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The map label for this gene is mraW [H]

Identifier: 59712816

GI number: 59712816

Start: 2482208

End: 2483158

Strand: Reverse

Name: mraW [H]

Synonym: VF_2209

Alternate gene names: 59712816

Gene position: 2483158-2482208 (Counterclockwise)

Preceding gene: 59712817

Following gene: 59712815

Centisome position: 85.7

GC content: 39.85

Gene sequence:

>951_bases
ATGTCTGAACAATTCCAACACGTATCCGTTCTTCTTCACGAATCTATTGATGGTTTAGCTATCAAACCTGATGGTATTTA
CATCGATGGTACGTTTGGTCGTGGTGGCCACAGCCGTCAGATCTTATCTCAATTAGGTGAGAATGGTCGCTTGTATAGTA
TTGACCGTGATCCTCAAGCAATTGCTGAAGCAAAAACGATTACTGATCCTAAATTTACTATCATTCATGGTCCATTTTCA
GGCCTTAAACAATATGTTGAAGAGCTTGATCTTGTAGGAAAAATTGATGGAGTATTACTGGATTTAGGTGTGTCATCTCC
ACAACTAGATGATGCTGAACGTGGTTTTAGCTTTATGAAAGATGGCCCATTGGATATGCGTATGGATCCAACATCAGGCA
TTCCTGTATCTCAGTGGTTACAAGAAGCGGATGTTGAAGATATTACTTGGGTAATTCGTGAGTTTGGTGAAGATAAGCAC
GCTTGGCGTATTGCTAAAGGTATCGTTGCTTATCTCGAAAATGAAGAAAATGAACCTTTAACTCGAACTAGTCAGTTAGC
TAAGTTGATTTCTGAGGTTGCTCCCAAGAGCTTTAAAGAGAAAAAGCACCCAGCAACGCGTGCATTCCAAGCATTCCGAA
TTTATATCAATAGTGAGTTAGACGAAATTGATACAGCACTAAAAGGGGCATTAGATGTATTGGCACCTGAAGGTCGTTTA
TCAGTGATTAGCTTCCACTCACTAGAAGATCGTATGGTTAAGCATTTTATTCGTAAAGAAAGTAAAGGACCGCAAGTACC
TCATGGTTTGCCGTTAACGGAAGAACAAATTAAAGCATTAGGCAGTGCTAAAATGAAGCCTGTTGGTAAAGCCATCAAAC
CAACTAAGAATGAAGTGAATGAGAATGTGCGTTCACGTAGTTCTGTTCTACGTATTGCTGAGCGTTTATGA

Upstream 100 bases:

>100_bases
AGAATTATCACTAATTTGACACACATTCCTGCATTTTTAGGTTAAGCTTCGGCTTAATTTTAACTGCTTATTCATATAAT
TACGGTAGTAAATACTCACT

Downstream 100 bases:

>100_bases
GTAAAGATACCGCTTCACAACCAAGTCTTACAAAACTTATCGGCCTAGACATTTTTGGTGTAGGCCGCCTTCATGCAATT
CTACTAATTTGTATCTTTTT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSEQFQHVSVLLHESIDGLAIKPDGIYIDGTFGRGGHSRQILSQLGENGRLYSIDRDPQAIAEAKTITDPKFTIIHGPFS
GLKQYVEELDLVGKIDGVLLDLGVSSPQLDDAERGFSFMKDGPLDMRMDPTSGIPVSQWLQEADVEDITWVIREFGEDKH
AWRIAKGIVAYLENEENEPLTRTSQLAKLISEVAPKSFKEKKHPATRAFQAFRIYINSELDEIDTALKGALDVLAPEGRL
SVISFHSLEDRMVKHFIRKESKGPQVPHGLPLTEEQIKALGSAKMKPVGKAIKPTKNEVNENVRSRSSVLRIAERL

Sequences:

>Translated_316_residues
MSEQFQHVSVLLHESIDGLAIKPDGIYIDGTFGRGGHSRQILSQLGENGRLYSIDRDPQAIAEAKTITDPKFTIIHGPFS
GLKQYVEELDLVGKIDGVLLDLGVSSPQLDDAERGFSFMKDGPLDMRMDPTSGIPVSQWLQEADVEDITWVIREFGEDKH
AWRIAKGIVAYLENEENEPLTRTSQLAKLISEVAPKSFKEKKHPATRAFQAFRIYINSELDEIDTALKGALDVLAPEGRL
SVISFHSLEDRMVKHFIRKESKGPQVPHGLPLTEEQIKALGSAKMKPVGKAIKPTKNEVNENVRSRSSVLRIAERL
>Mature_315_residues
SEQFQHVSVLLHESIDGLAIKPDGIYIDGTFGRGGHSRQILSQLGENGRLYSIDRDPQAIAEAKTITDPKFTIIHGPFSG
LKQYVEELDLVGKIDGVLLDLGVSSPQLDDAERGFSFMKDGPLDMRMDPTSGIPVSQWLQEADVEDITWVIREFGEDKHA
WRIAKGIVAYLENEENEPLTRTSQLAKLISEVAPKSFKEKKHPATRAFQAFRIYINSELDEIDTALKGALDVLAPEGRLS
VISFHSLEDRMVKHFIRKESKGPQVPHGLPLTEEQIKALGSAKMKPVGKAIKPTKNEVNENVRSRSSVLRIAERL

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=344, Percent_Identity=37.5, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI165377202, Length=203, Percent_Identity=39.9014778325123, Blast_Score=125, Evalue=4e-29,
Organism=Escherichia coli, GI1786270, Length=316, Percent_Identity=65.1898734177215, Blast_Score=424, Evalue=1e-120,
Organism=Drosophila melanogaster, GI62472493, Length=332, Percent_Identity=35.8433734939759, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI28571637, Length=332, Percent_Identity=35.8433734939759, Blast_Score=176, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 35231; Mature: 35099

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQFQHVSVLLHESIDGLAIKPDGIYIDGTFGRGGHSRQILSQLGENGRLYSIDRDPQA
CCCHHHHHHHHHHHCCCCEEECCCCEEEECCCCCCCCHHHHHHHCCCCCCEEEECCCHHH
IAEAKTITDPKFTIIHGPFSGLKQYVEELDLVGKIDGVLLDLGVSSPQLDDAERGFSFMK
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHCCHHHC
DGPLDMRMDPTSGIPVSQWLQEADVEDITWVIREFGEDKHAWRIAKGIVAYLENEENEPL
CCCCCEEECCCCCCCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCH
TRTSQLAKLISEVAPKSFKEKKHPATRAFQAFRIYINSELDEIDTALKGALDVLAPEGRL
HHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCE
SVISFHSLEDRMVKHFIRKESKGPQVPHGLPLTEEQIKALGSAKMKPVGKAIKPTKNEVN
EEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHCCCCHHHHH
ENVRSRSSVLRIAERL
HHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SEQFQHVSVLLHESIDGLAIKPDGIYIDGTFGRGGHSRQILSQLGENGRLYSIDRDPQA
CCHHHHHHHHHHHCCCCEEECCCCEEEECCCCCCCCHHHHHHHCCCCCCEEEECCCHHH
IAEAKTITDPKFTIIHGPFSGLKQYVEELDLVGKIDGVLLDLGVSSPQLDDAERGFSFMK
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHCCHHHC
DGPLDMRMDPTSGIPVSQWLQEADVEDITWVIREFGEDKHAWRIAKGIVAYLENEENEPL
CCCCCEEECCCCCCCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCH
TRTSQLAKLISEVAPKSFKEKKHPATRAFQAFRIYINSELDEIDTALKGALDVLAPEGRL
HHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCE
SVISFHSLEDRMVKHFIRKESKGPQVPHGLPLTEEQIKALGSAKMKPVGKAIKPTKNEVN
EEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHCCCCHHHHH
ENVRSRSSVLRIAERL
HHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA