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The map label for this gene is tsf

Identifier: 59712568

GI number: 59712568

Start: 2191015

End: 2191860

Strand: Reverse

Name: tsf

Synonym: VF_1961

Alternate gene names: 59712568

Gene position: 2191860-2191015 (Counterclockwise)

Preceding gene: 59712569

Following gene: 59712567

Centisome position: 75.65

GC content: 43.62

Gene sequence:

>846_bases
ATGGCAACTGTAACTGCTGCTCTTGTAAAAGAACTTCGTGAGCGCACTGGCGCTGGCATGATGGAATGTAAAAAAGCGCT
AGTTGAAGCGAACGCTGATATCGAACTAGCGATCGAAAACATGCGTAAATCTGGCGCTGCTAAAGCTGCTAAAAAAGCAG
GTAACGTTGCTGCTGAAGGCGCAATCATCATCAAAGAAGAAAACGGCGTTGCTGCTCTTCTTGAAGTTAACTGTCAAACT
GATTTCGTTGCTAAAGATTCTAACTTCACAGCATTTGCTGAAGAAGTTGTTGCTGCAGCTGTTGCAACTCAAGCAACTGT
TGAAGAACTTCAAGCACAATTCGAAGAAACTCGTGTTGCTCTAGTAGCTAAAATCGGTGAAAACATCAACATCCGTCGCG
TACAATACGTTGCTGGTTCTGCACTAGCTTCTTACCGTCACGGCGAGAAAATCGGTGTTGTTGTAGCTGGTGAAGGCGAT
GCTGAAACTCTTAAGCACATCGCTATGCACGTTGCTGCTTCTAAGCCTGAGTACGTTAACCCATCTGACGTACCTGCTGA
CGTAGTAGAAAAAGAAAAAGCTGTTCAAGTTGAAATCGCTATGAACGAAGGCAAACCTCAAGAAATCGCTGAGAAAATGG
TTATCGGCCGTATGAAGAAATTCACGGGCGAAGTTTCTCTAACAGGTCAAGCTTTCATCATGGAACCTAAGAAAACTGTT
GCTGAAATTCTTAAAGAGAAAGGCGCTTCAGTATCTAACTTCGTACGTCTAGAAGTTGGTGAAGGTATCGAGAAAGCTGC
AGAAATGAGCTTTGCTGATGAAGTAGCTGCAGTACAAAAAGGTTAA

Upstream 100 bases:

>100_bases
CCTAGTGAGTCTTTTCGTTTCGTAGTAAACTGAAATAGTTAGCAGGGGCCCAATTTGGCCCCTGATTTTTAACTCCACCA
ATTAAATTGAGGAATTGAGA

Downstream 100 bases:

>100_bases
TTCCTTCTGTATTGTAACTGAAAGAGACCGTGGCTATGCTGCGGTCTTTTTGTGAATAGCTGTTACCTTTTACTATTGAA
TTATCAATAGAGGATAATCC

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MATVTAALVKELRERTGAGMMECKKALVEANADIELAIENMRKSGAAKAAKKAGNVAAEGAIIIKEENGVAALLEVNCQT
DFVAKDSNFTAFAEEVVAAAVATQATVEELQAQFEETRVALVAKIGENINIRRVQYVAGSALASYRHGEKIGVVVAGEGD
AETLKHIAMHVAASKPEYVNPSDVPADVVEKEKAVQVEIAMNEGKPQEIAEKMVIGRMKKFTGEVSLTGQAFIMEPKKTV
AEILKEKGASVSNFVRLEVGEGIEKAAEMSFADEVAAVQKG

Sequences:

>Translated_281_residues
MATVTAALVKELRERTGAGMMECKKALVEANADIELAIENMRKSGAAKAAKKAGNVAAEGAIIIKEENGVAALLEVNCQT
DFVAKDSNFTAFAEEVVAAAVATQATVEELQAQFEETRVALVAKIGENINIRRVQYVAGSALASYRHGEKIGVVVAGEGD
AETLKHIAMHVAASKPEYVNPSDVPADVVEKEKAVQVEIAMNEGKPQEIAEKMVIGRMKKFTGEVSLTGQAFIMEPKKTV
AEILKEKGASVSNFVRLEVGEGIEKAAEMSFADEVAAVQKG
>Mature_280_residues
ATVTAALVKELRERTGAGMMECKKALVEANADIELAIENMRKSGAAKAAKKAGNVAAEGAIIIKEENGVAALLEVNCQTD
FVAKDSNFTAFAEEVVAAAVATQATVEELQAQFEETRVALVAKIGENINIRRVQYVAGSALASYRHGEKIGVVVAGEGDA
ETLKHIAMHVAASKPEYVNPSDVPADVVEKEKAVQVEIAMNEGKPQEIAEKMVIGRMKKFTGEVSLTGQAFIMEPKKTVA
EILKEKGASVSNFVRLEVGEGIEKAAEMSFADEVAAVQKG

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Homo sapiens, GI171846268, Length=287, Percent_Identity=24.0418118466899, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI291084500, Length=308, Percent_Identity=22.7272727272727, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI291084498, Length=110, Percent_Identity=34.5454545454545, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1786366, Length=282, Percent_Identity=70.2127659574468, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17561440, Length=292, Percent_Identity=25, Blast_Score=85, Evalue=3e-17,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_VIBF1 (Q5E3E0)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205344.1
- ProteinModelPortal:   Q5E3E0
- SMR:   Q5E3E0
- STRING:   Q5E3E0
- GeneID:   3279217
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1961
- NMPDR:   fig|312309.3.peg.1961
- eggNOG:   COG0264
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- PhylomeDB:   Q5E3E0
- ProtClustDB:   PRK09377
- BioCyc:   VFIS312309:VF1961-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 29834; Mature: 29702

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVTAALVKELRERTGAGMMECKKALVEANADIELAIENMRKSGAAKAAKKAGNVAAEG
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCEEECC
AIIIKEENGVAALLEVNCQTDFVAKDSNFTAFAEEVVAAAVATQATVEELQAQFEETRVA
EEEEEECCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVAKIGENINIRRVQYVAGSALASYRHGEKIGVVVAGEGDAETLKHIAMHVAASKPEYVN
HHHHHCCCCCEEEEEHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCC
PSDVPADVVEKEKAVQVEIAMNEGKPQEIAEKMVIGRMKKFTGEVSLTGQAFIMEPKKTV
CCCCCHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEECCCEEEECCHHHH
AEILKEKGASVSNFVRLEVGEGIEKAAEMSFADEVAAVQKG
HHHHHHCCCCHHHEEEEECCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ATVTAALVKELRERTGAGMMECKKALVEANADIELAIENMRKSGAAKAAKKAGNVAAEG
CCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCEEECC
AIIIKEENGVAALLEVNCQTDFVAKDSNFTAFAEEVVAAAVATQATVEELQAQFEETRVA
EEEEEECCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVAKIGENINIRRVQYVAGSALASYRHGEKIGVVVAGEGDAETLKHIAMHVAASKPEYVN
HHHHHCCCCCEEEEEHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCC
PSDVPADVVEKEKAVQVEIAMNEGKPQEIAEKMVIGRMKKFTGEVSLTGQAFIMEPKKTV
CCCCCHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEECCCEEEECCHHHH
AEILKEKGASVSNFVRLEVGEGIEKAAEMSFADEVAAVQKG
HHHHHHCCCCHHHEEEEECCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA