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The map label for this gene is msbB [H]

Identifier: 59712406

GI number: 59712406

Start: 2031969

End: 2032910

Strand: Reverse

Name: msbB [H]

Synonym: VF_1799

Alternate gene names: 59712406

Gene position: 2032910-2031969 (Counterclockwise)

Preceding gene: 59712408

Following gene: 59712405

Centisome position: 70.16

GC content: 38.32

Gene sequence:

>942_bases
ATGACTGAAAAAATCGATAAAAACGTTTATAACCCAACATTTCAATGGGCATTTCTCCATCCTAAATACTGGCCTACGTG
GCTTGGGATTGGTTTTGCTATTATTTTGGCTTTTATTCCATTTCGATTACGTGACAAATTAGCAGCGAAAATCGTTAAAT
TAGTACTTAAGAAACCAAGTGGTGGTATTAAGCGTGCTAAGTTAAATATTGCGACCTGTTTTCCTGAAAAATCAGAACAA
GAGCAACAGAAACTGCTTGAAGATACTTATATTACGGGTGCTCAAGTTATGCTTGGCTTTTCTGAGTTGCTTGTTCGTAG
CCGTAAGCATAATGAAAAACGCGGTATTTTTGTGGGAGGAGAGAATTTATTTCCTCTTCTTGAGCAGAAAGAAAACATCA
TTATTTTAGCACCTCATGCATGGGCTATTGATTTTCCAGCTATCTTATTAGCTTCTCGTGGTTATCATGTAACCACAATG
ATGAAACCACAAAAAAACCCAATTACAGACTGGCTAATGCACGTTCAGCGAATGCAATATGGCGGTAAGATCTATGCTCG
TAGTGCGGGTATTAAACCGTTAATTAAAGCAATTAAAGAAGGTTATTTAGCGTATTACCTACCAGATGAAGATCATGGTC
CTCAAAATAGTGTATTTGTACCTTTCTTTGCGACTCAGAAAGCGACGCTAAAAGGGTTTGGTAAATTAGCTCGCTTATCA
AAAGCGAAAGTAGTGCCAATGTTACCATCTTATAACTCTGAAACGGGTCAGTTTGAAATTATTACCTTACCAGCTATTGC
TGATTTTCCATCTGGTGATGAAGAAACAGATGCTCGCGCGATGAATATTGCTATTGAGCAGCTTGTGACTGAGCGTCCTG
AACAATATATGTGGGTATTAAACTTACTACGCTCTCGTCCAGGTGGTAGCCGTTTGTATTAA

Upstream 100 bases:

>100_bases
GTGGGTGTAATTGGAAAGCCATCCCTATCTCCTAGGTTTCTATCAGCAATGCTTTGTTATATCATAACTCACTTATAATA
ATGAGACAGTGAAGCAATTC

Downstream 100 bases:

>100_bases
CTTTTTTGTTATTCGGCTCTTATTTGAAACCCGTAGAATTACACAGTTCTGCGGGTTTTTTGTGTCTCATAGAAATTTTT
GTTATTCGTTTTGTAACAGA

Product: lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase

Products: KDO2-lipid A, cold adapted; [acyl carrier protein]; KDO2-lipid A [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTEKIDKNVYNPTFQWAFLHPKYWPTWLGIGFAIILAFIPFRLRDKLAAKIVKLVLKKPSGGIKRAKLNIATCFPEKSEQ
EQQKLLEDTYITGAQVMLGFSELLVRSRKHNEKRGIFVGGENLFPLLEQKENIIILAPHAWAIDFPAILLASRGYHVTTM
MKPQKNPITDWLMHVQRMQYGGKIYARSAGIKPLIKAIKEGYLAYYLPDEDHGPQNSVFVPFFATQKATLKGFGKLARLS
KAKVVPMLPSYNSETGQFEIITLPAIADFPSGDEETDARAMNIAIEQLVTERPEQYMWVLNLLRSRPGGSRLY

Sequences:

>Translated_313_residues
MTEKIDKNVYNPTFQWAFLHPKYWPTWLGIGFAIILAFIPFRLRDKLAAKIVKLVLKKPSGGIKRAKLNIATCFPEKSEQ
EQQKLLEDTYITGAQVMLGFSELLVRSRKHNEKRGIFVGGENLFPLLEQKENIIILAPHAWAIDFPAILLASRGYHVTTM
MKPQKNPITDWLMHVQRMQYGGKIYARSAGIKPLIKAIKEGYLAYYLPDEDHGPQNSVFVPFFATQKATLKGFGKLARLS
KAKVVPMLPSYNSETGQFEIITLPAIADFPSGDEETDARAMNIAIEQLVTERPEQYMWVLNLLRSRPGGSRLY
>Mature_312_residues
TEKIDKNVYNPTFQWAFLHPKYWPTWLGIGFAIILAFIPFRLRDKLAAKIVKLVLKKPSGGIKRAKLNIATCFPEKSEQE
QQKLLEDTYITGAQVMLGFSELLVRSRKHNEKRGIFVGGENLFPLLEQKENIIILAPHAWAIDFPAILLASRGYHVTTMM
KPQKNPITDWLMHVQRMQYGGKIYARSAGIKPLIKAIKEGYLAYYLPDEDHGPQNSVFVPFFATQKATLKGFGKLARLSK
AKVVPMLPSYNSETGQFEIITLPAIADFPSGDEETDARAMNIAIEQLVTERPEQYMWVLNLLRSRPGGSRLY

Specific function: Transfers myristate or laurate, activated on ACP, to (KDO)2-(lauroyl)-lipid IVA [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1788161, Length=310, Percent_Identity=38.3870967741935, Blast_Score=257, Evalue=8e-70,
Organism=Escherichia coli, GI1787292, Length=303, Percent_Identity=32.3432343234323, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI87082094, Length=306, Percent_Identity=26.4705882352941, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011921 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 35569; Mature: 35438

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKIDKNVYNPTFQWAFLHPKYWPTWLGIGFAIILAFIPFRLRDKLAAKIVKLVLKKPS
CCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GGIKRAKLNIATCFPEKSEQEQQKLLEDTYITGAQVMLGFSELLVRSRKHNEKRGIFVGG
CCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEECC
ENLFPLLEQKENIIILAPHAWAIDFPAILLASRGYHVTTMMKPQKNPITDWLMHVQRMQY
CCCCHHHCCCCCEEEECCCCCHHCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
GGKIYARSAGIKPLIKAIKEGYLAYYLPDEDHGPQNSVFVPFFATQKATLKGFGKLARLS
CCEEEEECCCCHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
KAKVVPMLPSYNSETGQFEIITLPAIADFPSGDEETDARAMNIAIEQLVTERPEQYMWVL
HCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
NLLRSRPGGSRLY
HHHHCCCCCCCCC
>Mature Secondary Structure 
TEKIDKNVYNPTFQWAFLHPKYWPTWLGIGFAIILAFIPFRLRDKLAAKIVKLVLKKPS
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GGIKRAKLNIATCFPEKSEQEQQKLLEDTYITGAQVMLGFSELLVRSRKHNEKRGIFVGG
CCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEECC
ENLFPLLEQKENIIILAPHAWAIDFPAILLASRGYHVTTMMKPQKNPITDWLMHVQRMQY
CCCCHHHCCCCCEEEECCCCCHHCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
GGKIYARSAGIKPLIKAIKEGYLAYYLPDEDHGPQNSVFVPFFATQKATLKGFGKLARLS
CCEEEEECCCCHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
KAKVVPMLPSYNSETGQFEIITLPAIADFPSGDEETDARAMNIAIEQLVTERPEQYMWVL
HCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
NLLRSRPGGSRLY
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO2-(palmitoleoyl)-lipid IVA; myristoyl-ACP; KDO2-(lauroyl)-lipid IVA [C]

Specific reaction: KDO2-(palmitoleoyl)-lipid IVA + myristoyl-ACP = KDO2-lipid A, cold adapted + [acyl carrier protein] KDO2-(lauroyl)-lipid IVA + myristoyl-ACP = KDO2-lipid A + [acyl carrier protein] [C]

General reaction: Transferases; Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]