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The map label for this gene is fliF [H]

Identifier: 59712459

GI number: 59712459

Start: 2083382

End: 2085115

Strand: Reverse

Name: fliF [H]

Synonym: VF_1852

Alternate gene names: 59712459

Gene position: 2085115-2083382 (Counterclockwise)

Preceding gene: 59712460

Following gene: 59712458

Centisome position: 71.96

GC content: 41.29

Gene sequence:

>1734_bases
GTGTCGGAAGAGAACAAAAACACGAACTTAGCCGTATCGGATGATATGCCAACAGGCGGTGCTATGGCACTGGATAGCTC
TGTTGACGTACAAAATCCAGATGTTGATGAGCAAAGTTCTTCAAAATTAGATTCAGCTTTGGGTAATCTTGACTTATTAC
GTCAGGTTATCCTCGTATTATCTGTTTCTATTTGTGTGGCGTTAATTGTCATGCTTTTCTTTTGGGTTCAAGAACCAGAG
ATGCGTCCGCTTGGAGTTTATGAAACAGATGAACTAATCCAAGTGCTTGATCATCTTGATGCTCAAAAAGCAGAATACAA
ACTAGATGGTAATACCATTTTAGTTCCCGCCTCTGAATTCAGTAAAATGAATCTTGATTTGTCTCGCGCAGGCTTAAATA
AAGCCGTTGGTGAGGGAGATGATATTTTATTGCAAGATATGGGCTTTGGGGTTTCACAGCGTTTAGAGCAAGAGCGTCTA
AAACTAAGTCGTGAACGCCAATTAGCAAGTACATTTGAGCAAATGAGACCAATTCGTAAAGCTCAAGTATTACTCGCAAT
GCCAAAACAAAGCGTATTTGTTCGTCATAATCAAGAAGCGTCAGCAACGGTATTCTTGACCATGCGAATGGGTGCAATGC
TAAAACAAGAAGAAGTAGATTCGATTGTTGATATGGTTGCAAGTGCGGTTCCAGGAATGAAACCAACACGTGTTACCGTA
ACGGATCAGCATGGTCGTCTTCTTAGCTCTGGTACGCAAGATCCGATGGCAATGGCTCATCGAAAAGAGCAAGAGTTAGA
GCGTAAACAAGAGCAATCTTTACGAGATAAAATTGACGCAATCCTTATTCCTATTTTGGGTTTAGGTAACTATACAGCTC
AAGTGGATGTGTCTATGGATTTCAGTGCTGTTGAGCAAACTCGTAAGCAATTTGATCCTCAAACGCCATCTACTCGAAGT
GAATACACGTTAGAAGATTATAACAACGGTAATGTGATTGCAGGTGTTCCTGGTGCGCTAAGTAACCAACCGCCGGTAGA
TTCCGCTATCCCTCAAGATATCGCAGAATTAAAAGATGGTTCGGTATTAGGTCAAGGTTCAGTTCATAAAGAAGCAACAC
GAAACTTTGAACTTGATACAACAATCAGTCACGAACGTAAACAAACAGGAACCGTAGCTCGACAAACGGTATCTGTTGCC
GTTAAAGAGCGTCAAAGCGTTAACCCTGAAACAGGTGAAGTTACCTATACACCAATTTCAGCAGCAGAATTAGATAAGAT
CCGTCAAATCATTATTGGTAGTGTTGGTCTAAATGAACAACGTGGTGATTTACTGAACGTACTGAGCGTGAAGTTTGCTA
TTGAAGAGCAACCAATTGCGATTGATGTGCCAATTTGGGAAAACCCTAACTTTAACGATTGGGTTAAATGGCTTGCTGGT
GCCTTGGTTATTATCGCCGTTATTCTTGTTCTTGTTCGTCCAGCAATGAATAAATTACTGTTCCCTAATCAAGCAGAAGA
TGAAGCATTGGCAAATGCAGAACGTAATGCTGAAGGTGAGATGGGCTTAATTGGCAGTGATTTAGATGGAGATGGATTCG
AGTTTGGTTCGGGCATCGATCTTCCTAACTTGCATAAAGACGAAGATTTATTGAAAGCGGTTCGTGCGCTAGTAGCAAAT
GAACCGGAATTAGCAGCACAAGTAGTTCGAAACTGGGTATCTGAAAATGGCTAA

Upstream 100 bases:

>100_bases
CAAGCGTTGCATTTGATGCCACAATTCAAGTGCGCAATAAGCTAGTTGATGCTTATAAAGCACTGATGAATATGCCAGTC
TAATTTCTAGGAATTAAAGC

Downstream 100 bases:

>100_bases
TGAAATTGTAGAAGCAGGAAATAAAGAAGAAGAAACACCAGAATTTGATCTTTCTTCATTATCAGGCTCAGAGCGTGCAG
CGATTTTATTGCTAAGTTTA

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MSEENKNTNLAVSDDMPTGGAMALDSSVDVQNPDVDEQSSSKLDSALGNLDLLRQVILVLSVSICVALIVMLFFWVQEPE
MRPLGVYETDELIQVLDHLDAQKAEYKLDGNTILVPASEFSKMNLDLSRAGLNKAVGEGDDILLQDMGFGVSQRLEQERL
KLSRERQLASTFEQMRPIRKAQVLLAMPKQSVFVRHNQEASATVFLTMRMGAMLKQEEVDSIVDMVASAVPGMKPTRVTV
TDQHGRLLSSGTQDPMAMAHRKEQELERKQEQSLRDKIDAILIPILGLGNYTAQVDVSMDFSAVEQTRKQFDPQTPSTRS
EYTLEDYNNGNVIAGVPGALSNQPPVDSAIPQDIAELKDGSVLGQGSVHKEATRNFELDTTISHERKQTGTVARQTVSVA
VKERQSVNPETGEVTYTPISAAELDKIRQIIIGSVGLNEQRGDLLNVLSVKFAIEEQPIAIDVPIWENPNFNDWVKWLAG
ALVIIAVILVLVRPAMNKLLFPNQAEDEALANAERNAEGEMGLIGSDLDGDGFEFGSGIDLPNLHKDEDLLKAVRALVAN
EPELAAQVVRNWVSENG

Sequences:

>Translated_577_residues
MSEENKNTNLAVSDDMPTGGAMALDSSVDVQNPDVDEQSSSKLDSALGNLDLLRQVILVLSVSICVALIVMLFFWVQEPE
MRPLGVYETDELIQVLDHLDAQKAEYKLDGNTILVPASEFSKMNLDLSRAGLNKAVGEGDDILLQDMGFGVSQRLEQERL
KLSRERQLASTFEQMRPIRKAQVLLAMPKQSVFVRHNQEASATVFLTMRMGAMLKQEEVDSIVDMVASAVPGMKPTRVTV
TDQHGRLLSSGTQDPMAMAHRKEQELERKQEQSLRDKIDAILIPILGLGNYTAQVDVSMDFSAVEQTRKQFDPQTPSTRS
EYTLEDYNNGNVIAGVPGALSNQPPVDSAIPQDIAELKDGSVLGQGSVHKEATRNFELDTTISHERKQTGTVARQTVSVA
VKERQSVNPETGEVTYTPISAAELDKIRQIIIGSVGLNEQRGDLLNVLSVKFAIEEQPIAIDVPIWENPNFNDWVKWLAG
ALVIIAVILVLVRPAMNKLLFPNQAEDEALANAERNAEGEMGLIGSDLDGDGFEFGSGIDLPNLHKDEDLLKAVRALVAN
EPELAAQVVRNWVSENG
>Mature_576_residues
SEENKNTNLAVSDDMPTGGAMALDSSVDVQNPDVDEQSSSKLDSALGNLDLLRQVILVLSVSICVALIVMLFFWVQEPEM
RPLGVYETDELIQVLDHLDAQKAEYKLDGNTILVPASEFSKMNLDLSRAGLNKAVGEGDDILLQDMGFGVSQRLEQERLK
LSRERQLASTFEQMRPIRKAQVLLAMPKQSVFVRHNQEASATVFLTMRMGAMLKQEEVDSIVDMVASAVPGMKPTRVTVT
DQHGRLLSSGTQDPMAMAHRKEQELERKQEQSLRDKIDAILIPILGLGNYTAQVDVSMDFSAVEQTRKQFDPQTPSTRSE
YTLEDYNNGNVIAGVPGALSNQPPVDSAIPQDIAELKDGSVLGQGSVHKEATRNFELDTTISHERKQTGTVARQTVSVAV
KERQSVNPETGEVTYTPISAAELDKIRQIIIGSVGLNEQRGDLLNVLSVKFAIEEQPIAIDVPIWENPNFNDWVKWLAGA
LVIIAVILVLVRPAMNKLLFPNQAEDEALANAERNAEGEMGLIGSDLDGDGFEFGSGIDLPNLHKDEDLLKAVRALVANE
PELAAQVVRNWVSENG

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=448, Percent_Identity=31.9196428571429, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 63388; Mature: 63256

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEENKNTNLAVSDDMPTGGAMALDSSVDVQNPDVDEQSSSKLDSALGNLDLLRQVILVL
CCCCCCCCCEEEECCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHH
SVSICVALIVMLFFWVQEPEMRPLGVYETDELIQVLDHLDAQKAEYKLDGNTILVPASEF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCEEEEECHHH
SKMNLDLSRAGLNKAVGEGDDILLQDMGFGVSQRLEQERLKLSRERQLASTFEQMRPIRK
HHCCCCHHHHCHHHHCCCCCCEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQVLLAMPKQSVFVRHNQEASATVFLTMRMGAMLKQEEVDSIVDMVASAVPGMKPTRVTV
HHHHHCCCCCCEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
TDQHGRLLSSGTQDPMAMAHRKEQELERKQEQSLRDKIDAILIPILGLGNYTAQVDVSMD
ECCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
FSAVEQTRKQFDPQTPSTRSEYTLEDYNNGNVIAGVPGALSNQPPVDSAIPQDIAELKDG
HHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCHHHHHHHCCC
SVLGQGSVHKEATRNFELDTTISHERKQTGTVARQTVSVAVKERQSVNPETGEVTYTPIS
CEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCC
AAELDKIRQIIIGSVGLNEQRGDLLNVLSVKFAIEEQPIAIDVPIWENPNFNDWVKWLAG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCCEEEEECCCCCCCHHHHHHHHHH
ALVIIAVILVLVRPAMNKLLFPNQAEDEALANAERNAEGEMGLIGSDLDGDGFEFGSGID
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCC
LPNLHKDEDLLKAVRALVANEPELAAQVVRNWVSENG
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEENKNTNLAVSDDMPTGGAMALDSSVDVQNPDVDEQSSSKLDSALGNLDLLRQVILVL
CCCCCCCCEEEECCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHH
SVSICVALIVMLFFWVQEPEMRPLGVYETDELIQVLDHLDAQKAEYKLDGNTILVPASEF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCEEEEECHHH
SKMNLDLSRAGLNKAVGEGDDILLQDMGFGVSQRLEQERLKLSRERQLASTFEQMRPIRK
HHCCCCHHHHCHHHHCCCCCCEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQVLLAMPKQSVFVRHNQEASATVFLTMRMGAMLKQEEVDSIVDMVASAVPGMKPTRVTV
HHHHHCCCCCCEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
TDQHGRLLSSGTQDPMAMAHRKEQELERKQEQSLRDKIDAILIPILGLGNYTAQVDVSMD
ECCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
FSAVEQTRKQFDPQTPSTRSEYTLEDYNNGNVIAGVPGALSNQPPVDSAIPQDIAELKDG
HHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCHHHHHHHCCC
SVLGQGSVHKEATRNFELDTTISHERKQTGTVARQTVSVAVKERQSVNPETGEVTYTPIS
CEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCC
AAELDKIRQIIIGSVGLNEQRGDLLNVLSVKFAIEEQPIAIDVPIWENPNFNDWVKWLAG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCCEEEEECCCCCCCHHHHHHHHHH
ALVIIAVILVLVRPAMNKLLFPNQAEDEALANAERNAEGEMGLIGSDLDGDGFEFGSGID
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCC
LPNLHKDEDLLKAVRALVANEPELAAQVVRNWVSENG
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8675317; 10984043 [H]