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The map label for this gene is thrC [H]

Identifier: 59712723

GI number: 59712723

Start: 2350413

End: 2351696

Strand: Reverse

Name: thrC [H]

Synonym: VF_2116

Alternate gene names: 59712723

Gene position: 2351696-2350413 (Counterclockwise)

Preceding gene: 59712724

Following gene: 59712721

Centisome position: 81.16

GC content: 38.4

Gene sequence:

>1284_bases
ATGAAGCTGTATAACATAAAAGAAAATGATGAGCAGGTCTCTTTTGGCCAAGCGGTTCGCCAAGGTTTAGGTCGTAATCA
AGGACTATTTTTTCCATCAGAATTACCTAAGTTTGATGATGTTGATGCACTTCTTGCTGAGGATTTTATTTCTCGTAGTA
CTAAAATTTTATCTGCTTTTATCAAAGAGGAACTGCCTCAAGATACCGTAAGAAGTATGGTTGAGAATGCATTCCAATTC
CCTGCACCGATTAAAGCCGTTAAAGATGATGTCTATGCGTTAGAGTTATTTCATGGACCAACATTAGCATTTAAAGATTT
TGGTGGCCGTTTTATGGCCCAATCTTTAGCTGCTGTGACTGATAGCGATGAACAAATTACGATTTTAACAGCAACATCGG
GTGATACTGGTGCGGCTGTTGCTCATGCATTTTATGGTATGGACAATATTAAGGTCGTTATTCTTTATCCGAAAGGTAAA
ATCAGTCCGTTGCAAGAGAAGTTATTTTGTACATTAGGTAAGAATATCCATACCGTTGCAGTTGATGGTGATTTTGATGA
CTGCCAAGCATTAGTGAAACAGTCTTTTGATGATGCAGAACTGCGTAAAGAAGTTGGATTAAACTCTGCAAACTCAATCA
ATATTAGCCGTTTGATGGCACAGATTTGCTACTATTTTGAGGCGGTATCTCAACTACCGAAAGAAAAACGTAATGATGTC
GTTATTTCAGTGCCAAGTGGTAATTTTGGCAACTTAACTGCGGGATTATTGGCGAAATCATTAGGCTTACCTGTAAAACG
CTTTATTGCTGCAACGAATGTTAATGATACCGTTCCTCGTTATCTTGAAACGGGTCAATGGGAACCTAAAGTCACGATCC
CAACGATTTCAAATGCAATGGATGTAAGTCAACCAAATAACTGGCCTCGTATTGAAGAGCTATGTCAGCGTTTAGGATGG
GGATTAGAAACTCTTGGTAAAGGTGCAGTTACTGATGAGCAAAGTGCTGAAACATTAAAAGAGATGAATCAAGCAGGCTA
TCTATGTGAGCCTCATGGTGCCATTGCTTATCGTATATTGAATGAGCAACTCTCTGATGGTGAAACTGGCCTTTTCCTAT
GTACAGCACATCCAGCTAAGTTTAAAGAAGTAGTTGAAGATATTCTAGGCAATGATATTGAGCTACCTGCTCCATTAGCC
AAGCATAATGCGATGGAATTGTTATCTGAAGATTTAGATAATGATTTTGATGCACTTAAAGCAGTATTAAGAAAAGTTCA
ATAA

Upstream 100 bases:

>100_bases
TATCTCGTTGGCTACAAGAAAATTACGTTCAAAATAATGAAGGATTTGTTCATGTTTGTCGTTTAGATAAGCAAGGATCA
CAAGTGACAGGAAGTAAACT

Downstream 100 bases:

>100_bases
GCATTAAGATTGAAATGGATGAGTCTCTTTGGTTAATGATCAAAGGGACTTTTTTATATGACTAAAGTAATAATATCAAT
CGTAGTAAATAATTGAGCAC

Product: threonine synthase

Products: NA

Alternate protein names: TS [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKLYNIKENDEQVSFGQAVRQGLGRNQGLFFPSELPKFDDVDALLAEDFISRSTKILSAFIKEELPQDTVRSMVENAFQF
PAPIKAVKDDVYALELFHGPTLAFKDFGGRFMAQSLAAVTDSDEQITILTATSGDTGAAVAHAFYGMDNIKVVILYPKGK
ISPLQEKLFCTLGKNIHTVAVDGDFDDCQALVKQSFDDAELRKEVGLNSANSINISRLMAQICYYFEAVSQLPKEKRNDV
VISVPSGNFGNLTAGLLAKSLGLPVKRFIAATNVNDTVPRYLETGQWEPKVTIPTISNAMDVSQPNNWPRIEELCQRLGW
GLETLGKGAVTDEQSAETLKEMNQAGYLCEPHGAIAYRILNEQLSDGETGLFLCTAHPAKFKEVVEDILGNDIELPAPLA
KHNAMELLSEDLDNDFDALKAVLRKVQ

Sequences:

>Translated_427_residues
MKLYNIKENDEQVSFGQAVRQGLGRNQGLFFPSELPKFDDVDALLAEDFISRSTKILSAFIKEELPQDTVRSMVENAFQF
PAPIKAVKDDVYALELFHGPTLAFKDFGGRFMAQSLAAVTDSDEQITILTATSGDTGAAVAHAFYGMDNIKVVILYPKGK
ISPLQEKLFCTLGKNIHTVAVDGDFDDCQALVKQSFDDAELRKEVGLNSANSINISRLMAQICYYFEAVSQLPKEKRNDV
VISVPSGNFGNLTAGLLAKSLGLPVKRFIAATNVNDTVPRYLETGQWEPKVTIPTISNAMDVSQPNNWPRIEELCQRLGW
GLETLGKGAVTDEQSAETLKEMNQAGYLCEPHGAIAYRILNEQLSDGETGLFLCTAHPAKFKEVVEDILGNDIELPAPLA
KHNAMELLSEDLDNDFDALKAVLRKVQ
>Mature_427_residues
MKLYNIKENDEQVSFGQAVRQGLGRNQGLFFPSELPKFDDVDALLAEDFISRSTKILSAFIKEELPQDTVRSMVENAFQF
PAPIKAVKDDVYALELFHGPTLAFKDFGGRFMAQSLAAVTDSDEQITILTATSGDTGAAVAHAFYGMDNIKVVILYPKGK
ISPLQEKLFCTLGKNIHTVAVDGDFDDCQALVKQSFDDAELRKEVGLNSANSINISRLMAQICYYFEAVSQLPKEKRNDV
VISVPSGNFGNLTAGLLAKSLGLPVKRFIAATNVNDTVPRYLETGQWEPKVTIPTISNAMDVSQPNNWPRIEELCQRLGW
GLETLGKGAVTDEQSAETLKEMNQAGYLCEPHGAIAYRILNEQLSDGETGLFLCTAHPAKFKEVVEDILGNDIELPAPLA
KHNAMELLSEDLDNDFDALKAVLRKVQ

Specific function: Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine [H]

COG id: COG0498

COG function: function code E; Threonine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine synthase family [H]

Homologues:

Organism=Homo sapiens, GI153792148, Length=430, Percent_Identity=30.6976744186047, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI149193323, Length=435, Percent_Identity=28.2758620689655, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1786185, Length=429, Percent_Identity=67.5990675990676, Blast_Score=583, Evalue=1e-168,
Organism=Saccharomyces cerevisiae, GI6319901, Length=447, Percent_Identity=32.662192393736, Blast_Score=212, Evalue=9e-56,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR004450 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.3.1 [H]

Molecular weight: Translated: 47013; Mature: 47013

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYNIKENDEQVSFGQAVRQGLGRNQGLFFPSELPKFDDVDALLAEDFISRSTKILSAF
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
IKEELPQDTVRSMVENAFQFPAPIKAVKDDVYALELFHGPTLAFKDFGGRFMAQSLAAVT
HHHHCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEEEECCCCEEHHHCCCHHHHHHHHHHC
DSDEQITILTATSGDTGAAVAHAFYGMDNIKVVILYPKGKISPLQEKLFCTLGKNIHTVA
CCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEE
VDGDFDDCQALVKQSFDDAELRKEVGLNSANSINISRLMAQICYYFEAVSQLPKEKRNDV
ECCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
VISVPSGNFGNLTAGLLAKSLGLPVKRFIAATNVNDTVPRYLETGQWEPKVTIPTISNAM
EEECCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHCCCCCCCEECCCCCCCC
DVSQPNNWPRIEELCQRLGWGLETLGKGAVTDEQSAETLKEMNQAGYLCEPHGAIAYRIL
CCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHCCCEECCCCHHHHHHH
NEQLSDGETGLFLCTAHPAKFKEVVEDILGNDIELPAPLAKHNAMELLSEDLDNDFDALK
HHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHH
AVLRKVQ
HHHHHCC
>Mature Secondary Structure
MKLYNIKENDEQVSFGQAVRQGLGRNQGLFFPSELPKFDDVDALLAEDFISRSTKILSAF
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
IKEELPQDTVRSMVENAFQFPAPIKAVKDDVYALELFHGPTLAFKDFGGRFMAQSLAAVT
HHHHCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEEEECCCCEEHHHCCCHHHHHHHHHHC
DSDEQITILTATSGDTGAAVAHAFYGMDNIKVVILYPKGKISPLQEKLFCTLGKNIHTVA
CCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEE
VDGDFDDCQALVKQSFDDAELRKEVGLNSANSINISRLMAQICYYFEAVSQLPKEKRNDV
ECCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
VISVPSGNFGNLTAGLLAKSLGLPVKRFIAATNVNDTVPRYLETGQWEPKVTIPTISNAM
EEECCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHCCCCCCCEECCCCCCCC
DVSQPNNWPRIEELCQRLGWGLETLGKGAVTDEQSAETLKEMNQAGYLCEPHGAIAYRIL
CCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHCCCEECCCCHHHHHHH
NEQLSDGETGLFLCTAHPAKFKEVVEDILGNDIELPAPLAKHNAMELLSEDLDNDFDALK
HHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHH
AVLRKVQ
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]