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The map label for this gene is aceB [H]

Identifier: 59712580

GI number: 59712580

Start: 2204974

End: 2206578

Strand: Reverse

Name: aceB [H]

Synonym: VF_1973

Alternate gene names: 59712580

Gene position: 2206578-2204974 (Counterclockwise)

Preceding gene: 59712581

Following gene: 59712579

Centisome position: 76.15

GC content: 40.56

Gene sequence:

>1605_bases
ATGACTGATACCATGACAATTAAAGAACAACAAATAACCATTACTAAACCGCATTTGCCTCAAGTTGAAGAGGTGTTAAC
TGCAGGTGCTGTTCGATTTCTTACTCATGTTGTCGAAAAATTTGCACACCGGATTCCTGAGCTTTTAGAAACTCGTCAGC
TGCGCCAGAAAAAAATAGACGCAGGGAAATTGCCTAATTTCTTAAGTGAAACGCGTTCTATTCGAGAATCAGAGTGGAGT
ATCCAAGGTATTCCTGCAGATTTACAAGATCGCCGAGTAGAAATTACTGGCCCAACAGATAGAAAAATGGTGATTAACGC
ACTTAATGCCAATGTAAAAGTGTTTATGGCAGATTTTGAAGATTCATTAGCACCATCATGGGAGGCTGTACTTAATGGTC
AGATTAATCTAAGAGATGCCATGAATGGCACGATTCAATATACCAACCCCAAAACTCAAAAAGTGTATCAACTAAAGCAA
GATCCTGCGGTATTGATTTGTCGAGTTCGCGGTTTGCACCTTCCTGAAAAGCACGTGCTATTTAATGGTGTTGCAATACC
TGGAGCTTTGTTTGATTTTGCTCTTTATTTTTATCACAACTATCAAACGTCTTTAAAAAAAGGCAGCGGCCCATATTTTT
ATTTACCTAAGTTGCAGGCGTATCAAGAAGCGGCGTGGTGGAGTGATGTGTTCAGTTTTGCTGAACGTGAATTCGGATTA
AAGCAGGGAACAATTAAAGCCACTGTTCTTATTGAAACATTACCTGCTGTATTTGAGATGGATGAAATTCTATATAGCTT
AAAAGAGCATATTGTTGGACTGAATTGTGGACGTTGGGATTATATCTTCAGCTACATCAAAACATTAAAAAATCACCCAG
ACCGTGTATTACCGGATCGCCAAGTGGTGACCATGGAAAAACCGTTTCTCAATGCGTATTCACGATTACTCATTCGAACA
TGCCACAGAAGAGGAGCATTTGCGATGGGGGGAATGGCCGCCTTTATCCCATCAAAAGATCCAGTAAAAAATGACTGGGT
ACTAAATAAAATTCAAACCGATAAATCATTAGAAGCCGGTAACGGACATGATGGTACTTGGGTCGCTCATCCTGGCTTAG
CTGATACGGCAAGAGTAGAGTTTGATTCAGTATTAGGTGAACGAATGAATCAACTGGATATTACACGAGATACTGATGCG
CCAATTACTGCAGAGGAATTACTGGCACCATGTGAAGGAGAAAGAACAGAAGAAGGAATGCGTCATAACATTCGAGTTGC
AGTGCAATACATTGAAGCTTGGATATCAGGAAATGGCTGCGTGCCTATTTACGGATTAATGGAAGATGCGGCTACCGCAG
AAATATCACGAACGTCTATTTGGCAATGGATACAACATGGCAAAACACTAGATAGTGGTGCAGTTGTTACTGCTGAACTA
TTCAGAACCATGTTACTGGAAGAGATGAAAGTAATAGAGAAAGAGTTGGGAACAGAGCGTTTTGATTCTGGCCAATTTAC
TCAAGCTGCAAAGCTGATGGAGCAACTAACCACCAGTGATCAACTTACGGACTTTTTAACACTACCTGGCTACCAATACC
TATAA

Upstream 100 bases:

