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The map label for this gene is ftsI [H]

Identifier: 59712814

GI number: 59712814

Start: 2480149

End: 2481888

Strand: Reverse

Name: ftsI [H]

Synonym: VF_2207

Alternate gene names: 59712814

Gene position: 2481888-2480149 (Counterclockwise)

Preceding gene: 59712815

Following gene: 59712813

Centisome position: 85.66

GC content: 43.16

Gene sequence:

>1740_bases
ATGAAATTACCAAATAGAAAAAAAAACAATAAACGCATAACCAAAGCGCCACCAACATTTATCCAGTGGCGCTTTGGCGT
TATTGTTTTCTTTGTCTTTCTTGCCTTAGCTATTCTTATTGCGCGAGCCGCTTATATCCAAGTTATTGAACCAGATAACC
TTGTTAAGCAAGGTGATATGCGTTCTATCCGTGTTAAATCTCTCCCTTCTGCCAGAGGCATTATCTCTGATCGTAATGAT
GAACAGCTTGCCGTAAGTGTACCTGTTGATGCGGTTTGGGCTGATCCTGTGACGATCTATAAAGAAGGTGGTTTGGCTGA
GATTGAACGCTGGTATGCGCTTGCGGATGTATTAGGGCTTAAGCGTAAAGAGTTAATTTCTAAAATTGAAAAAAACAAAA
AACGTCGTTTTATTTATCTTCAACGCCAAATATCCCCGGCGGTTGCTGCCTATATTAAAAAACTGAAATTAGCGGGTATT
GGTTTAAAAGCTGAATCACGTCGTTATTACCCAACTGGTGAAATCAGTGCCCATGTTATTGGTGTAACAGGTATTGATAG
TCATGGCCTTGAAGGGGTAGAACGTAGTTATGATGGTTGGTTAACTGGTGAGCCGGGACGTAGAACTGTACGTAAAGACC
GTTATGGCCGAGTGGTTGAAAATATTTCTCTAAAAGAGAAACAAGAAGGGAAGCCATTAACTCTAAGTATCGATCAGCGT
TTACAGGCGATTGCTTACCGTGCAATTAAGCAAGCTGTATCTGACTTTAGAGCGATGTCTGGCTCAGTTGTGATGATCGA
TATTAAGACGGGCGAAGTACTTGCAATGGTAAATGCCCCTTCTTATAACCCGAACAATCGTGAGCAGTTACAAAGCTTTA
GAATGCGTAACCGTGTGATTACCGATACGTTAGAACCGGGCTCTACAGTAAAACCTTTTGTTGTATTAGCTGCTTTAGAT
GGTGGAACTGCGAACTTAGATACGATTATTGATACTGGTAATGGCATCATGCGTATTGGCGGTAGTCGTGTGCGTGATAG
CTCGAAAGTTGGTAAAGCCGATCTAACTACTATCTTGAAGAAATCAAGTAACATCGGTGTTGCGCACTTAGCGTTAGGAA
TGCCTTTGCAAGAGTTGTTAGCGGAATACGCGGCTGTTGGTCTTGCTGACCCATCAGGCTTAAACTTAATTGGTGAGGCT
ACGGGTATTTTCCCTGATCGTCGTCGTTGGTCTGATTTCGAGATCGCCACATTAGCCTTTGGATATGGTTTGTCGGTTAC
GCCAATCCAATTAGCGCACGCTTATGCCACATTAGGGGCTTATGGTGAATATAGACCACTTTCAATTTTGAAAACAACTG
GACCAACACCGGGTAAGCAAGTGGTTAATCGCCAACATGCAAAAGCGGTATTAGAAATGATGGAAACCGTAACTCAACCT
GGTGGTACAGCAACAAGAGCGGCAGTACCTGGCTACCGAGTTGCCGCTAAAACTGGTACTTCACGAAAAGCGGTTCGTGG
TGGTTACAGTGATGAATACATGACGGTGACAGCAGGTGTTGCACCAGTCAGTAACCCCCGAATTGCGTTAGTTACTATCG
TGAACCAACCACAAGGTGATGAGTATTACGCAGGTCAAGTTGCGGCACCTATCTTTGGTCAGGTCATGAAAGATGCGTTG
CAAATTTTAAACGTAGCGCCAGATGAAAGTACCGTAAAACATATCGATGTATTGAATTAG

Upstream 100 bases:

>100_bases
AAAGCGCTCTTGCTGAGCATAGCCGAGTTCAAGCTCGCTCAGTTCGAGAGTTAGATATGGAACGCCCTGCTCCAGATAAA
GAAGTAATTATAAAACTACG

Downstream 100 bases:

>100_bases
GAAAGTGATGATGCAGTTATCTGAATTACTGTTGCCTTGGTGCGATTTAAATCAATCTGTGGCGATTTCATCTATGACAT
TGGACAGTCGCCAAGTTTCT

Product: transpeptidase (penicillin-binding protein 3)

Products: NA

Alternate protein names: Penicillin-binding protein 3; PBP-3; Peptidoglycan glycosyltransferase 3 [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MKLPNRKKNNKRITKAPPTFIQWRFGVIVFFVFLALAILIARAAYIQVIEPDNLVKQGDMRSIRVKSLPSARGIISDRND
EQLAVSVPVDAVWADPVTIYKEGGLAEIERWYALADVLGLKRKELISKIEKNKKRRFIYLQRQISPAVAAYIKKLKLAGI
GLKAESRRYYPTGEISAHVIGVTGIDSHGLEGVERSYDGWLTGEPGRRTVRKDRYGRVVENISLKEKQEGKPLTLSIDQR
LQAIAYRAIKQAVSDFRAMSGSVVMIDIKTGEVLAMVNAPSYNPNNREQLQSFRMRNRVITDTLEPGSTVKPFVVLAALD
GGTANLDTIIDTGNGIMRIGGSRVRDSSKVGKADLTTILKKSSNIGVAHLALGMPLQELLAEYAAVGLADPSGLNLIGEA
TGIFPDRRRWSDFEIATLAFGYGLSVTPIQLAHAYATLGAYGEYRPLSILKTTGPTPGKQVVNRQHAKAVLEMMETVTQP
GGTATRAAVPGYRVAAKTGTSRKAVRGGYSDEYMTVTAGVAPVSNPRIALVTIVNQPQGDEYYAGQVAAPIFGQVMKDAL
QILNVAPDESTVKHIDVLN

Sequences:

>Translated_579_residues
MKLPNRKKNNKRITKAPPTFIQWRFGVIVFFVFLALAILIARAAYIQVIEPDNLVKQGDMRSIRVKSLPSARGIISDRND
EQLAVSVPVDAVWADPVTIYKEGGLAEIERWYALADVLGLKRKELISKIEKNKKRRFIYLQRQISPAVAAYIKKLKLAGI
GLKAESRRYYPTGEISAHVIGVTGIDSHGLEGVERSYDGWLTGEPGRRTVRKDRYGRVVENISLKEKQEGKPLTLSIDQR
LQAIAYRAIKQAVSDFRAMSGSVVMIDIKTGEVLAMVNAPSYNPNNREQLQSFRMRNRVITDTLEPGSTVKPFVVLAALD
GGTANLDTIIDTGNGIMRIGGSRVRDSSKVGKADLTTILKKSSNIGVAHLALGMPLQELLAEYAAVGLADPSGLNLIGEA
TGIFPDRRRWSDFEIATLAFGYGLSVTPIQLAHAYATLGAYGEYRPLSILKTTGPTPGKQVVNRQHAKAVLEMMETVTQP
GGTATRAAVPGYRVAAKTGTSRKAVRGGYSDEYMTVTAGVAPVSNPRIALVTIVNQPQGDEYYAGQVAAPIFGQVMKDAL
QILNVAPDESTVKHIDVLN
>Mature_579_residues
MKLPNRKKNNKRITKAPPTFIQWRFGVIVFFVFLALAILIARAAYIQVIEPDNLVKQGDMRSIRVKSLPSARGIISDRND
EQLAVSVPVDAVWADPVTIYKEGGLAEIERWYALADVLGLKRKELISKIEKNKKRRFIYLQRQISPAVAAYIKKLKLAGI
GLKAESRRYYPTGEISAHVIGVTGIDSHGLEGVERSYDGWLTGEPGRRTVRKDRYGRVVENISLKEKQEGKPLTLSIDQR
LQAIAYRAIKQAVSDFRAMSGSVVMIDIKTGEVLAMVNAPSYNPNNREQLQSFRMRNRVITDTLEPGSTVKPFVVLAALD
GGTANLDTIIDTGNGIMRIGGSRVRDSSKVGKADLTTILKKSSNIGVAHLALGMPLQELLAEYAAVGLADPSGLNLIGEA
TGIFPDRRRWSDFEIATLAFGYGLSVTPIQLAHAYATLGAYGEYRPLSILKTTGPTPGKQVVNRQHAKAVLEMMETVTQP
GGTATRAAVPGYRVAAKTGTSRKAVRGGYSDEYMTVTAGVAPVSNPRIALVTIVNQPQGDEYYAGQVAAPIFGQVMKDAL
QILNVAPDESTVKHIDVLN

Specific function: Cell wall formation. Essential for the formation of a septum of the murein sacculus. Synthesis of cross-linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein; Periplasmic side. Note=The bulk of the molecule, except for the N-terminal membrane anchor region, protrudes into the periplasmic space, where it acts on murein (By similarity) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=554, Percent_Identity=51.8050541516246, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI1786854, Length=612, Percent_Identity=25.6535947712418, Blast_Score=125, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: =2.4.1.129 [H]

Molecular weight: Translated: 63210; Mature: 63210

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPNRKKNNKRITKAPPTFIQWRFGVIVFFVFLALAILIARAAYIQVIEPDNLVKQGDM
CCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHCCCCC
RSIRVKSLPSARGIISDRNDEQLAVSVPVDAVWADPVTIYKEGGLAEIERWYALADVLGL
CEEEEECCCCCCCCCCCCCCCEEEEECCCCEEECCCEEEEECCCHHHHHHHHHHHHHHCC
KRKELISKIEKNKKRRFIYLQRQISPAVAAYIKKLKLAGIGLKAESRRYYPTGEISAHVI
HHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEE
GVTGIDSHGLEGVERSYDGWLTGEPGRRTVRKDRYGRVVENISLKEKQEGKPLTLSIDQR
EEECCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCCEEEEHHHH
LQAIAYRAIKQAVSDFRAMSGSVVMIDIKTGEVLAMVNAPSYNPNNREQLQSFRMRNRVI
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHCCCE
TDTLEPGSTVKPFVVLAALDGGTANLDTIIDTGNGIMRIGGSRVRDSSKVGKADLTTILK
EECCCCCCCCCCEEEEEEECCCCCCCCEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHH
KSSNIGVAHLALGMPLQELLAEYAAVGLADPSGLNLIGEATGIFPDRRRWSDFEIATLAF
CCCCCCEEEHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEE
GYGLSVTPIQLAHAYATLGAYGEYRPLSILKTTGPTPGKQVVNRQHAKAVLEMMETVTQP
CCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
GGTATRAAVPGYRVAAKTGTSRKAVRGGYSDEYMTVTAGVAPVSNPRIALVTIVNQPQGD
CCCCCCCCCCCEEEEECCCCCCHHHCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCC
EYYAGQVAAPIFGQVMKDALQILNVAPDESTVKHIDVLN
CEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHEECCC
>Mature Secondary Structure
MKLPNRKKNNKRITKAPPTFIQWRFGVIVFFVFLALAILIARAAYIQVIEPDNLVKQGDM
CCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHCCCCC
RSIRVKSLPSARGIISDRNDEQLAVSVPVDAVWADPVTIYKEGGLAEIERWYALADVLGL
CEEEEECCCCCCCCCCCCCCCEEEEECCCCEEECCCEEEEECCCHHHHHHHHHHHHHHCC
KRKELISKIEKNKKRRFIYLQRQISPAVAAYIKKLKLAGIGLKAESRRYYPTGEISAHVI
HHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEE
GVTGIDSHGLEGVERSYDGWLTGEPGRRTVRKDRYGRVVENISLKEKQEGKPLTLSIDQR
EEECCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCCEEEEHHHH
LQAIAYRAIKQAVSDFRAMSGSVVMIDIKTGEVLAMVNAPSYNPNNREQLQSFRMRNRVI
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHCCCE
TDTLEPGSTVKPFVVLAALDGGTANLDTIIDTGNGIMRIGGSRVRDSSKVGKADLTTILK
EECCCCCCCCCCEEEEEEECCCCCCCCEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHH
KSSNIGVAHLALGMPLQELLAEYAAVGLADPSGLNLIGEATGIFPDRRRWSDFEIATLAF
CCCCCCEEEHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEE
GYGLSVTPIQLAHAYATLGAYGEYRPLSILKTTGPTPGKQVVNRQHAKAVLEMMETVTQP
CCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
GGTATRAAVPGYRVAAKTGTSRKAVRGGYSDEYMTVTAGVAPVSNPRIALVTIVNQPQGD
CCCCCCCCCCCEEEEECCCCCCHHHCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCC
EYYAGQVAAPIFGQVMKDALQILNVAPDESTVKHIDVLN
CEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]