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The map label for this gene is ispF [H]

Identifier: 59712679

GI number: 59712679

Start: 2307768

End: 2308244

Strand: Reverse

Name: ispF [H]

Synonym: VF_2072

Alternate gene names: 59712679

Gene position: 2308244-2307768 (Counterclockwise)

Preceding gene: 59712680

Following gene: 59712678

Centisome position: 79.66

GC content: 42.77

Gene sequence:

>477_bases
ATGAGAATTGGACATGGGTTTGATGTACATAAATTTGGCGGCGAAGGCCCAGTCATTATTGGTGGCGTGAGCGTTCCTTA
TGAACAAGGCTTAATTGCTCACTCAGATGGTGATGTTGCTTTGCATGCGTTATGTGATGCGCTACTTGGTGCTATTGCAG
AAGGTGATATTGGGCGTCATTTCCCTGATACTGATGATAAGTGGAAAGGGGCGGATAGCCGTGCTTTACTGCGAGATGTA
TATCGTCGTGTAAAAGAGAAAGGTTTTGCATTGGGAAATGCTGATGTCACTATCATTGCTCAAGCGCCTAAAATGGCTCC
TTATATTCAAGCGATGTGTAATGCAATTGCAGAAGATTTAGAAACAGAGCTAGATAACGTTAATGTAAAAGCCACGACTT
CTGAGCGTTTGGGTTTTACCGGTAGAAAAGAAGGCATTGCTTGTGAAGCAGTAGTGCTGTTAGTTAAAGCATCTTAA

Upstream 100 bases:

>100_bases
AACTGGTTCAAGGTCGTGCCGATAACTTAAAAATCACCCAACCAGAAGATCTCGATTTGGCTGAGTTTTATCTTCAAAAA
ATGAAAAAGGAAACCAAATA

Downstream 100 bases:

>100_bases
GAGATAGATTTATGTCAGACATCATGTCTAATTTTTCGTGGTTATACGGAAAGCCAGTAGCAACAGGTAAATTAAAACAA
CTTCCTGAGCACTTTATTGT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MRIGHGFDVHKFGGEGPVIIGGVSVPYEQGLIAHSDGDVALHALCDALLGAIAEGDIGRHFPDTDDKWKGADSRALLRDV
YRRVKEKGFALGNADVTIIAQAPKMAPYIQAMCNAIAEDLETELDNVNVKATTSERLGFTGRKEGIACEAVVLLVKAS

Sequences:

>Translated_158_residues
MRIGHGFDVHKFGGEGPVIIGGVSVPYEQGLIAHSDGDVALHALCDALLGAIAEGDIGRHFPDTDDKWKGADSRALLRDV
YRRVKEKGFALGNADVTIIAQAPKMAPYIQAMCNAIAEDLETELDNVNVKATTSERLGFTGRKEGIACEAVVLLVKAS
>Mature_158_residues
MRIGHGFDVHKFGGEGPVIIGGVSVPYEQGLIAHSDGDVALHALCDALLGAIAEGDIGRHFPDTDDKWKGADSRALLRDV
YRRVKEKGFALGNADVTIIAQAPKMAPYIQAMCNAIAEDLETELDNVNVKATTSERLGFTGRKEGIACEAVVLLVKAS

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=158, Percent_Identity=73.4177215189873, Blast_Score=214, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 16830; Mature: 16830

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGHGFDVHKFGGEGPVIIGGVSVPYEQGLIAHSDGDVALHALCDALLGAIAEGDIGRH
CCCCCCCCEEEECCCCCEEEECCCCCHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCC
FPDTDDKWKGADSRALLRDVYRRVKEKGFALGNADVTIIAQAPKMAPYIQAMCNAIAEDL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCHHHHHHHHHHHHHH
ETELDNVNVKATTSERLGFTGRKEGIACEAVVLLVKAS
HHHHHCCCEEEECHHHCCCCCCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MRIGHGFDVHKFGGEGPVIIGGVSVPYEQGLIAHSDGDVALHALCDALLGAIAEGDIGRH
CCCCCCCCEEEECCCCCEEEECCCCCHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCC
FPDTDDKWKGADSRALLRDVYRRVKEKGFALGNADVTIIAQAPKMAPYIQAMCNAIAEDL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCHHHHHHHHHHHHHH
ETELDNVNVKATTSERLGFTGRKEGIACEAVVLLVKAS
HHHHHCCCEEEECHHHCCCCCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA