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The map label for this gene is grpE

Identifier: 59712603

GI number: 59712603

Start: 2231758

End: 2232342

Strand: Reverse

Name: grpE

Synonym: VF_1996

Alternate gene names: 59712603

Gene position: 2232342-2231758 (Counterclockwise)

Preceding gene: 59712607

Following gene: 59712602

Centisome position: 77.04

GC content: 40.68

Gene sequence:

>585_bases
ATGAGTAACGAAGAAAACAAAGTAAACGAAGAAGCAGTAGTAGAAGAGCAAGTTGAAGCAGTTGGCACAGAAGCTGATGT
GGAATGGAATGAATCTGCTGAAGATAGCCAAGAAGCAAAAATTGCAGAACTAGAAGCAGCACTACTTGCTAGCCAAGCAC
AAGTTAAAGAACAACAAGATACGGTTCTACGAGCAAAAGCTGAAGAGCAAAATGTTCGTCGTCGTGCTGAAGAAGACGTT
GATAAAGCGCGTAAATACGCACTGAAGAAATTTGCTGGTGAGCTTCTTCCTGTTCTAGATAACTTAGAGCGTGCTCTTGA
AAGCGGTGATAAAGAAAATGAAGCTGCAAAAGCATTGCTTGAAGGTGTTGAACTAACACTTCAAACGTTTGTATCGACGG
TTGAGAAATTCGGCTTAACGGTAATTAACCCAATGGGTGAAGCGTTTAACCCTGAACTTCATCAAGCAATTGGCATGCAA
GCAAGCCCAGATCATGAATCAAATACAGTTATGATCGTGATGCAAAAAGGCTACACATTAAATGATCAAGTATTACGTCC
AGCAATGGTAATGGTTGCTCAATAA

Upstream 100 bases:

>100_bases
ATAATAATGTCAAAGTTTTAATTTGCGTATAGAGCGACGCCTTGGTTTGTATTAAGATCGCTTTATATAGCTAGTCATTA
GCATTCTGGAGAAAGCAGTA

Downstream 100 bases:

>100_bases
TCATCATTTTTTGATTCAAATTATGATTAAAAATCAAGCGGTTCCGAGTTCGGGATCGCTTTTTTTTGTCTTTTTTTTAA
GATGTAATGTCTCTGTTTTT

Product: heat shock protein

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MSNEENKVNEEAVVEEQVEAVGTEADVEWNESAEDSQEAKIAELEAALLASQAQVKEQQDTVLRAKAEEQNVRRRAEEDV
DKARKYALKKFAGELLPVLDNLERALESGDKENEAAKALLEGVELTLQTFVSTVEKFGLTVINPMGEAFNPELHQAIGMQ
ASPDHESNTVMIVMQKGYTLNDQVLRPAMVMVAQ

Sequences:

>Translated_194_residues
MSNEENKVNEEAVVEEQVEAVGTEADVEWNESAEDSQEAKIAELEAALLASQAQVKEQQDTVLRAKAEEQNVRRRAEEDV
DKARKYALKKFAGELLPVLDNLERALESGDKENEAAKALLEGVELTLQTFVSTVEKFGLTVINPMGEAFNPELHQAIGMQ
ASPDHESNTVMIVMQKGYTLNDQVLRPAMVMVAQ
>Mature_193_residues
SNEENKVNEEAVVEEQVEAVGTEADVEWNESAEDSQEAKIAELEAALLASQAQVKEQQDTVLRAKAEEQNVRRRAEEDVD
KARKYALKKFAGELLPVLDNLERALESGDKENEAAKALLEGVELTLQTFVSTVEKFGLTVINPMGEAFNPELHQAIGMQA
SPDHESNTVMIVMQKGYTLNDQVLRPAMVMVAQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=146, Percent_Identity=34.9315068493151, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI40255109, Length=148, Percent_Identity=31.0810810810811, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1788967, Length=192, Percent_Identity=46.3541666666667, Blast_Score=153, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI17552458, Length=151, Percent_Identity=35.0993377483444, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324806, Length=197, Percent_Identity=35.0253807106599, Blast_Score=108, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24653432, Length=158, Percent_Identity=29.1139240506329, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_VIBF1 (Q5E3A5)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205379.1
- ProteinModelPortal:   Q5E3A5
- SMR:   Q5E3A5
- STRING:   Q5E3A5
- GeneID:   3279133
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1996
- NMPDR:   fig|312309.3.peg.1996
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- PhylomeDB:   Q5E3A5
- ProtClustDB:   CLSK2396207
- BioCyc:   VFIS312309:VF1996-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21470; Mature: 21339

Theoretical pI: Translated: 4.13; Mature: 4.13

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEENKVNEEAVVEEQVEAVGTEADVEWNESAEDSQEAKIAELEAALLASQAQVKEQQD
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TVLRAKAEEQNVRRRAEEDVDKARKYALKKFAGELLPVLDNLERALESGDKENEAAKALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EGVELTLQTFVSTVEKFGLTVINPMGEAFNPELHQAIGMQASPDHESNTVMIVMQKGYTL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCC
NDQVLRPAMVMVAQ
CHHHHHHHHHHHCC
>Mature Secondary Structure 
SNEENKVNEEAVVEEQVEAVGTEADVEWNESAEDSQEAKIAELEAALLASQAQVKEQQD
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TVLRAKAEEQNVRRRAEEDVDKARKYALKKFAGELLPVLDNLERALESGDKENEAAKALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EGVELTLQTFVSTVEKFGLTVINPMGEAFNPELHQAIGMQASPDHESNTVMIVMQKGYTL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCC
NDQVLRPAMVMVAQ
CHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA