLAQRFIKNFAQYTDNEEGKALVKAGPQL

Sequences:

>Translated_540_residues
MTVMEHKKAALLDLTQYGLTGVTDVLRNPSYEQLFEEETRPDLEGYERGVMTELGSVAVDTGIFTGRSPKDKYIVKDDTT
KDTLWWSDQGKNDNKAITPAVWDDLKSLVTTQLSGKRLFVIDGFCGANPDTRLNVRIITEVAWQAHFVKNMFIRPTEAEL
ENFEPDFVVMNGAKVTNPNYEKHGLNSENFVAFNLTERVQIIGGTWYGGEMKKGMFAMMNYLLPLKGIASMHCSANVGEK
GDVAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGIFNFEGGCYAKTIRLSKEAEPDIYNAIRRDALLENVTVRSDSS
IDFNDGSKTENTRVSYPIYHIDNIVKPVSKAGHAKKVIFLTADAFGVLPPVAKLTPEQTKYHFLSGFTAKLAGTERGITE
PTPTFSAAFGAAFLTLHPTQYAEVLVKRMEAAGAEAYIVNTGWNGTGKRISIQDTRGIIDAILDGSIDQAKTKNIPVFNL
EVPTSLPGVDASILDPRDTYTDPLQWDSKAEDLAQRFIKNFAQYTDNEEGKALVKAGPQL
>Mature_539_residues
TVMEHKKAALLDLTQYGLTGVTDVLRNPSYEQLFEEETRPDLEGYERGVMTELGSVAVDTGIFTGRSPKDKYIVKDDTTK
DTLWWSDQGKNDNKAITPAVWDDLKSLVTTQLSGKRLFVIDGFCGANPDTRLNVRIITEVAWQAHFVKNMFIRPTEAELE
NFEPDFVVMNGAKVTNPNYEKHGLNSENFVAFNLTERVQIIGGTWYGGEMKKGMFAMMNYLLPLKGIASMHCSANVGEKG
DVAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGIFNFEGGCYAKTIRLSKEAEPDIYNAIRRDALLENVTVRSDSSI
DFNDGSKTENTRVSYPIYHIDNIVKPVSKAGHAKKVIFLTADAFGVLPPVAKLTPEQTKYHFLSGFTAKLAGTERGITEP
TPTFSAAFGAAFLTLHPTQYAEVLVKRMEAAGAEAYIVNTGWNGTGKRISIQDTRGIIDAILDGSIDQAKTKNIPVFNLE
VPTSLPGVDASILDPRDTYTDPLQWDSKAEDLAQRFIKNFAQYTDNEEGKALVKAGPQL

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=530, Percent_Identity=79.4339622641509, Blast_Score=898, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322950, Length=530, Percent_Identity=44.3396226415094, Blast_Score=447, Evalue=1e-126,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 59660; Mature: 59529

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVMEHKKAALLDLTQYGLTGVTDVLRNPSYEQLFEEETRPDLEGYERGVMTELGSVAVD
CCCCCCCHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHEE
TGIFTGRSPKDKYIVKDDTTKDTLWWSDQGKNDNKAITPAVWDDLKSLVTTQLSGKRLFV
CCEECCCCCCCCEEEECCCCCCEEEECCCCCCCCCEECHHHHHHHHHHHHHHCCCCEEEE
IDGFCGANPDTRLNVRIITEVAWQAHFVKNMFIRPTEAELENFEPDFVVMNGAKVTNPNY
EECCCCCCCCCEEEEEEEEHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCEEECCCCH
EKHGLNSENFVAFNLTERVQIIGGTWYGGEMKKGMFAMMNYLLPLKGIASMHCSANVGEK
HHCCCCCCCEEEEEECCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GDVAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGIFNFEGGCYAKTIRLSKEAEPDI
CCEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCCHH
YNAIRRDALLENVTVRSDSSIDFNDGSKTENTRVSYPIYHIDNIVKPVSKAGHAKKVIFL
HHHHHHHHHHHCEEECCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEE
TADAFGVLPPVAKLTPEQTKYHFLSGFTAKLAGTERGITEPTPTFSAAFGAAFLTLHPTQ
ECCCCCCCCCHHHCCCCHHHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHEEEEEECCHH
YAEVLVKRMEAAGAEAYIVNTGWNGTGKRISIQDTRGIIDAILDGSIDQAKTKNIPVFNL
HHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCHHHCCCCCEEEE
EVPTSLPGVDASILDPRDTYTDPLQWDSKAEDLAQRFIKNFAQYTDNEEGKALVKAGPQL
ECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCC
>Mature Secondary Structure 
TVMEHKKAALLDLTQYGLTGVTDVLRNPSYEQLFEEETRPDLEGYERGVMTELGSVAVD
CCCCCCHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHEE
TGIFTGRSPKDKYIVKDDTTKDTLWWSDQGKNDNKAITPAVWDDLKSLVTTQLSGKRLFV
CCEECCCCCCCCEEEECCCCCCEEEECCCCCCCCCEECHHHHHHHHHHHHHHCCCCEEEE
IDGFCGANPDTRLNVRIITEVAWQAHFVKNMFIRPTEAELENFEPDFVVMNGAKVTNPNY
EECCCCCCCCCEEEEEEEEHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCEEECCCCH
EKHGLNSENFVAFNLTERVQIIGGTWYGGEMKKGMFAMMNYLLPLKGIASMHCSANVGEK
HHCCCCCCCEEEEEECCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GDVAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGIFNFEGGCYAKTIRLSKEAEPDI
CCEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCCHH
YNAIRRDALLENVTVRSDSSIDFNDGSKTENTRVSYPIYHIDNIVKPVSKAGHAKKVIFL
HHHHHHHHHHHCEEECCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEE
TADAFGVLPPVAKLTPEQTKYHFLSGFTAKLAGTERGITEPTPTFSAAFGAAFLTLHPTQ
ECCCCCCCCCHHHCCCCHHHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHEEEEEECCHH
YAEVLVKRMEAAGAEAYIVNTGWNGTGKRISIQDTRGIIDAILDGSIDQAKTKNIPVFNL
HHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCHHHCCCCCEEEE
EVPTSLPGVDASILDPRDTYTDPLQWDSKAEDLAQRFIKNFAQYTDNEEGKALVKAGPQL
ECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA