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The map label for this gene is flrA [H]

Identifier: 59712463

GI number: 59712463

Start: 2088202

End: 2089683

Strand: Reverse

Name: flrA [H]

Synonym: VF_1856

Alternate gene names: 59712463

Gene position: 2089683-2088202 (Counterclockwise)

Preceding gene: 59712465

Following gene: 59712462

Centisome position: 72.12

GC content: 40.89

Gene sequence:

>1482_bases
ATGCAAGGTTTAGCAAAGACTCTTATTATTGAAGATGATGCTCAGAGCCGTCATAATCTTAAGGTGATCCTTGAGTTTAT
CGGTGAACATTGTCATGCCATTTCAGCTTCAGAATTGTCTACTTTTGAGTGGAGTGAGCCATGGAGTGCTTGTTTTTTAG
GTCAAGTTTCTGACCAAGCGCTGAAAGATATAATCAAAAGTTCATTAGTTGTACATAATCATATTCCAGTCATTATGCTT
GCGGGGGCTATGCATGATGTTGAGGAATTGACAAACTATGTAGGTGAACTGAATCAACCAGTTAATTATCCTCAATTAAC
CGATGCGTTACGTCATTGCCAAGAATTTATGGGTCGTCGTGGGTTAGAAGTACCCCACCTAGGCCGTAAAAATACGTTAT
TCCGTAGTATGGTTGGACAAAGTACAGCTATTAGTACTGTTCGCCATTTAATTGAACAAGTTTCAGGTACAGAAGCAAGT
GTATTGGTTTTGGGTGAATCGGGTACGGGTAAAGAAGTAGTTGCACGTAACTTGCACTATCATTCGCCACGCCGTAAAGG
ACCATTTGTTCCGGTTAACTGTGGTGCAATCCCACCAGATCTTCTAGAAAGTGAACTGTTTGGCCATGAGAAAGGGGCGT
TTACTGGTGCAATTACTGCACGTAAAGGTCGCTTTGAACTTGCGCAAGGCGGTACGTTATTTTTAGATGAAATTGGTGAT
ATGCCAATGCCGATGCAGGTGAAATTACTTCGTGTTCTTCAAGAGCGTTGTTTTGAGCGTGTAGGCGGCAATCAAAGTAT
TCGTGCAGATGTTCGTATTGTTGCTGCGACGCACCGTAATTTAGATGAGATGATAGAGAAAGGCACATTCCGTGAAGATC
TGTATTATCGTTTAAATGTTTTCCCAATTGAAATGCCAGCATTACGTGAACGTAAGCAAGATATTCCTTTGTTGCTGCAA
GAGTTATTAGCAAGAATGGAAGCTGAAGGCAGTACACGTGTACGACTAACTCCTCGTGCCGTTAACTCGTTAATGGAACA
CCATTGGCCTGGTAACGTACGTGAATTAGCAAACTTAATTGAGCGTTTGATGATCTTATTCCCTGGGGAATCGGTGGACG
TAAATCACTTACCAACGAAGTATCGTTATAGTGATATTCCAGAGTTCCAACCTGAGAATTACAATCAGCAATCAATTGAA
GAGCAAGAACAAGCGGCATTAGCTGACATGTTTGCTAACTTTAGTGATGGTGGTATTCCTCAACAAGGAATGCAAAACGC
AGGACCAACATCGAATGATTTACCAGAAGATGGTTTAAATCTGAAAGAGATGTTAGCTGAAGTTGAAGTTAATATGATAT
GCCAAGCACTTGAAGCGCAAAATGGTGTTGTTGCACGAGCAGCTGACATGTTAGGTATGCGTCGTACTACTCTTGTTGAG
AAAATGCGTAAATATAATTTAAATAAAGATGATATGCGTTAA

Upstream 100 bases:

>100_bases
AGTTGCCTTATTTTTCTTATGCTATAATATGTGGGCAATATTTCGTTGCTGTTCAATGAATAGACCACATAAATCAAAGA
ATTAAGGCATATCCACATCT

Downstream 100 bases:

>100_bases
TACTCTTTAGTCAAAAAGAGATAACGTTTCACTAATTTAAATGACAAAAATTTGACCGTCAAGCTTACATGTGCAGTGTA
AGTTATTGATAATAAATACC

Product: sigma-54-dependent transcriptional activator FlrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MQGLAKTLIIEDDAQSRHNLKVILEFIGEHCHAISASELSTFEWSEPWSACFLGQVSDQALKDIIKSSLVVHNHIPVIML
AGAMHDVEELTNYVGELNQPVNYPQLTDALRHCQEFMGRRGLEVPHLGRKNTLFRSMVGQSTAISTVRHLIEQVSGTEAS
VLVLGESGTGKEVVARNLHYHSPRRKGPFVPVNCGAIPPDLLESELFGHEKGAFTGAITARKGRFELAQGGTLFLDEIGD
MPMPMQVKLLRVLQERCFERVGGNQSIRADVRIVAATHRNLDEMIEKGTFREDLYYRLNVFPIEMPALRERKQDIPLLLQ
ELLARMEAEGSTRVRLTPRAVNSLMEHHWPGNVRELANLIERLMILFPGESVDVNHLPTKYRYSDIPEFQPENYNQQSIE
EQEQAALADMFANFSDGGIPQQGMQNAGPTSNDLPEDGLNLKEMLAEVEVNMICQALEAQNGVVARAADMLGMRRTTLVE
KMRKYNLNKDDMR

Sequences:

>Translated_493_residues
MQGLAKTLIIEDDAQSRHNLKVILEFIGEHCHAISASELSTFEWSEPWSACFLGQVSDQALKDIIKSSLVVHNHIPVIML
AGAMHDVEELTNYVGELNQPVNYPQLTDALRHCQEFMGRRGLEVPHLGRKNTLFRSMVGQSTAISTVRHLIEQVSGTEAS
VLVLGESGTGKEVVARNLHYHSPRRKGPFVPVNCGAIPPDLLESELFGHEKGAFTGAITARKGRFELAQGGTLFLDEIGD
MPMPMQVKLLRVLQERCFERVGGNQSIRADVRIVAATHRNLDEMIEKGTFREDLYYRLNVFPIEMPALRERKQDIPLLLQ
ELLARMEAEGSTRVRLTPRAVNSLMEHHWPGNVRELANLIERLMILFPGESVDVNHLPTKYRYSDIPEFQPENYNQQSIE
EQEQAALADMFANFSDGGIPQQGMQNAGPTSNDLPEDGLNLKEMLAEVEVNMICQALEAQNGVVARAADMLGMRRTTLVE
KMRKYNLNKDDMR
>Mature_493_residues
MQGLAKTLIIEDDAQSRHNLKVILEFIGEHCHAISASELSTFEWSEPWSACFLGQVSDQALKDIIKSSLVVHNHIPVIML
AGAMHDVEELTNYVGELNQPVNYPQLTDALRHCQEFMGRRGLEVPHLGRKNTLFRSMVGQSTAISTVRHLIEQVSGTEAS
VLVLGESGTGKEVVARNLHYHSPRRKGPFVPVNCGAIPPDLLESELFGHEKGAFTGAITARKGRFELAQGGTLFLDEIGD
MPMPMQVKLLRVLQERCFERVGGNQSIRADVRIVAATHRNLDEMIEKGTFREDLYYRLNVFPIEMPALRERKQDIPLLLQ
ELLARMEAEGSTRVRLTPRAVNSLMEHHWPGNVRELANLIERLMILFPGESVDVNHLPTKYRYSDIPEFQPENYNQQSIE
EQEQAALADMFANFSDGGIPQQGMQNAGPTSNDLPEDGLNLKEMLAEVEVNMICQALEAQNGVVARAADMLGMRRTTLVE
KMRKYNLNKDDMR

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=341, Percent_Identity=44.2815249266862, Blast_Score=281, Evalue=9e-77,
Organism=Escherichia coli, GI87082117, Length=356, Percent_Identity=43.8202247191011, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1790299, Length=499, Percent_Identity=35.8717434869739, Blast_Score=272, Evalue=4e-74,
Organism=Escherichia coli, GI1790437, Length=349, Percent_Identity=42.9799426934097, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI1789087, Length=368, Percent_Identity=40.4891304347826, Blast_Score=255, Evalue=6e-69,
Organism=Escherichia coli, GI1789233, Length=354, Percent_Identity=41.2429378531073, Blast_Score=248, Evalue=5e-67,
Organism=Escherichia coli, GI1788905, Length=221, Percent_Identity=54.7511312217195, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI87082152, Length=231, Percent_Identity=48.4848484848485, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI1786524, Length=276, Percent_Identity=45.2898550724638, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI87081872, Length=229, Percent_Identity=45.4148471615721, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1787583, Length=239, Percent_Identity=43.0962343096234, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI87081858, Length=374, Percent_Identity=28.3422459893048, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1789828, Length=251, Percent_Identity=34.6613545816733, Blast_Score=143, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55399; Mature: 55399

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGLAKTLIIEDDAQSRHNLKVILEFIGEHCHAISASELSTFEWSEPWSACFLGQVSDQA
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHH
LKDIIKSSLVVHNHIPVIMLAGAMHDVEELTNYVGELNQPVNYPQLTDALRHCQEFMGRR
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
GLEVPHLGRKNTLFRSMVGQSTAISTVRHLIEQVSGTEASVLVLGESGTGKEVVARNLHY
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHC
HSPRRKGPFVPVNCGAIPPDLLESELFGHEKGAFTGAITARKGRFELAQGGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEECCCCEEEHHHHCC
MPMPMQVKLLRVLQERCFERVGGNQSIRADVRIVAATHRNLDEMIEKGTFREDLYYRLNV
CCCCHHHHHHHHHHHHHHHHHCCCCCEECCEEEEEHHHCCHHHHHHCCCCHHHHEEEEEE
FPIEMPALRERKQDIPLLLQELLARMEAEGSTRVRLTPRAVNSLMEHHWPGNVRELANLI
EEECCHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHCCCCCHHHHHHHH
ERLMILFPGESVDVNHLPTKYRYSDIPEFQPENYNQQSIEEQEQAALADMFANFSDGGIP
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
QQGMQNAGPTSNDLPEDGLNLKEMLAEVEVNMICQALEAQNGVVARAADMLGMRRTTLVE
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KMRKYNLNKDDMR
HHHHCCCCCCCCC
>Mature Secondary Structure
MQGLAKTLIIEDDAQSRHNLKVILEFIGEHCHAISASELSTFEWSEPWSACFLGQVSDQA
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHH
LKDIIKSSLVVHNHIPVIMLAGAMHDVEELTNYVGELNQPVNYPQLTDALRHCQEFMGRR
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
GLEVPHLGRKNTLFRSMVGQSTAISTVRHLIEQVSGTEASVLVLGESGTGKEVVARNLHY
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHC
HSPRRKGPFVPVNCGAIPPDLLESELFGHEKGAFTGAITARKGRFELAQGGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEECCCCEEEHHHHCC
MPMPMQVKLLRVLQERCFERVGGNQSIRADVRIVAATHRNLDEMIEKGTFREDLYYRLNV
CCCCHHHHHHHHHHHHHHHHHCCCCCEECCEEEEEHHHCCHHHHHHCCCCHHHHEEEEEE
FPIEMPALRERKQDIPLLLQELLARMEAEGSTRVRLTPRAVNSLMEHHWPGNVRELANLI
EEECCHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHCCCCCHHHHHHHH
ERLMILFPGESVDVNHLPTKYRYSDIPEFQPENYNQQSIEEQEQAALADMFANFSDGGIP
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
QQGMQNAGPTSNDLPEDGLNLKEMLAEVEVNMICQALEAQNGVVARAADMLGMRRTTLVE
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KMRKYNLNKDDMR
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2881918; 11481430; 8478331 [H]