Click here to switch to the map view.

The map label for this gene is cysK [H]

Identifier: 59712500

GI number: 59712500

Start: 2128845

End: 2129813

Strand: Direct

Name: cysK [H]

Synonym: VF_1893

Alternate gene names: 59712500

Gene position: 2128845-2129813 (Clockwise)

Preceding gene: 59712499

Following gene: 59712501

Centisome position: 73.47

GC content: 42.21

Gene sequence:

>969_bases
ATGACAAAGATTTACGAAGATAATTCTCAAACTATTGGTAATACACCACTTGTTCGCCTAAACCGTGTAAGCAAAGGTAA
CGTTCTTGCAAAAGTAGAAGCTCGTAACCCTAGTTTTAGTGTTAAATGTCGTATTGGCGCCAACATGATTTGGGAAGCGG
AAAAAGCTGGTACGCTAAAAGAAGGGATTGAACTAGTAGAGCCTACATCAGGTAATACAGGTATTGCGCTTGCTTTTGTT
GCTGCAGCACGTGGTTATAAACTAACTCTAACGATGCCAGCCTCAATGAGCCTTGAGCGTCGTAAACTACTTAAAGCATT
AGGCGCAAACCTTGTTTTGACTGAAGCGCCTAAAGGTATGGGTGGAGCTATTGCTAAAGCTGAAGAAATTGTTGCAAGCG
ACCCAAGTAAGTACCTACTTCTTCAACAATTTAACAACCCAGCAAACCCTGCTATTCATGAAAAAACAACAGGTCCAGAA
ATTTGGGAAGCAACTGAAGGCAATGTTGATGTGTTCGTAGCCGGTGTAGGTACAGGTGGCACATTAACAGGTACAAGCCG
TTACCTAAAACACACCCAGAATAAAGCCGTTCTTTCTGTTGCTGTTGAACCAGCAGAGTCTCCAGTTATTGCTCAAGCGT
TAGCTGGTGAGGAGATCAAACCTGCTCCACATAAGATCCAAGGTATTGGTGCTGGTTTCATCCCTGGTAACTTAGATCTA
ACACTAATTGACCGTGTTGAAGCAGTAACATCTGAAGATGCAATTGAAATGGCTCGCCGATTAATGGAAGAAGAAGGGAT
TCTTGCTGGTATTTCTTCTGGTGCAGCTGTAGTCGCAGCTAATCGAATTGCAGAACTACCTGAATTTGCTAATAAACAAA
TTGTTGTAGTGTTACCAAGCTCTGGTGAGCGTTACTTAAGCACAGCATTGTTTGCGGGTTTGTTTACTGAACAAGAAAAT
GAGCAATAA

Upstream 100 bases:

>100_bases
CAATTTAAAAACTCTTCGTATTCTAGTTGTGTTGAGTTACTCAAATTGAGTTAAAAATAATTTATATAATCACGTTGAGC
GTGAAATGAAGGAACAGATC

Downstream 100 bases:

>100_bases
ATTTGTAACATCTATGTGTTCAAATCAATTTTTTATCCAAAAAAGCCCCTTCTGGGGTTTTTTTGTTGATTTCATTTCCC
TCCTTAGTAATAATCAAGCC

Product: cysteine synthase A, O-acetylserine sulfhydrolase A subunit

Products: NA

Alternate protein names: CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; Sulfate starvation-induced protein 5; SSI5 [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTKIYEDNSQTIGNTPLVRLNRVSKGNVLAKVEARNPSFSVKCRIGANMIWEAEKAGTLKEGIELVEPTSGNTGIALAFV
AAARGYKLTLTMPASMSLERRKLLKALGANLVLTEAPKGMGGAIAKAEEIVASDPSKYLLLQQFNNPANPAIHEKTTGPE
IWEATEGNVDVFVAGVGTGGTLTGTSRYLKHTQNKAVLSVAVEPAESPVIAQALAGEEIKPAPHKIQGIGAGFIPGNLDL
TLIDRVEAVTSEDAIEMARRLMEEEGILAGISSGAAVVAANRIAELPEFANKQIVVVLPSSGERYLSTALFAGLFTEQEN
EQ

Sequences:

>Translated_322_residues
MTKIYEDNSQTIGNTPLVRLNRVSKGNVLAKVEARNPSFSVKCRIGANMIWEAEKAGTLKEGIELVEPTSGNTGIALAFV
AAARGYKLTLTMPASMSLERRKLLKALGANLVLTEAPKGMGGAIAKAEEIVASDPSKYLLLQQFNNPANPAIHEKTTGPE
IWEATEGNVDVFVAGVGTGGTLTGTSRYLKHTQNKAVLSVAVEPAESPVIAQALAGEEIKPAPHKIQGIGAGFIPGNLDL
TLIDRVEAVTSEDAIEMARRLMEEEGILAGISSGAAVVAANRIAELPEFANKQIVVVLPSSGERYLSTALFAGLFTEQEN
EQ
>Mature_321_residues
TKIYEDNSQTIGNTPLVRLNRVSKGNVLAKVEARNPSFSVKCRIGANMIWEAEKAGTLKEGIELVEPTSGNTGIALAFVA
AARGYKLTLTMPASMSLERRKLLKALGANLVLTEAPKGMGGAIAKAEEIVASDPSKYLLLQQFNNPANPAIHEKTTGPEI
WEATEGNVDVFVAGVGTGGTLTGTSRYLKHTQNKAVLSVAVEPAESPVIAQALAGEEIKPAPHKIQGIGAGFIPGNLDLT
LIDRVEAVTSEDAIEMARRLMEEEGILAGISSGAAVVAANRIAELPEFANKQIVVVLPSSGERYLSTALFAGLFTEQENE
Q

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=320, Percent_Identity=38.75, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI295821200, Length=320, Percent_Identity=38.75, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI4557415, Length=320, Percent_Identity=38.75, Blast_Score=197, Evalue=1e-50,
Organism=Escherichia coli, GI1788754, Length=323, Percent_Identity=78.6377708978328, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI2367138, Length=308, Percent_Identity=44.4805194805195, Blast_Score=222, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI17535051, Length=315, Percent_Identity=52.6984126984127, Blast_Score=295, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17562970, Length=316, Percent_Identity=50.9493670886076, Blast_Score=291, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI32566674, Length=307, Percent_Identity=51.7915309446254, Blast_Score=284, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI115535073, Length=315, Percent_Identity=53.6507936507937, Blast_Score=281, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI17561720, Length=316, Percent_Identity=49.6835443037975, Blast_Score=261, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI32566672, Length=226, Percent_Identity=49.1150442477876, Blast_Score=209, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17534315, Length=321, Percent_Identity=40.1869158878505, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI25147552, Length=327, Percent_Identity=36.085626911315, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17561716, Length=131, Percent_Identity=49.618320610687, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=36.8253968253968, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6321449, Length=324, Percent_Identity=34.5679012345679, Blast_Score=132, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24643623, Length=309, Percent_Identity=35.9223300970874, Blast_Score=154, Evalue=6e-38,
Organism=Drosophila melanogaster, GI20129101, Length=309, Percent_Identity=35.9223300970874, Blast_Score=154, Evalue=6e-38,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34088; Mature: 33957

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIYEDNSQTIGNTPLVRLNRVSKGNVLAKVEARNPSFSVKCRIGANMIWEAEKAGTLK
CCEEECCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCEEEEEEECCCEEEECHHCCCHH
EGIELVEPTSGNTGIALAFVAAARGYKLTLTMPASMSLERRKLLKALGANLVLTEAPKGM
HCCEEECCCCCCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCC
GGAIAKAEEIVASDPSKYLLLQQFNNPANPAIHEKTTGPEIWEATEGNVDVFVAGVGTGG
CCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCEECCCCCEEEEEEEECCCC
TLTGTSRYLKHTQNKAVLSVAVEPAESPVIAQALAGEEIKPAPHKIQGIGAGFIPGNLDL
CCCCCHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCE
TLIDRVEAVTSEDAIEMARRLMEEEGILAGISSGAAVVAANRIAELPEFANKQIVVVLPS
EEEHHHHHHCCHHHHHHHHHHHHHCCCEEECCCCCEEEEHHHHHHHHHHCCCEEEEEECC
SGERYLSTALFAGLFTEQENEQ
CCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TKIYEDNSQTIGNTPLVRLNRVSKGNVLAKVEARNPSFSVKCRIGANMIWEAEKAGTLK
CEEECCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCEEEEEEECCCEEEECHHCCCHH
EGIELVEPTSGNTGIALAFVAAARGYKLTLTMPASMSLERRKLLKALGANLVLTEAPKGM
HCCEEECCCCCCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCC
GGAIAKAEEIVASDPSKYLLLQQFNNPANPAIHEKTTGPEIWEATEGNVDVFVAGVGTGG
CCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCEECCCCCEEEEEEEECCCC
TLTGTSRYLKHTQNKAVLSVAVEPAESPVIAQALAGEEIKPAPHKIQGIGAGFIPGNLDL
CCCCCHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCE
TLIDRVEAVTSEDAIEMARRLMEEEGILAGISSGAAVVAANRIAELPEFANKQIVVVLPS
EEEHHHHHHCCHHHHHHHHHHHHHCCCEEECCCCCEEEEHHHHHHHHHHCCCEEEEEECC
SGERYLSTALFAGLFTEQENEQ
CCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]