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The map label for this gene is rsmD [H]

Identifier: 59713061

GI number: 59713061

Start: 2753833

End: 2754435

Strand: Direct

Name: rsmD [H]

Synonym: VF_2454

Alternate gene names: 59713061

Gene position: 2753833-2754435 (Clockwise)

Preceding gene: 59713053

Following gene: 59713062

Centisome position: 95.04

GC content: 39.47

Gene sequence:

>603_bases
ATGGTTAAACGTAAACAACACTCACCTCGCACTTCTGGCACAAATGGTCGCTCCGGAAGCATTCGTATTATCAGTGGACG
CTGGCGCGGACGCAAACTTCCAGTACATGATGCAGAAGGATTACGACCAACCACGGACCGTGTAAAAGAGACTGTTTTTA
ACTGGTTAGCACCTGATATTCCTCGAGCTCGTTGTTTGGATCTTTTTTCTGGCAGCGGCGGTTTAGGTTTTGAAGCCGCT
TCTCGTGGTGCTGAACACGTCGTAATGCTTGAATTAAATAAACAAGCTCATGCACAACTTAAACAAAATGCAGAGAGTTT
AAAAGCAGATAATATCGAAATTCATCATACCGATGCATTAACGTTTTTAAATCAAGCTGCAACTCCATTTGATGTTGTTT
TTATTGACCCTCCATTCAGAAAAGATTTATTAGAAGAAACCTTAACAAAATTAGAAAATAATGGATGGTTAGCAGCAAAC
GCTATGATATACATTGAAGCAGAAAAAGAATTAAACTCATTACCGGTTCCTGCACATTGGCATCTAAGCAAAGATAAAAC
AGCAGGACAAGTTACCTTCAAATTATATGAAAGAGAGGAATAG

Upstream 100 bases:

>100_bases
CAATAATCCGCGTTTCTTTTTGCCTGTCATTGGGAAATCCTAGAATTAAATTGGTACAATTTTCTCGTATACTATCACTT
TCTGAGCGGTCAAAAAATCT

Downstream 100 bases:

>100_bases
ATGAAAGGATTGATCATTCTTGCTAAAGCTGCCATTGCCTTTGTGTGGTTTATTTTAATTTTAAATTTCTTTATGCCATT
CCCAGGTAAAGCCGCCATTG

Product: methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MVKRKQHSPRTSGTNGRSGSIRIISGRWRGRKLPVHDAEGLRPTTDRVKETVFNWLAPDIPRARCLDLFSGSGGLGFEAA
SRGAEHVVMLELNKQAHAQLKQNAESLKADNIEIHHTDALTFLNQAATPFDVVFIDPPFRKDLLEETLTKLENNGWLAAN
AMIYIEAEKELNSLPVPAHWHLSKDKTAGQVTFKLYEREE

Sequences:

>Translated_200_residues
MVKRKQHSPRTSGTNGRSGSIRIISGRWRGRKLPVHDAEGLRPTTDRVKETVFNWLAPDIPRARCLDLFSGSGGLGFEAA
SRGAEHVVMLELNKQAHAQLKQNAESLKADNIEIHHTDALTFLNQAATPFDVVFIDPPFRKDLLEETLTKLENNGWLAAN
AMIYIEAEKELNSLPVPAHWHLSKDKTAGQVTFKLYEREE
>Mature_200_residues
MVKRKQHSPRTSGTNGRSGSIRIISGRWRGRKLPVHDAEGLRPTTDRVKETVFNWLAPDIPRARCLDLFSGSGGLGFEAA
SRGAEHVVMLELNKQAHAQLKQNAESLKADNIEIHHTDALTFLNQAATPFDVVFIDPPFRKDLLEETLTKLENNGWLAAN
AMIYIEAEKELNSLPVPAHWHLSKDKTAGQVTFKLYEREE

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=183, Percent_Identity=57.3770491803279, Blast_Score=223, Evalue=6e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22461; Mature: 22461

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKRKQHSPRTSGTNGRSGSIRIISGRWRGRKLPVHDAEGLRPTTDRVKETVFNWLAPDI
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
PRARCLDLFSGSGGLGFEAASRGAEHVVMLELNKQAHAQLKQNAESLKADNIEIHHTDAL
CHHHHHHHHCCCCCCCHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEEEHHHH
TFLNQAATPFDVVFIDPPFRKDLLEETLTKLENNGWLAANAMIYIEAEKELNSLPVPAHW
HHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCCCCEE
HLSKDKTAGQVTFKLYEREE
ECCCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MVKRKQHSPRTSGTNGRSGSIRIISGRWRGRKLPVHDAEGLRPTTDRVKETVFNWLAPDI
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
PRARCLDLFSGSGGLGFEAASRGAEHVVMLELNKQAHAQLKQNAESLKADNIEIHHTDAL
CHHHHHHHHCCCCCCCHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEEEHHHH
TFLNQAATPFDVVFIDPPFRKDLLEETLTKLENNGWLAANAMIYIEAEKELNSLPVPAHW
HHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCCCCEE
HLSKDKTAGQVTFKLYEREE
ECCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]