>100_bases
TGTAATAAGCGTAAGCAAGAAAAGTAACCCACAGGATGATACATGAATACTAAATCGTAGTGTTTTAGTTACAAATGTAA
TTTTGCCGGAGGCGCAAACT

Downstream 100 bases:

>100_bases
GAGAGGGAACGATTATGACGACATTTAAACCACAAACTCGCCAACAGCAAATTGATGCACTGGAAAAAGATTGGGCTGAA
AATCCGCGTTGGGATGGGGT

Product: malate synthase

Products: NA

Alternate protein names: MSA [H]

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MTDTMTIKEQQITITKPHLPQVEEVLTAGAVRFLTHVVEKFAHRIPELLETRQLRQKKIDAGKLPNFLSETRSIRESEWS
IQGIPADLQDRRVEITGPTDRKMVINALNANVKVFMADFEDSLAPSWEAVLNGQINLRDAMNGTIQYTNPKTQKVYQLKQ
DPAVLICRVRGLHLPEKHVLFNGVAIPGALFDFALYFYHNYQTSLKKGSGPYFYLPKLQAYQEAAWWSDVFSFAEREFGL
KQGTIKATVLIETLPAVFEMDEILYSLKEHIVGLNCGRWDYIFSYIKTLKNHPDRVLPDRQVVTMEKPFLNAYSRLLIRT
CHRRGAFAMGGMAAFIPSKDPVKNDWVLNKIQTDKSLEAGNGHDGTWVAHPGLADTARVEFDSVLGERMNQLDITRDTDA
PITAEELLAPCEGERTEEGMRHNIRVAVQYIEAWISGNGCVPIYGLMEDAATAEISRTSIWQWIQHGKTLDSGAVVTAEL
FRTMLLEEMKVIEKELGTERFDSGQFTQAAKLMEQLTTSDQLTDFLTLPGYQYL

Sequences:

>Translated_534_residues
MTDTMTIKEQQITITKPHLPQVEEVLTAGAVRFLTHVVEKFAHRIPELLETRQLRQKKIDAGKLPNFLSETRSIRESEWS
IQGIPADLQDRRVEITGPTDRKMVINALNANVKVFMADFEDSLAPSWEAVLNGQINLRDAMNGTIQYTNPKTQKVYQLKQ
DPAVLICRVRGLHLPEKHVLFNGVAIPGALFDFALYFYHNYQTSLKKGSGPYFYLPKLQAYQEAAWWSDVFSFAEREFGL
KQGTIKATVLIETLPAVFEMDEILYSLKEHIVGLNCGRWDYIFSYIKTLKNHPDRVLPDRQVVTMEKPFLNAYSRLLIRT
CHRRGAFAMGGMAAFIPSKDPVKNDWVLNKIQTDKSLEAGNGHDGTWVAHPGLADTARVEFDSVLGERMNQLDITRDTDA
PITAEELLAPCEGERTEEGMRHNIRVAVQYIEAWISGNGCVPIYGLMEDAATAEISRTSIWQWIQHGKTLDSGAVVTAEL
FRTMLLEEMKVIEKELGTERFDSGQFTQAAKLMEQLTTSDQLTDFLTLPGYQYL
>Mature_533_residues
TDTMTIKEQQITITKPHLPQVEEVLTAGAVRFLTHVVEKFAHRIPELLETRQLRQKKIDAGKLPNFLSETRSIRESEWSI
QGIPADLQDRRVEITGPTDRKMVINALNANVKVFMADFEDSLAPSWEAVLNGQINLRDAMNGTIQYTNPKTQKVYQLKQD
PAVLICRVRGLHLPEKHVLFNGVAIPGALFDFALYFYHNYQTSLKKGSGPYFYLPKLQAYQEAAWWSDVFSFAEREFGLK
QGTIKATVLIETLPAVFEMDEILYSLKEHIVGLNCGRWDYIFSYIKTLKNHPDRVLPDRQVVTMEKPFLNAYSRLLIRTC
HRRGAFAMGGMAAFIPSKDPVKNDWVLNKIQTDKSLEAGNGHDGTWVAHPGLADTARVEFDSVLGERMNQLDITRDTDAP
ITAEELLAPCEGERTEEGMRHNIRVAVQYIEAWISGNGCVPIYGLMEDAATAEISRTSIWQWIQHGKTLDSGAVVTAELF
RTMLLEEMKVIEKELGTERFDSGQFTQAAKLMEQLTTSDQLTDFLTLPGYQYL

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=534, Percent_Identity=72.6591760299626, Blast_Score=818, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17561814, Length=531, Percent_Identity=48.9642184557439, Blast_Score=498, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI71982926, Length=425, Percent_Identity=51.2941176470588, Blast_Score=413, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324212, Length=521, Percent_Identity=45.873320537428, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6322222, Length=521, Percent_Identity=46.0652591170825, Blast_Score=442, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 60580; Mature: 60449

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTMTIKEQQITITKPHLPQVEEVLTAGAVRFLTHVVEKFAHRIPELLETRQLRQKKID
CCCCEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AGKLPNFLSETRSIRESEWSIQGIPADLQDRRVEITGPTDRKMVINALNANVKVFMADFE
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHEECCCEEEEEECCC
DSLAPSWEAVLNGQINLRDAMNGTIQYTNPKTQKVYQLKQDPAVLICRVRGLHLPEKHVL
CCCCCCHHHHHCCCEEEHHCCCCCEEECCCCHHHHHHHCCCCEEEEEEECCCCCCCHHHE
FNGVAIPGALFDFALYFYHNYQTSLKKGSGPYFYLPKLQAYQEAAWWSDVFSFAEREFGL
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCC
KQGTIKATVLIETLPAVFEMDEILYSLKEHIVGLNCGRWDYIFSYIKTLKNHPDRVLPDR
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
QVVTMEKPFLNAYSRLLIRTCHRRGAFAMGGMAAFIPSKDPVKNDWVLNKIQTDKSLEAG
EEEEECCHHHHHHHHHHHHHHHHCCCCHHCCEEEECCCCCCCCCCHHHHHHCCCCCCCCC
NGHDGTWVAHPGLADTARVEFDSVLGERMNQLDITRDTDAPITAEELLAPCEGERTEEGM
CCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCHHHH
RHNIRVAVQYIEAWISGNGCVPIYGLMEDAATAEISRTSIWQWIQHGKTLDSGAVVTAEL
HHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
FRTMLLEEMKVIEKELGTERFDSGQFTQAAKLMEQLTTSDQLTDFLTLPGYQYL
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TDTMTIKEQQITITKPHLPQVEEVLTAGAVRFLTHVVEKFAHRIPELLETRQLRQKKID
CCCEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AGKLPNFLSETRSIRESEWSIQGIPADLQDRRVEITGPTDRKMVINALNANVKVFMADFE
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHEECCCEEEEEECCC
DSLAPSWEAVLNGQINLRDAMNGTIQYTNPKTQKVYQLKQDPAVLICRVRGLHLPEKHVL
CCCCCCHHHHHCCCEEEHHCCCCCEEECCCCHHHHHHHCCCCEEEEEEECCCCCCCHHHE
FNGVAIPGALFDFALYFYHNYQTSLKKGSGPYFYLPKLQAYQEAAWWSDVFSFAEREFGL
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCC
KQGTIKATVLIETLPAVFEMDEILYSLKEHIVGLNCGRWDYIFSYIKTLKNHPDRVLPDR
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
QVVTMEKPFLNAYSRLLIRTCHRRGAFAMGGMAAFIPSKDPVKNDWVLNKIQTDKSLEAG
EEEEECCHHHHHHHHHHHHHHHHCCCCHHCCEEEECCCCCCCCCCHHHHHHCCCCCCCCC
NGHDGTWVAHPGLADTARVEFDSVLGERMNQLDITRDTDAPITAEELLAPCEGERTEEGM
CCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCHHHH
RHNIRVAVQYIEAWISGNGCVPIYGLMEDAATAEISRTSIWQWIQHGKTLDSGAVVTAEL
HHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
FRTMLLEEMKVIEKELGTERFDSGQFTQAAKLMEQLTTSDQLTDFLTLPGYQYL
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3050899; 3060852; 8265357; 9278503 [H]