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The map label for this gene is hslU [H]

Identifier: 59712885

GI number: 59712885

Start: 2560355

End: 2561689

Strand: Direct

Name: hslU [H]

Synonym: VF_2278

Alternate gene names: 59712885

Gene position: 2560355-2561689 (Clockwise)

Preceding gene: 59712884

Following gene: 59712887

Centisome position: 88.36

GC content: 41.87

Gene sequence:

>1335_bases
ATGTCTGAGATGACTCCTCGTGAAATTGTACACGAATTAGATAGCCATATTATTGGTCAAGATAAAGCAAAACGTTCAGT
TGCTATTGCTCTTCGTAACCGCTGGCGCCGTATGCAACTGGCTCCTGAGTTGCGCACAGAAGTAACACCAAAAAACATTC
TGATGATTGGCCCAACAGGTGTGGGTAAAACTGAAATCGCTCGCCGTTTAGCAAAACTTGCAAACGCACCTTTCATCAAA
GTTGAAGCAACTAAATTCACTGAAGTGGGCTACGTTGGTAAAGAAGTAGAAAGCATCATCCGTGATTTGACCGATGTTGC
TATCAAAATGACGCATCAACAAGCCGTTGAAAAAGTAAAATTCCGTGCTGAAGAGCACGCAGAAGATCGCATTCTAGATA
TCTTACTGCCACCTGCTCGCGATGCATGGGGCAATAATGAAGAAGGCAATAACGATTCTGGCACTCGTCAATCTTTCCGT
AAAAAACTACGTGAAGGTAAATTAGACGATAAAGAAATCGAAGTTGATGTTGCTGCACCTCAAGTTGGCGTGGAAATCAT
GGCTCCTCCTGGTATGGAAGAGATGACAAACCAGCTTCAAGGTATGTTCCAAAACCTTTCTGGTGGCAATACAACCAAAA
AACGCAAAATGAAAATCGTTGATGCACTTAAAGCACTAACAGAAGAAGAAGGTGCGAAGCTGGTTAATCCTGAGGAGCTA
AAAGAACAAGCGATCTTCAATGTAGAAAACCACGGTATCGTGTTTATCGATGAAATTGATAAAATCTGTAAAGGTTCAAA
CTCTCACTCTGGTGATGTATCTCGTGAAGGTGTTCAACGTGATTTACTTCCATTAGTAGAAGGCAGCACGGTTAGCACTA
AACATGGCATGGTGAAAACCGACCATATGCTATTCATCACGTCAGGTGCATTCCAAATGGCAAAACCATCGGACTTGATC
CCTGAATTACAAGGCCGCTTACCAATTCGTGTAGAACTTGAAGCACTAACTGCGGATGATTTCAAACGCATTTTAACTGA
GCCTAATGCATCATTAACGGAACAATACATTGCTCTATTAGCGACCGAAAATGTAAAAGTTGAATTTACGGAAGATGGCA
TCAGTCGTATTGCTGAATCTGCATTCCAAGTAAATGAAACCACTGAAAACATCGGTGCTCGTCGTCTACACACAGTGATG
GAACGACTAATGGAAGAGATTTCTTATGATGCTTCTGAAAAAAATGGCGAAAGCTTAATTGTTGATGCTGAGTATGTAAG
TTCACGCCTAGGTGAGCTTGTTGCTGATGAAGACCTAAGCCGCTTCATTCTGTAA

Upstream 100 bases:

>100_bases
GCAACTAAAGCACTGACTATTGCTGGCGATATCGATGTATACACCAACCACAACCACACAGTTGAAGAACTGAACGCTTA
ACCAAGAATAAGGAATTACC

Downstream 100 bases:

>100_bases
TCTCTCATAACTTAAAAATAAAGCCTCGCAAGCATTAACTTGCGAGGCTTTTTTCTAATCTTTGTTGCCTACAGTTTAAG
CTGAAACAATCTCCCATGAG

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSEMTPREIVHELDSHIIGQDKAKRSVAIALRNRWRRMQLAPELRTEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIK
VEATKFTEVGYVGKEVESIIRDLTDVAIKMTHQQAVEKVKFRAEEHAEDRILDILLPPARDAWGNNEEGNNDSGTRQSFR
KKLREGKLDDKEIEVDVAAPQVGVEIMAPPGMEEMTNQLQGMFQNLSGGNTTKKRKMKIVDALKALTEEEGAKLVNPEEL
KEQAIFNVENHGIVFIDEIDKICKGSNSHSGDVSREGVQRDLLPLVEGSTVSTKHGMVKTDHMLFITSGAFQMAKPSDLI
PELQGRLPIRVELEALTADDFKRILTEPNASLTEQYIALLATENVKVEFTEDGISRIAESAFQVNETTENIGARRLHTVM
ERLMEEISYDASEKNGESLIVDAEYVSSRLGELVADEDLSRFIL

Sequences:

>Translated_444_residues
MSEMTPREIVHELDSHIIGQDKAKRSVAIALRNRWRRMQLAPELRTEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIK
VEATKFTEVGYVGKEVESIIRDLTDVAIKMTHQQAVEKVKFRAEEHAEDRILDILLPPARDAWGNNEEGNNDSGTRQSFR
KKLREGKLDDKEIEVDVAAPQVGVEIMAPPGMEEMTNQLQGMFQNLSGGNTTKKRKMKIVDALKALTEEEGAKLVNPEEL
KEQAIFNVENHGIVFIDEIDKICKGSNSHSGDVSREGVQRDLLPLVEGSTVSTKHGMVKTDHMLFITSGAFQMAKPSDLI
PELQGRLPIRVELEALTADDFKRILTEPNASLTEQYIALLATENVKVEFTEDGISRIAESAFQVNETTENIGARRLHTVM
ERLMEEISYDASEKNGESLIVDAEYVSSRLGELVADEDLSRFIL
>Mature_443_residues
SEMTPREIVHELDSHIIGQDKAKRSVAIALRNRWRRMQLAPELRTEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKV
EATKFTEVGYVGKEVESIIRDLTDVAIKMTHQQAVEKVKFRAEEHAEDRILDILLPPARDAWGNNEEGNNDSGTRQSFRK
KLREGKLDDKEIEVDVAAPQVGVEIMAPPGMEEMTNQLQGMFQNLSGGNTTKKRKMKIVDALKALTEEEGAKLVNPEELK
EQAIFNVENHGIVFIDEIDKICKGSNSHSGDVSREGVQRDLLPLVEGSTVSTKHGMVKTDHMLFITSGAFQMAKPSDLIP
ELQGRLPIRVELEALTADDFKRILTEPNASLTEQYIALLATENVKVEFTEDGISRIAESAFQVNETTENIGARRLHTVME
RLMEEISYDASEKNGESLIVDAEYVSSRLGELVADEDLSRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790366, Length=444, Percent_Identity=77.9279279279279, Blast_Score=704, Evalue=0.0,
Organism=Escherichia coli, GI1786642, Length=100, Percent_Identity=46, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319704, Length=488, Percent_Identity=25, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648289, Length=256, Percent_Identity=28.515625, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648291, Length=256, Percent_Identity=28.515625, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 49597; Mature: 49466

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEMTPREIVHELDSHIIGQDKAKRSVAIALRNRWRRMQLAPELRTEVTPKNILMIGPTG
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEECCCC
VGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVESIIRDLTDVAIKMTHQQAVEKVK
CCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRAEEHAEDRILDILLPPARDAWGNNEEGNNDSGTRQSFRKKLREGKLDDKEIEVDVAAP
HHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECC
QVGVEIMAPPGMEEMTNQLQGMFQNLSGGNTTKKRKMKIVDALKALTEEEGAKLVNPEEL
CCCEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
KEQAIFNVENHGIVFIDEIDKICKGSNSHSGDVSREGVQRDLLPLVEGSTVSTKHGMVKT
HHHHHEEECCCCEEEEHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEE
DHMLFITSGAFQMAKPSDLIPELQGRLPIRVELEALTADDFKRILTEPNASLTEQYIALL
CCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHCCCCHHHHHHHHHHH
ATENVKVEFTEDGISRIAESAFQVNETTENIGARRLHTVMERLMEEISYDASEKNGESLI
EECCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
VDAEYVSSRLGELVADEDLSRFIL
EEHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEMTPREIVHELDSHIIGQDKAKRSVAIALRNRWRRMQLAPELRTEVTPKNILMIGPTG
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEECCCC
VGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVESIIRDLTDVAIKMTHQQAVEKVK
CCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRAEEHAEDRILDILLPPARDAWGNNEEGNNDSGTRQSFRKKLREGKLDDKEIEVDVAAP
HHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECC
QVGVEIMAPPGMEEMTNQLQGMFQNLSGGNTTKKRKMKIVDALKALTEEEGAKLVNPEEL
CCCEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
KEQAIFNVENHGIVFIDEIDKICKGSNSHSGDVSREGVQRDLLPLVEGSTVSTKHGMVKT
HHHHHEEECCCCEEEEHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEE
DHMLFITSGAFQMAKPSDLIPELQGRLPIRVELEALTADDFKRILTEPNASLTEQYIALL
CCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHCCCCHHHHHHHHHHH
ATENVKVEFTEDGISRIAESAFQVNETTENIGARRLHTVMERLMEEISYDASEKNGESLI
EECCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
VDAEYVSSRLGELVADEDLSRFIL
EEHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA