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The map label for this gene is napF [H]

Identifier: 59712514

GI number: 59712514

Start: 2139452

End: 2139976

Strand: Reverse

Name: napF [H]

Synonym: VF_1907

Alternate gene names: 59712514

Gene position: 2139976-2139452 (Counterclockwise)

Preceding gene: 59712518

Following gene: 59712513

Centisome position: 73.86

GC content: 38.1

Gene sequence:

>525_bases
ATGAGTCAAGATAATGCAATTGACCATTCTAAGCGAGGATTTTTTCGCCGTTTATCTTCTCAAAAAAAAATAAATCCGTT
AACACAACAGCGATTGCCTTGGGTAGAAAATGAATCAGTTTTTACGTCAAAGTGTACTCGATGTGAAAAGTGCATTAATG
CATGCGAAGAAAATATTATTGTTAAAGGGGATGGTGGGTTTCCTATCGTCGATTTTACAAAAGGTGAATGTACTTTTTGT
GAAGGCTGTGCAAATAGCTGCCCTGAAGCTTTATTTGATTTAACGGCTGAGCCTGTTTTCTCTCATAAAATATCTATAAA
TGAAAACTGTTTAGCGAAAAAATCTGTGGAATGTCGAAGTTGTAGCGATATGTGTGAAACACAAGCCATTCGCTTTCAAC
TTCAGTTGGGCAGCGTAGCTCAACCTAAAATTAATTTTGATGCCTGTAATGGATGTGGTGGCTGTGTTGCTGTTTGCCCT
ACATCTGCTATCAGCATGACACTGGAAGATAGCACATCATGTTAG

Upstream 100 bases:

>100_bases
ATTTACCTATGTTTAACCATTATGGGGTATATCAATAAATATGACAAAATTTAAAATTCAACATATAAAAATGTTAATGA
ATAACTGAGGTTATGAATAG

Downstream 100 bases:

>100_bases
AGAATTTGCCTGAAAATGAAGTTCATATTTCAAGCTTAGTGGTTCATGTAAGACCTGAGTCACTGGAAATAACAAAAGAA
AAGATATTAGCACTGCCGAA

Product: ferredoxin-type protein, role in electron transfer to periplasmic nitrate reductase (NapA)

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MSQDNAIDHSKRGFFRRLSSQKKINPLTQQRLPWVENESVFTSKCTRCEKCINACEENIIVKGDGGFPIVDFTKGECTFC
EGCANSCPEALFDLTAEPVFSHKISINENCLAKKSVECRSCSDMCETQAIRFQLQLGSVAQPKINFDACNGCGGCVAVCP
TSAISMTLEDSTSC

Sequences:

>Translated_174_residues
MSQDNAIDHSKRGFFRRLSSQKKINPLTQQRLPWVENESVFTSKCTRCEKCINACEENIIVKGDGGFPIVDFTKGECTFC
EGCANSCPEALFDLTAEPVFSHKISINENCLAKKSVECRSCSDMCETQAIRFQLQLGSVAQPKINFDACNGCGGCVAVCP
TSAISMTLEDSTSC
>Mature_173_residues
SQDNAIDHSKRGFFRRLSSQKKINPLTQQRLPWVENESVFTSKCTRCEKCINACEENIIVKGDGGFPIVDFTKGECTFCE
GCANSCPEALFDLTAEPVFSHKISINENCLAKKSVECRSCSDMCETQAIRFQLQLGSVAQPKINFDACNGCGGCVAVCPT
SAISMTLEDSTSC

Specific function: Involved in electron transfer [H]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788536, Length=161, Percent_Identity=45.9627329192547, Blast_Score=149, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004496 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 19045; Mature: 18914

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00198 4FE4S_FERREDOXIN ; PS00242 INTEGRIN_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
11.5 %Cys+Met (Translated Protein)
9.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
11.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDNAIDHSKRGFFRRLSSQKKINPLTQQRLPWVENESVFTSKCTRCEKCINACEENII
CCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEE
VKGDGGFPIVDFTKGECTFCEGCANSCPEALFDLTAEPVFSHKISINENCLAKKSVECRS
EECCCCCEEEECCCCCCEEHHHHHHCCHHHHHHCCCCCHHHEEECCCCCHHHHCCCCHHH
CSDMCETQAIRFQLQLGSVAQPKINFDACNGCGGCVAVCPTSAISMTLEDSTSC
HHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCC
>Mature Secondary Structure 
SQDNAIDHSKRGFFRRLSSQKKINPLTQQRLPWVENESVFTSKCTRCEKCINACEENII
CCCCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEE
VKGDGGFPIVDFTKGECTFCEGCANSCPEALFDLTAEPVFSHKISINENCLAKKSVECRS
EECCCCCEEEECCCCCCEEHHHHHHCCHHHHHHCCCCCHHHEEECCCCCHHHHCCCCHHH
CSDMCETQAIRFQLQLGSVAQPKINFDACNGCGGCVAVCPTSAISMTLEDSTSC
HHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]