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The map label for this gene is nfo [H]

Identifier: 59712721

GI number: 59712721

Start: 2348810

End: 2349481

Strand: Reverse

Name: nfo [H]

Synonym: VF_2114

Alternate gene names: 59712721

Gene position: 2349481-2348810 (Counterclockwise)

Preceding gene: 59712723

Following gene: 59712720

Centisome position: 81.09

GC content: 37.65

Gene sequence:

>672_bases
ATGAAATATATTGGTGCGCATGTATCTGCTGCAGGTGGTGTTGATCAAGCGCCAAAACGCGCTTATCAAATTGGTGCTAA
TGCCTTTGCTTTATTTACAAAAAACCAACGTCAATGGGTAGCAAAACCATTAACAAAGAAAATCATCTCTGATTTTAAGG
CTAACTGTAAGCGATTTGGCTTTAGTAGTGAGCAAATACTACCGCATGACTCATACTTAATTAATTTAGGTGCGCCTGAA
ACTGAGAAGCTTGAAAAATCACGAGCGGCTTTTATTGATGAAATGGAGCGCTGCCATCAACTTGGTTTAACGTTATTAAA
TTTTCATCCAGGAAGTCATTTGAAAAAAATTTCAGAAGATGAATGTATATCTTTAATTGCTGAATCGATCAATTTTGCAC
ACCAAGCGGTTCCCGATGTTGTTGCTGTTATTGAAAATACGGCAGGGCAAGGAACCAATTTGGGATGGAAATTTGAACAA
CTGGCTCAAATTATTGAACAGGTCGAAGATAAATCTCGAGTAGGGATTTGCATTGATACTTGCCATACGTTTGCTGCTGG
ATACGATTTAAGTTCAAAAGAAGCGTGTGAACATACTTTCGCTGAATTCGAACGTATTGTTGGTTCTTCAAATTTACGAG
GTATGCATATCAATGATGCAAAATCGAATTAG

Upstream 100 bases:

>100_bases
GATTTATATAACCAATTGTAGGTAGGGCAGAGAAGTACTGACAAATAAAGTGTTTACCGTAATAATAAGACGAAATAAAT
TTTGAAAGGAAATAACAGAG

Downstream 100 bases:

>100_bases
GCAGTCGAGTTGATCGCCATCATTCATTGGGACAAGGAACGATAGGTTGGGATTGCTTTGAATATATTATGCAGGATGAC
CGATTTAATAATATACCGCT

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MKYIGAHVSAAGGVDQAPKRAYQIGANAFALFTKNQRQWVAKPLTKKIISDFKANCKRFGFSSEQILPHDSYLINLGAPE
TEKLEKSRAAFIDEMERCHQLGLTLLNFHPGSHLKKISEDECISLIAESINFAHQAVPDVVAVIENTAGQGTNLGWKFEQ
LAQIIEQVEDKSRVGICIDTCHTFAAGYDLSSKEACEHTFAEFERIVGSSNLRGMHINDAKSN

Sequences:

>Translated_223_residues
MKYIGAHVSAAGGVDQAPKRAYQIGANAFALFTKNQRQWVAKPLTKKIISDFKANCKRFGFSSEQILPHDSYLINLGAPE
TEKLEKSRAAFIDEMERCHQLGLTLLNFHPGSHLKKISEDECISLIAESINFAHQAVPDVVAVIENTAGQGTNLGWKFEQ
LAQIIEQVEDKSRVGICIDTCHTFAAGYDLSSKEACEHTFAEFERIVGSSNLRGMHINDAKSN
>Mature_223_residues
MKYIGAHVSAAGGVDQAPKRAYQIGANAFALFTKNQRQWVAKPLTKKIISDFKANCKRFGFSSEQILPHDSYLINLGAPE
TEKLEKSRAAFIDEMERCHQLGLTLLNFHPGSHLKKISEDECISLIAESINFAHQAVPDVVAVIENTAGQGTNLGWKFEQ
LAQIIEQVEDKSRVGICIDTCHTFAAGYDLSSKEACEHTFAEFERIVGSSNLRGMHINDAKSN

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=223, Percent_Identity=69.5067264573991, Blast_Score=322, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI17531193, Length=222, Percent_Identity=45.945945945946, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6322735, Length=222, Percent_Identity=42.7927927927928, Blast_Score=194, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 24653; Mature: 24653

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIGAHVSAAGGVDQAPKRAYQIGANAFALFTKNQRQWVAKPLTKKIISDFKANCKRFG
CCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
FSSEQILPHDSYLINLGAPETEKLEKSRAAFIDEMERCHQLGLTLLNFHPGSHLKKISED
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHCCHH
ECISLIAESINFAHQAVPDVVAVIENTAGQGTNLGWKFEQLAQIIEQVEDKSRVGICIDT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHH
CHTFAAGYDLSSKEACEHTFAEFERIVGSSNLRGMHINDAKSN
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCC
>Mature Secondary Structure
MKYIGAHVSAAGGVDQAPKRAYQIGANAFALFTKNQRQWVAKPLTKKIISDFKANCKRFG
CCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
FSSEQILPHDSYLINLGAPETEKLEKSRAAFIDEMERCHQLGLTLLNFHPGSHLKKISED
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHCCHH
ECISLIAESINFAHQAVPDVVAVIENTAGQGTNLGWKFEQLAQIIEQVEDKSRVGICIDT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHH
CHTFAAGYDLSSKEACEHTFAEFERIVGSSNLRGMHINDAKSN
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA