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The map label for this gene is fadI

Identifier: 59712418

GI number: 59712418

Start: 2044812

End: 2046140

Strand: Reverse

Name: fadI

Synonym: VF_1811

Alternate gene names: 59712418

Gene position: 2046140-2044812 (Counterclockwise)

Preceding gene: 59712422

Following gene: 59712417

Centisome position: 70.62

GC content: 39.95

Gene sequence:

>1329_bases
ATGTCAAACAGCGTAAATTCAAGTAAGTATCAACCGTTAACGACACGACAAGGTGACCGTATTGCTGTTGTATCAGGTAT
TCGAACTCCGTTTGCTAAACAATCGACAGCATTCAGCACTACACCAGCTGTAGATCTTGGTAAGTTAGCGGTAAAAGCAT
TAATGGATAAAACGGATATTGATCCTAAATTAATAGATCAAGTCGTGTTTGGCCAAGTGGTACAAATGCCAGAAGCTCCG
AATATTGCTCGCGAAATTGTTTTGGGTACGGGGATGAATATAGGTACTGATGCTTATAGTGTTACTAGAGCTTGTGCGAC
GAGCTTTCAAACTACAGCTAATGTTGTGGAGAGTATTATGGCAGGCACGATTGATATTGGTATTGCTGGTGGTGCAGATT
CTTCCTCTGTATTGCCCATTGGTGTCTCTAAAAAGTTAGCTTCTACTTTACTTGCTCTTAGTAAAACAAAAACCGTTTAC
CAAAAGTTATCACTTCTTCGCACTTTATCATTAAAAGACATTGCTCCTGTTCCTCCTGCTGTCGCTGAATATTCAACGGG
AATTTCTATGGGACAAACAGCAGAACAAATGGCTAAAAGTCATGGAATAACACGTGAAGAACAAGATGCATTAGCACATC
GTTCACATACTCTTGCTGCCAAAGCATGGAAAGATGGGCTTATTCAAGATGAAGTAATGACCGCTTTCCCTGAACCTTAT
ACCGCATGGTTAGATCATGATAATAATATTCGTCACGATTCAGATCTAGCAAGTTATGCCAAATTACGCCCTGCATTTGA
TCATAAATATGGTTCAGTCACTGCAGCTAATAGTACGCCATTAACAGATGGTGGCGCCGCTTTATTATTAATGAGTGAAA
AACGAGCAAAAGAATTAGGTTATGAGCCATTAGGTTATATTCGTTCATTTGCGTTCAGTGCGATAGATGTGCATCACGAT
ATGTTAATGGGACCATCTTATGCGACGCCAATGGCCTTGGATAAAGCGGGAATTTCATTATCAGACCTTACCTTAATTGA
TATGCATGAAGCTTTTGCAGCACAAACCCTTTCAAATGTGAAAATGTTTGCTTCAAACAAATTTGCTAAAGAATATTTAG
GTAGAGATAAAGCCATTGGCGAAATTGATATGGAGAAATTTAATGTCCTTGGTGGGTCAATAGCCTATGGGCATCCATTT
GCAGCAACAGGAGCCAGAATGATTATTCAAACATTACGAGAATTAAAACGTCGTGGTGGTGGATTAGGATTAAATACGGC
TTGTGCCGCTGGTGGTTTAGGTGCAGCAATGGTGTTGGAGGTTGAATAA

Upstream 100 bases:

>100_bases
ATTATTCTATGATGAGCCTGTGATTATCATCACTATATCTTATTAACAACTTGGTTACAATTTGAGGTCTGACCACCTTT
GATATATGCAAGGAGAAGCA

Downstream 100 bases:

>100_bases
TGACGTTAGAAACGAAAAATAATACGCTTGAAGCCGTTAAAAATACCAGTGCATTTGCATGGACTAAAGATGATGAGCAA
ATTGCATGGTTAACCATCGA

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSNSVNSSKYQPLTTRQGDRIAVVSGIRTPFAKQSTAFSTTPAVDLGKLAVKALMDKTDIDPKLIDQVVFGQVVQMPEAP
NIAREIVLGTGMNIGTDAYSVTRACATSFQTTANVVESIMAGTIDIGIAGGADSSSVLPIGVSKKLASTLLALSKTKTVY
QKLSLLRTLSLKDIAPVPPAVAEYSTGISMGQTAEQMAKSHGITREEQDALAHRSHTLAAKAWKDGLIQDEVMTAFPEPY
TAWLDHDNNIRHDSDLASYAKLRPAFDHKYGSVTAANSTPLTDGGAALLLMSEKRAKELGYEPLGYIRSFAFSAIDVHHD
MLMGPSYATPMALDKAGISLSDLTLIDMHEAFAAQTLSNVKMFASNKFAKEYLGRDKAIGEIDMEKFNVLGGSIAYGHPF
AATGARMIIQTLRELKRRGGGLGLNTACAAGGLGAAMVLEVE

Sequences:

>Translated_442_residues
MSNSVNSSKYQPLTTRQGDRIAVVSGIRTPFAKQSTAFSTTPAVDLGKLAVKALMDKTDIDPKLIDQVVFGQVVQMPEAP
NIAREIVLGTGMNIGTDAYSVTRACATSFQTTANVVESIMAGTIDIGIAGGADSSSVLPIGVSKKLASTLLALSKTKTVY
QKLSLLRTLSLKDIAPVPPAVAEYSTGISMGQTAEQMAKSHGITREEQDALAHRSHTLAAKAWKDGLIQDEVMTAFPEPY
TAWLDHDNNIRHDSDLASYAKLRPAFDHKYGSVTAANSTPLTDGGAALLLMSEKRAKELGYEPLGYIRSFAFSAIDVHHD
MLMGPSYATPMALDKAGISLSDLTLIDMHEAFAAQTLSNVKMFASNKFAKEYLGRDKAIGEIDMEKFNVLGGSIAYGHPF
AATGARMIIQTLRELKRRGGGLGLNTACAAGGLGAAMVLEVE
>Mature_441_residues
SNSVNSSKYQPLTTRQGDRIAVVSGIRTPFAKQSTAFSTTPAVDLGKLAVKALMDKTDIDPKLIDQVVFGQVVQMPEAPN
IAREIVLGTGMNIGTDAYSVTRACATSFQTTANVVESIMAGTIDIGIAGGADSSSVLPIGVSKKLASTLLALSKTKTVYQ
KLSLLRTLSLKDIAPVPPAVAEYSTGISMGQTAEQMAKSHGITREEQDALAHRSHTLAAKAWKDGLIQDEVMTAFPEPYT
AWLDHDNNIRHDSDLASYAKLRPAFDHKYGSVTAANSTPLTDGGAALLLMSEKRAKELGYEPLGYIRSFAFSAIDVHHDM
LMGPSYATPMALDKAGISLSDLTLIDMHEAFAAQTLSNVKMFASNKFAKEYLGRDKAIGEIDMEKFNVLGGSIAYGHPFA
ATGARMIIQTLRELKRRGGGLGLNTACAAGGLGAAMVLEVE

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family

Homologues:

Organism=Homo sapiens, GI4504327, Length=420, Percent_Identity=41.6666666666667, Blast_Score=340, Evalue=2e-93,
Organism=Homo sapiens, GI4557237, Length=426, Percent_Identity=30.9859154929577, Blast_Score=195, Evalue=8e-50,
Organism=Homo sapiens, GI4501853, Length=430, Percent_Identity=31.1627906976744, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI167614485, Length=423, Percent_Identity=30.2600472813239, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI148539872, Length=428, Percent_Identity=29.4392523364486, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI194353979, Length=106, Percent_Identity=37.7358490566038, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788683, Length=431, Percent_Identity=71.461716937355, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI87082165, Length=427, Percent_Identity=33.0210772833724, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1788554, Length=420, Percent_Identity=34.2857142857143, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI48994986, Length=428, Percent_Identity=32.9439252336449, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1787663, Length=423, Percent_Identity=30.7328605200946, Blast_Score=171, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17551802, Length=420, Percent_Identity=38.3333333333333, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI133906874, Length=427, Percent_Identity=32.3185011709602, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI25147385, Length=421, Percent_Identity=26.8408551068884, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17535921, Length=429, Percent_Identity=27.972027972028, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17535917, Length=428, Percent_Identity=21.2616822429907, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325229, Length=433, Percent_Identity=29.3302540415704, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322031, Length=385, Percent_Identity=29.6103896103896, Blast_Score=153, Evalue=4e-38,
Organism=Drosophila melanogaster, GI17137578, Length=435, Percent_Identity=41.8390804597701, Blast_Score=331, Evalue=5e-91,
Organism=Drosophila melanogaster, GI24655093, Length=422, Percent_Identity=28.9099526066351, Blast_Score=171, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24640423, Length=426, Percent_Identity=28.169014084507, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI17648125, Length=381, Percent_Identity=28.3464566929134, Blast_Score=138, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FADI_VIBF1 (Q5E3U0)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205194.1
- ProteinModelPortal:   Q5E3U0
- SMR:   Q5E3U0
- STRING:   Q5E3U0
- GeneID:   3279084
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1811
- NMPDR:   fig|312309.3.peg.1810
- eggNOG:   COG0183
- HOGENOM:   HBG370930
- OMA:   RDEVMTA
- PhylomeDB:   Q5E3U0
- ProtClustDB:   PRK08963
- BioCyc:   VFIS312309:VF1811-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01618
- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616
- Gene3D:   G3DSA:3.40.47.10
- PANTHER:   PTHR18919
- PIRSF:   PIRSF000429
- TIGRFAMs:   TIGR01930
- TIGRFAMs:   TIGR02446

