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The map label for this gene is malF2 [H]

Identifier: 59712651

GI number: 59712651

Start: 2274494

End: 2275792

Strand: Direct

Name: malF2 [H]

Synonym: VF_2044

Alternate gene names: 59712651

Gene position: 2274494-2275792 (Clockwise)

Preceding gene: 59712650

Following gene: 59712652

Centisome position: 78.5

GC content: 41.42

Gene sequence:

>1299_bases
ATGAGTCAACAGTTTGTTTTCTGGCTCTCTATGGCAATCCCAGGCAGTGGACACCTTCTACTGCGTCGTATGCGCGAAGC
CGTATTTGGTTTCTCGATGCTGGCCTTAGAGATATTGGTTTATATTCAATTTATTCTACCTAATTTCCGTCTATTAGCTG
TTGAGCAGCCTAATGGAAAAATCACAATTGGTGCTGAGCGCTTTGTTGACGACTCTTTCCTTATTATGGTCGGTTCAACC
ATTGGCGCTTTAATGCTATTGGTCTTTTTAGCTCTACACTATGCGTTTGCTCGTGATGCAAAAGATGTGAAGCGCCAAAT
AGAAGAGTCTGGTTTTGCGATTACCTTTACAGAAAAGGTTCGTGGTTTATCGCACGAAATCGTTCCTAATTTAATTACAG
CACCTAAATTTATTCTTCTGTTCATCTTTGCCTTACTACCTGCAATTGTGTCGATAGTGGTAGCTTTCACCAACTATAAA
CGCCCGATTTTACCTCCAGCGTTTTTAATTGAATGGAAAGGATTAACCAACTTCGAGCGTCTATTTACCAGTGAACGAAC
AGCAGCAGCGTTTCAGGAAACATTAACTTGGACACTGACGTGGACTTTCTGTGCTTCAGCATTAACCATCATCTTAGGTA
CTTTGCTGGCTGTTGTGGCAAACAATAAACACATTCGTGGTAAAAAGTTTTTCCGCACCGTTTACATTTTACCTTGGGCT
GTTCCTGCGTTTTTAACCATCTTAGTTTTCCAACTCTTTTTCTCAAAAGTGGGTGGAATGAATACCATGGTGATCCCTTT
CTTTACGGGTAATGAATATGACATTAGTACCGCTATTGGGTTCTTCCTTAATGCGGACTACGCCAAAATCACCATTATTT
TGATTCAAGCATGGTTGGGATTCCCATACGTGTTTATTTTAGTAACGGGCGTACTACAAACCATCCCAGATGATCTTTAT
GAAGCCAGTGGCATTGATGGTGGTAACGCTTGGACTAACTTCTTTGATATTACTCTGCCATTGATTTTAATCAGTGTTGC
TCCGGTATTCATCACTCAGTTCACCTTCAACTTTAACAACGTCACCATTGTTTACATGCTAGGTGAAGCAACGGTTCAAA
CCGTCGGCTCTATCTACAAACCATTGGATACCATTTCATCTCTTGGTTTCCGCTTAATGATGGATGCGAAATACAACGAA
GCGGCAGTTTACACCTTGATTACCAGTACGGTTGTGGGTGCGGTCGTTCTGTATTCATGGCTAAAAGCGGGCGCATTTAA
AAATGAGGAAGTGATGTAA

Upstream 100 bases:

>100_bases
AACGTACCAACCTCAACGGTGTCCCTATTAAATTAGGGCATCGTTGTCCTCTAAATCCGTTACATATCTACACTAACTTA
ATAAAAAAGTAGGTTATAAC

Downstream 100 bases:

>100_bases
TGGATAAGGCAATCAATCTTAATCATCAAACAAAAGCAACTAAGTTCAATCGCCATGTCCTCACAAAAAACCTAGGGCTA
TTCTTTAGCTATGCGTTTTT

Product: maltose transport system permease protein MalF

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSQQFVFWLSMAIPGSGHLLLRRMREAVFGFSMLALEILVYIQFILPNFRLLAVEQPNGKITIGAERFVDDSFLIMVGST
IGALMLLVFLALHYAFARDAKDVKRQIEESGFAITFTEKVRGLSHEIVPNLITAPKFILLFIFALLPAIVSIVVAFTNYK
RPILPPAFLIEWKGLTNFERLFTSERTAAAFQETLTWTLTWTFCASALTIILGTLLAVVANNKHIRGKKFFRTVYILPWA
VPAFLTILVFQLFFSKVGGMNTMVIPFFTGNEYDISTAIGFFLNADYAKITIILIQAWLGFPYVFILVTGVLQTIPDDLY
EASGIDGGNAWTNFFDITLPLILISVAPVFITQFTFNFNNVTIVYMLGEATVQTVGSIYKPLDTISSLGFRLMMDAKYNE
AAVYTLITSTVVGAVVLYSWLKAGAFKNEEVM

Sequences:

