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The map label for this gene is ispD

Identifier: 59712680

GI number: 59712680

Start: 2308244

End: 2308960

Strand: Reverse

Name: ispD

Synonym: VF_2073

Alternate gene names: 59712680

Gene position: 2308960-2308244 (Counterclockwise)

Preceding gene: 59712681

Following gene: 59712679

Centisome position: 79.69

GC content: 40.86

Gene sequence:

>717_bases
ATGACTCTTACTACACCTTTGTCGATTACTGCAATTGTTCCTGCCGCAGGGGTAGGAAGCCGAATGAAAGCCGATCGCCC
TAAACAGTATCTATTATTAAATGGAAAAACCGTTTTAGAGCATACCATCGAACAGTTATTAGCTTTCCCTTTAGTTAATA
AAGTCGTTGTTGCTATTACTGATGGTGATCCTTATTTCCCAGAATTAACTCTTGCTCATGATAGCCGAGTGATTCGAGTT
TCTGGCGGTAAAGAGCGTGCGGATTCTGTGTTGTCTGGTCTATCGTACGTTCAAGAGCACCAATTAAGTGAGTGGGTGAT
GGTTCATGATGCTGCTCGCCCTTGTATTCGCCATTCTGACATTGATAAATTAATTGCAGAAGTGATCCCCGAACACATTG
GTGGCATCTTAGCATCGCCAGTTCGAGATACGATGAAACATGCTACTCAAGAACAATGCATTGAAACAACGATTGATCGT
AGCGTGCTGTGGCATGCGTTAACACCACAATTATTTACAACAGAGTTGTTGTTCTCAGCCCTGCAAACAGGGTTAGAGCA
AAATTTATCTATTACTGATGAATCTTCAGCAATTGAGTTAATGGGATATCAACCTAAACTGGTTCAAGGTCGTGCCGATA
ACTTAAAAATCACCCAACCAGAAGATCTCGATTTGGCTGAGTTTTATCTTCAAAAAATGAAAAAGGAAACCAAATAA

Upstream 100 bases:

>100_bases
TTCTGAAGCTATTGAAGAAAGAGCTCGTCATGAATTGGGAATGATAAAAAAAGGCGAAACATTTTATCGAATTATTGATG
AAAATAGTGAGGGGTAATTA

Downstream 100 bases:

>100_bases
TGAGAATTGGACATGGGTTTGATGTACATAAATTTGGCGGCGAAGGCCCAGTCATTATTGGTGGCGTGAGCGTTCCTTAT
GAACAAGGCTTAATTGCTCA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MTLTTPLSITAIVPAAGVGSRMKADRPKQYLLLNGKTVLEHTIEQLLAFPLVNKVVVAITDGDPYFPELTLAHDSRVIRV
SGGKERADSVLSGLSYVQEHQLSEWVMVHDAARPCIRHSDIDKLIAEVIPEHIGGILASPVRDTMKHATQEQCIETTIDR
SVLWHALTPQLFTTELLFSALQTGLEQNLSITDESSAIELMGYQPKLVQGRADNLKITQPEDLDLAEFYLQKMKKETK

Sequences:

>Translated_238_residues
MTLTTPLSITAIVPAAGVGSRMKADRPKQYLLLNGKTVLEHTIEQLLAFPLVNKVVVAITDGDPYFPELTLAHDSRVIRV
SGGKERADSVLSGLSYVQEHQLSEWVMVHDAARPCIRHSDIDKLIAEVIPEHIGGILASPVRDTMKHATQEQCIETTIDR
SVLWHALTPQLFTTELLFSALQTGLEQNLSITDESSAIELMGYQPKLVQGRADNLKITQPEDLDLAEFYLQKMKKETK
>Mature_237_residues
TLTTPLSITAIVPAAGVGSRMKADRPKQYLLLNGKTVLEHTIEQLLAFPLVNKVVVAITDGDPYFPELTLAHDSRVIRVS
GGKERADSVLSGLSYVQEHQLSEWVMVHDAARPCIRHSDIDKLIAEVIPEHIGGILASPVRDTMKHATQEQCIETTIDRS
VLWHALTPQLFTTELLFSALQTGLEQNLSITDESSAIELMGYQPKLVQGRADNLKITQPEDLDLAEFYLQKMKKETK

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=232, Percent_Identity=25.4310344827586, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1789104, Length=230, Percent_Identity=52.1739130434783, Blast_Score=243, Evalue=6e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_VIBF1 (Q5E328)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205456.1
- ProteinModelPortal:   Q5E328
- STRING:   Q5E328
- GeneID:   3279250
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2073
- NMPDR:   fig|312309.3.peg.2070
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   SKVIVVC
- PhylomeDB:   Q5E328
- ProtClustDB:   PRK00155
- BioCyc:   VFIS312309:VF2073-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 26437; Mature: 26306

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTTPLSITAIVPAAGVGSRMKADRPKQYLLLNGKTVLEHTIEQLLAFPLVNKVVVAIT
CCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCEEEEEE
DGDPYFPELTLAHDSRVIRVSGGKERADSVLSGLSYVQEHQLSEWVMVHDAARPCIRHSD
CCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IDKLIAEVIPEHIGGILASPVRDTMKHATQEQCIETTIDRSVLWHALTPQLFTTELLFSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
LQTGLEQNLSITDESSAIELMGYQPKLVQGRADNLKITQPEDLDLAEFYLQKMKKETK
HHHHHHCCCCCCCCCCEEEEECCCCHHHCCCCCCEEECCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLTTPLSITAIVPAAGVGSRMKADRPKQYLLLNGKTVLEHTIEQLLAFPLVNKVVVAIT
CCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCEEEEEE
DGDPYFPELTLAHDSRVIRVSGGKERADSVLSGLSYVQEHQLSEWVMVHDAARPCIRHSD
CCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IDKLIAEVIPEHIGGILASPVRDTMKHATQEQCIETTIDRSVLWHALTPQLFTTELLFSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
LQTGLEQNLSITDESSAIELMGYQPKLVQGRADNLKITQPEDLDLAEFYLQKMKKETK
HHHHHHCCCCCCCCCCEEEEECCCCHHHCCCCCCEEECCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA