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The map label for this gene is lpxD

Identifier: 59712559

GI number: 59712559

Start: 2181053

End: 2182072

Strand: Reverse

Name: lpxD

Synonym: VF_1952

Alternate gene names: 59712559

Gene position: 2182072-2181053 (Counterclockwise)

Preceding gene: 59712560

Following gene: 59712558

Centisome position: 75.31

GC content: 41.27

Gene sequence:

>1020_bases
ATGTCTCGTATTACGCTTGCTGAATTGGCAGAAAAATTAGATGCAACAGTCCATGGTGATGGCACTGTTGTGGTTCACTC
AATCGCTTCGATGAGCAAAGCAAAGGAAGGAGACATTACTTTCCTTTCGGATGCTAAGTATCGTAAACATTTAGTTGAGT
GCCAAGCTTCTGCTGTTATTGTTAAAGAAGCAGATGTGGAATTCTGTCAATCTAACGTTCTAATAATGCGAGACCCATAT
TTAGGCTTTGCACTAGCAGCACAAGCATTAGACACGACACCAGCTCCTGCTAGTGATATTGCACCTTCTGCTTATATTGC
AGATGACGCGATTATCGGTGAAGGTGTTGCTATTGGTCATAATGCGGTTATTGAATCAAAAGCGGTTATTGCTGATGGTG
CAATGATTGGTGCTGGTTGTTTTATCGGTAAAGAAGCAAAAATCGGTAAAAATACTAAGCTATGGGCAAATGTGTCTGTT
TATCACCGTGTTGAAATTGGCGAAGCTTGCCTTGTTCAATCTGGAACAGTTATTGGTTCTGATGGCTTTGGCTATGCAAA
TGATCGTGGTACTTGGGTTAAGATCCCTCAGCTAGGCTCTGTAATTATTGGCGATAACGTTGAGATTGGTGCCAATACGA
CGATTGATCGTGGTGCTATTGATGACACGGTTATTGAAAGCAATGTTATCATTGATAACCAAATTCAAATTGCACATAAC
GTACAAATCGGATCTGGTTCAGCAATGGCTGGTGGTACTATAGTTGCAGGCAGTACTAAAATTGGTAAGCACTGTATTAT
TGGCGGTGGCTCTGTAATTAATGGCCATATCGAAATTACGGATGGGGTAACCATCACAGGTATGGGCATGGTAATGCGAG
CGATTGATGAGAAAGGTATGTACTCATCAGGCATTCCACTGCAAACCAATAAAGAATGGCGTAAAACAGCTGCTCGCGTT
CATAAGATTGATGAAATGAACAAGCGATTAAAAGCAGTTGAGAAAAAGTTAGTCGATTAG

Upstream 100 bases:

>100_bases
AAGGCTACGATATGGTTGTTGACGCTGCAACATTGCTTTGGGCTAAACCAGAAGACAACCTATCAGAAAAAGTTATTAAA
GCAATTAAATAAGGTTGAAT

Downstream 100 bases:

>100_bases
TATTAACTTCACTACATTCTAATAAATAAGTATTGGTCTGCATTTTGCAGACCTTTTTCTATCTATTATTACTCTATTTG
AGCTACACTATTGCTCAGAT

Product: UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Products: UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine; [Acyl-Carrier Protein]. [C]

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MSRITLAELAEKLDATVHGDGTVVVHSIASMSKAKEGDITFLSDAKYRKHLVECQASAVIVKEADVEFCQSNVLIMRDPY
LGFALAAQALDTTPAPASDIAPSAYIADDAIIGEGVAIGHNAVIESKAVIADGAMIGAGCFIGKEAKIGKNTKLWANVSV
YHRVEIGEACLVQSGTVIGSDGFGYANDRGTWVKIPQLGSVIIGDNVEIGANTTIDRGAIDDTVIESNVIIDNQIQIAHN
VQIGSGSAMAGGTIVAGSTKIGKHCIIGGGSVINGHIEITDGVTITGMGMVMRAIDEKGMYSSGIPLQTNKEWRKTAARV
HKIDEMNKRLKAVEKKLVD

Sequences:

>Translated_339_residues
MSRITLAELAEKLDATVHGDGTVVVHSIASMSKAKEGDITFLSDAKYRKHLVECQASAVIVKEADVEFCQSNVLIMRDPY
LGFALAAQALDTTPAPASDIAPSAYIADDAIIGEGVAIGHNAVIESKAVIADGAMIGAGCFIGKEAKIGKNTKLWANVSV
YHRVEIGEACLVQSGTVIGSDGFGYANDRGTWVKIPQLGSVIIGDNVEIGANTTIDRGAIDDTVIESNVIIDNQIQIAHN
VQIGSGSAMAGGTIVAGSTKIGKHCIIGGGSVINGHIEITDGVTITGMGMVMRAIDEKGMYSSGIPLQTNKEWRKTAARV
HKIDEMNKRLKAVEKKLVD
>Mature_338_residues
SRITLAELAEKLDATVHGDGTVVVHSIASMSKAKEGDITFLSDAKYRKHLVECQASAVIVKEADVEFCQSNVLIMRDPYL
GFALAAQALDTTPAPASDIAPSAYIADDAIIGEGVAIGHNAVIESKAVIADGAMIGAGCFIGKEAKIGKNTKLWANVSVY
HRVEIGEACLVQSGTVIGSDGFGYANDRGTWVKIPQLGSVIIGDNVEIGANTTIDRGAIDDTVIESNVIIDNQIQIAHNV
QIGSGSAMAGGTIVAGSTKIGKHCIIGGGSVINGHIEITDGVTITGMGMVMRAIDEKGMYSSGIPLQTNKEWRKTAARVH
KIDEMNKRLKAVEKKLVD

Specific function: Lipid A biosynthesis; third step. [C]

COG id: COG1044

COG function: function code M; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family. LpxD subfamily

Homologues:

Organism=Escherichia coli, GI1786376, Length=337, Percent_Identity=62.6112759643917, Blast_Score=444, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPXD_VIBF1 (Q5E3E9)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205335.1
- ProteinModelPortal:   Q5E3E9
- STRING:   Q5E3E9
- GeneID:   3279215
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1952
- NMPDR:   fig|312309.3.peg.1952
- eggNOG:   COG1044
- HOGENOM:   HBG469615
- OMA:   CFVGKNT
- PhylomeDB:   Q5E3E9
- ProtClustDB:   PRK00892
- BioCyc:   VFIS312309:VF1952-MONOMER
- HAMAP:   MF_00523
- InterPro:   IPR001451
- InterPro:   IPR011004
- InterPro:   IPR007691
- InterPro:   IPR020573
- TIGRFAMs:   TIGR01853

Pfam domain/function: PF00132 Hexapep; PF04613 LpxD; SSF51161 Trimer_LpxA_like

EC number: 2.3.1.- [C]

Molecular weight: Translated: 35641; Mature: 35510

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRITLAELAEKLDATVHGDGTVVVHSIASMSKAKEGDITFLSDAKYRKHLVECQASAVI
CCCCCHHHHHHHHCCEECCCCEEEEEEHHHHHCCCCCCEEEEECCHHHHHHHHHCCCEEE
VKEADVEFCQSNVLIMRDPYLGFALAAQALDTTPAPASDIAPSAYIADDAIIGEGVAIGH
EEECCHHHHCCCEEEEECCCCCHHHHHHHHCCCCCCHHHCCCCCEEECCCEECCCEEECC
NAVIESKAVIADGAMIGAGCFIGKEAKIGKNTKLWANVSVYHRVEIGEACLVQSGTVIGS
CCEEECCEEEECCCEEECEEEECCCCCCCCCCEEEEEEEEEEEEECCCEEEEECCCEECC
DGFGYANDRGTWVKIPQLGSVIIGDNVEIGANTTIDRGAIDDTVIESNVIIDNQIQIAHN
CCCCCCCCCCCEEECCCCCCEEECCCEEECCCCCCCCCCCCCCEEECCEEECCEEEEEEE
VQIGSGSAMAGGTIVAGSTKIGKHCIIGGGSVINGHIEITDGVTITGMGMVMRAIDEKGM
EEECCCCCCCCCEEEECCCCCCCEEEEECCEEEEEEEEEECCEEEEHHHHHHHHHHHCCC
YSSGIPLQTNKEWRKTAARVHKIDEMNKRLKAVEKKLVD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRITLAELAEKLDATVHGDGTVVVHSIASMSKAKEGDITFLSDAKYRKHLVECQASAVI
CCCCHHHHHHHHCCEECCCCEEEEEEHHHHHCCCCCCEEEEECCHHHHHHHHHCCCEEE
VKEADVEFCQSNVLIMRDPYLGFALAAQALDTTPAPASDIAPSAYIADDAIIGEGVAIGH
EEECCHHHHCCCEEEEECCCCCHHHHHHHHCCCCCCHHHCCCCCEEECCCEECCCEEECC
NAVIESKAVIADGAMIGAGCFIGKEAKIGKNTKLWANVSVYHRVEIGEACLVQSGTVIGS
CCEEECCEEEECCCEEECEEEECCCCCCCCCCEEEEEEEEEEEEECCCEEEEECCCEECC
DGFGYANDRGTWVKIPQLGSVIIGDNVEIGANTTIDRGAIDDTVIESNVIIDNQIQIAHN
CCCCCCCCCCCEEECCCCCCEEECCCEEECCCCCCCCCCCCCCEEECCEEECCEEEEEEE
VQIGSGSAMAGGTIVAGSTKIGKHCIIGGGSVINGHIEITDGVTITGMGMVMRAIDEKGM
EEECCCCCCCCCEEEECCCCCCCEEEEECCEEEEEEEEEECCEEEEHHHHHHHHHHHCCC
YSSGIPLQTNKEWRKTAARVHKIDEMNKRLKAVEKKLVD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine; (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] [C]

Specific reaction: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine + (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] = UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine + [Acyl-Carrier Protein]. [C]

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA