Click here to switch to the map view.

The map label for this gene is vacJ [H]

Identifier: 59712422

GI number: 59712422

Start: 2050906

End: 2051688

Strand: Reverse

Name: vacJ [H]

Synonym: VF_1815

Alternate gene names: 59712422

Gene position: 2051688-2050906 (Counterclockwise)

Preceding gene: 59712423

Following gene: 59712418

Centisome position: 70.81

GC content: 39.21

Gene sequence:

>783_bases
GTGAAAAGAGTATGGATTTCTTGTTGTATTCTACTTTTTGTGAGTGCTTGCTCACAAACACCAGAACAAACAGAAGAAGA
GTTAGATACAACACAACAGATTGATACGGAATATGAAGAAACGCACGTAAGTGATCCTCTTGAAGGATTTAACCGTGTAA
TGTGGGGTCTAAATTATGATTATTTAGACCCATATCTAGTTCGCCCTGTGTCTATTGGTTATGTCGAATACACGCCAACC
CCAATTCGATATGGGATTGCAAACTTTTTAGCTAATTTAGATGAGCCATCAAGCATGATAAATAATTTACTCATGGGAAA
TGGCAATATGGCGATGGTTCACTTTAATCGTTTTTGGCTAAATACAACATTAGGCCTCGTTGGCTTAATTGATATTGCTA
GTGCTGCAGATATACAGAAACCAGACAGTAAATCGTTTGGTGATGCTTTAGGACATTATGGTGTAGGTAATGGCCCTTAC
TTTATGCTACCTGGCTACGGGCCTTGGACACTGCGAGAAGCTGGGGATTTTGTTGATGGTTTATATATGCCGCTGTCATA
TCTTAACTTCTGGCAAGGTTTAGGTAAGTGGGGATTAGAAGGCATGGAATCTCGTGTACAGCTGATTTCGCAAGAACCGA
TGCTAGAAGCGTCACCAGATCCTTACGTATTAACACGAGATGTTTATTTACAACGTCAAGACTTTAAAGCTGAGATTGAA
CCTGAAGTTGATTTAGATGCTGAAGATGATATCGATGACTATTTAGATGAAATAGACGAGTAA

Upstream 100 bases:

>100_bases
CATCTATAATAATAAAATACATATAGTTATACCAATCATACTAAGTGAACAATTATTTATATCGATTGGTACAGTTACTT
ATTGGCAAAGGTTTTTAGTA

Downstream 100 bases:

>100_bases
AAACAGTAATAGTCTTTCAGGTTTCACGGTCAGTATTCTCCTGAAACCTGAAACCTGAAACCTGAAACCTGAAACCTAGC
GATTATTCCGCTGGAAAAAT

Product: VacJ lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKRVWISCCILLFVSACSQTPEQTEEELDTTQQIDTEYEETHVSDPLEGFNRVMWGLNYDYLDPYLVRPVSIGYVEYTPT
PIRYGIANFLANLDEPSSMINNLLMGNGNMAMVHFNRFWLNTTLGLVGLIDIASAADIQKPDSKSFGDALGHYGVGNGPY
FMLPGYGPWTLREAGDFVDGLYMPLSYLNFWQGLGKWGLEGMESRVQLISQEPMLEASPDPYVLTRDVYLQRQDFKAEIE
PEVDLDAEDDIDDYLDEIDE

Sequences:

>Translated_260_residues
MKRVWISCCILLFVSACSQTPEQTEEELDTTQQIDTEYEETHVSDPLEGFNRVMWGLNYDYLDPYLVRPVSIGYVEYTPT
PIRYGIANFLANLDEPSSMINNLLMGNGNMAMVHFNRFWLNTTLGLVGLIDIASAADIQKPDSKSFGDALGHYGVGNGPY
FMLPGYGPWTLREAGDFVDGLYMPLSYLNFWQGLGKWGLEGMESRVQLISQEPMLEASPDPYVLTRDVYLQRQDFKAEIE
PEVDLDAEDDIDDYLDEIDE
>Mature_260_residues
MKRVWISCCILLFVSACSQTPEQTEEELDTTQQIDTEYEETHVSDPLEGFNRVMWGLNYDYLDPYLVRPVSIGYVEYTPT
PIRYGIANFLANLDEPSSMINNLLMGNGNMAMVHFNRFWLNTTLGLVGLIDIASAADIQKPDSKSFGDALGHYGVGNGPY
FMLPGYGPWTLREAGDFVDGLYMPLSYLNFWQGLGKWGLEGMESRVQLISQEPMLEASPDPYVLTRDVYLQRQDFKAEIE
PEVDLDAEDDIDDYLDEIDE

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=223, Percent_Identity=48.4304932735426, Blast_Score=215, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 29533; Mature: 29533

Theoretical pI: Translated: 3.81; Mature: 3.81

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVWISCCILLFVSACSQTPEQTEEELDTTQQIDTEYEETHVSDPLEGFNRVMWGLNYD
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCCCCC
YLDPYLVRPVSIGYVEYTPTPIRYGIANFLANLDEPSSMINNLLMGNGNMAMVHFNRFWL
CCCCHHCCEEEECEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEHHHHH
NTTLGLVGLIDIASAADIQKPDSKSFGDALGHYGVGNGPYFMLPGYGPWTLREAGDFVDG
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHH
LYMPLSYLNFWQGLGKWGLEGMESRVQLISQEPMLEASPDPYVLTRDVYLQRQDFKAEIE
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHCCCCCCCCCC
PEVDLDAEDDIDDYLDEIDE
CCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKRVWISCCILLFVSACSQTPEQTEEELDTTQQIDTEYEETHVSDPLEGFNRVMWGLNYD
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCCCCC
YLDPYLVRPVSIGYVEYTPTPIRYGIANFLANLDEPSSMINNLLMGNGNMAMVHFNRFWL
CCCCHHCCEEEECEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEHHHHH
NTTLGLVGLIDIASAADIQKPDSKSFGDALGHYGVGNGPYFMLPGYGPWTLREAGDFVDG
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHH
LYMPLSYLNFWQGLGKWGLEGMESRVQLISQEPMLEASPDPYVLTRDVYLQRQDFKAEIE
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHCCCCCCCCCC
PEVDLDAEDDIDDYLDEIDE
CCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]