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The map label for this gene is purB [H]

Identifier: 59712393

GI number: 59712393

Start: 2015762

End: 2017132

Strand: Direct

Name: purB [H]

Synonym: VF_1786

Alternate gene names: 59712393

Gene position: 2015762-2017132 (Clockwise)

Preceding gene: 59712392

Following gene: 172087728

Centisome position: 69.57

GC content: 41.94

Gene sequence:

>1371_bases
ATGGAATTGTCAGCATTAACTGCTGTTTCACCTGTAGATGGTCGCTACGGAAGTAAAACAATTTCTCTACGCAGCATCTT
TAGTGAGTATGGCTTACTAAAGTACCGTACTGTTGTTGAAGTTCGTTGGCTTCAAAAACTAGCTGCTACGCAAACGATTG
CGGAAGTAGCTACATTAAGCGCAGAAGCGAATCAATTCTTAGATAACATCGCAGCAAACTTCAATGAAGAAGATGCAATG
CGTATCAAAGAAATTGAACGCACTACAAACCATGACGTTAAAGCAGTTGAATACTTTCTAAAAGAGAAAGTAGCTGAAGT
TCCAGAACTTCACGCTATTAATGAATTCATTCACTTTGCATGTACTTCAGAAGATATCAACAACACGTCTCACGCTCTAA
TGCTTAAAGAAGCTCGTGATACGGTAATTCTTCCAGAAATTCGTAACGTTATTGATGCGATTAAAGCATTAGCAAACGAA
TACCGTGACATTCCACTTCTGTCTCGTACACACGGTCAACCAGCTTCTCCATCGACTATGGGTAAAGAAATGGCTAACGT
GGCGTACCGTATGGAACGTCAATACAAGCAAATCGAAAACGTTGAAATCCTAGCTAAAATCAATGGTGCGGTTGGTAACT
ATAACGCTCACCTTTCTGCTTACCCAGATGTTGATTGGCACAAATTCAGCGAAGAGTTCATCACTGAGTCTCTAGGTGTT
ACTTGGAACCCATACACAACTCAAATCGAACCTCACGATTACATCGCTGAGCTATTTGATGCGATTGCTCGTTTTAATAC
TATCCTTCTTGATTTTGACCGTGACGTATGGGGCTACATTGCTCTTGGCCACTTCAAGCAAAAAACAATTGCTGGTGAAA
TCGGTTCTTCAACAATGCCGCACAAAGTTAACCCAATCGACTTTGAAAACTCAGAAGGTAACCTAGGTCTTGCTAATGCT
ATCTTTAGCCACCTAGCACAAAAACTGCCTGTATCTCGCTGGCAGCGTGATCTAACTGACTCAACGGTTCTTCGTAACCT
AGGTGTTGGTTGTGGCTACGCTATCATTGCATACACATCGACTCTAAAAGGCATTAGCAAATTAGAAGTTAACCGTGATG
CTCTACTTGCTGAGCTAGATAAAAACTGGGAAGTTCTTGCTGAACCTGTACAAACAGTAATGCGTCGTTACGGCATCGAA
AAGCCATACGAAAAACTAAAAGAACTGACTCGTGGTAAGCGTGTAGATGGCGAAGGCATGCGTGCATTCATCGACGGTTT
AGAAATCCCTGCAGATGAGAAAGCTCGTCTAAAAGAGATGACTCCAGCTAACTACATCGGTCAAGCTATCGAGCTAACTG
ACAAACTGTAA

Upstream 100 bases:

>100_bases
GCAAGCAAAAATTATTCTCGCTCAAATTTAAAAAATTAAAGCCTACTTGATTTCAGGTAGGCTTTTTCCTTTAACATTTA
TACATATCTAGGAGATTCAC

Downstream 100 bases:

>100_bases
TCGCTACAGTAAACCATTTAAAAGGCTGGCATTTTGTCGGCCTTTTTTATTACCTCTCATATAAAACCTAAAGGCTACTA
AACTTCTTCCTGTATTGATT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MELSALTAVSPVDGRYGSKTISLRSIFSEYGLLKYRTVVEVRWLQKLAATQTIAEVATLSAEANQFLDNIAANFNEEDAM
RIKEIERTTNHDVKAVEYFLKEKVAEVPELHAINEFIHFACTSEDINNTSHALMLKEARDTVILPEIRNVIDAIKALANE
YRDIPLLSRTHGQPASPSTMGKEMANVAYRMERQYKQIENVEILAKINGAVGNYNAHLSAYPDVDWHKFSEEFITESLGV
TWNPYTTQIEPHDYIAELFDAIARFNTILLDFDRDVWGYIALGHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLANA
IFSHLAQKLPVSRWQRDLTDSTVLRNLGVGCGYAIIAYTSTLKGISKLEVNRDALLAELDKNWEVLAEPVQTVMRRYGIE
KPYEKLKELTRGKRVDGEGMRAFIDGLEIPADEKARLKEMTPANYIGQAIELTDKL

Sequences:

>Translated_456_residues
MELSALTAVSPVDGRYGSKTISLRSIFSEYGLLKYRTVVEVRWLQKLAATQTIAEVATLSAEANQFLDNIAANFNEEDAM
RIKEIERTTNHDVKAVEYFLKEKVAEVPELHAINEFIHFACTSEDINNTSHALMLKEARDTVILPEIRNVIDAIKALANE
YRDIPLLSRTHGQPASPSTMGKEMANVAYRMERQYKQIENVEILAKINGAVGNYNAHLSAYPDVDWHKFSEEFITESLGV
TWNPYTTQIEPHDYIAELFDAIARFNTILLDFDRDVWGYIALGHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLANA
IFSHLAQKLPVSRWQRDLTDSTVLRNLGVGCGYAIIAYTSTLKGISKLEVNRDALLAELDKNWEVLAEPVQTVMRRYGIE
KPYEKLKELTRGKRVDGEGMRAFIDGLEIPADEKARLKEMTPANYIGQAIELTDKL
>Mature_456_residues
MELSALTAVSPVDGRYGSKTISLRSIFSEYGLLKYRTVVEVRWLQKLAATQTIAEVATLSAEANQFLDNIAANFNEEDAM
RIKEIERTTNHDVKAVEYFLKEKVAEVPELHAINEFIHFACTSEDINNTSHALMLKEARDTVILPEIRNVIDAIKALANE
YRDIPLLSRTHGQPASPSTMGKEMANVAYRMERQYKQIENVEILAKINGAVGNYNAHLSAYPDVDWHKFSEEFITESLGV
TWNPYTTQIEPHDYIAELFDAIARFNTILLDFDRDVWGYIALGHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLANA
IFSHLAQKLPVSRWQRDLTDSTVLRNLGVGCGYAIIAYTSTLKGISKLEVNRDALLAELDKNWEVLAEPVQTVMRRYGIE
KPYEKLKELTRGKRVDGEGMRAFIDGLEIPADEKARLKEMTPANYIGQAIELTDKL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI183227688, Length=347, Percent_Identity=24.4956772334294, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI4557269, Length=347, Percent_Identity=24.4956772334294, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1787376, Length=456, Percent_Identity=81.140350877193, Blast_Score=782, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323391, Length=346, Percent_Identity=24.2774566473988, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24647570, Length=348, Percent_Identity=25.8620689655172, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51387; Mature: 51387

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSALTAVSPVDGRYGSKTISLRSIFSEYGLLKYRTVVEVRWLQKLAATQTIAEVATLS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEANQFLDNIAANFNEEDAMRIKEIERTTNHDVKAVEYFLKEKVAEVPELHAINEFIHFA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
CTSEDINNTSHALMLKEARDTVILPEIRNVIDAIKALANEYRDIPLLSRTHGQPASPSTM
CCCCCCCCCHHHEEEECCCCCEECHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHH
GKEMANVAYRMERQYKQIENVEILAKINGAVGNYNAHLSAYPDVDWHKFSEEFITESLGV
HHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCC
TWNPYTTQIEPHDYIAELFDAIARFNTILLDFDRDVWGYIALGHFKQKTIAGEIGSSTMP
CCCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
HKVNPIDFENSEGNLGLANAIFSHLAQKLPVSRWQRDLTDSTVLRNLGVGCGYAIIAYTS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
TLKGISKLEVNRDALLAELDKNWEVLAEPVQTVMRRYGIEKPYEKLKELTRGKRVDGEGM
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHH
RAFIDGLEIPADEKARLKEMTPANYIGQAIELTDKL
HHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MELSALTAVSPVDGRYGSKTISLRSIFSEYGLLKYRTVVEVRWLQKLAATQTIAEVATLS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEANQFLDNIAANFNEEDAMRIKEIERTTNHDVKAVEYFLKEKVAEVPELHAINEFIHFA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
CTSEDINNTSHALMLKEARDTVILPEIRNVIDAIKALANEYRDIPLLSRTHGQPASPSTM
CCCCCCCCCHHHEEEECCCCCEECHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHH
GKEMANVAYRMERQYKQIENVEILAKINGAVGNYNAHLSAYPDVDWHKFSEEFITESLGV
HHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCC
TWNPYTTQIEPHDYIAELFDAIARFNTILLDFDRDVWGYIALGHFKQKTIAGEIGSSTMP
CCCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
HKVNPIDFENSEGNLGLANAIFSHLAQKLPVSRWQRDLTDSTVLRNLGVGCGYAIIAYTS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
TLKGISKLEVNRDALLAELDKNWEVLAEPVQTVMRRYGIEKPYEKLKELTRGKRVDGEGM
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHH
RAFIDGLEIPADEKARLKEMTPANYIGQAIELTDKL
HHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]