>Translated_432_residues
MSQQFVFWLSMAIPGSGHLLLRRMREAVFGFSMLALEILVYIQFILPNFRLLAVEQPNGKITIGAERFVDDSFLIMVGST
IGALMLLVFLALHYAFARDAKDVKRQIEESGFAITFTEKVRGLSHEIVPNLITAPKFILLFIFALLPAIVSIVVAFTNYK
RPILPPAFLIEWKGLTNFERLFTSERTAAAFQETLTWTLTWTFCASALTIILGTLLAVVANNKHIRGKKFFRTVYILPWA
VPAFLTILVFQLFFSKVGGMNTMVIPFFTGNEYDISTAIGFFLNADYAKITIILIQAWLGFPYVFILVTGVLQTIPDDLY
EASGIDGGNAWTNFFDITLPLILISVAPVFITQFTFNFNNVTIVYMLGEATVQTVGSIYKPLDTISSLGFRLMMDAKYNE
AAVYTLITSTVVGAVVLYSWLKAGAFKNEEVM
>Mature_431_residues
SQQFVFWLSMAIPGSGHLLLRRMREAVFGFSMLALEILVYIQFILPNFRLLAVEQPNGKITIGAERFVDDSFLIMVGSTI
GALMLLVFLALHYAFARDAKDVKRQIEESGFAITFTEKVRGLSHEIVPNLITAPKFILLFIFALLPAIVSIVVAFTNYKR
PILPPAFLIEWKGLTNFERLFTSERTAAAFQETLTWTLTWTFCASALTIILGTLLAVVANNKHIRGKKFFRTVYILPWAV
PAFLTILVFQLFFSKVGGMNTMVIPFFTGNEYDISTAIGFFLNADYAKITIILIQAWLGFPYVFILVTGVLQTIPDDLYE
ASGIDGGNAWTNFFDITLPLILISVAPVFITQFTFNFNNVTIVYMLGEATVQTVGSIYKPLDTISSLGFRLMMDAKYNEA
AVYTLITSTVVGAVVLYSWLKAGAFKNEEVM

Specific function: Part of the binding-protein-dependent transport system for maltodextrin; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790465, Length=226, Percent_Identity=35.3982300884956, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1787570, Length=218, Percent_Identity=28.4403669724771, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789861, Length=222, Percent_Identity=27.4774774774775, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR007829 [H]

Pfam domain/function: PF00528 BPD_transp_1; PF05154 TM2 [H]

EC number: NA

Molecular weight: Translated: 48362; Mature: 48231

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQFVFWLSMAIPGSGHLLLRRMREAVFGFSMLALEILVYIQFILPNFRLLAVEQPNGK
CCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
ITIGAERFVDDSFLIMVGSTIGALMLLVFLALHYAFARDAKDVKRQIEESGFAITFTEKV
EEECHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEHHHH
RGLSHEIVPNLITAPKFILLFIFALLPAIVSIVVAFTNYKRPILPPAFLIEWKGLTNFER
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHH
LFTSERTAAAFQETLTWTLTWTFCASALTIILGTLLAVVANNKHIRGKKFFRTVYILPWA
HHHCHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VPAFLTILVFQLFFSKVGGMNTMVIPFFTGNEYDISTAIGFFLNADYAKITIILIQAWLG
HHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEHHHHHHHHHCCCHHHHHHHHHHHHHH
FPYVFILVTGVLQTIPDDLYEASGIDGGNAWTNFFDITLPLILISVAPVFITQFTFNFNN
HHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCC
VTIVYMLGEATVQTVGSIYKPLDTISSLGFRLMMDAKYNEAAVYTLITSTVVGAVVLYSW
EEEEEEECHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
LKAGAFKNEEVM
HHHCCCCCCCCC
>Mature Secondary Structure 
SQQFVFWLSMAIPGSGHLLLRRMREAVFGFSMLALEILVYIQFILPNFRLLAVEQPNGK
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
ITIGAERFVDDSFLIMVGSTIGALMLLVFLALHYAFARDAKDVKRQIEESGFAITFTEKV
EEECHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEHHHH
RGLSHEIVPNLITAPKFILLFIFALLPAIVSIVVAFTNYKRPILPPAFLIEWKGLTNFER
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHH
LFTSERTAAAFQETLTWTLTWTFCASALTIILGTLLAVVANNKHIRGKKFFRTVYILPWA
HHHCHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VPAFLTILVFQLFFSKVGGMNTMVIPFFTGNEYDISTAIGFFLNADYAKITIILIQAWLG
HHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEHHHHHHHHHCCCHHHHHHHHHHHHHH
FPYVFILVTGVLQTIPDDLYEASGIDGGNAWTNFFDITLPLILISVAPVFITQFTFNFNN
HHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCC
VTIVYMLGEATVQTVGSIYKPLDTISSLGFRLMMDAKYNEAAVYTLITSTVVGAVVLYSW
EEEEEEECHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
LKAGAFKNEEVM
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8478935; 11463916 [H]