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N; SSF53901 Thiolase-like

EC number: =2.3.1.16

Molecular weight: Translated: 47077; Mature: 46946

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00098 THIOLASE_1; PS00737 THIOLASE_2; PS00099 THIOLASE_3

Important sites: ACT_SITE 105-105 ACT_SITE 398-398 ACT_SITE 428-428

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSVNSSKYQPLTTRQGDRIAVVSGIRTPFAKQSTAFSTTPAVDLGKLAVKALMDKTDI
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
DPKLIDQVVFGQVVQMPEAPNIAREIVLGTGMNIGTDAYSVTRACATSFQTTANVVESIM
CHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AGTIDIGIAGGADSSSVLPIGVSKKLASTLLALSKTKTVYQKLSLLRTLSLKDIAPVPPA
HCCEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCH
VAEYSTGISMGQTAEQMAKSHGITREEQDALAHRSHTLAAKAWKDGLIQDEVMTAFPEPY
HHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
TAWLDHDNNIRHDSDLASYAKLRPAFDHKYGSVTAANSTPLTDGGAALLLMSEKRAKELG
EEEECCCCCCCCCHHHHHHHHHCHHHCCCCCCEEECCCCCCCCCCCEEEEECHHHHHHCC
YEPLGYIRSFAFSAIDVHHDMLMGPSYATPMALDKAGISLSDLTLIDMHEAFAAQTLSNV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHEEEEHHHHHHHHHHHHHH
KMFASNKFAKEYLGRDKAIGEIDMEKFNVLGGSIAYGHPFAATGARMIIQTLRELKRRGG
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
GLGLNTACAAGGLGAAMVLEVE
CCCCCHHHHCCCCCEEEEEEEC
>Mature Secondary Structure 
SNSVNSSKYQPLTTRQGDRIAVVSGIRTPFAKQSTAFSTTPAVDLGKLAVKALMDKTDI
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
DPKLIDQVVFGQVVQMPEAPNIAREIVLGTGMNIGTDAYSVTRACATSFQTTANVVESIM
CHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AGTIDIGIAGGADSSSVLPIGVSKKLASTLLALSKTKTVYQKLSLLRTLSLKDIAPVPPA
HCCEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCH
VAEYSTGISMGQTAEQMAKSHGITREEQDALAHRSHTLAAKAWKDGLIQDEVMTAFPEPY
HHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
TAWLDHDNNIRHDSDLASYAKLRPAFDHKYGSVTAANSTPLTDGGAALLLMSEKRAKELG
EEEECCCCCCCCCHHHHHHHHHCHHHCCCCCCEEECCCCCCCCCCCEEEEECHHHHHHCC
YEPLGYIRSFAFSAIDVHHDMLMGPSYATPMALDKAGISLSDLTLIDMHEAFAAQTLSNV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHEEEEHHHHHHHHHHHHHH
KMFASNKFAKEYLGRDKAIGEIDMEKFNVLGGSIAYGHPFAATGARMIIQTLRELKRRGG
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
GLGLNTACAAGGLGAAMVLEVE
CCCCCHHHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA